stability of lycopene in tomato dehydration

Báo cáo khoa học: Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus pot

Ngày tải lên : 16/03/2014, 05:20

... (CrtISO in plants and CrtH in cyanobacteria) is used for cis-totrans isomerization, converting cis -lycopene into trans- In M xanthus, a mutant with a transposon insertion in the coding region of crtIb ... operon includes the last ﬁve genes of the cluster, and its transcription is driven by a light-independent promoter, PA in- frame deletion of crtIa in M xanthus strain MR151 This strain contains a ... f-carotene, in decreasing concentrations (Table 1) This pattern of carotene accumulation, notably similar to that resulting from the heterologous expression of crtIa in E coli (strain FD6 in Table 1), indicates...

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Báo cáo sinh học: " Detection and frequency of recombination in tomato-infecting begomoviruses of South and Southeast Asia" pptx

Ngày tải lên : 18/06/2014, 18:20

... evolving Although a few specific recombination events have been described so far in tomato- infecting begomoviruses [2629], a full accounting of recombinants, recombination breakpoints and recombination ... North India South India Thailand Bangladesh East & West India China Malaysia, Taiwan & Philippines Sri Lanka Figure sequences TreeOrder Scan of tomato- infecting begomoviruses TreeOrder Scan of tomato- infecting ... recombinant forms, recombination hot spots and frequency of recombination documented in this study would provide new information for understanding the diversity and evolution of tomato- infecting...

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Báo cáo hóa học: " Detection and frequency of recombination in tomato-infecting begomoviruses of South and Southeast Asia" pptx

Ngày tải lên : 20/06/2014, 01:20

... evolving Although a few specific recombination events have been described so far in tomato- infecting begomoviruses [2629], a full accounting of recombinants, recombination breakpoints and recombination ... North India South India Thailand Bangladesh East & West India China Malaysia, Taiwan & Philippines Sri Lanka Figure sequences TreeOrder Scan of tomato- infecting begomoviruses TreeOrder Scan of tomato- infecting ... recombinant forms, recombination hot spots and frequency of recombination documented in this study would provide new information for understanding the diversity and evolution of tomato- infecting...

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Báo cáo khoa hoc:" Formation and stability of oil-in-water nanoemulsions containing rice bran oil: in vitro and in vivo assessments" pdf

Ngày tải lên : 11/08/2014, 08:20

... variance in the first 30 minutes and maintained this increase up to 90 minutes after application Then, the moisturising capacitance decreased after 90 minutes until the end of test (Figure 6) Skin ... compared with healthy skin People with these drying skin conditions have increased dryness in their The oiliness values of nanoemulsion-treated skin increased considerably 30 minutes after treatment ... desirable in dry skin conditions [42] Therefore, the oiliness of the nanoemulsion may provide an alternative treatment for psoriasis Skin pH determination Forearm skin testing is standard in most clinical...

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An approximate method for investigation of the stability of motion in the critical case

Ngày tải lên : 08/04/2015, 15:36

... (1), lying on the boundary of the stability, to the equivalent linear form (2 ) we use this assumption, i.e that the motionof theconsi dered, system in the neigbourhood of the boundary of stability ... tio n in th e C ritica l C ase 21 Substituting y % from (19) into the right-hand side of Eq (18) and lincarizating y it, we find: y+y=ey/ {a [r C S 0+2ey/ r2( —fi sin 20+'y C S 29)]-b [r sin 0-fO ... with higher order in expansions (4) However* in practice it often requires the knowledge only of the form of solution in the first a p p r o x im a tio n x = r COS Ớ, y = r sin o r in th e s e c...

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Tài liệu Báo cáo " ON THE STABILITY OF ELASTOPLASTIC THIN TRIANGULAR PLATES MADE IN COMPRESSIBLE MATERIAL " doc

Ngày tải lên : 13/02/2014, 04:20

... Plastic deformation and instability in thin-Walled turber under combined loading: a general theory, Journal of Mech of Solids, 47(1999), pp 921-933 Dao Van Dung, Stability of thin plates with compressible ... On the stability of elastoplastic thin triangular plates made in 15 of the strain velocity tensor detemined by the stress-strain relationship of elastoplastic process theory [1, 7] are of the ... hardening material, s = φ−1 (p) nn1.2 Post-buckling state At the moment an instability occurs, a bifurcation of equilibrium states is assumed to appear The system of stability equations of the...

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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc

Ngày tải lên : 19/02/2014, 12:20

... formation of cavities, one in the hydrophobic core (M88V) and the other in the protein–RNA interaction (T45S), decrease the stability of the capsid Our ﬁndings illuminate the role of packing in the ... tool for using to study cavities in proteins [24,25] The coat protein of bacteriophage MS2 contains two tryptophan residues, thus permitting the use of intrinsic ﬂuorescence as a probe of structural ... Asymmetric interactions in the adenosine-binding pockets of the MS2 coat protein dimer BMC Mol Biol 2, 48 Lima, L.M.T., Foguel, D & Silva, J.L (2000) DNA tightens the dimeric DNA-binding domain of human...

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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

Ngày tải lên : 20/02/2014, 23:20

... be involved in changing the thermally stable characteristics of vertebrate DNases I, substitution of Ile130Leu in snakes and insertion of Ser205 in amphibia It is interesting that DNases I of ... deoxyribonuclease isolated from bovine, ovine, and porcine pancreas Derivation of the amino acid sequence of ovine DNase and revision of the previously published amino acid sequence of bovine DNase J Biol Chem ... also found in all the snake enzymes The unique cysteine-rich-terminus and inserted Ser in the Ca2+-binding site of amphibian DNases I [14], were not observed in the snake enzymes As in mammalian,...

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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Ngày tải lên : 21/02/2014, 03:20

... amino group of the main chain at Glu268 The oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and Ala270 by hydrogen bonds The interactions should maintain ... stable retaining 60% of the original activity after h, and 30% after h The D266NALP I showed 40 and 20% of the original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- ... core structure of ALP I independently As the side chain of Asn266 would be able to bind to the main chain of Ile10 with a hydrogen bond by substitution of Asp266Asn, the mobility of the two regions...

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Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx

Ngày tải lên : 07/03/2014, 03:20

... domains, respectively, and appear to stabilize their protein scaffolds, allowing the proper positioning of the residues involved in PLP binding, substrate binding and modulation of the cofactor’s ... value determined from the amino acid sequence (45 320 Da) Measurement of the Kd of PLP binding equilibrium PLP binding equilibria were analyzed taking advantage of the protein intrinsic ﬂuorescence ... 139 Role of hydrophobic contacts in serine hydroxymethyltransferase to bind PLP In this intermediate, the N-terminus and an inter-domain segment remain exposed to solvent In the following, slow...

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Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

Ngày tải lên : 07/03/2014, 12:20

... Cristofaro et al Fig Kinetics of disulﬁde scrambling of WT and DK9 thrombin Kinetics of reduced A-chain release from WT (s) and DK9 thrombin (h) Continuous lines were drawn according to a single ... simulation of DK9 thrombin in explicit water solvent supports the role of the A-chain in affecting the conformation and catalytic properties of the B-chain, particularly in some insertion loops of the ... thrombin show that the A-chain closely follows the contour of the catalytic B-chain, hinging the two interacting six-stranded barrel-like domains of the B-chain [17] A well-structured network of...

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Báo cáo khoa học: Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating ﬁbrinolysis doc

Ngày tải lên : 07/03/2014, 12:20

... bradykinin using an arginine kinase-based kinetic assay [27] Data are presented in Table No differences were seen in the Km values of the three CPUs for bradykinin and Hip-Arg when the kinetic ... Development of a fast kinetic method for the determination of carboxypeptidase U (TAFIa) using C-terminal arginine containing peptides as substrate Anal Biochem 340, 106–112 28 Leurs J, Wissing BM, ... T1 ⁄ of their activity was determined (Table 2) These combinations increased the stability of CPU’s activity, especially the YQ mutant Following the ﬁrst round of FINDTM treatment, 50% of the...

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Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

Ngày tải lên : 16/03/2014, 10:20

... stabilizing the proteinprotein interface [31] All of the interactions reported in Fig in the range between and 6.0 A are identical in the major and minor fractions of the holoprotein in solution In ... overexpress in E coli, presumably due to inefciency of the E coli holo-ACP synthase in modifying the ACP in vivo, resulting in the production of mostly apo-ACP, which has been shown to have inhibitory ... modied serine [22] PfACP is a protein of 137 residues, inclusive of signal and transit sequences, required for targeting of the protein to the apicoplast The mature protein comprises 79 amino acids...

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Báo cáo khoa học: Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide ppt

Ngày tải lên : 16/03/2014, 13:20

... were introduced during the ﬁrst step of evolution Stability of mutant FDHs in PAA Finally, the performance of the FDH variants after entrapment in PAA was investigated by measuring the activity in ... temperature of 30 °C for 30 The reaction was started by injecting 100 lmol of formate into the vial and stopped after 60 by injecting mL of HCl (1 molÆL)1) After another 30 of incubation, a gas sample of ... replacement of glutamic acid in position 53, a lysine residue in position 56 was replaced by arginine As the 3D structure of FDH from C boidinii has not been solved to date, the distinct locations of...

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Báo cáo khoa học: Predicting the substrate speciﬁcity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit pptx

Ngày tải lên : 28/03/2014, 23:20

... maintain catalytically favourable binding geometries Docking of hydroquinone in the acceptor binding pocket of SlUGT5 resulted in a single conformation cluster (Fig 4A) in which the alcohol hydroxyl ... b-isosalicin as the reaction product Docking of salicyl alcohol into the acceptor binding pocket yielded three main binding clusters (Fig 4C) In cluster 1, the primary alcohol hydroxyl group of salicyl ... the b-isosalicin production determined by NMR The main difference between the conformation clusters lies in the position of the aromatic ring of salicyl alcohol in the binding pocket In clusters...

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Báo cáo khoa học: Engineering thermal stability of L-asparaginase by in vitro directed evolution ppt

Ngày tải lên : 30/03/2014, 02:20

... This incompatibility often relates to the stability of the enzymes under body conditions In the case of engineering proteins for thermostability, researchers are in the enviable position of being ... The rates of inactivation were followed by periodically removing samples for assay of enzymatic activity Observed rates of inactivation (kin) Engineering thermal stability of L-asparaginase were ... enzyme In principle, the entropy of inactivation consists of two parts: the increased conﬁgurational entropy due to partial unfolding of the protein in the transition state and the decrease in entropy...

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Báo cáo khoa học: Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved hydrophobic contacts potx

Ngày tải lên : 30/03/2014, 02:20

... directly involved in the proper positioning or stabilization of the active site or PLP-binding residues Its location rather suggests a role in bridging different structural regions of the protein in ... from the global nonlinear least squares ﬁtting of internal aldimine and activity data to Eqns (1,2) cofactor is bound to the enzyme as internal aldimine, indicating that the loss of activity does ... PLP-binding properties of the monomeric denaturation intermediate The cofactor binding properties of monomeric wildtype and mutant enzymes in 2.3 m urea (dissolved in the same buffer used in the...

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Báo cáo khoa học: Stability of the major allergen Brazil nut 2S albumin (Ber e 1) to physiologically relevant in vitro gastrointestinal digestion doc

Ngày tải lên : 30/03/2014, 15:20

... Uncertain origin a c Peptides resulting from speciﬁc cleavage of trypsin ⁄ chymotrypsin Peptides obtained during gastric digestion (phase 1) main IgE epitope region albumin (Fig 6B) is located in ... following in vitro gastric digestion, 25% of the allergen remained intact, whereas 50% corresponded to a ‘large fragment’ (Mr 6400) comprising mainly peptides matching with the large chain linked ... Uncertain origin Mass c Ragged C- and N-termini located in the large chain Therefore, a common IgE epitope has been described in the large chain of 2S albumins from yellow and oriental mustard Sin...

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Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx

Ngày tải lên : 30/03/2014, 20:20

... thermal stability was affected in a similar way Electrostatic interactions play a critical role in guiding and stabilizing functional protein–protein interactions [6], as well as in the structural stability ... ± ± ± ± ± stability of the protein, whereas inversion of the charge at position 72 considerably reduces the stability against urea-induced unfolding, i.e electrostatic interactions of the negative ... many proteins the dependence of DHU,m on Tm is linear, indicating an entropic origin of the differences in stability [30–32] The slope of such a representation corresponds to DCp,U In our case,...

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Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

Ngày tải lên : 30/03/2014, 20:20

... cycle [11–15] Certain transacting proteins such as HuR were proposed as regulators of changes in mRNA stability during the cell cycle [15] In this context it is of interest that during vertebrate ... thymidine ⁄ amphidicolin block, the half-life of cyclin B1 mRNA measured after actinomycin D addition increased from 1.2 h in early G1 to 12 h in G2 ⁄ M phase [11] With the same method the cyclin ... the estimated doubling time of EL4 cells in logarithmic growth No major variation in the mRNA half-life of cyclin mRNAs in the cell cycle In order to test whether changes in mRNA steadystate...

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