split horizon is disabled by default on frame relay physical interfaces

... kDa band was excised. The sample was analysed using Q-TRAP MS by the Protein Analysis Laboratory at the Cancer Research UK London Research Institute (London, UK). Immunoprecipitation Cells were ... was clarified by centrifugation at 15 000 g for 30 min followed by centrifugation at 100 000 g for 1 h. Saturated ammonium sulfate solution (200 mL) was added (33% final concentration) and incubated ... phosphorylation of CDK4 on Y17 is mediated by Src family kinases. Results Detection of CDK4 Y17 phosphorylation and kinase activity The N-termini of CDKs 1, 2, 4 and 6 are highly con- served and there is...

Ngày tải lên: 18/02/2014, 18:20

Ngày tải lên: 22/02/2014, 07:20

Ngày tải lên: 29/03/2014, 20:20

Ngày tải lên: 09/08/2014, 03:24

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 09/08/2014, 10:22

Ngày tải lên: 12/08/2014, 04:21

... Generated by DL on the Composite Section. This generator is capable of handling from 1 to 10 spans, and is capable of returning values for continuous sections. This is done by moment distribution. ... span point column 1 = moment column 2 = shear Based on continuous section, constant inertia. 1 1.2 1.4 1.6 1.8 0 500 1000 mc n8 1 n8 sp + disp 0 1 2 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 1 ... of the design lane. Combination 3: For both the negative moment between points of contraflexure under a uniform load on all spans, and reaction at interior piers only. 90% of two design trucks...

Ngày tải lên: 29/09/2013, 12:20

... of tomorrow. WHAT IS PATCH BY EXCEPTION? Patch by Exception is a product feature of KRONE's HIGHBAND 8 and 10 modules that allows the normal cross connect configuration to be wired using ... in configuration is required the new connection can be simply made by plugging a HIGHBAND patch cord into the module to instantly set up the new configuration by overriding the jumpered connection. ... progressive connectivity company is introducing "Patch by Exception", the next generation in cable management systems. Patch by Exception guarantees network managers a system that is not only...

Ngày tải lên: 26/10/2013, 19:15

... corre- sponds to the 40 kDa form, consists of the N-terminal region (N) and one Kunitz domain (K1), whereas HAI-1–NK1LK2, corresponding to the 58 kDa form, consists of the N-terminal region (N), ... separated by SDS ⁄ PAGE under reducing conditions (B) or under nonreducing conditions (C), and analysed by immunoblotting with the anti-c-Myc IgG. The protease domain of TMPRSS13 was quantified by scanning ... tissues producing it. In response to tissue injury, such as hepatic and renal injury, the inactive single-chain HGF is converted to a two-chain form exclusively in the injured tissue. This conversion...

Ngày tải lên: 15/02/2014, 01:20

... followed by Strep-tag II was isolated by BamHI digestion and inserted into the BamHI site of pmGFP–calpain 7. To construct pCMV3xFLAG–IST1 and pGEX–IST1, an IST1 cDNA fragment was amplified by PCR, ... intermolecular reac- tion occur in the autolysis. Therefore, it is premature to draw conclusions regarding the mechanism of the enhancing effects of IST1 on mGFP–calpain 7–Strep autolysis in vitro. Both ... cultured cells. On the other hand, overexpression of FLAG– IST1 DMIM1,2 suppressed the autolysis. This observation might be explained by regarding IST1 as a regulator of the intracellular localization of...

Ngày tải lên: 18/02/2014, 04:20

... all incubation conditions. When rhTLK1 is diluted from high storage concentrations to low assay concentra- tions of 23 ngặmL )1 , which is below the limit for the ATP tetramerization effect, the ... respectively, depending on pre-assay incubation with ATP. Here, the part of ATP that is necessary for tetramerization and how the reaction velocity is influenced by the enzyme concentration are determined. ... scintillation counting. Analysis of kinetic data Kinetic data were fitted by non-linear regression analysis to the MichaelisMenten equation v ẳ V max ẵS=K m ỵẵSị or the Hill equation v ẳ V max ẵS n =K 0:5 n ỵẵS n ị...

Ngày tải lên: 18/02/2014, 12:20

... light on the function of this interaction in the in vivo situation. By influencing the duration of the Ca 2+ signal of ORs in the cilia of the sensory neurons, MUPP1 could have a strong influence on ... interaction was detected using MUPP1 antibodies. Immunohistochemistry Mice were raised and maintained according to governmen- tal and institutional care instructions. Immunohistochemis- try was ... proportion of transiently transfected cells responding to odorant in ratiometric calcium imaging experiments [24–26]. This alteration in the subcellular distribution of MUPP1 upon co-expression with...

Ngày tải lên: 18/02/2014, 13:20

... formation by transferring electrons from NADPH onto oxygen molecules [56]. A defici- ency of one PHOX subunit in NADPH oxidase leads to the inhibition of superoxide generation, and results in chronic ... cytokine synthesis and febrile response induced by SEC1 is dependent on NADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄ FLAP because these phenomena are attenuated by apocynin and not by ETYA, MK ... localization of epitopes on tryptic fragments. Infect Immun 45, 281–285. 5 Cremonesi P, Vimercati C, Pisoni G, Castiglioni B, Luzzana M, Ruffo G & Moroni P (2006) Identification of enterotoxin genes...

Ngày tải lên: 19/02/2014, 00:20

... were isolated as a single peak fraction by a 0.1–1.0 m NaCl linear gradient in R buffer. Histone H1, which exists in the DNA affinity fraction, was separated as a major component stepwise elution ... histone H1 [14], topoisomerase II [15], lamin B1 [16] and SAF-A (hnRNP U) [17]. It is known that the aggregation of DNA is com- patible with the functions of proteins under in vitro conditions. ... and one of the candidates may be linker histone H1 of an aggregative protein. IR provokes ATM-dependent dephosphoryla- tion of histone H1 [42]. A recent study demonstrates that whereas histone...

Ngày tải lên: 19/02/2014, 06:20

... mm Tris ⁄ HCl (pH 7.5). After incubation for 1 h at 4 C, 20 lgặmL )1 of heparin was added and the incubation was continued for 5 min. The complex formation was analysed by electrophoresis on nondenaturing ... protein containing a fused N-terminal histidine tag was purified by binding to and elution from Ni-NTA-affinity matrix [43]. Nuclear extract preparation and fractionation of the B. mori transcription ... mutual contamination. In every fractionation the quantities of fractions had to be optimized because use of larger amounts of any individual fraction tended to result in inhibition of transcription....

Ngày tải lên: 20/02/2014, 02:21

... in transcriptional control and their impact on cellular function. Oncogene 19, 2468–2473. 45. Tone, M., Powell, M.J., Tone, Y., Thompson, S.A. & Waldmann, H. (2000) IL-10 gene expression is controlled ... prothrombinase. This combination of consti- tutive expression and induction may permit a rapid response to inflammation. However, the mechanisms regulating fgl2 expression, either constitutive or ... demonstrated that Fig. 7. EMSA analysis of the function of Ets/Stat3 cis elements. (A) Cross competition of consensus Ets/PEA3, Ets/PU1, and Stat3 cis elements with corresponding transcription...

Ngày tải lên: 20/02/2014, 11:20

... for a region of this construct containing galA and some of the flanking regions, resulting in the genomic sequence of A. niger galA.The presence of one putative intron was confirmed by RT- PCR ... start codon. In addition, one putative PACC site ()549) [31] and two CCAAT sites ()280, )300) were detected. Expression analysis of galA The expression pattern of galA on a selection of mono- saccharides, ... library of A. niger using this fragment as a probe resulted in the isolation of two galA-containing phage k clones. From one of these clones a4.5-kbEcoRI fragment was cloned into pBluescript SK + ,...

Ngày tải lên: 21/02/2014, 01:21

... metal. In all structures solved for the mononuclear SODs, the metal ion is held in place by an absolutely conserved quartet of residues comprising three histidines and one aspartic acid which act as ligands ... no other SODs with this mutation exist naturally. The nature of this phenomenon still remains elusive. Metal specificity may be considered as consisting of two, presumably distinct, stages. First, ... peroxide on the iron-containing superoxide dismutase of Escherichia coli. Biochemistry 26, 1251–1257. 47. Misra, H.P. & Fridovich, I. (1978) Inhibition of superoxide dis- mutase by azide....

Ngày tải lên: 21/02/2014, 01:21

... suggested that LBR binds to a DNA region on chromatin in the RS region. This idea was consistent with a report by Ye & Worman [15], i.e. that a region corresponding to RS binds free DNA. Duband-Goulet ... chromatin is regulated by phosphorylation in the arginine-serine repeat- containing region (RS-region) by multiple kinases. The potential function of LBR and LAP2 in vesicle targeting to chromatin is ... Expression of fusion proteins was induced by the addition of 0.1 m M isopropyl thio-b- D -galactoside, followed by incubation for 6 h at 30 °C. The bacterial cells were collected by centrifugation...

Ngày tải lên: 22/02/2014, 04:20