size exclusion chromatography fieldflow fractionation countercurrent chromatography

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Ngày tải lên: 05/09/2013, 10:15

... reaction (Fig. 1). For this reason, we call this adapted version of diagonal chromatography com- bined fractional diagonal chromatography (COFRAD- IC [32]). It should be clear from the peptide-sorting ... have increasingly become the center of analysis: protein mixtures, either partially purified by prefractionation or as such, are digested with trypsin, and the generated peptide mixture is analyzed. ... referred to as ran- dom sampling or undersampling [19], and it became crucial to add peptide prefractionation before MS analysis. Yates’ group introduced separation of pep- tides based on two...

Ngày tải lên: 18/02/2014, 16:20

... the peptides weredriedinaspeed-vacandkeptat)20 °C until use. Peptide mapping by capillary liquid chromatography microelectrospray tandem MS (LC-lESI-MS/MS) Tryptic peptides were analysed using ... reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS Axel Ducret 1 , Mai Nguyen 2 , David G. Breckenridge 2 and ... (2000) Mol. Cell. Biol. 20, 6731–6740). We describe here the char- acterization by capillary liquid chromatography microelec- trospray tandem MS of a BAP31 immunocomplex isolated from a HepG2 cell...

Ngày tải lên: 20/02/2014, 23:20

... sees Fig. 1.2 1. Rapid analysis, analysis to C8. 80 secs JOURNAL OF CHROMATOGRAPHY LIBRARY-volume 56 chromatography in the petroleum industry edited by E. R. Adlard Burton, ... 8.8.1.4 High temperature gas chromatography 8.9 Conclusions 8.10 Acknowledgments 8.11 References Chapter 9 . Multi-column systems in gas chromatography H . Mahler. T ... particularly for methane, the inevitable major component. Chromatography consists of a series of compromises, including the choice of sample size in order to allow detection of minor components...

Ngày tải lên: 21/02/2014, 01:21

... 969–972. [7] Foucault, A. P., Centrifugal Partition Chromatography, Marcel Dekker, New York 1994. [8] Cao, X. L., High-Speed Countercurrent Chromatography Technology and Application, Chemical ... Book Series- GC Chromatography, http://www.library4science.com). Counter-current chromatography (CCC) including high- speed CCC (HSCCC) a nd high-performance centrifugal partition chromatography ... six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography Six volatile compounds, curdione (1), curcumol (2), germacrone (3), curzerene (4),...

Ngày tải lên: 07/03/2014, 21:20

... component Column Chromatography Gas Liquid Chromatography (GLC) Gas Liquid Chromatography (GLC) High Performance Liquid Chromatography (HPLC) High Performance Liquid Chromatography (HPLC) Paper Chromatography ... Thin Layer Chromatography (TLC) Types of Chromatography Chromatography  There are two basic types of chromatography  Gas  Liquid  Liquid includes TLC and high performance liquid chromatography ... A+B+CABC A+B+CA+B+CABC Column Chromatography  Similar to thin layer chromatography  Stationary phase = silica gel on support  Mobile phase = liquid solvent  In column chromatography, this stationary...

Ngày tải lên: 08/03/2014, 15:35

... phycobilisomes. Eur. J. Biochem. 91, 57–63. 14. Zolla, L. & Bianchetti, M. (2001) High-performance liquid chromatography coupled on-line with electrospray ionization mass spectrometry for the simu ltan ... Timperio, A.M. & Corradini, D. (1997) Rapid resolution by reverse-phase high performance liquid chromatography of the thylakoid membrane proteins of the photosystem II light-harvesting complex. ... and characterization of chloroplast photosystem II antenna of spinach by reversed- phase liquid chromatography. Photosynth. Res. 61, 281–290. 22. Corradini, D., Huber, C.G., Timperio, A.M. &...

Ngày tải lên: 08/03/2014, 16:20

Ngày tải lên: 09/03/2014, 18:55

... Biol Chem 283, 593–602. 31 Tateno H, Nakamura-Tsuruta S & Hirabayashi J (2007) Frontal affinity chromatography: sugar-protein interactions. Nat Protoc 2, 2529–2537. 32 Nakamura S, Yagi F, Totani ... Koyama Y, Suzuki T, Isemura M & Hirabayashi J (2006) Com- parative analysis by frontal affinity chromatography of oligosaccharide specificity of GlcNAc-binding lectins, Griffonia simplicifolia ... K a ), of DC-SIGN, DC-SIGNR and LSECtin. Previously, we developed an automated frontal affin- ity chromatography (FAC) system, which allows high- throughput determination of affinity constants of immobilized...

Ngày tải lên: 15/03/2014, 23:20

... isotopic measurements, Journal of Chromatography A 2011; 1218 (31)5241-5247. Ion Exchange Chromatography - An Overview http://dx.doi.org/10.5772/55652 29 Ion chromatography employing anion-exchange ... non-suppressed ion chromatography. Journal of Chromatogra‐ phy A; 2010; 1217(13) 2070-2073. [28] Moldoveanu S. C. & Kiser M. Gas chromatography/ mass spectrometry versus liquid chromatography/ fluorescence ... phenols with ion chromatography- online electrochemical derivatization based on porous electrode-fluorescence detec‐ tion, Journal of Chromatography A 2012; 1229, 288-292. Column Chromatography 30 •...

Ngày tải lên: 17/03/2014, 02:20

... 2002/657/EC, Journal of Chromatography A 1032 (2004) 23- 29. 6. Che-Yi Lin, Shang-Da Huang - Application of liquid-liquy microextraction and high performance liquid chromatography for the determination ... sulfonamides in environmental water samples using liquid chromatography coupled to electrospray tandem mass spectrometry. Journal of Chromatography A 1157 (2007) 115-121. 8. Guillermina Font. ... spectrometry as an improved methodology for the determination of sulfonamide residues in meat. Journal of Chromatography A 1159 (2007)233-241. xuit thu hoi tren cd lap lai kem vi vay cac mau phan tich...

Ngày tải lên: 20/03/2014, 21:20

... include: ion -exclusion partition chromatography, Donnan exclusion chromatography, and ion- moderated partition chromatography. It has been demonstrated that the retention of Applications of Ion Chromatography ... but differs from other such processes (such as size- exclusion chromatography) in that the suggested mechanism 42 RETENTION PROCESSES IN ION -EXCLUSION CHROMATOGRAPHY: A NEW PERSPECTIVE Where l is ... and aromatic carboxylic acids in ion -exclusion chromatography. J. Chromatogr. A 1999;850:17– 27. 28. Gl ´ od BK. Principles and Applications of ion -exclusion chromatography. Acta Chromato- graphica...

Ngày tải lên: 22/03/2014, 09:20

... sensitive to light and are oxidized or decomposed upon exposure. 6.3 Volumetric Flasks Various sizes. 6.4 Laboratory or Aspirator Vacuum System Sufficient capacity to maintain a minimum vacuum ... A greater vacuum (66 cm [26 in.] of mercury) may be used with disks. 6.5 Micro Syringes Various sizes. 525.2-23 10.3.6.4 Break off a short portion (about 1 m) of the column from the end near the ... surface is modified by chemically bonded octadecyl (C ) groups. The packing must have a narrow size distribution and 18 must not leach organic compounds into the eluting solvent. One liter of...

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