sequence structure and function analysis

... Location Biotics and abiotics Energy and material flow Human impacts, consequences and solutions Conclusion LOCATION  Thong Nhat Park  Peninsula : 1200 m2  Including : mainland, lake, plants, ... restaurant in this area and gases from vehicles on Le Duan road HUMAN IMPACTS - CONSEQUENCES SOLUTIONS  Increasing the number of wastebasket  Planning the rubbish dumps and quick transporting ... the restaurant and visitors to keep the park green, clean and beautiful CONCLUSIONS  Plant species on peninsula are quite diverse  People are having huge impacts on the formation and development...

Ngày tải lên: 28/05/2016, 09:28

... focus primarily on the actual chromatin structure of active genes, with regard to nucleosomal organization and higher order structure, and the chromatin structure changes that occur during locus ... Deoxyribonuclease I sensitivity of the nontranscribed sequences flanking the 5¢ and 3¢ ends of the ovomucoid gene and the ovalbumin and its related X and Y genes in hen oviduct nuclei Biochemistry 19, ... Chromatin structure of transcriptionally competent and repressed genes EMBO J 9, 3997–4006 97 Woodcock CL, Skoultchi AI & Fan Y (2006) Role of linker histone in chromatin structure and function: ...

Ngày tải lên: 14/02/2014, 18:20

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned into ... clarified the structural and functional importance of the v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neurons and cerebellar granule ... polymerization, stability and rearrangement of microtubules in neuronal dendrites [13–15] and is associated with some neurological and psychiatric disorders [16,17] However, the structure function relationship...

Ngày tải lên: 14/02/2014, 19:20

... marine and freshwater ecosystems James H Brown, Andrew P Allen and James F Gillooly Body size and suspension feeding Stuart Humphries 16 Life histories and body size 10 David Atkinson and Andrew ... taxonomic and functional groups of organisms, and types of ecosystems Here we apply the metabolic theory of ecology to focus on some important correlates and consequences of body size in marine and ... empirical and theoretical work in biological oceanography and limnology that relates the structure, function and biotic composition of these systems to the body sizes of the organisms present and the...

Ngày tải lên: 17/02/2014, 19:20

... bioinformatic analysis of the Arabidopsis genome sequence revealed more than 50 AP-like proteins of this family [86,87] In a first analysis based on their gene structure organization and evolutionary ... APs References Davies, D.R (1990) The structure and function of the aspartic proteinases Annu Rev Biophys Biophys Chem 19, 189–215 Dunn, B.M (2002) Structure and mechanism of the pepsin-like family ... intracellular, and subcellular localization studies revealed that they accumulate essentially inside protein storage vacuoles Biochemical and immunocytochemistry analysis of barley roots and leaves...

Ngày tải lên: 19/02/2014, 12:20

... a5 and/ or b3 subunit [12] The a5 and b3 subunits are very similar in sequence and both appear Neuronal nAChRs are present throughout the central and peripheral nervous system, at both pre- and ... investigation and understanding of their structure- activity relationships, may start to provide a rational way to develop additional pharmacological tools for the elucidation of nAChR structure and function ... similar sequences and selectivities for recombinant receptors could be represented by PIA and MII (SNPV motif in the first loop and nanomolar activity on a3/a6 containing nAChRs) and EpI and ImI...

Ngày tải lên: 19/02/2014, 12:20

... Graether and B D Sykes (Eur J Biochem 271) Fig Fish AFP structures and model The structures of the fish AFPs are shown as ribbon diagrams with coil structure shown as yellow, a-helices as red and b-strands ... [31,32,36] and their threedimensional structures were determined [34,37–40] In subsequent sections, we describe the structure and dynamics of each protein, and present a comparison of sbwAFP and TmAFP ... other and with proteins that have a similar fold Structure of sbwAFP and TmAFP The structure of sbwAFP has been determined by X-ray ˚ crystallography to 2.5 A and by NMR at both 30 °C and °C...

Ngày tải lên: 19/02/2014, 16:20

... three antiparallel strands (1, and 7) and strand 6, which is parallel to strand b-Strands are located between the four a-helices (a1, 278–294; a2, 305–313; a4, 374–381; and a5, 397–405), with ... elements: b-strands (b3, 329–335; b4, 339–344; and b5, 367–372) and a distorted a-helix (a3, 348–356) (Fig 3B,C) The three b-strands of the minidomain are all antiparallel, and form a single b -structure ... the structure of the protein core (residues 277–328 and 373–413) in solution and in the solid state, and indicates their similarity A superposition of the C-domain NMR structure on the crystal structure...

Ngày tải lên: 06/03/2014, 11:20

... between TLP and transthyretin indicate conservation of the TLP ⁄ TTR structure through evolution Motifs identified in (A) TLP sequences and (B) transthyretin+TLP sequences (A) In the set of TLP sequences, ... between strands E and F in transthyretin The two transthyretin dimers associate, via nonpolar interactions, between the loops joining stands G and H with the loops joining strands A and B, making ... between the structures of TLP and transthyretins are found in the loop connecting b-strands B and C, which is highly exposed to the solvent in TLP [17] Interruptions in the b-strands A, G and H are...

Ngày tải lên: 07/03/2014, 00:20

... antiparallel b-strands is abutted by three a-helices (a1, a2, and a¢) The b-sheet in type I KH domains consists of three b-strands in the order b1, b¢ and b2 The b1-strand and b2-strand are parallel ... the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adjacent and antiparallel to the b1-strand (Fig 1) The length and sequence of the variable loop are different ... recognition sequence, and a Structure and function of KH domains Fig Crystal structure of Nova-2 KH3 bound to SELEX RNA The third KH domain of the protein Nova-2 binds to the tetranucleotide sequence...

Ngày tải lên: 07/03/2014, 05:20

... Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur J Biochem 269) 4213 Fig De novo and salvage pathway of UDP-GlcNAc biosynthesis EXPERIMENTAL ... chromatography and detection by a specific antibody using Western-blot analysis GlcNAc kinase cDNA encodes for a protein with 39 kDa, the His-tag for a kDa protein part The analysis of wild-type and mutated ... Overall sequence similarities were investigated using the software [16] and the NR-DATABASE Protein sequences were aligned by using the MULTALIN software [17] The algorithms used for secondary structure...

Ngày tải lên: 08/03/2014, 10:20

... s/Lausanne (Switzerland) gss Genome Survey Sequence, includes single-pass genomic data, exon-trapped sequences, and Alu PCR sequences htgs High Throughput Genomic Sequences Nucleic Acid Sequence What ... double-stranded DNA λ phage 48,500 Linear double-stranded DNA T7 phage 40,000 Linear double-stranded DNA M13 phage 6,400 Closed-circular single-stranded DNA MS2 phage 3,600 Linear single-stranded ... amino acid query sequence against a protein sequence database blastn Compares a nucleotide query sequence against a nucleotide sequence database blastx Compares a nucleotide query sequence translated...

Ngày tải lên: 13/03/2014, 16:42

... larger:   “Frilly” edged…… Long and narrow…  Round cells will always be small Prokaryotic Cell Structure  Prokaryotic Cells are smaller and simpler in structure than eukaryotic cells   ... cells    why are cells so small? Prokaryotic cells Eukaryotic cells Cell size and shape –    Organelles and structure in all eukaryotic cell Organelles in plant cells but not animal Cell ... Membrane bound organelles Prokaryotic Cell Structure  Structures       Plasma membrane Cell wall Cytoplasm with ribosomes Nucleoid Capsule* Flagella* and pili* *present in some, but not all...

Ngày tải lên: 13/03/2014, 19:31

... RER Functions of Eukaryotic Cell Features Structure Function( s) Golgi Apparatus Collects, modifies, packages and distributes proteins and lipids from ER Functions of Eukaryotic Cell Features Structure ... cell? Functions of Eukaryotic Cell Features Structure Function( s) Regulates passage of Plasma materials into and out of cell Membrane Cell-Cell Recognition Functions of Eukaryotic Cell Features Structure ... Plasma Membrane Functions of Eukaryotic Cell Features Structure Peroxisome Function Removes harmful oxidants from cells Functions of Eukaryotic Cell Features Structure Mitochondrion Function Captures...

Ngày tải lên: 13/03/2014, 19:31

... buffer, pH 7.0, and its UV spectra were recorded between 250 and 190 nm Molecular modelling Structure manipulations and analysis were performed using the PYMOL software [41] Results and Discussion ... active enzyme intermediate, and kfast and kslow are Affinity labelling of maize GST I (Eur J Biochem 271) 3505 the rate constants for the slow and fast phase of the reaction Analysis was performed using ... system, and by HPLC on a C18 reverse-phase column It was found to be at least 98.4% pure Fig Structure of SDTG (A) and time course of inactivation of recombinant GST I by SDTG at pH 6.5 and 25...

Ngày tải lên: 16/03/2014, 16:20

... Education, Science, and Culture of Japan, and a fellowship from Katayama Chemical Industries, Co., Ltd (Osaka) to T.Y Ó FEBS 2004 Effects of DiS-C on mitochondrial structure and function (Eur J Biochem ... interpretation, we further characterized the actions of DiS-C3(5) on the mitochondrial structure and function and compared them with those of Ca2+, known as a typical PT inducer Like that of Ca2+, ... in the legend in Fig and subjected to TEM analysis (A) The appearance of nontreated control mitochondria (B) and (C) The appearances of mitochondria treated with 100 lM Ca2+ and 50 lM DiS-C3(5),...

Ngày tải lên: 16/03/2014, 18:20

... physiological function( s) 5650 Results and Discussion Modelling of the ab ‘monomers’ of HPT1 and HPT2 Figure shows the sequence alignment of the two HPT variants and C1R C1R displays 29% sequence ... C1R (PDB code: 1GPZ [27]) and of the modelled structures of HPT1 and HPT2 HPT1 residue Cys15 and HPT2 residues Cys15 and Cys74 are shown in spacefill representation This and the following figures ... covering both the HPT1 a- and b-chains) and HPT2 (spanning residues 15–387, covering both HPT2 a- and b-chains) variants The homology is widespread along all the sequences, and almost all the Cys...

Ngày tải lên: 23/03/2014, 06:20

... Genomic structure and expression analysis of the Cc RNase Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 ... Genomic structure and expression analysis of the Cc RNase A B FEBS Journal 275 (2008) 6217–6227 ª 2008 The Authors Journal compilation ª 2008 FEBS 6223 Genomic structure and expression analysis ... 6225 Genomic structure and expression analysis of the Cc RNase Expression and purification of Cc RNase recombinant protein The ORF of the Cc RNase cDNA 02 sequence was amplified by PCR and then sub-cloned...

Ngày tải lên: 23/03/2014, 06:20

... diabetic human placenta and then to purify and examine kinetic properties The fresh placenta was obtained and the tissues were cooled, washed and perfused and weighed, then minced and suspended in three ... specific functions related to virulence and calcium binding We have collected several NMR data sets in order to study the secondary and tertiary structure of CBP Importantly, we will present the structure ... specific ligands Most of these transcription regulators bind cognate DNA in the presence or absence of ligand, typically repressing in the absence of ligand and activating when ligand has bound...

Ngày tải lên: 23/03/2014, 15:20

... were used The expression of PDGF-R and c-Kit, their ligands, MMP-9 and signal transduction pathways PI3/Akt and MAPK were studied by RT-PCT and western blotting and immunocytochemistry The three ... surface decreased, and cells tendency to form gland and intercellular conjunction structures also be showed, and cellular apparatus were abundant in plasma maturely, nuclei, endoreclure and mitochondria ... synthes´ ized by Trezl and co-workers from CH2O and L-arginine may belong to that type of molecules HMA was found in human blood and urine, and in plants (leaves, fruits and vegetables) in higher...

Ngày tải lên: 23/03/2014, 15:20