secretors and nonsecretors body fluids

... (1993), Harley (1995), Hatch and Brown (1995), Coady and Huckin (1997), Schmitt and Mc Carthy (1997), Atkins (1998), Wesche and Paribakht (1999), Read (2000), Schmitt (2000), and Nation (2001) These ... et al (1978), Bialystok (1979), Cohen and Aphek (1981), Wenden (1982), Chamot and O'Malley (1987), Politzer and Mc Groarty (1985), Conti and Kolsody (1997), and many others studied strategies used ... knowledge), the learning task at hand (e.g., type, complexity, difficulty, and generality), and the learning environment (e.g., the learning culture, the richness of input and output opportunities) Oxford...

Ngày tải lên: 07/11/2012, 14:54

... of extra tasks, material and activities and be helpful, enthusiastic and humorous as well Hopefully, the thesis can be considered a small contribution to the teaching and learning reading at the ... reading and reading activities Chapter III,:, The study, provides the setting of the study, subjects, instruments and data analysis process Chapter IV:, Data analysis and discussion, analyses and ... consultation and input of learners and that not presuppose objectives in advance - techniques that enhance a learner’s sense of competence and self-worth - techniques that allow for learner creativity and...

Ngày tải lên: 07/09/2013, 13:48

... decorins and decorin cores, respectively Left lane: standard protein markers and their molecular masses (in kDa) The core protein is present as two bands with apparent molecular masses of 47 and 42 ... tendon decorin (lanes and 2) and recombinant decorin (lanes 3–4) we have used in this work, both before (lanes 1,3) and after (lanes 2,4) chondroitinase ABC digestion About 10 lg and lg were analyzed ... type I collagen molecules, and the presence of keratan sulfate PGs at the a and c bands in the overlap zone [23,24] A study using isolated type I procollagen molecules and decorin extracted from...

Ngày tải lên: 21/02/2014, 15:20

... h at room temperature, and then reacted with various antisera and antibody Antisera against annexin A1, annexin A2 and annexin A5 were raised in both rabbit and mouse, and antiserum against dicalcin ... mM Ca2+, and the 1st and the 10th extracts were subjected to SDS ⁄ PAGE (high-Ca2+ wash and high-Ca2+ wash 10) The beads were finally washed with K-gluc buffer supplemented with mM EGTA, and the ... annexin A2 and annexin A5 by western blot analysis (A) Determination that the 38 kDa proteins are a mixture of annexin A1 and A2 and that the 35 kDa proteins are a mixture of annexin A5 and proteolytic...

Ngày tải lên: 07/03/2014, 05:20

... gel migration of GC-rich random-template (lanes and 2), ATT-template (lanes and 4), AAT-template (lanes and 6) and : mixture of ATT- and AATtemplate Samples with (+) and without (–) DAPI are indicated ... at a : drug/template molar ratio was about 12 and 15 nm, and the increase in the fluorescence intensity was only 2.2- and 1.6-fold for AAT- and random-template, respectively (Fig 4) UV thermal ... structural effects on singlestranded DNA and RNA The main genetic processes such as replication, transcription and translation involve individual strands of DNA and RNA, emphasizing the importance...

Ngày tải lên: 16/03/2014, 23:20

... antibody; N Standart (University of Cambridge, UK) for providing the a-XStaufen antibody; K Czaplinski and I W Mattaj (EMBL, Heidelberg, Germany) for providing the a-40LoVe antibody; and Andreas ... injection, and analyzed the nuclear and cytoplasmic fractions by SDS ⁄ PAGE and phosphoimaging (Fig 6B) Although a minor fraction of 42Sp50 is exported from the nucleus after and h (lanes 12 and 14), ... 5¢-GGAATTCCATGAGTGAGGC GGAACC-3¢ and FRGY-2B_R: 5¢-GGTCTAGACAGCG ACTGAGTTCATTCTG-3¢ and ligated into the EcoRI and XbaI sites of pCS2+-MT Oocyte microinjection and cultivation Oocytes were obtained...

Ngày tải lên: 23/03/2014, 03:20

... subdomains designated a, b and c, and that the protein has two sugar-binding sites in subdomains a and c [24,25] Therefore, determination of the sugar-binding ability and specificity for each of ... titration curves measured for the main chain and amide proton groups of Ile102, Ile104, Cys115, Trp117, Lys118 and Gly122, and the side chain nitrogen and amide proton group of Asn124 in the c sugarbinding ... amide and amide proton of Asp18, and side chain amide and amide proton (marked by ‘sc’) of Trp33 in EW29Ch during the titration of lactose (A, top), b-Me-Gal (A, bottom), melibiose (B, top) and...

Ngày tải lên: 23/03/2014, 04:21

... and bipyridyl on optical spectra of hemin and membrane bound flavocytochrome b (A) Traces a1 and a2: absolute spectra of oxidized and dithionite-reduced hemin (10 lM), respectively Traces b1 and ... benzylimidazole and trace c to flavocytochrome b treated with 50 mM benzylimidazole Traces d and e correspond to hemin (1 mM) in DMF untreated and treated with 50 mM benzylimidazole, respectively (A) and ... and INT (respectively 10 and nmol) INT were also significantly diminished, compared to the control (trace a) In summary, using the flavin analog 5-deaza FAD and the heme inhibitors, bipyridyl and...

Ngày tải lên: 31/03/2014, 21:21

... that contribute to the stable activation of RASFs, the precise cause and nature of this activation, as well as its relevance and consequences, are matters of debate The present data indicate very ... back to IL-16, a cytokine that has been demonstrated at elevated levels in the sera [15,16] and synovial fluids [17] of patients with RA, but that has not been studied extensively in RA due to controversial ... present research on the interaction of RA-IgG and RASFs as well as other recent data, however, may change the picture It has been reported by Huizinga and colleagues that in a cohort of patients...

Ngày tải lên: 09/08/2014, 06:22

... flowers and fruits are rich in flavonoids where they act as pollinator attractants and seed dispersers, UV-scavengers and are involved in disease resistance [13] In red grape, flavanols and anthocyanins ... of genes linked to auxins and ethylene and fewer genes involved in the abscisic acid, brassinosteroid and gibberellic acid pathways as previously reported [36-38], and as showed in Table of [21] ... experiment: their microarray ID, VVGI5 ID and its annotation, unique oligo sequence, M and A values, p-value and Bonferroni-adjusted p-value, t-statistic and B-statistic Click here for file [http://www.biomedcentral.com/content/supplementary/14712229-9-104-S1.txt]...

Ngày tải lên: 12/08/2014, 03:21

... isolate and examine the issues of interest (here, the intrinsic dynamics of the protein, water dynamics and DNA conformation) relatively unfettered and unclouded by the dynamics driving unfolding and ... intercalating, interfacial and bulk waters in the cognate and noncognate complexes 83 xi Table 5-1 Mean distance (± standard error) of the phosphorus atom in the cleavage site and the aminoacid ... correlation function for interfacial (A and B) and intercalating waters (C and D) 72 Figure 4-4 Mean-squared displacement of water molecules in the interfacial and intercalating regions ...

Ngày tải lên: 09/09/2015, 18:49

... ligands can also be recognized The PDZ domain is composed of six  strands (A-F) and two  helices (A and B) The ligand binding groove is structurally formed by a connecting loop between A and ... cue and is formed upon nectin and cadherin transassociated clustering and throughout its maturation, sequentially recruits adherens junction and tight junction components, including catenins and ... loss and tumorigenesis remained uncertain However the discovery of the Scrib tumor suppressor and cell polarity complex has bridged this and allowed a clearer understanding of cell polarity and...

Ngày tải lên: 14/09/2015, 08:24

... was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and 5) for h at 37 °C and then subjected to partial digestion with trypsin ... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the ... in lanes and with those in lanes 3, and reveals that the FEBS Journal 273 (2006) 199–207 ª 2005 The Authors Journal compilation ª 2005 FEBS 203 Disassembly and recruitment of hRad51 and hRad52...

Ngày tải lên: 16/03/2014, 14:20

... and Methods 2.1 Construction of expression plasmids EGFP and GST were PCR amplified from pEGFP (Clontech, USA) and pGEX-4T1 (Pharmacia Biotech, USA) respectively, and cloned into the pTWIN1 and ... both in vitro and in vivo, and its applications in different areas related to proteomics and chemical biology, namely protein microarray technologies for large-scale protein analysis and live cell ... Sap I and Pst I sites of the pTWIN vector The target gene sequence is in lower case letters Following digestion of the vector and PCR products by endonucleases Sap I and Pst I, the insert and vector...

Ngày tải lên: 08/11/2015, 16:30

... Maintenance and growth of mammalian and yeast cells Genotypes and sources of S pombe strains, mammalian cell lines and plasmids used in this study are summarized in Table CD4-positive H9 and CEM-SS ... life cycle and pathogenesis For example, Vpr is required both in vitro and in vivo for viral pathogenesis and efficient viral infection of non-dividing host cells such as monocytes and macrophages ... pombe) through a pathway involving regulatory and catalytic subunits of PP2A and acting on both Wee1 and Cdc25 Virology 2001, 287:359-370 Albano CR, Randers-Eichhorn L, Bentley WE, Rao G: Green...

Ngày tải lên: 13/08/2014, 09:20

... S-(2-boronoethyl)-l-cysteine (BEC) His120 and His145, and the corresponding His101 and His126 in arginase I, were described among the ligands for coordination of Mn2+A and Mn2+B, respectively [9,24] However, ... arginase II and agmatinase, as both enzymes are mitochondrial and functionally different [22] A key factor is the a-carboxyl group of the substrate, which makes the difference between arginine and agmatine ... of human arginase II with substrate and metal ions was examined by sitedirected mutagenesis and kinetic studies Selection of target residues (Asp149, His120 and His145) was based on the roles...

Ngày tải lên: 20/02/2014, 02:21

... Actin and small heat shock protein (Eur J Biochem 270) 893 conditions G-actin (10–15 lM) was cooled to 25 °C, and aggregation was initiated by the addition of KCl and MgCl2 up to 50 mM and mM, ... measured according to Pollard [25] and Miron et al [12] Briefly, polymerization was performed in buffer G and was initiated by the addition of KCl and MgCl2 up to 50 mM and mM, respectively Different ... environment and affect parameter A Preparations of actin with A > 2.56 contain less than 2% of inactivated actin, whereas parameter A for inactivated and completely unfolded actin is equal to 1.3 and...

Ngày tải lên: 20/02/2014, 23:20

... antigen–antibody complexes were collected, and after rigorous washing in nonionic detergent-containing buffer and elution of antibodybound protein, the samples were separated by SDS ⁄ PAGE and analyzed ... exponential growth phase, and the cells were then washed and resuspended in SMM without nitrate, and finally incubated for a further 20 Samples were taken before and after the shift, and soluble cell-free ... and GS, in particular, as potential partner proteins of TnrA To this end, immunoprecipitation assays were performed with cellfree extracts from both mutant and wild-type nitrategrown cells, and...

Ngày tải lên: 06/03/2014, 00:21

... SMEZ1 and 2), three have one Cys (SEI, SEK and SPEC), two have three Cys (SEG and SPEA) and only SSA has more than that, five Cys As discussed later, the fact that SSA has two Cys residues (Cys26 and ... hVb2.1hCb2 and hVb1hCb2 (genes kindly provided by U Utz and R P Sekaly, University of Montreal, Canada), were cloned between the NdeI and EcoRI restriction sites of the pET17b expression vector and ... NaCl/Pi and concentrated to mgÆmL)1 hVb2.1hCb2 and hVb1hCb2 were also produced as inclusion bodies and refolded at pH 8.5 Purification steps included gel filtration on a Superdex 200 FPLC column and...

Ngày tải lên: 07/03/2014, 16:20