rna mapping protocols northern blot and amplification of cdna ends

... °C in standard buffer: 50 mM Tris, 150 mM NaCl, pH 7.5 MS2 bacteriophage and mutant samples Fig Structure of the whole capsid of bacteriophage MS2 and of the coat protein dimer bound to RNA (A) ... emitted at wavenumber mi and the summation is carried out over the range of appreciable values of F The values of center of spectral mass of tryptophan were converted into degree of denaturation/dissociation ... fraction Fig Dissociation and denaturation of wild-type (WT) and mutant MS2 particles (A) Light scattering and spectral center of mass measurements of WT MS2 as a function of urea concentration To...

Ngày tải lên: 19/02/2014, 12:20

... cis-prenyltransferases, was termed LT600 3¢- and 5¢-RACE reaction and cloning of Hevea cis-prenyltransferase cDNA Cloning of the full-length cDNA of Hevea cis-prenyltransferase, used poly(A)+ RNA from latex as the ... poly(A) tail of 15 bp (Fig 1A) The cDNA of HRT2 was 1051 bp in length containing an 852 bp ORF (Fig 1B) and with 92% sequence homolgy to that of HRT1 The complete cDNA sequences of HRT1 and HRT2 ... both probes gave specific hybridization bands of kb which are consistent with the sizes of each of the cDNAs The expression patterns of HRT1 and HRT2 mRNAs among the examined Hevea tissues seem...

Ngày tải lên: 07/03/2014, 21:20

... the northern border of Maine The study areas include portions of the St John River Valley near Fort Kent and another section of the valley stretching from the town of Grande Isle to the town of ... silt, clay, and fine sand, but may include coarse sand, gravel and cobbles Alluvium underlies the flood plains and terraces of the St John River A total of 14.3 km2 or 6.3 percent of the areas ... deposits are 7.5 percent of the study areas or approximately 17 km2 Swamp and marsh deposits are comprised of peat, silt, and sand, and formed by the accumulation of sediments and organic material...

Ngày tải lên: 08/03/2014, 23:20

... nt of the 3¢-end of the HCV minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synthesis [17] Oh et al [18] showed that RNA synthesis from the HCV minus-strand RNA ... 3877 Binding and replication of 3¢-end of HCV minus RNA A T Astier-Gin et al B Fig RNA synthesized by NS5B in the presence of wild-type and mutated RNAs Wild-type and mutated (–)IRES RNAs were used ... using new mutants of the 3¢-end of minus-strand RNA Results Effect of mutations in the 3¢- or the 5¢-end of the (–)IRES HCV RNA template on RNA synthesis The secondary structure of the 3¢-terminal...

Ngày tải lên: 20/02/2014, 01:20

... [25,26] We exploited this specific feature of the hairpin ribozyme in a scheme of sitedirected and patchwise exchange of RNA sequences [16,17] Combination of two hairpin ribozymes into one molecule ... structure of the reverse-joined ribozyme unit for twin ribozyme design consists of two domains joined by a single-stranded linker of eight adenosine residues and binds the 3¢-terminal part of its ... Addition of the fragment S20 cp led to the formation of new products detected as three additional bands (lanes and 4) These signals correspond to the 29- and 35-mer resulting from ligation of the...

Ngày tải lên: 20/02/2014, 01:20

... unprocessed pri-miRNAs However, expression of the marker (regardless of the placement location) could only occur at the expense of failure of cleavage of primiRNA by Drosha [8,9] In contrast and in summary, ... insertion of artificial miRNAs Two steps of cloning were used to construct vectors expressing miR30- and miR155-based artificial miRNAs, pSM30 and pSM155 Part of the cytomegalovirus (CMV) promoter and ... production of siRNAs and the expression of EGFP, because the RNA precursors for both can be processed better or stabilized in the nuclei 5422 FEBS Journal 273 (2006) 5421–5427 ª 2006 The Authors Journal...

Ngày tải lên: 07/03/2014, 11:20

... (control of negative externalities) • Level and development of external relations are clear indicators of success The degree of external orientation of the city is indicated by external links ... intangible and often non-monetary Advertising and promotion is only one of many possible sets of marketing measures The choice of measures depends on the choice of marketing strategies and goals of the ... research, selection of the cases, collection of the empirical data, analysis and interpretation of the data and discusses the quality of the data and validity of the study Chapter consists of the four...

Ngày tải lên: 23/03/2014, 04:21

... the left and right of the sequences show the coordinate of nucleotides and amino acids in corresponding lines The full-length cDNA encoding the putative GIH of P monodon was composed of 861 nucleotides ... and muscle (M) using the specific primers for the 5¢-region of Pem-GIH The negative PCR is indicated by )ve The actin transcript of P monodon was used as an internal control of the amount of RNA ... GIH-dsRNA (B) Expression of Pem-GIH in the abdominal nerve cord of adult female shrimp at and h after incubating without ()) or with (+) GIH-dsRNA or GFP-dsRNA as indicated (C) Expression of Pem-GIH...

Ngày tải lên: 23/03/2014, 07:20

... 5671 Functional analysis of CHH by RNAi Glucose (mg/dL) 20 16 J M Lugo et al 0h 24 h 12 Saline Unrelated dsRNA CHH dsRNA Fig Effects of injection of dsRNA in the profile of the hemolymph glucose ... means of mortar and pestle for RNA extraction RNA isolation Total RNA from different shrimp tissues was extracted using RNAgents Total RNA Isolation System (Promega, Madison, WI, USA) and was ... to the instructions of the manufacturer Northern blot analysis Northern blot analysis was used to characterize the expression of the shrimp CHH gene Ten micrograms of each RNA sample were separated...

Ngày tải lên: 23/03/2014, 10:20

... chromosome at 6p21.3 and mouse chromosome In a Northern blot and hybridization of total RNA from adult zebrafish to a [a32P]dATP-labelled zebrafish FABP3 cDNA, we detected a broad band of RNA of  780 nucleotides ... major band of approximately 200 bp, and two minor bands of 250 bp and 180 bp, were observed after agarose gel electrophoresis of nested PCR products from the CIP/TAPtreated RNA No specific band was ... Cloning of the zebrafish FABP3 cDNA by 3¢ RACE Agarose gel electrophoretic separation of 3¢ RACE products for zebrafish FABP3 cDNA cloning Both the major band of  650 bp and the minor band of  570...

Ngày tải lên: 31/03/2014, 07:20

... father HIS maternal haplotype carries allele of Dl and allele of Ml Mr and Mrs Smith have four children, two boys and two girls One of the boys and one of the girls has red hair Both of the red-haired ... the paternal and maternal identification for the father of this pedigree is and 7, respectively, but the mother’s father and mother not appear m this database, so her paternal and maternal identifications ... “x” for unknown The paternal and maternal identifications of the three children are and The grandparents’ parents not appear m the pedigree, so their paternal and maternal identifications are...

Ngày tải lên: 11/04/2014, 09:42

... infection of cells, the RNPs are released into the cytoplasm and subsequently enter the nucleus where replication of the vRNA results in the production of both mRNA and complementary RNA (cRNA), ... Journal 2007, 4:102 Background The type A and B influenza viruses have genomes consisting of eight negative-sense single-stranded viral RNAs (vRNAs), each of which contains a coding region and ... a canine RNA pol I promoter derived from MDCK cells as a refinement of the human RNA pol I system developed by Hoffmann and co-workers [3] Results and discussion Cloning the canine RNA polymerase...

Ngày tải lên: 18/06/2014, 18:20

... awareness, lack of familiarity with the protocol, lack of agreement, lack of demonstrated safety and efficacy, lack of known improved outcome, lack of ability to overcome the inertia of previous ... clinical data from the charts on diagnosis and use of sedatives and hemodynamic treatments The mismatch between human ability and the vast amount of data and information contributes to variation ... (monitors and/ or ventilators) and test ECP robustness and reliability, and industry to finalize a product that will receive a European Community marking (CE mark) and a U.S marking (Food and Drug...

Ngày tải lên: 18/06/2014, 18:20

... [39-41] A team of 15 data collectors was recruited for the survey (8 men and women) who were all from the Acholi region of northern Uganda, spoke fluent Luo and English, and had experience of data collection ... in Northern Uganda J Epidemiol Community Health 2008 Bowden A, Fox-Rushby JA: A systematic and critical review of the process of translation and adaptation of generic healthrelated quality of ... Health and Quality of Life Outcomes 2008, 6:108 health of conflict-affected populations This study provides evidence on the reliability and validity of the SF-8 amongst IDPs in northern Uganda...

Ngày tải lên: 18/06/2014, 19:20

... infection of cells, the RNPs are released into the cytoplasm and subsequently enter the nucleus where replication of the vRNA results in the production of both mRNA and complementary RNA (cRNA), ... Journal 2007, 4:102 Background The type A and B influenza viruses have genomes consisting of eight negative-sense single-stranded viral RNAs (vRNAs), each of which contains a coding region and ... a canine RNA pol I promoter derived from MDCK cells as a refinement of the human RNA pol I system developed by Hoffmann and co-workers [3] Results and discussion Cloning the canine RNA polymerase...

Ngày tải lên: 20/06/2014, 01:20

... refrigerator, sofa, cupboard, mobile phone); (4) building material (floor and walls); (5) number of chickens, goats, pigs and cows owned; and (6) use of land for Falnes et al Journal of the International ... model: (1) the number of rooms and beds in the household and the number of people living in the household per room and per bed; (2) type of toilet, source of fuel for light and cooking; (3) assets ... Quantification of the utilization of the PMTCT service in terms of numbers of mothers counselled and tested Attitudes to the And PMTCT programme Experiences of the Insight into experiences and attitudes...

Ngày tải lên: 20/06/2014, 08:20

... and there is time and time and there is time and Status of harvest handling for vegetables in Nghe An province Most agro-production in rural areas of Vietnam is from small farmer households and ... after the implantation of the project, awareness of the farmers about use of new varieties have been improved, and many have used improved varieties Seed sources and methods of propagation have ... varieties and methods of pest and disease prevention by use of IPM and use more bio-pesticides Since the 021/06VIE project commenced in 2007, vegetable growers have been practicing and undertaken...

Ngày tải lên: 21/06/2014, 04:20

... maximizing yield and quality of the produce they grow However, there are several aspects of the vegetable industry in Vietnam which currently limit expansion and development of the industry and financial ... will be run by ASINCV and RIFAV, and coordinated by Nguyen Quang Hao and Phan Van Vinh of the Extension and Technology Transfer Department at ASINCV Postharvest Research and Training: Postharvest ... Australia and have undertaken intensive training in crop production and quality assurance management and most importantly the first trial of watermelons was grown, harvested and sold to Metro Cash and...

Ngày tải lên: 21/06/2014, 04:20

... and postharvest handling of cabbage Field research skills of staff at ASINCV have been enhanced through the successful planning, execution and reporting of field trials Skills and knowledge of ... delivered to farmers in Quynh Luong and Hung Ding communes during this period on the harvesting and handling of cabbage as well as the production of watermelon (ix) Trainer of Trainer workshops were presented ... 50 tonnes of cabbages were delivered in good condition and sold through the Metro cash and Carry network of stores, primarily in the north (iv) Vinh – Nghe Ahn Brand has been developed and this...

Ngày tải lên: 21/06/2014, 04:20

... yield, quality and marketing of vegetable crops in Northern Central Vietnam through improved varieties, GAP principles and farmer focused training Agricultural Science Institute for Northern Central ... recognition of region and season provides an good foundation for effective market definition and effective supply of these markets Attachments Supply chain review Crop specifications for watermelon and ... Vietnam, and also a strong recognition among consumers of seasonality and the regions in which produce is grown This is quite different from Australia for example, where the expectation is more often...

Ngày tải lên: 21/06/2014, 04:20