reprogramming of dpsc to induced pluripotent stem cells

... approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse embryonic ... the delivery into somatic cells of a large number of genes (six reprogramming factors in addition to SV40LT and EBNA1) in multiple vectors (two to three), with low reprogramming efficiency (around ... desirable is to avoid genetic material and manipulation altogether Indeed, the number of reprogramming factors required to generate iPSCs has been reduced [20,21], and neural stem cells have recently...

Ngày tải lên: 14/08/2014, 21:20

... lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of histone H3; MacroH2A, histone ... the cell type of origin used to derive iPSC (c) H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone...

Ngày tải lên: 11/08/2014, 12:21

... restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... ……………10 Figure Reprogramming of somatic cells with transcription factors…………… 14 Figure Stages of reprogramming to mouse iPSCs………………………… …….20 Figure Mechanism of action of nuclear receptors…………………………………40...

Ngày tải lên: 09/09/2015, 18:56

... of cells transitioning from fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent ... transfection 42 4.7 Packaging of Lentiviruses for the induction of Pluripotent Stem Cells ……………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells …………………………………………………………………… ... differentiation of Human IPS cell lines 27 3.5 Generation of Porcine Induced Pluripotent Stem Cells 29 3.5.1 Factors used for reprogramming of porcine cells 29 3.5.2 Reprogramming techniques of porcine...

Ngày tải lên: 30/09/2015, 10:13

... the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki T, Saido TC, Ito Y, Yokofujita J, Okeda ... and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... Parkinson’s disease patientderived induced pluripotent stem cells free of viral reprogramming factors Cell 2009, 136:964-977 68 Braak H, Sastre M, Del Tredici K: Development of α-synuclein immunoreactive...

Ngày tải lên: 11/08/2014, 12:21

... representative of three independent experiments Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2 -induced oxidative stress 251 Fig Effect of hydrogen peroxide (H2O2) -induced ... activation of NF-κB [44] Therefore, we suggest that the Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2 -induced oxidative stress 253 Fig Effect of dihydrotestosterone ... Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2 -induced oxidative stress 255 10, 423-426 12 Brook FA, Gardner RL The origin and efficient derivation of embryonic stem cells...

Ngày tải lên: 07/08/2014, 20:23

... transplantation of stem cells, it is very important to observe the survival and curative effects of transplanted stem cells Traditional immunohistochemical method can easily identify the transplanted cells ... mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud N, et al Mesenchymal stem cells are capable of homing to the ... Distribution of MSCs in vivo Gross presentations of femoral head Kodak DXS small animal imaging system is a real-time imaging system which can be used to observe the distribution of cells in living...

Ngày tải lên: 25/10/2012, 11:18

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... on the reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, ... manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby in 1978 (Steptoe and Edwards...

Ngày tải lên: 22/03/2014, 09:20

... retardation due to dysregulation of gene expression [29] ESC, embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent stem cell; miRNA, microRNA complex to control ... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells ... programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human -induced pluripotent...

Ngày tải lên: 11/08/2014, 12:21

... use C46-transfected autologous stem cells in up to 10 patients with an independent indication for autoHSCT, i.e., high-risk AIDS-related lymphoma Use of autologous stem cells for gene transfer ... in order to avoid detrimental effects to volunteer-unrelated donor recruitment Using HIV resistance transgenes in stem cells Another approach to the provision of HIV-resistant blood cells is ... considerations apply to the described gene therapeutic approach, which may be adopted more widely due to the opportunity to use autologous stem cells Further experiences with CCR5-targeted stem cell therapies...

Ngày tải lên: 20/06/2014, 08:20

... circumstance, the treatment of MI by using MSCs showed poor survival of transplanted cells In addition to the quick loss of cells within 24 h of transplantation caused by cell leakage into the extra myocardial ... Preparation of MSCs MSCs were isolated from bone marrow of adult SpragueDawley male rats and expanded according to reported protocols [2,4] Whole marrow cells were cultured at a density of × 10 cells/ cm ... inflammatory response mediators and proapoptotic factors in the ischemic myocardium It has been showed that inflammatory process after MI peaks at week[12], and apoptosis is a major factor causing...

Ngày tải lên: 10/08/2014, 05:21

... Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from whole human ... differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation of HUMSCs ... differentiated human umbilical cord mesenchymal stem cells We assessed viability of the cells of sheets at room temperature No indication of cell apoptosis was noticed from the PNIPAAm coated surfaces...

Ngày tải lên: 10/08/2014, 05:21

... Project of the Japanese Ministry of Public Welfare and Labor Author details Dept of Surgery I, Tokyo Medical University, Tokyo, Japan 2Diagnostic Pathology, Division, Tokyo Medical University, Tokyo, ... time window in the profile mode at the resolution of 30 000 in the m/z range of 400 to 1600 The sets of acquired high-resolution MS and MS/MS spectra for peptides were converted to single data files ... immunohistochemically verified using a total of 10 cases for each group We assessed immunoreactivity with the percentage of immunopositive area and staining intensity compared to those of positive-control...

Ngày tải lên: 10/08/2014, 09:22

... potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma Curr Top ... (Professor, Department of Pathology, University of Manitoba, Canada) for facilitating preparation of this manuscript 10 11 12 13 14 15 Author details Key Laboratory of Pathobiology, Ministry of ... Liu CW: Induced endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV...

Ngày tải lên: 10/08/2014, 10:20

... immunoregulatory properties encouraged a possible use of these cells to modulate autoimmune responses and in the treatment of autoimmue diseases [12,13] To date, the experience of MSCs in the treatment of ... China 5Department of Rheumatology, The Affiliated Hospital of Nantong University, 20 Xi Si Road, Nantong, 226001, PR China 6Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical ... inflammatory responses in CIA There were reduced levels of proinflammatory cytokines and chemokines (TNF-a, IL-6 and MCP-1) and increased levels of the anti-inflammatory/regulatory cytokine (IL-10)...

Ngày tải lên: 12/08/2014, 15:22

... generation of large quantities of stem cells difficult One of the most-well studied is the adult hematopoietic stem cells, which give rise to all the blood cells Hematopoietic stem cells are in ... donor ES cells in vitro reprogramming of mature mouse fibroblasts into pluripotent stem cells (induced pluripotent stem cells, iPS cells) was first achieved using retroviral 26 transduction of four ... to cancer gene therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells...

Ngày tải lên: 09/09/2015, 18:56

... mesenchymal and inflammatory cells, osteoblasts Differentiation of undifferentiated mesenchymal cells into chondrocytes and osteoblasts and osteoprogenitor cells into osteoblasts Targeted cells: mesenchymal ... work at the damaged site Stem cells stored in the niche are exposed to an array of soluble chemokines, cytokines, growth factors, as well as insoluble transmembrane receptor ligands and ECM proteins ... osteoblasts, Targeted cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal...

Ngày tải lên: 11/09/2015, 09:57

... the stem cells to undergo apoptosis in an altruistic manner to prevent passing the DNA damage to their daughter cells Loss of stem cells will be compensated by expansion of the remaining stem cells ... identified to mark intestinal stem cells in a more specific manner [36] Transcriptome of intestinal stem cells have been probed by microarray [36] and culture of the stem cells has been reported to be ... 1.5.1 Location of intestinal stem cells Historically, there have been two schools of thought regarding the location of intestinal stem cells One school believes that the stem cells are located...

Ngày tải lên: 11/09/2015, 10:04

... A total of 33 primers were selected to determine the pluripotent and differentiated states of the cells (refer to Table 2) 21 primers were chosen that define the pluripotent states of the cells ... a mixture of cells It is also important to note that CDy9 does not only stain a colony of stem cells and allow easy identification and isolation but it also allows single stem cells to be detected ... were seeded onto mitomycin treated feeder cells for weeks before addition of 1uM of CDy9 and image acquisition Cells from dim population were seeded onto mitomycin treated feeder cells for weeks...

Ngày tải lên: 02/10/2015, 17:14

... staining and interaction with the stem cells and a better understanding of what is resulting in pluripotent stem cells to bind specifically to CDy9 as compared to the other cells This may eventually ... Disease-specific induced pluripotent stem (iPS) cells 2008 Cell 134(5): 877–886 9) N Maherali, K Hochedlinger Guidelines and Techniques for the Generation of Induced Pluripotent Stem Cells 2008 Cell Stem ... specificity of CDy9 to mESC cells compared to the other already found mESC probes Upon identifying the molecular binding partner of CDy9, the compound may also be translated to be a human pluripotent stem...

Ngày tải lên: 02/10/2015, 17:14