... lM) plus theophylline (0.5 mM), and H2O2 (2 mM) were included in the experimental medium from the time ofthe initiation ofthe influx experiment The influx was estimated from the slope ofthe uptake ... M) and used for estimation ofthe protein content using the Lowry procedure [19] and BSA as protein standard The residual 1000 lL ofthe suspension was centrifuged (20 000 g, 10 min) andthe ... within the initial 24 and from 25% to 50% within the subsequent five UV absorption (330 nm) ofthe samples and taurine standard (0.1 mM) were used for estimation ofthe taurine content in the samples...
... mechanisms for the determination ofthe position ofthe cleavage furrow How are microtubules associated with the equatorial region stabilized and how these microtubules determine the position ofthe cleavage ... released from the nucleus and accumulates at the cortex overlaying the nucleus, which recruits the other components to form the contractile structure In animal cells, the position ofthe cleavage ... She offered me the rigorous scientific training from the fundamental techniques to the creation ofthe great ideas Her encouragement and guidance helped me move through all the up and downs of...
... andthe use ofthe designation "European Venture Capital Fund" should be sanctioned by the prohibition ofthe use ofthe designation andthe removal ofthe venture capital fund manager ofthe ... 45/2001 ofthe European Parliament andofthe Council of 18 December 2000 on the protection of individuals with regard to the processing of personal data by the EU institutions and bodies and on the ... following the end ofthe financial year The report shall describe the composition ofthe portfolio ofthe qualifying venture capital fund andthe activities ofthe past year It shall contain the audited...
... human and rat CB1 receptor The CB2 antibody was raised against a sequence between the N-terminal andthe first transmembrane domain ofthe protein ofthe human and rat CB2 receptor The specificity of ... for AEA, PalEtn and 2AG, respectively Analysis of cannabinoid receptors and fatty acid amide hydrolase To determine the presence ofthe cannabinoid receptors (CB1 and CB2) andofthe fatty acid ... supported by the observation that preabsorbing ofthe CB1 antibody with its corresponding blocking peptide eliminated almost all ofthe staining of these bands (Fig 3A) The most abundant band in human...
... dysfunction SH3 and SH2 domain dysfunction Deletion of N-terminal, SH3 and SH2 domain of active n-Src Deletion of N-terminal, SH3 and SH2 domain of kinase-dead n-Src of PP3, the inactive form of PP2, ... upstream ofthe conserved PXXP motif ofthe SH3 ligand The D99N mutation prevents formation of this salt bridge and disrupts the SH3 binding specificity R175 (corresponding to R183 in n-Src) in the ... R175K mutations therefore inhibit interactions with ligands ofthe SH3 and SH2 domains, respectively, both intra- and intermolecularly, and thereby disrupt the overall functions of Src kinase [3,18–21]...
... Additionally, because the demand ofthe chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin ofthe signal, and thus regulation at the Tic complex could be one ofthe fastest ... which anchor the protein in the membrane The position and function ofthe long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic ... description of recent advances in the understanding ofthe channel-, motor- and regulatory components ofthe Tic complex For reference, some ofthe available knowledge, including the proposed function of...
... PARLIAMENT ANDTHE COUNCIL OFTHE EUROPEAN UNION, Having regard to the Treaty on the Functioning ofthe European Union, and in particular the second paragraph of Article 177 thereof, Having regard to the ... respect the principle of subsidiarity as the tasks ofthe ERDF are set out in the Treaty andthe policy is implemented in accordance with the principle of shared management and respecting the institutional ... increasing the use of conditionalities and simplifying the delivery are among the major hallmarks ofthe proposal This Regulation sets out the provisions governing the Cohesion Fund, and repealing Regulation...
... establish the importance of GSK3 in the acute regulationof glucose transport in terms of its regulatory control of GS in muscle and fat cells we have investigated the effects of Li andthe anilinomaleimide, ... elements implicated in theregulationof GSK3 and GS To further understand the effects of Li and SB-415286 on cell signalling events we assessed their effects and that of insulin on p70S6K, PKB, ... and myotubes with antibodies against GSK3a and b Fig 1B shows that whilst preadipocytes express both GSK3 isoforms, the abundance ofthe b isoform declines by 85%, whereas that ofthe a isoform...
... authority ofthe Member State in whose territory the investigation is to be carried out ofthe investigation andofthe identity ofthe authorised persons Officials ofthe competent authority ofthe ... proportionate to the nature and seriousness ofthe breach, the duration ofthe breach andthe economic capacity ofthe credit rating agency concerned Detailed criteria for establishing the amount ofthe fines ... the adoption of a regulation on fees andthe amendment ofthe annexes (19) Directive 95/46/EC ofthe European Parliament andofthe Council of 24 October 1995 on the protection of individuals...
... corporation Office ofthe Comptroller ofthe Currency Office of Thrift Supervision savings and loans Securities and Exchange Commission Summary of Deposits Department ofthe Treasury This is a work ofthe ... FDIC, the Federal Reserve, the Office ofthe Comptroller ofthe Currency (OCC), the Office of Thrift Supervision (OTS) and SNL Financial relating to the number of exempt institutions, their geographic ... relating to the types of activities in which such institutions may engage, the availability and allocation of credit, the stability ofthe financial system andthe economy, andthe safe and sound...
... effects of ligand-activated RARa and RXRa on the promoter activity ofthe hGnRH II gene in TE671 and JEG-3 cells In vivo effect of RARa and RXRa with their ligands, ATRA and 9-cisRA, on the promoter ... Fig therefore imply that ligand-bound RAR and RXR are responsible for the repression of GnRH II gene in TE671 cells On the other hand, over-expression of RARs together with the application of ... to the results observed in placental cells andthe current paradigm ofthe role of RA-induced activation, the application of RAs and introduction of ligand-activated RAR ⁄ RXR heterodimer further...
... known that the length ofthe CAG repeat affects the onset of HD in humans and in mice, we first confirmed that the rate of decrease in steady-state CB1 mRNA was dependent on the length ofthe CAG ... repeat and relative expression ofthe HD gene We then determined the structure ofthe mouse CB1 gene and determined that transcription ofthe CB1 gene was affected in striatal neurons of transgenic ... levels in the medial striatum of any ofthe wild-type and HD mice examined The optical density ofthe hybridization signal in the lateral striatum, corrected by subtracting the signal in the medial...
... investors andthe use ofthe designation "European Social Entrepreneurship Fund" should be sanctioned by the prohibition ofthe use ofthe designation andthe removal ofthe EuSEF manager ofthe register ... end ofthe financial year The report shall describe the composition ofthe portfolio ofthe EuSEF andthe activities ofthe past year It shall contain the audited financial accounts for the EuSEF ... relevance) THE EUROPEAN PARLIAMENT ANDTHE COUNCIL OFTHE EUROPEAN UNION, Having regard to the Treaty on the Functioning ofthe European Union, and in particular Article 114 thereof, Having regard to the...
... [86] Nevertheless, the observed homodimerization ofthe GCD andthe metal ion dependence ofthe catalytic activity support the integrity ofthe expressed proteins As expected from the high sequence ... NPRA andthe ligand ANP must be localized on the luminal side ofthe renal tubule (but not on the basolateral side, where the chloride concentration is stable at 90–110 mm) There is a plethora of ... view ofthe chloridebinding site in apo-ECD [18,34] Chloride is hydrogen bonded to the hydroxyl group of Ser53, andthe backbone NH moieties of Gly85 and Cys86 The binding site also contains the...
... and 71 Considering the location ofthe N-terminal extremity of DNter2 on the structure of CARP andthe presence ofthe potential cleavage sites, we identified the localization ofthe cleavage site ... a deregulation ofthe NF-jB pathway and an increase in muscle fiber apoptosis [8] The participation of NF-jB signaling in the pathogenesis of LGMD2A is therefore an interesting possibility The ... activates the interaction of a protein complex with the kinase domain of titin, reducing the nuclear translocation ofthe transcription factor SRF and impeding gene transcription [16] In the elastic...
... neurons project from the ARC to the PVN, andthe other in which they project to the MPA, bed nucleus ofthe stria terminalis and lateral septal nucleus Central administration of GALP activates neurons ... regions ofthe rat brain Injection of GALP into the third ventricle induces c-Fos expression, a marker of cell activation, in the horizontal limb ofthe diagonal band of Broca, POA, ARC and median ... and stimulates thermogenesis in rodents The short-term orexigenic actions of GALP are mediated via NPY andthe orexinergic pathway in the rat The long-term anorectic and thermogenic actions of...
... equivalent to the R302Q substitution in c2, the dominant isoform ofthe c-subunit in the heart The R302Q mutation, together with several other substitutions identified in CBS domains ofthe c2 gene, ... to study the effect ofthe mutations within AMPK c3 on the activity ofthe enzyme, we introduced several missense mutations into the c3 gene (Fig 2), and investigated the effect of these substitutions ... heterotrimers with the a2 and b2 isoforms [23] The present study describes bacterial expression of AMPK a2b2c3 trimers, and provides the first characterization ofthe enzyme kinetic parameters and regulation...
... modulation of CTGF and Cyr61 gene expression in vitro and in vivo Mechanical regulation in bone and cartilage Cartilage and bone provide ideal tissues for the study ofthe mechanical regulationand ... represent another example of situation in which the mechanical regulationof Cyr61 and CTGF is of relevance TGF-b which is a major profibrotic and fibrogenic molecule, is one ofthe potent inducers of CTGF ... complexity and variability oftheregulationof cellular communication Collectively, both the expression and function ofthe immediate early genes Cyr61 and CTGF cannot be separated from the mechanically...
... terms ofthe presentation ofthe problems and in relation to the options andi the policy choice The resubmitted version of 14 November contains the following improvements: In line with the IAB’s ... state aid rules andthe EU Treaty) While the effectiveness of these other measures will have a strong impact on the take-up ofthe measures identified here, the justification for these latter measures ... certain risks of early intervention; • Clarifying further the phasing of options, their interactions, the costs and benefits ofthe approach to phasing of options, e.g under sections 6, and 8; • Outlining...
... cleavage ofthe p220 component ofthe cap-binding protein complex [32], resulting in the shut off of host protein synthesis On the other hand, the function of L ofthe cardioviruses is not well ... persistence L and picornaviruses The aphthoviruses and caridioviruses are the only members ofthe Picornaviridae family that contains an L coding region at the most 5' terminus ofthe ORF [13] In the case ... inhibit the iron/ferritinmediated activation of NFκB [34] The functions of L of Page of (page number not for citation purposes) Journal of Neuroinflammation 2006, 3:19 TMEV andthe other cardioviruses...