pp2a regulatory subunit transgenic mice

... N-terminal regions of the a-subunits serves as catalytic domains, and the C-terminal regions of the a-subunits and the b-subunits act as regulatory domains [10,11] The regulatory domains of AK contain ... composed of a catalytic domain from BsAKII and regulatory domains (a regulatory domain in the a -subunit, and a b -subunit with the same sequence as the regulatory domain) from TtAK, improved thermostability ... TtAK subunits of SDS ⁄ PAGE in (B) The a-subunits and b-subunits are indicated by a solid line with circles and a dotted line with squares, respectively (E) Densitometric calibration of TtAK subunits...

Ngày tải lên: 18/02/2014, 08:20

... b subunits to form dimers composed of two b subunits, of two btes subunits and, of the mixture of b/btes subunits These interactions are weaker than interaction of a subunit with btes or b subunit, ... with the a subunit are the canonical features of the regulatory subunit of CK2 CK2btes protein interacts with CK2 a subunit in yeast two-hybrid system To examine whether the CK2 a subunit is ... testis-specific isoform of the CK2 regulatory subunit in normal males The CK2 regulatory subunit is ubiquitous among eukaryotes, but an amino-acid sequence of different b subunits is far less conservative...

Ngày tải lên: 21/02/2014, 15:20

... that PP2A may play important roles in developing eyes, and the functions of PP2A appear to be highly regulated by various regulatory subunits [17] The mRNA isoforms of the PP2A Asubunit and B -subunit ... gene promoters Among the various PP2A genes, we found that the Ppp2r5c (PP2A- B56c), Ppp2r2b (PP2A- B55b; data not shown) and Ppp3cc (protein phosphatase 3, catalytic subunit c; data not shown) genes ... novel regulatory subunit of protein phosphatase 2A FEBS Lett 460, 462–466 Zolnierowicz S, Csortos C, Bondor J, Verin A, Mumby MC & DePaoli-Roach AA (1994) Diversity in the regulatory B-subunits...

Ngày tải lên: 06/03/2014, 02:20

... Catalytic subunit Core regulatory subunit of Pph3 ⁄ Ppp4c Regulatory subunit of Pph3 ⁄ Ppp4c Core regulatory subunit of Ppp4c Catalytic subunit Catalytic subunit Catalytic subunit Common regulatory subunit ... Ppp4 and Pph3 subunit structures may thus resemble those of PP2A and Pph21 ⁄ Pph22, where a core regulatory subunit forms a complex with the catalytic subunit to which a third variable subunit may ... the identification of two regulatory subunits of Ppp4c that not interact with the closely related PP2Ac, namely R1 (105 kDa) [15] and R2 (55 kDa) [16] These regulatory subunits form independent...

Ngày tải lên: 07/03/2014, 12:20

... T cells from 21SHL transgenic mice and 21SHL-CL transgenic mice were impaired in their proliferative response to CD3e antibody stimulation to the same extent as in WASP15 transgenic T cells [24] ... anti-WASP scFv transgenic T and B cells, and lymphoid development in anti-WASP scFv transgenic mice (A) T-cell proliferation Splenic T cells from anti-WASP scFv 21SHL transgenic, 21SHL-CL transgenic, ... of at least three male mice for each analysis at the age of weeks purified T cells from spleens of wild-type, WASP15 transgenic, 21SHL transgenic and 21SHL-CL transgenic mice were stimulated with...

Ngày tải lên: 16/03/2014, 14:20

... neurons of transgenic HD mice Experimental procedures Animals Two transgenic HD mice colonies were established and maintained by crossing hemizygous R6/2 or R6/1 males with CBAxC57BL/6 females Mice ... striatum of wild-type mice and 3-week-old R6/1 and R6/2 transgenic HD mice (Fig 1A) The CB1-specific hybridization signal was decreased in the lateral striatum of older transgenic HD mice There was no ... derived from the striatum of wild-type and HD transgenic mice Striatal RNA was extracted from 3-, 5-, 6-, and 12 week-old wildtype and R6/1 transgenic HD mice (n ¼ per age and genotype) Three gene-specific...

Ngày tải lên: 16/03/2014, 18:20

... the cTnTR141W transgenic mice and CYP2E1 transgenic mice beginning at months of age, with respective death rates of 17.5% in cTnTR141W transgenic mice and 13.75% in CYP2E1 transgenic mice being ... also triggered in CYP2E1 transgenic mice and cTnTR141W transgenic mice, and the apoptosis of myocytes was increased in CYP2E1 transgenic mice and cTnTR141W transgenic mice (Fig 6), suggesting ... WT, cTnTR141W, CYP2E1(#26) and CYP2E1(#36) transgenic mice (Fig 3A and Table 1) The cTnTR141W transgenic mice and the two lines of CYP2E1 transgenic mice exhibited the DCM phenotype (i.e chamber...

Ngày tải lên: 22/03/2014, 16:20

... tg-ArcSwe mice (data not shown), consistent with the observed difference in escape latency Representative swim paths of nontransgenic and transgenic mice are shown in Fig 4B Nontransgenic mice clearly ... FEBS 999 Amyloid-b protofibrils in transgenic mice A A Lord et al Fig Spatial learning and memory in tg-ArcSwe mice Transgenic (tg-ArcSwe) and nontransgenic (non-tg) mice were tested in the Morris ... procedures Histology and image analysis Transgenic mice APP transgenic mice with the Swedish (K670N, M671L) and Arctic (E693G) mutations (tg-ArcSwe mice) [20], non- 1002 Mice were anesthetized with 0.4...

Ngày tải lên: 23/03/2014, 06:20

... and the transgenic N171-82Q mice (B–D) Measured (circle for control, triangle for transgenic N171-82Q mice) and simulated (solid line for control, dashed line for transgenic N171-82Q mice) curves ... the transgenic HD mice (Table 2), whereas no change was detected in the unaffected regions (data not shown) In the 3-NP mice, the activity of HK had increased more than in the transgenic mice ... mgÆkg)1 into CFLP mice (twice a day) Mice from the same strain received NaCl ⁄ Pi vehicle and were used as a control Mice were sacrificed after the seventh 3-NP injection Transgenic mice expressing...

Ngày tải lên: 23/03/2014, 10:20

... its biological properties PKA regulatory subunits control the release of catalytic subunits from the inactive tetramer complex upon binding of cAMP to the regulatory subunit- dimer Thus, we examined ... they contained 1500 bp DNA, 1120 bp of which encoded for the C-terminal portion of the RIIa regulatory subunit of PKA No hepatic cell line expressing HIP/PAP was available Thus we have established ... HIP/PAP and RIIa were further analyzed using a fractionation method (Fig 1B) The regulatory RIIa and the catalytic Ca subunits of PKA were detected in the 100 000 g ultracentrifugation pellet and...

Ngày tải lên: 23/03/2014, 13:20

... days Results obtained with transgenic (n ¼ 11) and control (n ¼ 11) mice are shown in black and gray, respectively 150 s ± 16 versus 146 s ± 18 for transgenic and control mice, respectively) Similarly, ... recombination pattern in the transgenic mouse line, by analyzing one transgenic line in detail (BAC-DATiCre line), and crossing this line with the R26R reporter line [9] In transgenic animals carrying ... populations Efforts have recently been made to generate transgenic mice expressing the recombinase in restricted neuronal populations [8] We generated a transgenic mouse line [bacterial artificial chromosome...

Ngày tải lên: 30/03/2014, 08:20

... in all homologs) q FEBS 2001 PKA regulatory subunit from T brucei (Eur J Biochem 268) 6201 Fig Sequence comparison of cNMP-binding domains of PKA regulatory subunits and of protein kinase G cNMP-binding ... further difference between TbRSU and the PKA regulatory subunits from other eukaryotes is seen in Val319 All cAMP-binding domains of the regulatory subunits carry an alanine residue at this position, ... family of regulatory enzymes Annu Rev Biochem 59, 971 –1005 19 Su, Y., Dostmann, W.R.G., Herberg, F.W., Durick, K., Xuong, N.H., Ten Eyck, L., Taylor, S.S & Varughese, K.I (1995) Regulatory subunit...

Ngày tải lên: 31/03/2014, 23:20

... of month old APP [V717I] transgenic mice, while absent in non-transgenics (data not shown) and most significantly increased in the brain of old APP [V717I] transgenic mice at 16 months (Figure ... cortical GFAP-immunostaining was practically absent in non -transgenic control mice at months (data not shown), whereas APP [V717I] transgenic mice had randomly distributed foci of astrocytes strongly ... Methods Animals Transgenic mice expressing APP [V717I] under the mouse thy1 gene promoter in the FVB/N genetic background [6] aged and 16 months were used in this study with nontransgenic mice of the...

Ngày tải lên: 19/06/2014, 22:20

... affinity interactions Experimental design Transgenic mice Tg2576 APP mice [2]) were acquired from the breeding colonies at the University of South Florida Multiple mice were housed together whenever ... (untreated) hippocampal regions of 20 mo old APP transgenic mice Panels A, C, and E show total Aβ staining in right hippocampal regions of 20 mo old APP transgenic mice receiving intracranial injection ... hippocampal regions of 20 mo old APP transgenic mice Panels A, C, and E show total thioflavine S staining in right hippocampal regions of 20 mo old APP transgenic mice receiving intracranial injection...

Ngày tải lên: 19/06/2014, 22:20

... parallel Materials and methods Transgenic animals The heterozygous Tg197 transgenic mouse was generated and described previously by our research group [3] Briefly, Tg197 mice carry a human TNF transgene ... weeks of age all mice were killed and the hind ankle joints were removed for histology Histologic processing and scoring of joints Ankle joints were removed from the transgenic mice and were fixed ... only 11% of the joints of the mice treated with low dose diacerein and none of the joints of mice treated with dexamethasone or anti-TNF had a similar score, whereas in mice treated with methotrexate...

Ngày tải lên: 09/08/2014, 01:23

... between young and aged mice Cartilage in young mice treated with anti-TNF-α was significantly improved relative to saline-treated and baseline mice By comparison, treatment of aged mice with anti-TNF-α ... in both young and aged mice, and a significant reduction in cartilage degradation in comparison with baseline was maintained in young mice Cartilage degradation in aged mice treated with antiTNF-α ... anti-TNF-α monoclonal antibodies reverses established arthritis in Tg197 mice (a) Young mice (at to weeks of age) or (b) aged mice (at 27 to 28 weeks of age) with established arthritis were randomized...

Ngày tải lên: 09/08/2014, 06:22

... macrophages from HLA-DR1 transgenic mice for antigen presentation of both the glycosylated and non-glycosylated forms of the arthritogenic CII259– 273 epitope to CD4 T cells Treatment of mice with an inhibitor ... chromatography Animals In all experiments described in this study we used previously reported mice transgenic for HLA-DR1 on a major histocompatibility complex (MHC) class II-deficient background ... glycosylated and non-glycosylated arthritogenic epitope CII259–273 generated from HLA-DR1 -transgenic mice [27], which allowed us to test the effect of post-translational modification on uptake...

Ngày tải lên: 09/08/2014, 08:22

... mice were to weeks old; wild type mice and the recipient mice, both HCV transgenic and non -transgenic mice, were to months old The establishment and characterization of these HCV transgenic mice ... immunized mice into HCV transgenic mice Our results showed that the carboxyfluorescein succinimidyl ester (CFSE)-labeled T cells from HCV immunized mice homed to the liver of HCV transgenic mice, ... non -transgenic recipient mouse groups when they received transfers from nonimmunized donors In contrast to wild-type mice, donor cells from immunized mice homed to the liver of transgenic mice...

Ngày tải lên: 13/08/2014, 13:20

... shuttle vector [18] 2.2 Generation of transgenic mice Isolation and micro-injection of the modified BAC insert was performed as previously described [15] Transgenic mice were identified both by Southern ... in transgenic mice [9] Expression of this gene was assessed by RT-PCR in the three transgenic lines obtained with the modified BAC It revealed that the 244 S Soulier et al Figure Detection of transgenic ... transgenic mice and CAT expression analysis A: Detection of transgenic mice by Southern analysis of PstI-digested genomic DNA The origin of the genomic DNAs are indicated on the top lane N.Tg.: non-transgenic...

Ngày tải lên: 14/08/2014, 13:22

... Additionaltumors in differentiallymice micehyperplastic mammary at thehere forMMTV-Wnt- 1transgenic in MMTV-Wnt-1 mice mice transgenic Filetransgenic/Pten three-fold transgenic/ Ptentissues than fromMMTV-Wnt-1 ... MMTV-Wnt-1 and MMTV-Neu transgenic mice MMTV, mouse mammary tumor virus; NeuT, mammary tumors from MMTV-Neu transgenic mice; WntT, mammary tumors from MMTV-Wnt-1 transgenic mice reference RNAs, ... glands from nontransgenic mice and in hyperplastic mammary glands from MMTV-Wnt-1 transgenic mice, but was readily and widely detected in the tumor samples from MMTV-Wnt-1 transgenic mice (Figure...

Ngày tải lên: 14/08/2014, 14:22