phần ii kỹ thuật nuôi trồng hải sản

... which activates NF-B via TRAF-2 [35], (ii) TRAF-6; an NFB regulator that plays a critical role in human autoimmune diseases including arthritis [36], (iii) TNF-receptor superfamily member TNFRSF21; ... manufacturer’s instructions Gene expression was subsequently analyzed with qRTPCR by using SuperScript III Platinum Two-Step qRTPCR Kit with SYBR Green (Invitrogen), according to the manufacturer’s instructions, ... http://arthritis-research.com/content/13/4/R129 IP (i) (ii) IL ID -1 R- + IL ID -1 R- + 10 02 -1 IL B Ct rl -phospho-c-Jun (Ser73) (i) (ii) IP - phospho-ATF-2 (Thr76) Figure Evaluation of JNK...

Ngày tải lên: 12/08/2014, 17:22

... activation and IjBa degradation [48] Phosphorylation of serine 529 of p65 is mediated by casein kinase II, but is prevented in nonstimulated cells by the interaction with IjBa [49] Thus, similar to PKAc-mediated ... PKAc-mediated phosphorylation of serine 276 of p65, phosphorylation on serine 529 by casein kinase II occurs in the cytoplasm after TNF-induced degradation of IjBa Again, p65 phosphorylation on serine ... structural and functional analyses of the TRADD–TRAF2 interaction Cell 101, 777–787 10 Chen NJ, Chio II, Lin WJ, Duncan G, Chau H, Katz D, Huang HL, Pike KA, Hao Z, Su YW et al (2008) Beyond tumor...

Ngày tải lên: 28/03/2014, 23:20

... C, Parmentier M, Serhan CN, Murphy PM: International Union of Basic and Clinical Pharmacology LXXIII Nomenclature for the formyl peptide receptor (FPR) family Pharmacol Rev 2009, 61:119-161 John ... Neurotherapeutics 2010, 7:354-365 11 Serhan CN, Chiang N, Van Dyke TE: Resolving inflammation: dual antiinflammatory and pro-resolution lipid mediators Nat Rev Immunol 2008, 8:349-361 12 Claria J, Serhan...

Ngày tải lên: 19/06/2014, 22:20

... Comparison of the degradation of type II collagen and proteoglycan in nasal and articular cartilages induced by interleukin-1 and the selective inhibition of type II collagen cleavage by collagenase ... Cook M, Chin VI, Gowen M, Grodzinsky AJ, Lark MW: Down-regulation of chondrocyte aggrecan and type -II collagen gene expression correlates with increases in static compression magnitude and duration ... 2001, 5:329-343 Hollander AP, Pidoux I, Reiner A, Rorabeck C, Bourne R, Poole AR: Damage to type II collagen in aging and osteoarthritis starts at the articular surface, originates around chondrocytes,...

Ngày tải lên: 09/08/2014, 01:21

... Rheum Dis 2007, 66(Suppl 3):iii52-5 Brzoska T, Luger TA, Maaser C, Abels C, Böhm M: Alphamelanocyte stimulating hormone and related tripeptides: biochemistry, antiinflammatory and protective ... nonspecific binding were from Becton, Dickinson and Company (Franklin Lakes, NJ, USA) The mAbs antiIL-1β and anti-IL-8 and the specific enzyme-linked immunosorbent assay (ELISA) for IL-1β, IL-8, ... for 30 minutes After washing, the cells were analyzed by flow cytometry (Becton Dickinson FACS II; Becton, Dickinson and Company) A relative measure of antigen expression was obtained using the...

Ngày tải lên: 09/08/2014, 14:22

Ngày tải lên: 10/08/2014, 05:21

... were allowed to be polymerized by addition of TEMED and 10% ammonium persulfate The Mini-PROTEAN III (Bio-Rad) was used to cast the polyacrylamide gel When the polymerization is completed, the ... coverslips were viewed using an inverted Zeiss microscope (Axio Observer D1) with α Plan-Fluar 100X/1.45 III objective Statistics Statistical analyses were performed with Student’s t test Differences were ... Gardel ML: Transient frictional slip between integrin and the ECM in focal adhesions under myosin II tension Curr Biol 2010, 20:1145-1153 15 Yeung T, Georges PC, Flanagan LA, Marg B, Ortiz M, Funaki...

Ngày tải lên: 10/08/2014, 10:20

... T-cell lines MT4-IIIB and MT4-LTR-EGFP-IIIB, where the expression of HIV encoded proteins in >99% of cells could be detected by intracellular p24 staining (not shown) In MT4-LTR-EGFP-IIIB cells, HIV ... cells Persistently infected MT4-IIIB and MT4-LTR-EGFP-IIIB cells were generated by infection of parental MT-4 or MT4-LTR-EGFP cells, respectively, with HIV-1IIIB at an MOI of 0.1 The cytopathic ... MT4-CMV-EGFP control cells CC50 [μM] Cytotoxicity on MT4LTR-EGFP-IIIB HIV-1 producing cells CC50 [μM] Ctotoxicity on MT4-LTREGFP-IIIB cells in presence of 0.1 μM DRV CC50 [μM] Enhancement of RTDimerization...

Ngày tải lên: 13/08/2014, 01:20

... 1540–1546 Ye, X., Ishii, I., Kingsbury, M.A & Chun, J (2002) Lysophosphatidic acid as a novel cell survival/apoptotic factor Biochim Biophys Acta 30, 108–113 Noguchi, K., Ishii, S & Shimizu, T ... lysophospholipids and their G protein-coupled receptors Exp Cell Res 253, 230–238 Contos, J.J., Ishii, I & Chun, J (2000) Lysophosphatidic acid receptors Mol Pharmacol 58, 1188–1196 Kranenburg, O ... Wysolmerski, R (2000) Endothelial cell retraction is induced by PAK2 monophosphorylation of myosin II J Cell Sci 113, 471–482 13 Parsons, J.T., Martin, K.H., Slack, J.K., Taylor, J.M & Weed, S.A...

Ngày tải lên: 30/03/2014, 13:20

... from the Comunidad Autónoma de Madrid (S-SAL/0261/2006) and by RETICS, Instituto de Salud Carlos III (Red Española de Esclerosis Múltiple REEM; RD 07/0060/ 0010) Author details Neuroimmunology ... associated to MS and other neurodegenerative diseases [rev [10,11]] Both AEA and 2-AG possess antiinflammatory and neuroprotective properties against harmful insults [12-16] Controversial changes ... Characteristics of cloned cerebrovascular endothelial cells following infection with Theiler’s virus II Persistent infection J Neuroimmunol 1995, 62:127-135 39 Inoue A, Koh CS, Yamazaki M, Ichikawa...

Ngày tải lên: 19/06/2014, 22:20

... panel II and 7b, panel II) of the western blot results confirmed that resveratrol and/or curcumin increase the expression of collagen type II (Figure 7a, panel II) and Sox9 (Figure 7b, panel II) ... effect on the expression of IKK protein (Figure 11b, panels II and III) Discussion The aim of the present study was to determine if the antiinflammatory and anti-apoptotic effects of resveratrol ... IL-1β or resveratrol had no direct effect on the expression of IKK protein (Figure 11a, panels II and III) Curcumin but not resveratrol inhibits IL-1β-induced IκBα kinase activation Since we could...

Ngày tải lên: 09/08/2014, 14:22

... Cancer 9, 537–549, doi:nrc2694 [pii] 10.1038/ nrc2694 Davis RJ (2000) Signal transduction by the JNK group of MAP kinases Cell 103, 239–252, doi:S00928674(00)00116-1 [pii] Yan M, Dai T, Deak JC, Kyriakis ... [pii] 10.1038/ng1963 13 Bentires-Alj M & Neel BG (2007) Protein-tyrosine phosphatase 1B is required for HER2 ⁄ Neu-induced breast cancer Cancer Res 67, 2420–2424, doi: 0008-5472.CAN-06-4610 [pii] ... M107653200 [pii] 24 Sekine Y, Tsuji S, Ikeda O, Sato N, Aoki N, Aoyama K, Sugiyama K & Matsuda T (2006) Regulation of STAT3-mediated signaling by LMW-DSP2 Oncogene 25, 5801–5806, doi:1209578 [pii] 10.1038/sj.onc.1209578...

Ngày tải lên: 16/02/2014, 14:20

... domain of MT1-MMP LLY570-573AAA MT1/MYC and G2A GRASP55F mutants were generated using the QuikChange II site-directed mutagenesis kit (Stratagene, Amsterdam, the Netherlands) Full-length rat GRASP55 ... imposed by the three-dimensional extracellular matrix Cell 114, 33–45 Ueda J, Kajita M, Suenaga N, Fujii K & Seiki M (2003) Sequence-specific silencing of MT1-MMP expression suppresses tumor cell migration...

Ngày tải lên: 18/02/2014, 04:20

... histocompatibility complex (MHC) class II determinants without interferon-gamma pretreatment: synergistic effects of interferon-gamma and tumor necrosis factor on MHC class II induction Eur J Immunol 19, ... release and leukocyte migration by lipopolysaccharide-stimulated primary human lung alveolar type II epithelial cells and macrophages J Immunol 178, 463–473 Gold R, Toyka KV & Hartung HP (1995) ... induced to produce cytokines and chemokines, to express major histocompatibility complex (MHC) class II molecules and adhesion molecules, and to serve as antigen-presenting cells [18–20] These chemokines...

Ngày tải lên: 18/02/2014, 18:20

... complex(es) in prepubertal rat Sertoli cells Endocrinology 135, 2479– 2487 Uchijima M, Sato H, Fujii M & Seiki M (1994) Tax proteins of human T-cell leukemia virus type and induce expression of ... Chem 278, 39413–39421 Reid T, Furuyashiki T, Ishizaki T, Watanabe G, Watanabe N, Fujisawa K, Morii N, Madaule P & Narumiya S (1996) Rhotekin, a new putative target for Rho bearing homology to...

Ngày tải lên: 19/02/2014, 05:20

... constitute a very complex signalling network (Fig 1) We hypothesized that this network is capable of quasiintelligent behaviour, for instance by making the output (ERK phosphorylation) dependent on the ... with Lumi-LightPLUS Western Blotting Substrate (Roche) Signals were detected with a FluorSTM MultiImager (Bio-Rad) and quantified using the MULTI-ANALIST software (Bio-Rad) All measurements were...

Ngày tải lên: 19/02/2014, 16:20

... FOXO transcription factors in pancreatic b cells J Endocrinol 193, 195–207 17 Nakae J, Biggs WH III, Kitamura T, Cavenee WK, Wright CV, Arden KC & Accili D (2002) Regulation of insulin action...

Ngày tải lên: 06/03/2014, 02:20

... ZNHIT-1 by Rev-erbb to the apoCIII promoter and its effect on the apoCIII promoter (A) Recruitment of ZNHIT-1 by Rev-erbb to the apoCIII promoter A ChIP assay of the apoCIII promoter was performed ... endogenous ApoCIII were transiently transfected with HA-Rev-erbb and either Myc-ZNHIT-1 or empty vector, together with the pGL3-apoCIII-pro reporter, which contains the promoter of the human apoCIII gene ... Recruitment of ZNHIT-1 by Rev-erbb to the apoCIII promoter and its effect on the apoCIII promoter Rev-erbb has been reported to repress the expression of apoCIII [17] To extend this observation, Rev-erbb...

Ngày tải lên: 07/03/2014, 05:20

... phase II clinical trials for osteoarthritis However, it was recently withdrawn for treatment of rheumatoid arthritis because of evidence of abnormal liver toxicity in a long term phase II animal ... was monitored continuously for 10 by measuring fluorescence (kex355, kem460) using a FlexStationII384 All the inhibitors were dissolved and diluted in Me2SO before addition to the assay mixture; ... Alzheimerassociated presenilins during apoptosis by a Caspase-3 family protease Science 277, 373–376 11 Niikura T, Hashimoto Y, Tajima H & Nishimoto I (2002) Death and survival of neuronal cells exposed...

Ngày tải lên: 07/03/2014, 11:20

... transduction Science 252, 802–808 11 Riddle DL, Blumenthal T, Meyer BJ & Priess JR (1997) C elegans II Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 12 The C elegans Sequencing Consortium ... volatile anesthetic action in Caenorhabditis elegans Genetics 158, 643–655 19 Matsuki M, Kunitomo H & Iino Y (2006) Goalpha regulates olfactory adaptation by antagonizing GqalphaDAG signaling in Caenorhabditis...

Ngày tải lên: 07/03/2014, 11:20