non carbohydrate mediated interaction of lectins with plant proteins

... a profound effect on the strategies learners choose, with negative attitudes and beliefs often causing poor strategy use or lack of orchestration of strategies  Type of task: The nature of the ... new beginners to those with the highest levels of proficiency Such a study would help us to get a full idea of the impact of CS in learning vocabulary - 43 - The results of the questionnaire used ... the meanings of the parts to understand the meaning of the whole expression • Analyzing contrastively: Students compare elements (sounds, vocabulary, etc) of English words with those of Vietnamese...

Ngày tải lên: 07/11/2012, 14:54

... assigned with a relatively equal proportion of good, average and poor English proficiency students and these classes are at different levels of English From observation and interviews with teachers of ... student can be well catered for An area of research which has received a lot of attention in many areas of education is the issue of learning style The idea of learning style comes from general ... congress of the Communist Party of Viet Nam, the policy of DOI MOI (Renovation) was adopted New approaches to planning in all fields emerged, affecting all sectors of society Education is just one of...

Ngày tải lên: 07/09/2013, 13:48

... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin DISCUSSION The binding of decorin with fibrillar collagens has been extensively ... (1995) Modeling of the three-dimensional structure of proteins with the typical leucinerich repeats Structure 3, 867–877 Kajava, A.V (1998) Structural diversity of leucine-rich repeat proteins J Mol...

Ngày tải lên: 21/02/2014, 15:20

... (B), but with the use of Sepharose beads without dicalcin conjugated (C) Proteins were stained with silver Amino acid sequence analysis of 38 kDa and 35 kDa proteins During the course of this ... localizations and the effect of dicalcin on the activities of these annexins by measuring liposome aggregation Results Purification of binding proteins of dicalcin Binding proteins of dicalcin were searched ... that 35 kDa proteins contained proteolytic fragments of 38 kDa proteins: in the presence of protease inhibitors, the amount of 38 kDa proteins was larger than that in the absence of the inhibitors...

Ngày tải lên: 07/03/2014, 05:20

... property of DAPI could only be attributed to a direct interaction of the drug with the Klenow fragment A DNA -mediated step correlating with the different structural and binding characteristics of DAPI–template ... structure of DAPI-bound ATTtemplate with respect to its free form, which is consistent with the previously reported lower mobility of the free monomeric random coil structure of (ATT)6 in comparison with ... electrophoretic bands of (AAT)6, (ACT)6, (AGT)6, (ATG)6 Fig Effect of DAPI on the electrophoretic mobility of trinucleotide repeat DNA sequences (A) Nondenaturing 12% polyacrylamide gel migration of the 20...

Ngày tải lên: 16/03/2014, 23:20

... indicating that the interactions of these different proteins are likely to be indirect, depending on the presence of intact RNA as integral component of the RNP Association of 42Sp50 with mRNA has ... family of RNA binding proteins [31–33] Together with the findings obtained in the present study, interaction of VICKZ and poly(A) binding proteins thus appears to define a conserved feature of mRNPs ... variants of known localization proteins such as Vg1RBP expressed by means of oocyte microinjection Immunoprecipitation of such RNPs allowed for the identification of novel Vg1RBP-associated proteins...

Ngày tải lên: 23/03/2014, 03:20

... Journal compilation ª 2009 FEBS H Hemmi et al Interaction of EW29Ch with sugars Identification of sugar-binding sites The interaction of 15N-labeled EW29Ch with lactose, melibiose, galactose, methyl ... et al Interaction of EW29Ch with sugars A B Fig Close-up of the 1H–15N HSQC regions showing the chemical exchange for Asp18 or Trp33sc with increasing amounts of some sugars Peak movements of main ... structure of EW29Ch with lactose showed that the glucose residue of the lactose molecule interacted with subdomain c of EW29Ch [25] This interaction may be an artifact caused by the crystallization of...

Ngày tải lên: 23/03/2014, 04:21

... either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide dismutase ... minutes of anaerobiosis, small amounts of sodium dithionite solution were added to attain a level of reduction of heme b and hemin of 90–95% Sequential additions of small aliquots (0.2 lL) of a ... presence of a detergent or of a mutation such as Arg54Ser [14], the structural arrangement of the FAD binding domain of flavocytochrome b is modified, resulting in a loss of interaction of Babior,...

Ngày tải lên: 31/03/2014, 21:21

... [17] of patients with RA, but that has not been studied extensively in RA due to controversial data on its role in the pathogenesis of disease [18] The present research on the interaction of RA-IgG ... in vivo model of cartilage destruction Although a number of molecular pathways have been identified that contribute to the stable activation of RASFs, the precise cause and nature of this activation, ... colleagues that in a cohort of patients with recent onset arthritis, patients who later developed RA showed significantly higher serum levels of IL-16 than patients with undifferentiated arthritis...

Ngày tải lên: 09/08/2014, 06:22

... infection, a lag phase of several years is often observed before the appearance of symptoms whose intensity on a given plant may vary with each year However, infected plants die within a few years ... Sauvignon plantlets were experimentally infected with NE85-1 E lata strain in three independent series Seven weeks after infection, the plantlets with confirmed eutypiosis and healthy plantlets ... response of plants towards fungal pathogens In the interaction between Cladosporium fulvum and tomato, resistance against the fungus correlates with early induction of transcription of genes...

Ngày tải lên: 12/08/2014, 03:21

... replacement of G with A leads to a loss of one hydrogen bond along with appositional interactions in the donor atoms of the protein Replacement with C, on the other hand, results in the loss of both ... noncognate (PV1,noncog) complexes within 0.35 nm of the point of substitution (Region R4) and within 0.35 nm of the full recognition site 49 Figure 3-12 Comparison of DNA Structure ... INTERACTION OF ECORI WITH NONCOGNATE DNA SEQUENCES: COMPUTATIONAL INVESTIGATION OF DYNAMICS OF PROTEIN & WATER AND DNA CONFORMATION VIGNESHWAR RAMAKRISHNAN (B Tech., PSG College of Technology,...

Ngày tải lên: 09/09/2015, 18:49

... the direction of movement, with the anterior facing the migration front and set off a series of processes that produces cell motility Foremost of these is the generation of actin mediated lamellipodia ... Co-localization and interaction of ZO-2 and ZO-3 with Scrib in COS-1 Figure 2-3 Scrib interacts directly with ZO-2 and ZO-3 via its PDZ domains Figure 2-4 Co-localization of ZO-2 and ZO-3 with Scrib in ... INTERACTION OF SCRIBBLE WITH ZONULA OCCLUDENS AND INTERMEDIATE FILAMENT PROTEINS DOMINIC PHUA CHENG YANG B.Sc (Hons.), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY...

Ngày tải lên: 14/09/2015, 08:24

... effect of DNA on hydrodynamic radius (Rh) of hRad51 hRad51 (1 lM) was incubated with 0, 1, 3, and lM of oligo PUC+ (in the absence of any nucleotide cofactor) followed by the measurement of hydrodynamic ... B Fig Effect of DNA on the interaction of hRad51–hRad52 (A) hRad52 selectively interacts with higher oligomeric forms of hRad51 in the absence of DNA Rad51 (10 lM) was incubated with 0, 2.5, ... associated with the disappearance of small ones To assess the role of hRad52 on hRad51 binding, we performed gel shift analyses of complexes at a limiting amount of hRad51 (1 lm) in the presence of increasing...

Ngày tải lên: 16/03/2014, 14:20

... detection of proteins/ peptides in most microarray assays The surface of the glass slide is usually derivatized with chemicals to generate different types of molecular layers Immobilization of proteins/ peptides ... interactions of proteins inside living cells is critical for a better understanding of cellular mechanisms and functions Traditionally this has been done by in vitro labeling of proteins with fluorescent ... labeled with biarsenical probes Their utility in live cell imaging was demonstrated in visualizing the translocation of connexin in and out of gap junctions.[25] Non- covalent interactions of small...

Ngày tải lên: 08/11/2015, 16:30

... expression of vpr or HIV infection elicits a transient activation of the small heat shock proteins (sHsps) of eukaryotes through an Hsfmediated pathway Activation of these sHsps is most likely a part of ... inability of Vpr' to compete with Hsf -mediated Hsp16 elevation Thus, assuming that expression of hsp16 is responsive to both the presence of Vpr and heat shock treatment, this larger increase of Hsp16 ... supernatants To neutralize the effect of potential contamination with endotoxin [45], 10 μg/ml of Polymyxin B (PMB) was added [45] Results are mean ± SE of triplicates Page of 14 (page number not for citation...

Ngày tải lên: 13/08/2014, 09:20

... binding of the a-carboxylate and a-amino groups of BEC were also ascribed to the larger volume of the active site cleft of arginase II, which allows more water -mediated enzyme–inhibitor interactions ... the active sites of this enzyme and arginase I In spite of the relatively low agmatinase activity of the Asn149Asp variant, it is clear that the interactions of arginase II with l-arginine and ... blue staining of purified enzymes Metal-free species of purified enzymes were obtained by incubation for h at 25 °C with 25 mm EDTA and m 4545 ´ V Lopez et al Interaction of arginase II with substrate...

Ngày tải lên: 20/02/2014, 02:21

... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the light ... intensity of the band of unhydrolyzed actin was plotted against the time of incubation Characterization of actin preparation quality Fluorescence parameters were used for estimation of nativity of actin ... parameter A of actin was close to 2.4 Increase of the time of heating at 43 °C leading to decrease of parameter A up to 2.2–2.3 was accompanied by further decrease of the initial rate of polymerization...

Ngày tải lên: 20/02/2014, 23:20

... equilibrated with 10 column volumes (10 · 0.2 mL) of buffer B, with subsequent washing four times with five volumes of the same buffer Proteins were eluted with buffer E (buffer B supplemented with 250 ... 410–421 Interaction of TnrA with GlnK and GS Fig S2 TnrA C-terminal truncations Fig S3 Crosslinking analysis of truncated TnrA proteins Doc S1 Purification of His6-tagged TnrA proteins, purification of ... control for nonspecific interactions Figure 2A shows a response difference sensorgram (FC2 – FC1) of interactions of GlnK with immobilized TnrA For this analysis, an analyte concentration of 40 nm...

Ngày tải lên: 06/03/2014, 00:21

... analysis The interaction of soluble b chains with SAgs was monitored in a resonance mirror with an IAsys instrument (Labsystem, Cambridge, UK) biosensor, which allows determination of real time interactions ... lower Kd The Kd calculation is independent of the amount of Ó FEBS 2004 Interaction of human TCR with superantigen SSA (Eur J Biochem 271) 4081 Fig Vb1–SSA interaction analysis Association curves ... the binding of Vb1–SSA with Kd ¼ 10.82 lM (E) and Vb1–SSA(C26S) with a Kd ¼ 9.14 lM (F) Fig SSA dimer in supernatant of S pyogenes Supernatants of isolates from patients infected with S pyogenes...

Ngày tải lên: 07/03/2014, 16:20