nanobody new agent for combating against breast cancer cells

... of triple-negative breast cancer that has a poor prognosis As such, it is often used as xenografts in nude mice to test new drugs and therapeutic strategies against breast cancer Recent studies ... Targeting CD138 for RIT is attractive since this antigen is associated with an aggressive breast cancer phenotype CD138 is expressed by about 75% of ER-negative forms of breast cancer [39], and ... proliferative breast carcinoma subtype Breast Cancer Res Treat 2006, 98(1):91-8 Rakha EA, El-Sayed ME, Green AR, Lee AH, Robertson JF, Ellis IO: Prognostic markers in triple-negative breast cancer Cancer...

Ngày tải lên: 21/06/2014, 01:20

... successive use of newer chemotherapeutic agents for the treatment of breast cancer, it is likely that new prevention agents will improve upon the benefits achieved with tamoxifen The new NSABP chemoprevention ... to treat the unprevented breast cancer Tools that can be used for determining a woman’s breast cancer risk and the net effect from tamoxifen when used to prevent breast cancer are currently being ... Natl Cancer Inst 1991;83: 1299–306 (13) Fisher B, Redmond C New perspective on cancer of the contralateral breast: a marker for assessing tamoxifen as a preventive agent [editorial] J Natl Cancer...

Ngày tải lên: 15/02/2014, 04:20

...  our cells  Normally,  our cells  grow  and   divide  to  form new cells  in  a  natural  renewal  process   When cells  grow  old,  they  die,  and new cells  take  their   place  Sometimes, ...  get breast cancer  This  book  is  about  what  to    before  you  get breast cancer  in  order  to  prevent  it,  or   reduce  risk  of  recurrence     Cancer  begins  in  our cells ...  survived breast cancer  twice  and  is  a  tireless   champion for breast cancer  advocacy  She  is  the   University  Distinguished  Service  Associate  Professor  of   Breast Cancer  and...

Ngày tải lên: 22/03/2014, 16:21

... Adjuvant therapy for node-negative breast cancer Treatment policy 21 Figure Adjuvant therapy for node-positive breast cancer 22 Guidelines for management of breast cancer Definitions for response ... Technical Publications Series ; 31) Breast neoplasms – Therapy Breast neoplasms – Diagnosis Breast – Cancer – Guidelines Breast – Cancer I Title II WHO Regional Office for the Eastern Mediterranean ... that most patients with breast cancer ultimately require treatment for advanced disease Clinical breast cancer research has focused on effective methods to detect breast cancer at its earliest...

Ngày tải lên: 28/03/2014, 23:20

... decisions in axillary node-negative breast cancer Annu Rev Med 1993, 44:201-210 Polychemotherapy for early breast cancer: an overview of the randomised trials Early Breast Cancer Trialists’ Collaborative ... histologic grade for 22,616 cases of breast cancer The basis for a prognostic index Cancer 1991, 68:2142-2149 11 Saez RA, McGuire WL, Clark GM: Prognostic factors in breast cancer Semin Surg ... indicator for node-negative breast cancer [5] These investigations would provide validation of the significance of morphologic complexity of epithelial architecture in node-negative breast cancer, ...

Ngày tải lên: 18/06/2014, 16:20

... receptor positive lymph node negative (ER+LN-) breast cancer Another study validated a predictive score, based on 21 genes, for ER+LN-tamoxifen treated breast cancer [2] These results are encouraging, ... primary breast cancer J Clin Oncol 2006, 24:1665-1671 Fan C, Oh DS, Wessels L, Weigelt B, Nuyten DS, Nobel AB, van't Veer LJ, Perou CM: Concordance among gene-expressionbased predictors for breast cancer ... organization, nucleosome assembly Top ranked 17 prognostic genes in ER+ breast cancer as determined by a meta-analysis of three major breast cancer data sets We give the sign of their global average Cox-regression...

Ngày tải lên: 14/08/2014, 17:22

... to cancer gene therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells ... approximately one in five breast cancers is human epidermal growth factor receptor 2/erb-B2 (HER2)positive breast cancers Hormone receptor-positive and HER2-positive breast cancers have highly effective ... targeted treatments available Triple-negative breast cancer (TNBC) accounts for 15% to 20% of breast cancers (Konecny G 14 et al., 2003) Therapy for TNBC is empiric and not based on tumor biology,...

Ngày tải lên: 09/09/2015, 18:56

... of breast cancer Inflammatory breast cancer (IBC) is the most aggressive form of breast cancer but consists only 1% to 3% of all cases breast cancer According to the receptor status, breast cancer ... of synthesized chalcones 1.2 Breast cancer overview 1.2.1 Breast cancer progression and types Breast cancer is a malignant tumor that starts in the cells of the breast It occurs mostly in women, ... cancer Triple-negative breast cancer refers to any breast cancer that does not express the gene for ER, Her2 and PR Breast cancer progression begins with the normal breast terminal ductal lobular...

Ngày tải lên: 02/10/2015, 17:14

... which in their entirety can be responsible for the constricted sensitivity against alkylating agents It is clear that effort is necessary to search for new forms of treatment modalities like drug ... 4-diamidino-2-phenylindole (DAPI) for staining the cell suspension was performed Each histogram represents 30.000 cells for measuring DNA-index and cell cycle For histogram analysis we used the ... aneuploid DU 145 prostate cancer cells after treatment with TMZ alone and with its TMZ-BioShuttle derivative Control DU 145 cells form a continuous monolayer, while treated DU 145 cells show loss of...

Ngày tải lên: 26/10/2012, 09:48

... ¼ 10 for control or DENSPM-treated cells; n ¼ for Z-VAD.FMK treatment; n ¼ for DENSPM + Z-VAD.FMK treatment) and bars represent ± SEM White bars, control cells; black bars, DENSPM-treated cells; ... human breast cancer cells by an unsymmetrically alkylated polyamine analogue Cancer Res 55, 3233–3236 31 Asker, C., Wiman, K.G & Selivanova, G (1999) p53-induced apoptosis as a safeguard against cancer ... property of an anticancer treatment is to minimize the damage to normal cells DENSPM and other polyamine analogues may have different toxic effects on normal cells and cancer cells In the present...

Ngày tải lên: 17/03/2014, 23:20

... MCF7 breast cancer cells engineered to overexpress Her2-18 have a very high number of Her2-binding sites per cell (11.28 × 106), followed by BT-474 breast cancer cells (2.75 × 106), MCF7 breast cancer ... MCF7/Her2-18 Hathaway et al Breast Cancer Research 2011, 13:R108 http:/ /breast- cancer- research.com/content/13/5/R108 Cells only Cells only Isotype Page of 13 Her2 Isotype Her2 Isotype Cells only Her2 A ... Hathaway et al Breast Cancer Research 2011, 13:R108 http:/ /breast- cancer- research.com/content/13/5/R108 Introduction New cases of invasive breast cancer were predicted to exceed...

Ngày tải lên: 22/03/2014, 17:20

... expressed in MPA-treated cells, but not in the control cells at either 24 or 48 h in differential display analysis Progestins upregulate GPR30 mRNA in MCF-7 breast cancer cells Northern-blot analysis ... was made of the gene expression patterns in MCF-7 breast cancer cells cultured in the presence of MPA and estrogen with estrogen-treated control cells by the differential display PCR technique ... SYBR Green I kit (Roche) was used The reverse transcription was performed at 61 °C for 20 min, and the denaturation at 95 °C for Forty cycles of PCR were carried out Each cycle of PCR included denaturation...

Ngày tải lên: 24/03/2014, 00:21

... the cells *P < 0.05; mean ± SE; n ¼ 90 cells Magnification, 400· (B) One microgram of neutralizing antibody against TRAIL was added to MDA-MB-468 cells for h before initiation of hypoxia for 24 ... cells but not in nonmalignant cells MDA-MB-468 cells, MCF-7 cells, MCF-12A cells or HMECs were exposed to normoxia or hypoxia (1% O2) for 24 h prior to (A) immunostaining for BRCA1 (magnification, ... localization and induce apoptosis in breast cancer cells Therefore, we assessed the requirement for functional BRCA1 in TRAIL-stimulated apoptosis HCC1937 cells have a 5382insC mutation in BRCA1,...

Ngày tải lên: 30/03/2014, 03:20

... reduced the OPN mRNA level in MDA-MB-231 breast cancer cells by transfection with OPN specific siRNA Methods Cell culture conditions The human breast cancer cell line MDA-MB-231 was grown as ... housekeeping gene and for control of cDNA integrity PCR conditions were: 95°C for 15 followed by 40 cycles of denaturation for 30 s at 95°C, hybridization for 30 s at 60°C, extension for 30 s at 72°C, ... humidified atmosphere of 3% CO at 37°C The cells were incubated for two weeks and then fixed with paraformaldehyde (Sigma), and colony formation (colonies of ≥50 cells) was visualized by staining with...

Ngày tải lên: 09/08/2014, 09:20

... control cells treated with water for the total number of cells, exclusively viable, and for dead cells after and 50 U/ml for day, days, and days (n = 4-14 wells per group) For counting, cells were ... percentage SA-b-gal-positive cells Total cell numbers per group of 759-963 cells for P1 and 510803 cells for P2 were counted In addition to this, cells from a human breast tumor (MaCa 700) were ... decrease in number of cells was observed for Lewis cells after days and also for F344 cells after and days (Figure 2a) These results were evaluated from the total number of counted cells including...

Ngày tải lên: 10/08/2014, 10:21

... 1.1.2 1.1.3 Breast cancer Incidence of breast cancer Classification of breast cancer Aetiology of breast cancer 1 1.2 1.2.1 1.2.2 1.2.3 1.2.4 1.2.5 1.2.6 HER-2/neu-positive breast cancer Definition ... frequent cancers affecting Singapore women, 2003-2007 Table 1-3 Risk factors for breast cancer Table 1-4 HER-2/neu status and breast pathology Table 1-5 Summary of HER-2/neu tests for breast cancer ... coding for p53 Vascular endothelial growth factor XVI Chapter Introduction CHAPTER INTRODUCTION 1.1 Breast cancer 1.1.1 Incidence of breast cancer Breast cancer is the second leading cause of cancer...

Ngày tải lên: 11/09/2015, 10:06

... the cancer cells in the context of cancer metastasis Specifically this project aims to: Model breast cancer cells traversing blood capillaries during hematogenous metastasis by flowing cancer cells ... cells deformed more than the normal MCF-10 breast epithelial cells 35 Chapter Microfluidics study of breast cancer cells in suspension Lincoln et al had also used the optical stretcher to deform ... Microfluidics study of breast cancer cells in suspension In this chapter, in an attempt to compare the overall deformability of benign and cancerous breast epithelial cells under large deformation, we...

Ngày tải lên: 14/09/2015, 08:48

... breast cancer cells 37 3.1.1 Breast cancer cells are sensitive to the anti-proliferative effects of TQ 37 3.1.2 TQ causes deficiencies in cell cycle checkpoint function in breast cancer cells ... in TQ-treated breast cancer cells 44 3.2 DNA damaging effects of TQ in normal and breast cancer cells 47 3.2.1 TQ induces significantly greater DNA damage in breast cancer cells 47 3.2.2 ... of breast cancer cells 60 3.4.2 Telomere shortening in breast cancer cells at weeks of TQ treatment 62 3.4.3 Prolonged exposure to TQ alters hTERT and TRF2 expression levels in breast cancer...

Ngày tải lên: 30/09/2015, 13:41

... well spread cells were scanned for each set. The scanning parameters were  30µm×30µm for scan size, 0.2 and 0.4 for PI control, 60µm/s for scan rate, 1nN for scan force, and 90⁰ for scan direction. All the imaging was performed over the center  ... The representative result is shown in Fig. 9. The scan parameters are 30µm×30µm  for scan size, 0.2 and 0.4 for PI control, 60µm/s for scan rate, 1nN for scan force, 90⁰  for scan direction, and 20nm for tip radius. The images were acquired on the center  ... an optimized imaging force for different cells which can show the filamentous  network clearly and cause little damage to the cells.  Here, 1nN was found as a  moderate imaging force for living MCF‐7 cells,  which led to a 300nm deformation of ...

Ngày tải lên: 30/09/2015, 14:23