ms using ion trap instruments

... 20% glycerol The reactions were carried out at 30 °C for 10 After the incubation, samples were immediately subjected to tryptic digestion and analysed using LC ⁄ MS Purification of commercial methylmalonyl-CoA ... scanning from m ⁄ z 50 to 200; and MS ⁄ MS mode with m ⁄ z 147.1 as the precursor ion and collision energy at 20.5% Fractions containing the hydrazide reaction product were also collected, lyophilized ... fragments, fractionate the complex mixture and use high-resolution electrospray ionization Fourier-Transform mass spectrometry Changing patterns of covalent active-site occupancy (ESI ⁄ FTMS) to pick...

Ngày tải lên: 16/03/2014, 03:20

... pulse.) We take the photoionization cross-section from the 2p0 state to be ␴ionize Ϸ ϫ 10Ϫ21 m2, calculated by using the hydrogenic 2p continuum photoionization cross-section (21), appropriately ... SCIENCES Multiphoton Ionization If we take the absorption cross-section for 1s(A1) 2p0 to be ␴abs ϳ ϫ 10Ϫ18 m2 from the small-signal absorption spectrum, and note that the Si transmission coefficient ... recombination time 1/Ptot Ϸ 16 ps, which sets the time scale for recombination under complete ionization, can be found by taking Brown’s value for the electron recombination cross-section ␴recom...

Ngày tải lên: 16/03/2014, 15:30

... concentration of 52 lm The reaction mixture was incubated at 30 °C for min, and a portion was then analyzed by LC -MS To the rest of the reaction mixture propionyl-CoA was added to a final concentration ... in combination with on-line liquid chromatography ion trap mass spectrometry studies on the multifunctional proteins, DEBS1-TE, DEBS3 and DKS as model systems, introduces an additional powerful ... acylation of DKS (see later), a shorter digestion protocol was also investigated We found that a 5-min digestion using a DKS ⁄ trypsin ratio of 80 : (w ⁄ w) at 30 °C resulted in the same digestion...

Ngày tải lên: 16/03/2014, 18:20

... implantation, the QD GS luminescence peak (P10 ) from the implanted region is blue shifted with respect to peak P1 from the un-implanted region Under the implantation and annealing conditions used ... region of the device structures annealed with and without implantation For the un-implanted region, the QD ground state (GS) luminescence peaks at 1015 nm (P1) Due to enhanced interdiffusion ... respectively Thermal population of QD ES depends on the energy separation between the GS and ES (DE in Fig 1) The separation between consecutive energy levels in the conduction band calculated from...

Ngày tải lên: 22/06/2014, 18:20

... (information carrier) In addition, a cavity also provides other useful functions for a trapped ion system such as ion cooling This thesis explores various functionalities of a trapped ion- cavity ... cavity enhances the interaction between the ion and a single photon, and enables efficient collection of the ion emissions Proposed applications of trapped ion- cavity systems in QIP include quantum ... initial conditions of the ion position and velocity The solutions of Equation 2.7 and Equation 2.8 comprise motions in two timescales, corresponding to the secular motion and the micromotion The secular...

Ngày tải lên: 10/09/2015, 09:05

... ‘Close’ option The Navigation Pane The navigation pane replaces the database window used in previous versions of MS Access It holds all objects in the database such as tables, forms, queries, ... in the forms section, click the ‘Form’ option 12 MS Access creates a quick form The form contains the columns and data you created in the table At the bottom of the form are the navigation buttons ... database Load up MS Access 2007 and the opening screen will appear Click the option ‘New Blank Database’ You can also click the MS Office button at the top left Then select the option called ‘New’...

Ngày tải lên: 24/12/2013, 02:15

... Units Used: kg = 1000 g −3 ms = 10 s kN = 10 N Given: S1 = 8653 ms S2 = 8368 N S3 = 0.893 kg Solution: ( a) S1 = 8.653 s © 2007 R C Hibbeler Published by Pearson Education, Inc., Upper Saddle River, ... in SI units using an appropriate prefix: (a) a 1/b1, (b) a2b2/c2, (c) a3b3 Units Used: μm = 10 −6 m Mm = 10 m Mg = 10 gm kg = 10 gm −3 ms = 10 s Given: a1 = 684 μm b1 = 43 ms a2 = 28 ms b2 = 0.0458 ... in the correct SI form: (a) Mg /ms, (b) N/mm, (c) mN/( kg⋅ μs) Solution: ( a) Mg 10 kg 10 kg Gg = = = −3 ms s s 10 s Mg Gg = ms s N = mm ( b) 1N 10 −3 = 10 3N m m = kN m N kN = mm m mN ( c) kg⋅...

Ngày tải lên: 17/02/2014, 14:20

... for the rapid detection of hydrophobins from a large number of small samples, we have investigated the potential of intact-cell MALDI-TOF MS (ICMS) Initial applications of ICMS on filamentous fungi ... reaction is the cleavage of C-terminal peptides at Ala sites, e.g at sites + from the last conserved Cys, at Lys-Ala positions, at Glu-Ala positions, or at Thr-Ala positions These predictions ... Characterization of intact Penicillium spores by matrix-assisted laser desorption ⁄ ionization mass spectrometry Rapid Commun Mass Spectrom 19, 3564–3568 10 Li TY, Liu BH & Chen YC (2000) Characterization...

Ngày tải lên: 19/02/2014, 02:20

... glucose, and specific glucose consumption rate, using a predetermined correlation factor of 0.44 g cellular dry weight (CDW) per D600 unit Sample preparation and GC -MS measurements Aliquots of batch ... )70 eV GC -MS raw data were analyzed using the software package MassLab (Fisons), avoiding detector overload and isotope fractionation as described [20] The amino acids analyzed by GC -MS were aspartate, ... fractional enrichment of C2 and C3 in malate does not differ from the fractional enrichment in the input substrate A dilution of the fractional enrichment might be observed, for example, in positions...

Ngày tải lên: 20/02/2014, 23:20

... waters Number of inhibitor atoms Baverage (A2) Protein main chain atoms Protein side chain atoms All protein atoms Inhibitor atoms Water atoms Other atoms All atoms PDB code Methyl 4,4II,4III,4IVtetrathio-a-D-xylopentaoside ... Number of observations Number of unique reections Temperature (K) Rfactor (%) Rfree (%) Rmsd bond lengths (A) Rmsd bond angles () Number of all atoms Number of protein atoms Number of waters ... Quantitative determination of noncovalent binding interactions using soft ionization mass spectrometry Int J Mass Spectrom 216, 127 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein...

Ngày tải lên: 07/03/2014, 17:20

... Organization Participation related terms ▪ participation ▪ handicap ▪ patient participation ▪ consumer participation ▪ community re-integration ▪ community integration ▪ social adaptation ▪ social ... manuscript Appendix List of search terms Conceptual model terms ▪ International Classification of Functioning, Disability and Health (ICF) ▪ International Classification of Impairment, Disability and ... life situations that comprise participation Participation is defined in the International Classification of Functioning, Disability and Health (ICF) as the 'involvement in a life situation' and...

Ngày tải lên: 18/06/2014, 19:20

... the conventional MFI zeolite Figure exhibits the nitrogen adsorption-desorption isotherms and pore size distributions of the investigated catalysts Both catalysts showed type IV isotherms in accordance ... adsorption gas at the temperature of liquid nitrogen The Page of nitrogen adsorption-desorption isotherms and BET surface area were then obtained The surface acidity of the catalysts was measured using ... hexagonal material with an increase in adsorption in the range of P/P = Figure Nitrogen adsorption-desorption isotherms (a) and pore size distributions (b) of nanoporous catalysts Lee et al Nanoscale...

Ngày tải lên: 21/06/2014, 01:20

... create a symbolic representation of a sound scene in terms of individual sources, CASA heuristics can be used within source separation algorithms to both identify mixture regions in which source signals ... The remainder of this section describes the implementation of the proposed source separation algorithm in greater detail 3.2 Mixing parameter estimation In this section, we give a brief overview ... Stage three reconstruction (2) Multi-source reconstruction (3) Residual reconstruction Final source estimates Reconstructed source waveforms (f) (g) Figure 2: An illustration of the three stages...

Ngày tải lên: 22/06/2014, 23:20

... Several potentiometric studies using chitosan as membrane for ion- selective electrodes were reported.3,6 The previous study9 on the determination of Fe3+ ions using a heterogeneous chitosan membrane ... solutions was adjusted with a conventional glass electrode No 91-02 (Orion, USA) A commercial chloride electrode model 94-17B (Orion, USA) was used as comparison The samples were stirred using ... Cl– Membrane surface | Cl– solution | Figure 2: Illustration of ion- exchange mechanism at the surface of membrane The mobility to and exchange of Cl– ion at cationic sites in the chitosan skeletons...

Ngày tải lên: 07/08/2014, 14:20

... the summation Purification An item with DIF might bias the scale score estimate, making it less valid as a matching criterion for other items Some DIF studies have employed “purification” [35], ... incorporating purification by using an iterative approach that can account for DIF in other items [63,64] It is our view, however, that the standard logistic regression approach using sum scores is ... than ten items [3-5,7-9,11,19,20, 22,24,61] Another common problem with HRQoL instruments is items with floor and ceiling effects, or with highly skewed score distributions These items will not...

Ngày tải lên: 12/08/2014, 01:21

... were semi-quantified using substitutive Table The identification results of saponins in the XST injection by LC/MSn Peak No Identification Retention time (min) MS[ M-H]- MS data (m/z) Notoginsenoside ... Additional file 2: The similarities of chromatograms of 10 samples (n = 3) Additional file 3: PDA Chromatograms standard compounds (A) and a XST injection (C), and total ion current chromatograms ... Full scan data acquisition was performed from m/z 80 to 1800 in MS scan mode The MSn spectra were obtained with the collision energy for collision- Page of induced dissociation adjusted to 30%-40%...

Ngày tải lên: 13/08/2014, 14:20

... coupled with an ion trap mass spectrometry detector was used The system was equipped with an electrospray ionization (ESI) source and an ion trap analyzer for UV and MS data acquisition A reverse ... resolution and peak symmetry values were determined as the indicators of separation efficiency Resolution values were all higher than 1.5, which indicates good separation Six replicate injections ... cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI -MS/ MS) Anal Chim Acta 2006, 567(2):218-228 28...

Ngày tải lên: 13/08/2014, 15:21

... sphere DISTRIBUTUION OF THE LASER INTENSITY AND THE FORCE ACTING ON 249 IV CONCLUSION In conclusion, we find that the total intensity and total optical forces in 3D -trap using counter-propagating ... hollow Gaussian beams”, Phys Lett A (2006) 502-506 [3] Ho Quang Quy, Mai Van Luu, “Radiation Force Distribution of Optical Trapping by Two Counterpropagating CW Gaussian Beams Acting on Rayleigh ... nanoparticle in optical trap using counter-propagating pulsed laser Beams”, Chinese Optical Letters (2010) 332-334 [6] A A Ambardekar, Y Q Li, “Optical levitation and manipulation of stuck particles...

Ngày tải lên: 30/10/2015, 20:51

... ES-1232 evaluation board from Softbaugh, equipped with an MSP430F1232 MCU from Texas Instruments The code was debugged using the USB debug interface, MSP-FET430UIF, available from Texas Instruments ... to the Section “Initialization”) DS01234A-page © 2008 Microchip Technology Inc AN1234 INITIALIZATION The ini_spi routine prepares the MCU for communication with the serial EEPROM, using the hardware ... through the MSP430 USB debug interface, MSP-FET430UIF, from Texas Instruments The code was tested using the 25AA080A serial EEPROM Oscilloscope screen shots are shown in this application note All...

Ngày tải lên: 11/01/2016, 17:02

... phí để hồn thành dự án theo kế hoạch(Budged At Completion BAC) ƒEAC= Chi phí ước tính để hồn thành dự án theo thực tế (Estimated At Completion - EAC) ƒCV= Chênh lệch chi phí (Cost Variance – CV) ... EAC (Estimate at completion) để hoàn thành hạng mục công trình tổng chi phí thực tế (ACWP) + chi phí ước tính để hoàn thành phần việc lại (ETC Estimated Cost to Completion) EAC=ETC+ ACWP EAC= ... Trong đó: + EAC ( Estimate at completion) thể chi phí cuối để hoàn thành công việc Chúng ta để giá trò cho Microsoft tự tính toán + BAC ( Budget at completion) Thể tổng chi phí công tác theo kế...

Ngày tải lên: 06/06/2016, 21:18