ms and lc nmr

... SFE -NMR / 147 6.7 CE -NMR / 147 ix 95 131 143 x CONTENTS 6.8 CEC -NMR / 149 6.9 CZE -NMR / 150 6.10 cITP -NMR / 150 6.11 CapLC -NMR / 152 6.12 SPE -NMR / 154 6.13 SPE -MS- NMR / 159 6.14 Conclusions and ... capillary HPLC (capLC) as capLCNMR and solid-phase extraction (SPE) as SPE -NMR, including SPE hyphenated to MS- NMR as SPE -MS- NMR Many laboratories in industry and academia have NMR as a hyphenated ... Hyphenated with NMR / 49 2.5 Current Trends / 52 References / 52 Basic Technical Aspects and Operation of LC- NMR and LC -MS- NMR 3.1 Introduction / 59 3.2 Technical Considerations Regarding LC- NMR / 59...

Ngày tải lên: 05/03/2014, 10:20

... [13,14] is unique to 3-linked GlcNAc and Glc residues, whereas 0,2A-type cleavages [15] only occur with 4-linked GlcNAc and Glc, resulting in fragment ions, indicating type and type chains or blood ... °CÆmin)1 and to 310 °C at 10 °CÆmin)1 ES -MS Negative-ion ES -MS and CID MS/ MS were carried out on a Micromass (Manchester, UK) Q-Tof mass spectrometer Nitrogen was used as desolvation and nebuliser ... optimal fragmentation and, typically, 40–43 V was used for CID of [M-H]–, and 23–27 V for [M-H]2– A scan rate of 1.5 s per scan was used for both ES -MS and CID MS/ MS experiments, and the acquired...

Ngày tải lên: 19/02/2014, 12:20

... template and the oligonucleotides FAD(CA)-1 and FAD(CA)-2 as primers (Table 1) The 1006-bp amplication product was digested with EcoRI and BamHI and ligated into the vector pMal-c2 (New England Biolabs) ... 31P -NMR spectrum of wild-type TrxR in the oxidized (A) and the reduced state (B) Table 31P -NMR chemical shifts (in p.p.m.) of native and native reconstituted thioredoxin reductase (TrxR), and ... interpretation and the assignment of the chemical shifts are based on published 13C- and 15N -NMR studies on proteinbound and free avins [17], the data referring to free avins are also given in Tables and...

Ngày tải lên: 16/03/2014, 16:20

... (242) shows that unlike DMSO adduct (298)- relative abundance of Na+ adduct increases with cone voltage (note DMSO adduct at 20V) 20V 65V 157 common DMSO solvent ion 2(DMSO) H+ from sample solvent ... functionalities (e.g., phenols and carboxylic acids) tend to form Na+adducts, and the relative intensities tend to increase with cone voltage DMSO adduct is generally fairly weak and can be driven off ... 157 common DMSO solvent ion 2(DMSO) H+ from sample solvent PrednisoneMH+(359) and D MSO ( MH +78)+ adduct (438) at 20V and 65V shows that higher voltage drives off this adduct (MW 358, MH+ 359)...

Ngày tải lên: 24/01/2014, 04:20

... by standard procedure [7] and monitored by GLC -MS of the partially methylated alditol acetates NMR spectroscopy Chemical shifts obtained by NMR spectroscopy were assigned using 2D homo- and heteronuclear ... constant H1 to H2, between 0.5 and Hz, and the presence of the nuclear Compositional, size of ring and linkage analysis GLC -MS analysis of the acetylated O-methyl glycosides and of the acetylated (S)-2-octyl ... injected from a sample solution earlier analyzed with NMR and five fractions were manually collected and freeze-dried MALDI-TOF MS Fractions and from partial hydrolysis of the O-specific polysaccharide...

Ngày tải lên: 31/03/2014, 09:20

... salmon and shrimp by LC- MS/ MS, in press, Anal Chim Acta Andersen WC, Turnipseed SB, Karbiwnyk CM, Clark, SB, Madson MR, Gieseker CM, Miller RA, Rummel NG, Reimschuessel R Determination and Confirmation ... 10 Calculations Internal Standard Calculation Example Calculation Item Sample Area Internal Standard Area b (intercept) m (slope) µg ISTD added to sample sample weight µg ISTD added to standards ... procedure involves the determination of melamine and cyanuric acid by LC -MS/ MS in animal tissue and infant formula using isotopically labeled internal standards for each compound in order to correct...

Ngày tải lên: 06/10/2016, 09:29

... structure and sample distribution The classification tree using the combination of the four peaks identified 98 CRC and 80 healthy controls with a calculated sensitivity of 92.85% and a specificity ... immunologic and biochemistry test But, the sensitivity and specificity of the current biomarkers in tumor diagnosis is low (usually less than 70%) and complicated by high return of ‘false-positives’ and ... and had the informed consent of the patients and volunteers The patients and serum samples were then divided into two groups: the ‘‘training’’ set and the blinded ‘‘test’’ set (Table 2).The blood...

Ngày tải lên: 25/10/2012, 11:18

... 2.3.1.1 Thiết bị  Hệ thống sắc ký lỏng khối phổ khối phổ LC /MS/ MS Thermo bao gồm: phận bơm dung môi, loại khí, phận điều nhiệt detector MS  Cột sắc ký C18 Water (150mm × 4,6mm × 5μm)  Máy lắc ... 15.90 NL: 1.07E4 TIC F: + c ESI SRM ms2 202.00@45.00 [ 17.48 144.50-145.50] MS mix 5ppb NL: 7.56E3 TIC F: + c ESI SRM ms2 208.00@37.00 [ 17.15 17.59 151.50-152.50] MS mix 5ppb 0 10 Time (min) 12 14 ... cất - Kênh B: methanol 30 Chương KẾT QUẢ VÀ THẢO LUẬN 3.1 Tối ưu điều kiện xác định carbamat LC /MS/ MS 3.1.1 Chọn điều kiện chạy detector khối phổ 3.1.1.1 Khảo sát điều kiện bắn phá ion mẹ Các...

Ngày tải lên: 31/10/2012, 08:48

... 2.3.1.1 Thiết bị  Hệ thống sắc ký lỏng khối phổ khối phổ LC /MS/ MS Thermo bao gồm: phận bơm dung môi, loại khí, phận điều nhiệt detector MS  Cột sắc ký C18 Water (150mm × 4,6mm × 5μm)  Máy lắc ... 15.90 NL: 1.07E4 TIC F: + c ESI SRM ms2 202.00@45.00 [ 17.48 144.50-145.50] MS mix 5ppb NL: 7.56E3 TIC F: + c ESI SRM ms2 208.00@37.00 [ 17.15 17.59 151.50-152.50] MS mix 5ppb 0 10 Time (min) 12 14 ... lienhe@docs.vn Tel (: 0918.775.368 Chương KẾT QUẢ VÀ THẢO LUẬN 3.1 Tối ưu điều kiện xác định carbamat LC /MS/ MS 3.1.1 Chọn điều kiện chạy detector khối phổ 3.1.1.1 Khảo sát điều kiện bắn phá ion mẹ Các...

Ngày tải lên: 18/03/2013, 11:04

... chuột 10 1.4.2 Phương pháp sắc ký lỏng (LC- UV, LC- DAD, LC- FLD, LC -MS/ MS) 11 1.5 Ưu nhược điểm phương pháp dẫn đến việc sử dụng phương pháp LC -MS/ MS phân tích DA 12 1.5.1 Phương pháp ... diễn đường tuyến tính .48 Hình 28 Kết chạy mẫu nghêu Nam Định LC -MS/ MS HPLC-UV 53 Hình 29 Kết chạy mẫu CRM LC -MS/ MS HPLC-UV 54 v MỞ ĐẦU Theo ước tính Tổ chức nông lương (FAO), tổng kim ... ký lỏng (LC- UV, LC- DAD, LC- FLD, LCMS /MS) Thường mẫu ly trích dung môi hữu làm cách cho qua cột chiết pha rắn (nếu cần) Sau đó, dịch chiết làm hòa tan dịch pha động tiêm vào hệ thống HPLC với đầu...

Ngày tải lên: 16/04/2013, 19:56

... 2.3.1.1 Thiết bị  Hệ thống sắc ký lỏng khối phổ khối phổ LC /MS/ MS Thermo bao gồm: phận bơm dung môi, loại khí, phận điều nhiệt detector MS  Cột sắc ký C18 Water (150mm × 4,6mm × 5μm)  Máy lắc ... cứu phương pháp xác định đồng thời chất carbamat rau phương pháp sắc ký lỏng khối-phổ khối phổ LC /MS/ MS 2.1.2 Nội dung nghiên cứu 19 Để đạt mục tiêu đề ra, cần nghiên cứu cách có hệ thống vấn đề ... cất - Kênh B: methanol 30 Chương KẾT QUẢ VÀ THẢO LUẬN 3.1 Tối ưu điều kiện xác định carbamat LC /MS/ MS 3.1.1 Chọn điều kiện chạy detector khối phổ 3.1.1.1 Khảo sát điều kiện bắn phá ion mẹ Các...

Ngày tải lên: 04/08/2013, 16:24

... inhabitation of aquatic organisms The results of the measurement and the calculation of DVi, DIi and DI are shown in Table Three chemicals, terbucarb (MBPMC), bromobutide and tolclofos-methyl, were detected ... preparation of samples for ecotoxicity test, and finally acetone solution was analyzed by GC /MS RESULTS AND DISCUSSION Ecotoxicity Level of River Water Histograms of each ecotoxicity effect ratio (growth ... urban area in Japan and agricultural drains when agricultural chemicals are applied In addition, analysis of agricultural chemicals by GC /MS was simultaneously carried out and their ecotoxicity...

Ngày tải lên: 05/09/2013, 10:15

... Server Standard Edition The standard edition provides enterprise-class firewall security and Web caching capabilities for small business, workgroups and departmental environments The standard edition ... location, open a command prompt window, and then run the ISAautorun.exe file Select Install ISA Server from the Microsoft ISA Server Setup window, and then click Continue Type the CD Key, and then click ... Installing and Configuring Firewall Clients ! Troubleshooting Client Installation Problems Before you install and configure ISA Server clients, evaluate the needs of your organization and compare...

Ngày tải lên: 17/01/2014, 09:20

... Appendix D: Authentication in CHAP, MS- CHAP, and MS- CHAP v2 MS- CHAP v2 MS- CHAP v2 authentication is an exchange of four steps: The remote access server sends an MS- CHAP v2 Challenge message to the ... States and/ or other countries The names of actual companies and products mentioned herein may be the trademarks of their respective owners Appendix D: Authentication in CHAP, MS- CHAP, and MS- CHAP ... the hash and compares it to the hash in the MS- CHAP Response If the hashes are the same, the remote access server sends back an MS- CHAP Success message If the hashes are different, an MS- CHAP...

Ngày tải lên: 18/01/2014, 05:20

... Depletion of four nitrofuran antibiotics and their tissue-bound metabolites in porcine tissues and determination using LC -MS/ MS and HPLC-UV Food Additives and Contaminants, pp 405-414 22 Lech Rodziewicz ... sống kỹ thuật sắc ký lỏng khối phổ LC /MS/ MS Các mục tiêu cụ thể sau: - Xây dựng phương pháp xác định đồng thời chất chuyển hóa nitrofuran thực phẩm tươi sống LC -MS/ MS - Ứng dụng phương pháp để xác ... Ionization-Tandem Mass Spectrometry”, Journal of Food and Drug Analysis, Vol 18, No 2, pp 98-106 17 D Tyler (2009), Analysis of nitrofurans in animal tissues a food of animal origin by LC /MS/ MS, sop...

Ngày tải lên: 10/02/2014, 20:55

... pulsed field gradient probe All NMR spectra were acquired at 303 K NMR data were processed using nmrpipe ⁄ nmrdraw [53], and assigned using ansig [54,55] The NMR and MS facilities at the Indian Institute ... carbon and oxygen atoms of pantoate are shown in yellow and red, respectively The carbon and oxygen atoms of ATP are shown in red, the nitrogen atoms in blue, and the phosphorus atoms in orange (see ... chain atoms of Gln61, i.e Oe1 and Ne2, are hydrogen-bonded to the O3 and O4 atoms of pantoate, respectively, whereas the similar atoms of Gln155 are hydrogenbonded to the O2 and O3 atoms of pantoate,...

Ngày tải lên: 16/02/2014, 09:20

... mode and cycled through acquisition of a full mass spectrum and ETD ⁄ PTR MS ⁄ MS spectra on the six most abundant ions every s (400–500 ms per spectrum) Throughout the automated LC -MS ⁄ MS experiment, ... was analyzed by on-line LCMS ⁄ MS The ETD-enabled LTQ instrument was operated in the data-dependent mode and cycled through acquisition of a full mass spectrum and ETD MS ⁄ MS spectra on the six ... reactions and tandem MS ETD has most recently been utilized for the direct analysis of intact proteins, and ubiquitin is a model protein that was initially interrogated via ETD for tandem MS Shown...

Ngày tải lên: 18/02/2014, 16:20

... the zero, xN and 0.87xH frequencies, versus the sequence of the TTF-1 HD Measurements were done at 11.7 T and 286 K Blank slots are for residues 29 and 42 (prolines) and Glu17, Leu34 and Lys46, ... (9.84 ± 0.20 ns) and two for sgi (0.26 ± 0.03 ns and 0.55 ± 0.06 ns) (supplementary Doc S1 and Fig S2) Comparison of results from MF and RSDM The results for sm obtained by the MF and RSDM approaches ... 0.8 mm NMR measurements The 2D NMR spectra were recorded at 286 ± 0.5 K and at 11.7 T on a Bruker (Karlsruhe, Germany) Avance500 spectrometer, operating at 500.13 MHz and 50.68 MHz for H and 15N,...

Ngày tải lên: 18/02/2014, 16:20

... OHMOH (C2) Glc a NHMOH (C3) Glc a Arom ringMGlc b Arom ringMGlc c Arom ringMGlc d 1.9 COO)MOH (C6) Glc b 2.3 COO)MOH (C6) Glc e 2.3 NH2MOH (C2) Glc c NH2MOH (C3) Glc c 1.9 1.9 NH2MH (C2) Glc d 3.0 ... COO)MOH (C6) Glc b COO)MH (C3) Glc a COO)MOH (C3) Glc a NH2MOH (C2) Glc c NH2MOH (C3) Glc c NH2MOH (C6) Glc d COO)MOH (C6) Glc d 3.0 2.3 2.2 1.9 2.0 2.8 1.9 COO)MOH (C1) Glc a COO)MOH (C2) Glc a OHMOH ... 3.0 COO)MOH (C6) Glc d 2.9 2.7 2.5 2.3 2.7 COO)MOH (C3) Glc a COO)MOH (2) Glc a OHMOH (C3) Glc a 2.7 2.1 2.5 COO)MH (C1) Glc c COO)MH (C5) Glc c COO)MOH (C2) Glc f COO)MOH (C3) Glc f 2.9 2.9 2.4...

Ngày tải lên: 18/02/2014, 17:20

... rate constants are similar between NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional ... lM SaDHNA at 25 °C and quenched with M HCl The reverse reaction generated both DHNP and DHMP HP, DHNP, and DHMP were oxidized to HPO, NP, and MP, respectively, before the HPLC analysis as described ... using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA For the forward reaction, three concentrations each for DHNP and DHMP were used...

Ngày tải lên: 19/02/2014, 00:20