h1 nmr and p31 nmr

... of histone H1 have been identified The genes encoding H1. 1, H1. 2, H1. 3, H1. 4, H1. 5 and H1. t are located on the short arm of chromosome 6, while H1 is located on chromosome 22 [4] and H1oo on chromosome ... in codon 18 of H1. 2 and in codon 174 of H1. 4 To obtain the frequency of the two polymorphisms in H1. 2 and H1. 4 in a normal popula- 3680 A 183 bp fragment of the H1. 2 gene (HIST1H1C, GenBank X57129) ... histone H1. 4 in Raji cells By using HILIC, histone H1. 4 was separated into two peaks: one wild-type H1. 4; and one Lys173Arg H1. 4 This microsequence variant was found for the first time and was...

Ngày tải lên: 30/03/2014, 20:20

... pulsed field gradient probe All NMR spectra were acquired at 303 K NMR data were processed using nmrpipe ⁄ nmrdraw [53], and assigned using ansig [54,55] The NMR and MS facilities at the Indian ... Protein–ligand and water-mediated protein–ligand hydrogen bond interactions (broken lines) that stablize the pantoate molecules in site I and site II of nPS Oxygen atoms of pantoate and water ... density maps and facilitate further rebuilding and improvement of the molecular model, until no unexplained electron density remained, and the Rcryst and Rfree values converged at 18.9% and 24.4%,...

Ngày tải lên: 16/02/2014, 09:20

... the zero, xN and 0.87xH frequencies, versus the sequence of the TTF-1 HD Measurements were done at 11.7 T and 286 K Blank slots are for residues 29 and 42 (prolines) and Glu17, Leu34 and Lys46, ... (9.84 ± 0.20 ns) and two for sgi (0.26 ± 0.03 ns and 0.55 ± 0.06 ns) (supplementary Doc S1 and Fig S2) Comparison of results from MF and RSDM The results for sm obtained by the MF and RSDM approaches ... 0.8 mm NMR measurements The 2D NMR spectra were recorded at 286 ± 0.5 K and at 11.7 T on a Bruker (Karlsruhe, Germany) Avance500 spectrometer, operating at 500.13 MHz and 50.68 MHz for H and 15N,...

Ngày tải lên: 18/02/2014, 16:20

... interactions that define the ligand specificity in cellulosomal CBMs and the mechanism by which they recognize and select their substrates, we used X-ray crystallography, NMR and computational chemistry ... complex is formed The ligands cellobiose, cellotetraose and cellohexaose were studied Results and Discussion The crystal structure of CtCBM11, the binding cleft and its ligand specificity In a previous ... six-stranded anti-parallel b-sheets that form a convex side (b-strands 1, 3, 4, 6, and 12) and a concave side (b-strands 2, 5, 7, 8, 10 and 11) The concave side is decorated by the side chains of several...

Ngày tải lên: 18/02/2014, 17:20

... rate constants are similar between NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional ... HP and 20 mM GA The reaction was initiated with 10 lM SaDHNA at 25 °C and quenched with M HCl The reverse reaction generated both DHNP and DHMP HP, DHNP, and DHMP were oxidized to HPO, NP, and ... using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA For the forward reaction, three concentrations each for DHNP and DHMP were used...

Ngày tải lên: 19/02/2014, 00:20

... between Gal II H1 and Glc I H4 in the b-anomer Gal II is a branching point and substituted at positions and 6, as GlcNAc III H1 gives an rOe to H6b of this residue, while GlcNAc VII H1 gives an ... Gal VIII and Fuc XI residues [19,20] The GlcNAc III on the 6-branch is substituted at positions and 4, as Gal IV H1 gives an rOe to H4 of GlcNAc III, and Fuc IX H1 gives an rOe to H3 and to the ... VIII H1 gives an rOe to GlcNAc VII H3 The GlcNAc III on the 6-branch is substituted at the 3- and 4-position, as Gal IV H1 gives an rOe to H4 of GlcNAc III, and Fuc IX H1 gives an rOe to H3 and...

Ngày tải lên: 19/02/2014, 12:20

... transport of lipophilic compounds and drugs and of medium and long chain fatty acids; among them, myristic acid is a stereotypic ligand to investigate fatty acid binding and transport properties of ... sites, and at least three further low affinity sites have been envisaged NMR studies on tryptic and peptic fragments of bovine serum albumin have localized two high affinity sites in domain III and ... propionates point toward the interface between domains I and III and are stabilized by salt bridges with His146 and Lys190 residues [6,8] HSA undergoes pH- and allosteric effector-dependent reversible conformational...

Ngày tải lên: 20/02/2014, 02:22

... e.g trNOEs [20], STD NMR [21–23], and SAR by NMR [24] TrNOE spectra can also be used to elucidate the 3D structure of the bound ligand Saturation transfer difference (STD) NMR is a technique that ... simulation and minimization over 200 steps Both the ligand and the binding site were kept flexible during the simulation is shown in Fig A superposition of NMR glycopeptide structure and X-ray ... Pro1-Asp2-Thr3-Arg4-Pro5 [13] and using nona- and 20mer peptides the resulting epitope was identified as Ala20¢-Pro1-Asp2-Thr3-Arg4-Pro5 and Pro1Asp2-Thr3, respectively [14,15] For SM3 reacting with pentamers and dimers...

Ngày tải lên: 21/02/2014, 15:20

... SFE -NMR / 147 6.7 CE -NMR / 147 ix 95 131 143 x CONTENTS 6.8 CEC -NMR / 149 6.9 CZE -NMR / 150 6.10 cITP -NMR / 150 6.11 CapLC -NMR / 152 6.12 SPE -NMR / 154 6.13 SPE-MS -NMR / 159 6.14 Conclusions and ... capillary HPLC (capLC) as capLCNMR and solid-phase extraction (SPE) as SPE -NMR, including SPE hyphenated to MS -NMR as SPE-MS -NMR Many laboratories in industry and academia have NMR as a hyphenated technique ... show the two- and three-bond CÀH correlations for the protons and carbons numbered in the figure and explained in the text The horizontal and vertical traces are the 1D H and 13 C NMR and spectra...

Ngày tải lên: 05/03/2014, 10:20

... three antiparallel strands (1, and 7) and strand 6, which is parallel to strand b-Strands are located between the four a-helices (a1, 278–294; a2, 305–313; a4, 374–381; and a5, 397–405), with ... data obtained at 288 and 313 K (Table 1) This work made use of the standard double-resonance 15N,1H -NMR and triple-resonance 15N,13C,1H -NMR experiments applied to 13C-labeled and ⁄ or 13N-labeled ... structural elements: b-strands (b3, 329–335; b4, 339–344; and b5, 367–372) and a distorted a-helix (a3, 348–356) (Fig 3B,C) The three b-strands of the minidomain are all antiparallel, and form a single...

Ngày tải lên: 06/03/2014, 11:20

... Cd1H3 Tyr161 Hd a Compound H1 and CH3 H6 ⁄ H6¢ H6 ⁄ H6¢ H6 ⁄ H6¢ H6 ⁄ H6¢ H6 ⁄ H6¢ H1 H1, H2, H5 ⁄ H5¢ H1, H2, H3, H4 H1 and CH3 H1 and CH3 H1 and CH3 H1 and CH3 H1 and CH3 NOEs identified in Fig ... cross-peak between H3 and H6 or H6¢ is assigned as well as the intramolecular NOE between H3 and H1 Table Intermolecular NOEs between West Nile virus (WNV) NS2B–NS3pro(K96A) and inhibitors and Protons ... NS2B and NS3 following Asp90 in the crystal structure with BPTI [4] and was confirmed by narrow NMR line shapes The resonances of the complex with were assigned using NS2B–NS3pro, whereas the 3D NMR...

Ngày tải lên: 07/03/2014, 02:20

... 25 NMR structures was determined on the basis of 2338 experimental restraints measured at 278 K and 298 K (Tables 1–3) This work made use of standard double-resonance and triple-resonance NMR ... 0.4 A In the Ramachandran plot analysis, 89.9% of the residues in the whole NMR family were found in the most favored regions and none in the disallowed regions NMR structure and dynamics of eRF1 ... correlation time and an order NMR structure and dynamics of eRF1 middle domain parameter between 0.5 and 1.0, against a background of faster motions occurring with a correlation time below 20 ns and an...

Ngày tải lên: 07/03/2014, 05:20

... interconversion between Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] ... interfere with the subsequent NMR analysis The NMR resonances of [15N]-amino groups, for example, are at a different chemical shift than the protein amide resonances and therefore not interfere ... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high-throughput production of proteins and selective...

Ngày tải lên: 07/03/2014, 12:20

... ionization) and amino-acid analysis [29] NMR spectroscopy Preliminary NMR spectra were acquired at 400 MHz using a Bruker AMX-400 spectrometer (NMR Centre, University of Ioannina, Greece) High-field NMR ... Agonist H2O Lowest energy NMR structure in H2O Lowest energy NMR structure in H2O Lowest energy NMR structure in Me2SO Lowest energy NMR structure in Me2SO Lowest energy NMR structure in Me2SO in ... various systems comprised 3899 and 4516 SPC molecules for the agonist and the antagonist in water, respectively, and 817 and 927 Me2SO molecules for the agonist and the antagonist, respectively,...

Ngày tải lên: 07/03/2014, 16:20

... Proton CS060606 (p.p.m.) DS040404 (p.p.m.) DUA (F) H1 H2 H3 H4 H1 H2 H3 H4 H5 H6 H6¢ NAc H1 H2 H3 H4 H5 H1 H2 H3 H4 H5 H6 H6¢ NAc H1 H2 H3 H4 H5 H1 H1¢ H2 H3 H4 H5 H6 H6¢ NAc 5.193* 3.796* 4.108* ... assignments of 1H and 13C NMR spectra from a series of disaccharides and tetrasaccharides derived from CS ⁄ DS chains have already been given [16] In this report we present H -NMR and some 13C -NMR data ... Nieduszynski IA, Anderson K, Boocock J, Sandall PL & Weeks SD (2001) Characterization of oligosaccharides from the chondroitin sulfates 1H -NMR and 13C -NMR studies of reduced disaccharides and tetrasaccharides...

Ngày tải lên: 16/03/2014, 14:20

... Glu, Ser and Thr residues, the absence of Lys, His and Arg except at the termini, and the fact that it is completely unstructured based on the absence of medium- and long-range NOEs and the observation ... including the ‘BG loop’ and the ‘G’-strand (Fig 2) [24] Hortner et al [25] have modelled the structure of the SOCS3 SH2 domain and suggest that the BG loop and bG strand are formed from residues ... ms and a NOESY mixing time of 120 ms were used Spectra were processed using xwinnmr (Bruker AG, Karlsruhe, Germany) or nmr- pipe [44], and were analysed using xeasy (version 1.3.13) [45] or nmrdraw...

Ngày tải lên: 16/03/2014, 14:20

... common and studied CDs are a-, b- and c-CDs, consisting of 6-, 7- and 8-glucosyl units, respectively [14] CDs can be described as toroidal, hollow, truncated cones with a hydrophilic surface and ... drugs, control of volatility and sublimation, physical isolation of incompatible compounds, long-term protection of color, odor and flavor, and suppression of hemolysis and the bitter tastes of drugs ... conditions of pH, buffer and temperature In addition, NMR and DLS analyses demonstrated the characteristic properties of GA Our results of the molecular interaction between GA and c-CD can be correlated...

Ngày tải lên: 16/03/2014, 14:20

... template and the oligonucleotides FAD(CA)-1 and FAD(CA)-2 as primers (Table 1) The 1006-bp amplication product was digested with EcoRI and BamHI and ligated into the vector pMal-c2 (New England Biolabs) ... 31P -NMR spectrum of wild-type TrxR in the oxidized (A) and the reduced state (B) Table 31P -NMR chemical shifts (in p.p.m.) of native and native reconstituted thioredoxin reductase (TrxR), and ... interpretation and the assignment of the chemical shifts are based on published 13C- and 15N -NMR studies on proteinbound and free avins [17], the data referring to free avins are also given in Tables and...

Ngày tải lên: 16/03/2014, 16:20

... sequencing In both its E- and Z-configurations, peptide was characterized further by NMR spectroscopy peptide charges because bands with nearly the same charge (bands 1B, 1C and 2) migrated quite ... within the intact a-PEC and the derived peptides The excellent solubility of peptides and at pH 7.0 recommended their employment for further studies such as crystallization and NMR spectroscopy Unfortunately, ... the peptide and the chromophore The interpretation of the NMR spectra is rather difficult because protein and chromophore signals overlap Obviously, the presence of two peaks between 10 and 11 p.p.m...

Ngày tải lên: 17/03/2014, 10:20

... The combined NMR, FTIR, fluorescence, and colorimetric studies point to the significance of segregation between the charged and hydrophobic regions of lytic peptides to membrane binding and its disruption, ... the peptide and secondary structure determination Figure summarizes sequential and medium range NOEs as well as slow-exchanging amide protons for K4L7W and its diastereomer in DPCho and SDS micelles ... peptide and membranes was obtained upon examination of the NMR- calculated average peptide conformations, displaying the relative positions of the two central lysine residues (Lys-5 and Lys-9) and...

Ngày tải lên: 17/03/2014, 11:20