monitoring murine skeletal muscle function for muscle gene therapy

... physiology assays of skeletal muscle and heart function Technology breakthroughs are the driving force in muscle gene therapy Early muscle gene transfer studies were largely performed using vectors ... Encoding the mSEAP Reporter Gene 63 Jérôme Poupiot, Jérôme Ausseil, and Isabelle Richard   Monitoring Murine Skeletal Muscle Function for Muscle Gene Therapy 75 Chady H ... Association for the funding of muscle gene therapy studies in my laboratory I also thank the Parent Project Muscular Dystrophy and Jesse’s Journey, The Foundation for Gene and Cell Therapy for their...

Ngày tải lên: 29/06/2014, 09:20

... important for the resistance to possible introduction of genes using viral vectors or for the resistance to oncolytic gene therapy We believe that this finding can be of crucial interest for the ... viral gene therapy approaches Adenoviruses and Adeno-Associated viruses are used to deliver genes to tumor cells with the goal of modifying the phenotype, as for example, by introducing suicide genes ... primers: Ad type forward primer 5’-AACCGAAGGCTGCATTCACT, reverse primer 5’-ACCGCACAGGGTCTTAATAGAG Following denaturation at 96°C for 10 min, cycling conditions were 96°C for 15s, 60°C for for 40 cycles...

Ngày tải lên: 18/06/2014, 16:20

... enables them to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug ... consist of several aspects: one is from its transgene that encodes prodrug converting enzymes for suicide -gene therapy or cytokines for immuno -gene therapy These are essentially the same as the ... vectors for cancer gene therapy At present, there are various gene therapy vector systems under development against cancer However, due to the complexity of the solid tumours involving angiogenesis,...

Ngày tải lên: 14/08/2014, 19:22

... source for MSCs and NSCs in suicide gene therapy 113 5.1.1 Baculoviruses as gene therapy vectors for human cancer 113 5.1.2 HESC- derived MSCs and NSCs as gene delivery vehicles for glioma therapy ... 1.1 Percentage of each gene therapy strategy for clinical trials (Michael Edelstein, 2012) 1.1.2 Gene carriers for cancer therapy Successful gene therapy relies largely on gene delivery efficiency ... for cancer gene therapy 24 1.2.3 New reliable sources for therapeutic stem cells 26 1.3 Current mouse models in cancer research 28 1.3.1 Syngeneic models 28 1.3.2 Xenogeneic models 29 1.3.3 Genetically...

Ngày tải lên: 09/09/2015, 10:08

... vectors in gene therapy for glioma Finally, this study demonstrated, for the first time, that human embryonic stem cells can provide a potentially unlimited source for glioma gene therapy Using ... cell vectors in glioma gene 20 therapy 1.3.6 Stem cell based glioma gene therapy 1.3.6.1 Therapeutic genes 22 22 1.3.6.1.1 Prodrug-converting enzymes 22 1.3.6.1.2 Other gene payloads 25 1.3.6.2 ... therapies for degenerative diseases such as diabetes and Parkinson’s disease Finally, human ESCs provide an unlimited supply of cellular vectors for novel ex vivo gene therapy After genetic modification...

Ngày tải lên: 11/09/2015, 09:00

... target for selective gene therapy As gene therapy for GBM is localised there is minimum risk of systemic toxicity (Pulkkanen and Yla-Herttuala, 2005) Gene therapy is the transfer of exogenous genes, ... of survival Generally there is no curative therapy for GBM and long-term control is rarely achieved with current therapies (Pulkkanen and Yla-Herttuala, 2005) 3    1.2 Gene Therapy for GBM Although ... for Glioblastoma .3 1.2 Gene therapy for GBM 1.3 Transcriptional Targeting 1.3.1 Tissue-specific promoters .7 1.3.2 Tumour-specific promoters 1.4 Vectors for Gene...

Ngày tải lên: 22/10/2015, 21:20

... reliable device for measuring skeletal muscle viscoelastic parameters; therefore, such electro-mechanical characterization of the skeletal muscle may provide new insights into muscle function and ... rate monitoring and to assess muscle fatigue of workers after one week work cycle applying myotonometric method and special equipment to perform biomechanical diagnostics of functional state of skeletal ... milliseconds, tk in Fig 3), which was forwarded to the contact area For our study, the duration of the impact on the muscle belly of studied muscles was 15 milliseconds The force impulse terminated with...

Ngày tải lên: 20/06/2014, 00:20

... were performed for both the uninjured, and the injured limb Determined functional parameters for the uninjured limb were considered to be the patients individual muscle strength Muscle function ... restitution of skeletal muscle contraction force, and the restoration of intramuscular energy resources are major determinants for the outcome These influence the return of muscle function and ... 24 hours and the functional outcome in muscle performance after weeks There was no correlation between muscle function and the intracompartimental pressure itself Alterations in muscle perfusion,...

Ngày tải lên: 20/06/2014, 01:20

... tissue samples were taken for isolation of mitochondria from the liver, muscle and kidney For technical reasons, one sample of the liver and two samples from the skeletal muscle from endotoxemic ... mitochondrial membrane [9,11] In humans, skeletal muscle mitochondrial dysfunction has been related to severity of sepsis and poor outcome [15] Although mitochondrial dysfunction has been found in the ... available for analysis In addition, samples for the study of kidney mitochondria were taken in the last 15 experiments only (control, n = 8; endotoxemia, n = 7) Tissue samples for mitochondrial function...

Ngày tải lên: 13/08/2014, 02:24

... were checked for normality Non-normally distributed variables were log-transformed, checked for normality after log transformation, and used as continuous log-transformed variables for analyses ... exercise subjects (n=5) for whom skeletal muscle tissue was available Figure Int J Med Sci 2006, shows the immunohistochemical determination of hSGLT3 in skeletal muscle before (Figure 2A) and after ... fashion with the exception of muscle strength hSGLT3 and GLUT4 gene expression RNA Extraction Skeletal muscle samples were obtained in the non-dominant vastus lateralis muscle by percutaneous needle...

Ngày tải lên: 31/10/2012, 17:08

... separation for muscle proteomics As only limited technical information exists on the specific identification of skeletal muscle proteins by proteomics analysis [34,35], we followed the general practical ... J.S (2002) Gene therapy of muscular dystrophy Hum Mol Genet 11, 2355–2362 15 Van Deutekom, J.C & van Ommen, G.J (2003) Advances in Duchenne muscular dystrophy gene therapy Nat Rev Genet 4, 774–783 ... potential of gene therapy for Duchenne muscular dystrophy Hum Mol Genet 9, 2507–2515 13 Wang, B., Li, J & Xiao, X (2000) Adeno-associated virus vector carrying human minidystrophin genes effectively...

Ngày tải lên: 19/02/2014, 16:20

... 3.16 for the inhibition in the presence of 18 nm [3H]ryanodine Chevallier et al found a Kd value of 6.5 lm for the La3+ (ion which has similar properties to Gd3+) in competition with calcium for ... would significantly worsen the signal to noise ratio because Ca2+ (for which the RyR1 has much lower conductance than for K+) is performing a larger role as a current carrier Our results suggest ... for the visualization of the protein composition of the fractions Isolation of heavy SR vesicles Ryanodine-binding Procedure to obtain muscle was in accordance with the ethical standards as formulated...

Ngày tải lên: 19/02/2014, 16:20

... Rafael-Fortney JA (2006) Analysis of gene expression differences between utrophin/dystrophin-deficient vs mdx skeletal muscles reveals a specific upregulation of slow muscle genes in limb muscles ... forming the muscle differs between muscles: the quadriceps muscle is composed roughly of 50% type fibres and 50% type fibres, the TA and EDL muscles contain about 5–10% type fibres, the soleus muscle ... Matsuda J, Aburatani H et al (2004) Skeletal muscle FOXO1 (FKHR) transgenic mice have less skeletal muscle mass, downregulated type I (slow twitch/red muscle) fiber genes, and impaired glycemic control...

Ngày tải lên: 07/03/2014, 03:20

... some of its isoforms in several different forms of cancer [15–20] Skeletal muscle tissue contains high levels of phosphocreatine, creatine and CK, and this tissue can be transformed into malignant ... isoforms are expressed in a tissue-specific manner [1–4] There are two cytosolic CK isoforms Whereby muscle- specific cytosolic (MCK) is expressed specifically in sarcomeric skeletal and cardiac muscles, ... in striated skeletal and heart muscles and ubiquitous uMitCK is present in smooth muscle, brain, neuronal and other non -muscle Table Creatine and phosphocreatine content in normal muscle (NM)...

Ngày tải lên: 07/03/2014, 06:20

... p(r) function indicated an elongated (prolate) shape for the protein conformation The general behaviour for the p(r) function was similar for the two proteins, and the major differences occur for ... lacking in this model and more structural information is necessary for full comprehension of Tn function To investigate the interactions between skeletal muscle TnC(1–91) and TnI(98–182), we have ... a-helical conformations TnC and the residues corresponding to the skeletal TnI 117–160 in cTnI have high a-helical content [6,20] The chimeras had functional similarity with the experiments performed...

Ngày tải lên: 16/03/2014, 18:20

... another cause of the decreased regenerative potential of aged skeletal muscle Indeed, enhancement of Notch-1 signaling promotes muscle regeneration in old skeletal muscle [8, 9] Although these experiments ... 1/3 genes The majority of SRF’s targets are genes involved in cell growth, migration, cytoskeletal organization, and myogenesis [100, 101] SRF was first shown to be essential for both skeletal muscle ... expressed in skeletal and cardiac muscle Some of them are found specifically, or at least are highly concentrated, in skeletal and/or cardiac muscle, suggesting 18 Skeletal Muscle – From Myogenesis...

Ngày tải lên: 16/03/2014, 21:20

... coef®cient at 280 nm for a 1% solution of 6.47 for HMM [19] and at 290 nm for a 1% solution of 6.6 for ATP-G-actin [20] High pressure apparatus High pressure devices used for this study consisted ... ®bers Proc Natl Acad Sci USA 88, 7323±7327 Fortune, N.S., Geeves, M.A & Ranatunga, K.W (1994) Contractile activation and force generation in skeletal rabbit muscle ®bres e€ects of hydrostatic pressure ... protein structure and function Proteins 24, 81±91 Fortune, N.S., Geeves, M.A & Ranatunga, K.W (1991) Tension responses to rapid pressure release in glycerinated rabbit muscle ®bers Proc Natl...

Ngày tải lên: 24/03/2014, 00:21

... FFMBIS bias 4.4 Muscle mass prediction Previously published BIA-equations4,8 overestimated skeletal muscle mass in our subjects The overestimations were larger for men than for women for both equations, ... ALSTDxA ¼ appendicular lean soft tissue TBSMMDxA ¼ total body skeletal muscle mass, calculated as 1.19 Â ALSTDxA À 1.65.11 SMMIDxA ¼ skeletal muscle mass index, calculated as TBSMMDxA/(height in m2) ... was À0.97 kg for women and À0.40 kg for men However, the Bland–Altman plot revealed a significant small systematic negative bias (Fig 1b), reciprocal to the FFMBIS bias 3.4 Skeletal muscle mass...

Ngày tải lên: 28/03/2014, 20:20

... its function [55] Regulation of function through the control of subcellular localization represents novel information for a member of the calpain family, although it was previously described for ... in skeletal muscle during disuse atrophy FASEB J 16, 529–538 Judge AR, Koncarevic A, Hunter RB, Liou HC, Jackman RW & Kandarian SC (2007) Role for IkappaBalpha, but not c-Rel, in skeletal muscle ... [17,19–21] Although the three MARPs are expressed in both heart and skeletal muscle [18,22,23], Ankrd2 is mainly expressed in skeletal muscle [18,24,25], CARP in the heart [18,21,26–28] and DARP in...

Ngày tải lên: 29/03/2014, 21:20

... expressed only in skeletal muscles, and promotes muscle differentiation if induced by MyoD and myogenin during myogenesis [16,17] These muscle- specific miRNAs seem to participate in muscle diseases, ... processes, including myogenesis and muscle regeneration The three muscle- specific miRNAs, miR-1, miR133, and miR-206 have been shown to play important roles in the regulation of muscle development ... [31] It is the unique myomiR exclusively expressed in skeletal muscle [32-36] and has been rarely detectable in the heart [37-41] The skeletal muscle- specific expression of miR-206 was first clearly...

Ngày tải lên: 18/06/2014, 16:20