molecular mechanism of antigen retrieval technique

... concentration of the oligomeric species were also decreased Mechanism of neurotoxicity in FAP: the lesson from other amyloidoses A number of studies have examined the mechanism of neurotoxicity ... oligomeric species of TTR The presence of low -molecular- mass TTR aggregates was found to be correlated with the ability of TTR to induce calcium influx via voltage-gated calcium channels High -molecular- mass ... the location of amyloidogenic mutations and position of b-strands (A) The structure of the polypeptide backbone of the two chains (purple and blue) is shown along with the location of the N-terminus...

Ngày tải lên: 07/03/2014, 10:20

... representative of PAH, originating from incomplete combustion or pyrolysis of organic matter Under normal conditions, the diet is Benzo[a]pyrene inhibition of lipolysis the main source of B[a]P exposure ... little has been documented on the effect of B[a]P on adipocyte function, one of the main sites of storage We therefore decided to address the question of B[a]P toxicity on adipocytes, and specifically ... Upon withdrawal of this PAH, the excess weight gain was not corrected Our data indicate that inhibition of lipolysis by B[a]P proceeds via direct inhibition of the early step of b-adrenergic...

Ngày tải lên: 30/03/2014, 11:20

... differentiation In conclusion, control of the production of RANKL, of OPG and of other T-cell-derived cytokines in RA patients will provide a clue for strategies of the development of new treatment for inhibiting ... concentration of OPG was detected in the synovial fluids from RA patients The ratio of the concentration of soluble RANKL to that of OPG was significantly higher in the synovial fluids of RA patients ... cell tumor of bone The concentrations of both IL-6 and sIL-6R were significantly higher in the synovial fluids of patients with RA than in those of patients with OA The concentrations of IL-6 and...

Ngày tải lên: 09/08/2014, 06:22

... failure, underscoring the necessity of elucidating the molecular mechanism of PLB action D THE MECHANISM OF Ca2+ TRANSPORT BY SERCA2a SERCA is a large protein of nearly 1000 amino acids that actively ... Inhibition of Cross-Linking of Residues 45-52 of PLB to V89C-SERCA2a 17 Figure 14 Our Model of PLB Regulation of SERCA2a Activity .18 Figure 15 Complete Amino Acid Sequences of the Cross-linkable ... regulated by PLB is of great scientific and clinical interest The overall purpose of this dissertation research was to investigate the molecular mechanism of PLB regulation of SERCA2a Figure Excitation-Contraction...

Ngày tải lên: 24/08/2014, 11:06

... Disclaimer Title of Thesis/Dissertation: The Molecular Mechanism of Break Induced Replication For the degree of Master of Science Choose your degree I certify that in the preparation of this thesis, ...  The Molecular Mechanism of   Break  Induced  Replication  Major  Professor:  Anna  Malkova     DNA  double  strand  break  (DSB)  is  one of  the  most  threatening of  all  types of  DNA ...  understanding  the  underlying molecular  mechanisms of  DNA  repair  processes   is  critical  My  research,  in  trying  to  unravel  the molecular  mechanisms of  BIR  in  yeast,  is   important...

Ngày tải lên: 24/08/2014, 13:24

... MOLCULAR MECHANISM OF SELF-RENEWAL EXIT DURING ENDOTHELIAL DIFFERENTIATION QUE JIANWEN (B Med., China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF SURGERY ... endocytosis of acetylated LDL To understand the molecular mechanism of endothelial differentiation, we began by investigating one aspect of endothelial differentiation i.e inhibition of self-renewal ... differentiation 61 3.1 Study of the molecular mechanism underlying the decisionmaking between self-renewal and differentiation in stem cells 3.2.1 Introduction 3.2.2 Molecular mechanism underlying endothelial...

Ngày tải lên: 16/09/2015, 17:14

... volume (volume of induced cell culture of E coli) of PBS containing 0.1% Triton X-100, 5mM EDTA and protease inhibitor (Roche, Switzerland; every 50ml of lysis buffer requires one tablet of complete ... All spectra represented the average of three scans over the range of 190 to 250nm with a step size of 0.1nm and a band width of 1nm 2.3 T brucei 2.3.1 Culture of procyclic T brucei Procyclic, T ... 2.4.1 Isolation of mRNA from T brucei 37 2.4.2 Synthesis of first-strand cDNA 38 2.4.3 Amplification of ds cDNA by long distance PCR (LD-PCR) 39 2.4.4 Preparation of yeast competent...

Ngày tải lên: 12/10/2015, 17:35

... TABLE OF CONTENTS III SUMMARY V LIST OF TABLES VI LIST OF FIGURES VII LIST OF ABBREVIATIONS VIII CHAPTER1 INTRODUCTION 1.1 Breast cancer 1.1.1 Definition of breast cancer 1.1.2 Incidence of breast ... Removal of only the tumor and a small amount of surrounding tissue Mastectomy Removal of all of the breast tissue Prophylactic mastectomy Preventive removal of the breast to lower the risk of breast ... levels of cyclin D1 occur in a large segment of human cancers Amplification of the cyclin D1 locus accounts for a low proportion of the total number of cancers that overexpresses cyclin D1; the mechanisms...

Ngày tải lên: 12/10/2015, 17:35

... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... (Fig 5) Moreover, one of the carboxylate oxygens of Asp153 and the carboxamide oxygen of Asn153 are positioned in such a way as to allow metal coordination interaction with one of the manganese ions ... substitution, we used a modelled structure of the E coli enzyme, constructed by using homology-modelling techniques and the 3D structure of the binuclear form of B caldovelox arginase as a template...

Ngày tải lên: 21/02/2014, 01:21

... photocleavage [80] of the HC of axonemal dynein Further strong support for a functional role of the Walker motif of AAA1 is provided by molecular dissection of cytoplasmic dyneins in which mutation of the ... the processivity of cytoplasmic dynein [102–104] Molecular dissections of dynactin showed that the mechanism of processivity enhancement is not due to anchoring the motor domain of dynein to microtubules ... tail domain could be one of the mechanisms of regulation of dynein motility [94] In addition, dynein shows large variation in the mode of movements but the mode-switching mechanism is not well understood...

Ngày tải lên: 14/03/2014, 22:20

... type RNases H However, Arg118 of Sto-RNase HI is located in the same position as His124 of Eco-RNase HI, His539 of HIV-1 RNase H and Glu188 of Bha-RNase H Mutation of this residue to Ala considerably ... coordination of the metal ion A, as proposed for Glu188 of Bha-RNase H [31] The catalytic mechanism of RNase H is described in more detail below As described below under ‘Substrate recognition mechanism , ... catalytic mechanism with RNase H Substrate recognition mechanism of RNase H The crystal structure of human RNase H1 in complex with the substrate and Mg2+ highly resembles to that of Eco-RNase...

Ngày tải lên: 30/03/2014, 02:20

... M.sc National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DORCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE Singapore ... efficacy of ABT-869 can not be extrapolated from the experience of related compounds Hence, the clinical applications of ABT-869 will greatly benefit from better understanding of the molecular mechanism ... promising new class of anticancer drugs We demonstrated that combining ABT-869 with SAHA leaded to synergistic killing of AML cells with FLT3 mutations To study the molecular mechanism of their interaction,...

Ngày tải lên: 11/09/2015, 09:06

... comparison of transcriptome profiles of MV411 and THP-1 75 Figure 4.3 Hierarchical clustering of transcriptomic profiles of microarray data 78 Figure 4.4 Linear regression analysis of fold-changes of ... 1.4.4 Mechanism of action of rapa Rapamycin is a known inhibitor of the mammalian Target Of Rapamycin (mTOR) pathway The mechanism of this inhibition has been well established 1.4.4.1 Structure of ... approach, profiling the molecular level changes of mRNA and protein expression as a consequence of drug treatment has been proven to be effective in understanding the mechanism of action of drugs...

Ngày tải lên: 11/09/2015, 09:18

... those of the query to within specified limits Variations of this technique are now used in a high proportion of current CBIR systems Methods of improving on Swain and Ballard’s original technique ... the requirements for the degree of Doctor of Philosophy School of Computing and Engineering University of Huddersfield March 2015 Copyright Statement I The author of this thesis (including any ... robustness of the proposed signature of the sampled images, especially under varied conditions of, rotation, scaling, noise and light intensity To further improve retrieval efficiency of the devised...

Ngày tải lên: 10/11/2015, 09:42

... major role in the pathogenesis of both types of diabetes mellitus High levels of free radicals and the decline of antioxidant defense mechanisms lead to damage of cellular organelles and enzymes, ... effects on diabetes, hypertension and wound healing Presumably, one of mechanisms of action is its antioxidant activity The action of EPRT is to produce electrical pressure rather than an electrical ... development of the oxidative stress (68) If our findings are proven by further studies involving a larger number of patients, ultra-low microcurrent therapy might change the concept of management of chronic...

Ngày tải lên: 26/10/2012, 09:39

... Assistant Professor of Medicine and Staff Physician at the Department of Gastroenterology and Hepatology, University Hospital Freiburg, Germany Since 2004 he is the head of the Division of Gastroenterology ... independently of the pre-initiation factors necessary for cap-dependent translation The three-dimensional structure of the HCV IRES bound to the 40S ribosomal subunit illustrates the molecular basis of ... the genome from nucleic acid binding proteins Recent advances in the understanding of the molecular mechanisms of this enzyme could enable a specific inhibition as a novel antiviral strategy [47,...

Ngày tải lên: 02/11/2012, 10:00

... necessary should the rate of emergence of resistance and the impact of such mutations turn out to be clinically relevant for therapy management Molecular Mechanisms of Drug Resistance HBV is ... is the appearance of serum markers of HBV infection that establishes the diagnosis of the disease Of these, HBsAg is considered to be the sentinel marker for the confirmation of acute infection ... resistance in vitro and molecular mechanisms of nucleoside resistance Semin Liver Dis 2002;22:23-31 21 Doo E, Liang TJ Molecular anatomy and pathophysiologic implications of drug resistance in...

Ngày tải lên: 03/11/2012, 09:41

... tác giao dịch với doanh nghiệp, tức chuyển nhu cầu khách hàng thành mục tiêu Marketing Ma trận Ansoff công cụ hữu ích để xây dựng mục tiêu Marketing mục tiêu cụ thể "SMART”(Specific: Cụ thể, Measurable:...

Ngày tải lên: 08/04/2013, 17:02

Ngày tải lên: 07/06/2013, 01:26

... difficulties, not taking the other kind of techniques, i.e top-down ones These techniques were experimented over a period of one term and were applied in the three stages of a listening lesson Due to the ... Types of listening There are many different types of listening We can classify these according to a number of variables, including listening purposes, the role of the listener and the types of text ... emphasizes the active and personal nature of successful listening The mental model that we build as a representation of a spoken of a message is the result of our combining the new information in...

Ngày tải lên: 07/09/2013, 13:48