... motif of cardosin A is located at the surface of the protein [4], as seen in other structures of biologically active proteins [32,33] However, a careful examination of the X-ray structure of cardosin ... the tip of a loop protruding away from the core of the protein This amino-acid motif mimics RGD in terms of charge and is positioned at the tip of a loop and is therefore reminiscent of RGD sequences ... of cardosin A) Two proteins with apparent molecular masses 90 kDa and 67 kDa were isolated on elution with an RGDS peptide (Fig 1A) The 90-kDa protein has a molecular mass similar to that of...
Ngày tải lên: 30/03/2014, 11:20
... methylation analysis is also provided by this volume The analysis of gene expression represents a key area of research in the study of human cancer and a number of chapters in Molecular Analysis of Cancer ... assessment of prognosis and disease progression The aim of Molecular Analysis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular ... reaction: àL of DNA (150200 ng), 10 àL of PCR buffer (10X), àL of MgCl2 (25 mM), 10 àL of dNTP (2 mM), 65 àL of dH2O, àL of primer (100 mM), àL of Taq polymerase (5 U/àL) for a total volume of 100...
Ngày tải lên: 08/04/2014, 12:51
báo cáo hóa học:" Molecular analysis of the apoptotic effects of BPA in acute myeloid leukemia cells" potx
... an increase of the G1 peack at low dose BPA (30 μM) and an increase of the G2-M fraction of cells at the highest concentration of BPA (100 μM) Differently, in the K562 cells, a model of AML derived ... FACS analysis of apoptosis Apoptosis was measured with Annexin V/PI double staining detection (Roche and Sigma-Aldrich, respectively) as Page of (page number not for citation purposes) Journal of ... combination of ATRA μM and BPA, at the indicated concentrations for 48 hrs (B) Cell cycle analysis and apoptosis in K562 cells after 48 hrs of treatment with 60 and 100 μM BPA (C) Cell cycle analysis...
Ngày tải lên: 18/06/2014, 15:20
Báo cáo khoa học: " Effect of fixation time and freeze-thaw cycles on the molecular analysis of viral DNA" ppt
Ngày tải lên: 07/08/2014, 17:22
Báo cáo khoa học: "Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks" ppsx
... and 2) Analysis of hprt mutations in T-cells from spleens of control and test materials-exposed B6C3F1 mice Analysis of the spontaneous hprt mutant clones yielding The toxicologic actions of ozone, ... percentage versus the number of total clones Table DNA sequence analysis of hprt mutant in splenic cells of B6C3F1 female mice in 52-wk study Type of mutation Number of mutants Control Ozone NNK ... expected by addition of their individual responses The study of these interactions can lead to a better understanding of the toxic mechanism of the chemicals involved A number of terms have been...
Ngày tải lên: 07/08/2014, 18:20
báo cáo khoa học: " Next generation sequence analysis for mitochondrial disorders" ppt
... all cases of mitochondrial disorders [4] In current clinical practice, the diagnosis of mitochondrial disease relies heavily on identifying deficient activity of one or more of the mitochondrial ... http://genomemedicine.com/content/1/10/100 Background Mitochondrial disorders are the most common group of metabolic disorders, with an estimated prevalence of in 5,000 [1] Mitochondrial disorders may present with any ... molecular diagnosis of mitochondrial disorders by simultaneous sequencing of the entire mtDNA genome and the exons of 362 nuclear genes for targeted mitochondrial proteins The current list of targeted...
Ngày tải lên: 11/08/2014, 12:20
Báo cáo y học: " Molecular analysis of HBV genotypes and subgenotypes in the Central-East region of Tunisia" potx
... the risk of evolution to cirrhosis and HCC [11] This type of mutants seems to be frequent in our population in view of the fact that 59% of the patients are characterized by the absence of HBeAg ... genotyping In addition to the risk of erroneous results, the limit of the rapid methods using classic PCR is the low sensitivity of DNA detection (the limit of detection of our method being 10 copies/ml) ... constructed using the neighbour-joining algorithm of MEGA4.1 software, with 1000 Bootstrap replicates Results Sixty percent of patients (n = 130 out of 217) were positive by PCR amplification in...
Ngày tải lên: 12/08/2014, 02:20
Báo cáo y học: "Population phylogenomic analysis of mitochondrial DNA in wild boars and domestic pigs revealed multiple domestication events in East Asia" pdf
... current population structures of wild boars in these regions might be shaped by the early dispersal of the wild boars out of the Mekong region Our classification analysis of the published Ryukyu island ... by grants of the National Basic Research Program of China (973 Program, 2007CB815700, and 2006CB102100), Chinese Academy of Sciences (KSCX2-YW-N-018), Bureau of Science and Technology of Yunnan ... settlement of Asia revealed by analysis of complete mitochondrial genomes Science 2005, 308:1034-1036 Yao Y-G, Kong Q-P, Man X-Y, Bandelt H-J, Zhang Y-P: Reconstructing the evolutionary history of China:...
Ngày tải lên: 14/08/2014, 08:20
Molecular analysis of the roles of yeast microtubule associated genes in agrobacterium mediated transformation
... of knowledge of the molecular principles of T-DNA transfer process AMT is one of the most powerful genetic techniques for generation of transgenic plants Transgenic plants have a great many of ... the fewer amount of tubulin isoforms and the various functions of MTs in multiple molecular and cellular processes, it was proposed that the function diversity of different types of MTs could be ... VIII LIST OF MANUSCRIPTS X LIST OF TABLES XI LIST OF FIGURES XII LIST OF ABBREVIATIONS XV CHAPTER LITERATURE REVIEW 1.1 Overview of the structure...
Ngày tải lên: 09/09/2015, 10:08
LIVE TRACKING VIRE2 PROTEIN AND MOLECULAR ANALYSIS OF YEAST FACTOR PMP3P DURING AGROBACTERIUM MEDIATED TRANSFORMATION
... AND MOLECULAR ANALYSIS OF YEAST FACTOR PMP3P DURING AGROBACTERIUM-MEDIATED TRANSFORMATION LI XIAOYANG (B Sc Science.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... assay of VirE2 in yeast cells 68 Figure 3.22 GFP11 does not perturb the function of VirE2 in AMT of plants 71 Figure 3.23 Study of putative nuclear localization signals of VirE2 in AMT of ... Schematic diagram of Agrobacterium VirE2 labeling strategy 46 Figure 3.9 Schematic diagram of transgenic expression of VirE2 in yeast 48 Figure 3.10 Schematic diagram of internal labeling of VirE2...
Ngày tải lên: 09/09/2015, 11:19
Molecular analysis of genes mediating t DNA trafficking inside yeast cells
... Construction of pUPT301 Fig 3.2 Construction of pAPT301 Fig 3.3 Construction of pLPT19 Fig 3.4 Development of a method for Agrobacterium-mediated transformation of S pombe Fig 3.5 Transformation of S ... extraction and analysis Fig 3.7 Border analysis of S pombe transformants Fig 3.8 Comparison of S pombe binary vectors Fig 3.9 Comparison of S pombe and binary vectors Fig 3.10 Affect of cocultivation ... from S pombe 2.2.4 Preparation of A tumefaciens genomic DNA 2.2.5 Preparation of S pombe genomic DNA 2.2.6 Preperation of S cerevisiae genomic DNA 2.3 Analysis of potential integrant into the...
Ngày tải lên: 12/09/2015, 08:20
Molecular analysis of mutations in agrobacterium tumefaciens under selection pressure 1
... LIST OF FIGURES VII LIST OF TABLES X LIST OF ABBREVIATIONS .XI SUMMARY XIII CHAPTER LITERATURE REVIEW 1.1 Overview of the regulation of ... base substitutions; deletions, or loss of any number of base pairs; insertions, or the addition of any number of base pairs; inversions, in which a piece of linear DNA molecule is excised and ... genome of A tumefaciens 32 1.3 Some important features of two-component systems 33 1.3.1 The characteristics of sensor and regulator proteins 34 1.3.2 Activities of the HPK...
Ngày tải lên: 13/09/2015, 20:52
Molecular analysis of mutations in agrobacterium tumefaciens under selection pressure 2
... amino acids The molecular weight is 18.99 kD The molecular weight of the proteins on the two dimensional gel was almost accurate considering the position of AopB which exact molecular weight ... construction of a gene bank of Rhizobium meliloti Proc Natl Acad Sci USA 77: 7347-7351 Doty, S L., Yu, M C., Lundin, J I., Heath, J D., and Nester, E W 1996 Mutational analysis of the input domain of the ... ml of mM EDTA, pH 8.0, 0.2 mM DTT and 20% sucrose by sonication The suspension was layered onto a gradient containing a top layer of ml of mM EDTA, pH 8.0 and 53% sucrose over a bottom layer of...
Ngày tải lên: 13/09/2015, 20:53
Molecular analysis of the breeding biology of the asian arowana (scleropages formosus)
... teeth profile of a male before, during and after mouthbrooding by confocal microscopy-based analysis of dental moulds Panels: A) A planar representation of the entopterygoid teeth profile of a male ... number of offspring that was collected from the mouthbrooding 16 | P a g e individual The column on the left indicates the date of the collection of the offspring from the buccal cavity of the ... number of offspring collected (For rest of the labels, see Fig 18) 110 Figure 25: Kinship coefficient data of the thief and the genetic parents of stolen eggs did not show any indication of...
Ngày tải lên: 14/09/2015, 08:49
Molecular analysis of hibiscus chlorotic ringspot virus coat protein mediated suppression of gene silencing
... 53 2.4.2 Preparation of radio-labeled RNA probes 54 2.4.3 Preparation of infectious transcripts 54 2.5 Analysis of RNA from plants 2.5.1 Isolation of total RNA from plants ... cotransfect cells, then similar molecular analysis as in the plant protoplast system was carried out to detect the changes of high molecular weright (HMW) and low molecular weight (LMW) RNA, respectively ... to Prof Ding Shouwei and Dr Li Hongwei from University of California, Riverside, for their patience and good suggestions on my experiments Special thanks to all the members of the Plant Molecular...
Ngày tải lên: 16/09/2015, 08:31
Molecular analysis of oxysterol binding proteins in yeast
... 2.2 The molecular function of OSBP…………………………………………… 22 21 2.3 The molecular function of ORPs……………………………………………….….24 23 2.4 The molecular function of OSHs 2.4.1 Secondary structures of Osh proteins…………………………………….…… ... cholesterol transport out of ER: The ER is the site of cholesterol synthesis but it maintains a low steady-state level of cholesterol, so efficient transport of cholesterol out of the ER must exist ... than one decade since the cloning of OSBP, the molecular function of OSBP proteins remains to be defined Because of its high affinity for oxysterols and the potency of oxysterols as feedback regulators,...
Ngày tải lên: 16/09/2015, 08:31
Molecular analysis of the p14 ARF hdm2 p53 regulatory pathway in breast carcinoma
... Mutational Analysis of p14ARF 60 2.4.4 Analysis of mRNA expression of p14ARF 61 RESULTS IN HUMAN BREAST CELL LINES 62 2.5.1 Mutational analysis of p53 62 2.5.2 Gene amplification of hdm2 63 2.5.3 ... extraction 48 2.3.4 Mutational analysis of p53 49 2.3.4.1 PCR-SSCP Analysis 49 2.3.4.2 Direct sequencing 51 iv 2.3.5 Mutational Analysis of p14ARF 51 2.3.5.1 PCR-SSCP analysis 51 2.3.5.2 Southern ... Mutational Analysis of p14ARF 64 2.5.4 2.6 RESULTS IN PRIMARY INVASIVE BREAST CARCINOMA 2.4.2 2.5 55 2.4.1 2.4 Safety precautions in use of radioactive materials Analysis of mRNA expression of p14ARF...
Ngày tải lên: 17/09/2015, 17:20
Molecular analysis of the gene LAS17 mediating t DNA trafficking inside yeast cells
... course of my research programme at the National University of Singapore i TABLE OF CONTENT Pages Acknowledgements i Table of contents ii Summary v List of Tables vi List of Figures vii List of Abbreviations ... MOLECULAR ANALYSIS OF THE GENE LAS17 MEDIATING T-DNA TRAFFICKING IN YEAST CELLS HARIPRIYA BATHULA (B.pharm, M.Tech) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL ... range of the Agrobacterium S cerevisiae is also the often used eukaryotic model organism by many scientists because of its ease of handling, the availability of vectors, and also the ease of transformation...
Ngày tải lên: 13/10/2015, 16:50
Molecular analysis of the role of a yeast potassium transport component TRK1 in agrobacterium mediated transformation
... MOLECULAR ANALYSIS OF THE ROLE OF A YEAST POTASSIUM TRANSPORT COMPONENT TRK1 IN AGROBACTERIUM-MEDIATED TRANSFORMATION NGUYEN CONG HUONG (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ... is a pathogen of plant Crown Gall disease In nature, its host varies in many species of the plant kingdom including more than 600 types of plant (56% of the gymnosperms and 58% of angiosperms ... host cells Better understanding of molecular basis of Agrobacterium-eukaryote gene transfer can help us extend the potential of diverse vector for DNA and protein of A tumefaciens This following...
Ngày tải lên: 16/10/2015, 11:57
Molecular analysis of maternal diabetes induced changes in the developing neural tube
... B dc dc e vtw vtw E C vt dt D Fig Thickness of Ventral Telencephalon Wall (µm) 300 250 * 200 150 100 50 Fig Control Diabetic vt dt vt vt A B Fig % of BrdU-cells in Ventral Telencephalon 180 * ... Fig vt B e A Fold of induction 3.5 ** 2.5 1.5 0.5 Control Diabetic B 333bp C Fig dc dc III vt A vt B LV A Fig dc e dc III vt vt LV A Fig dt B III e e e dc vt LV A Fig 10 B A Fold of induction 1.5 ... A Fold of induction 1.4 1.2 0.8 0.6 0.4 0.2 Control Diabetic B 238bp C Fig 13 A Fig 14 B C B III III A B ctx LV LV LV LV cn C D III III III E F III III sn hi dn G Fig 15 H chp bt number of microglia...
Ngày tải lên: 26/11/2015, 12:46