Molecular analysis of maternal diabetes induced changes in the developing neural tube

mb hb fb A A Fig B in e A III LV E B dc dc e vtw vtw E C vt dt D Fig Thickness of Ventral Telencephalon Wall (µm) 300 250 * 200 150 100 50 Fig Control Diabetic vt dt vt vt A B Fig % of BrdU-cells in Ventral Telencephalon 180 * 160 140 120 100 80 60 40 20 Fig Control Diabetic dc e dc vt A Fig vt B e A Fold of induction 3.5 ** 2.5 1.5 0.5 Control Diabetic B 333bp C Fig dc dc III vt A vt B LV A Fig dc e dc III vt vt LV A Fig dt B III e e e dc vt LV A Fig 10 B A Fold of induction 1.5 ** 0.5 Control Diabetic B 325bp C Fig 11 e dc e dc vt vt dmt dt A Fig 12 B * A Fold of induction 1.4 1.2 0.8 0.6 0.4 0.2 Control Diabetic B 238bp C Fig 13 A Fig 14 B C B III III A B ctx LV LV LV LV cn C D III III III E F III III sn hi dn G Fig 15 H chp bt number of microglia (/mm ) 45 40 35 30 25 20 15 10 Fig 16 ** Normal Diabetic CP-treated CP-treated diabetic TGF-ß l TGF-ß1 5A tm htms III III A B htm III TGF-ß l sn III TGF-ß l hi dn chp bt C D TGF-ß l TGF-ß l III III E F TNF-α III G Fig 17 TNF-α G III H Lectin TGF-ß1 A B C Lectin TNF-α III D Fig 18 TGF-ß1+Lectin TNF-α+Lectin III III E F tm III htms III htm A Fig 19 B 50 Mean Cell numbers/mm * 40 30 * CP-treated diabetic * 20 10 Fig 20 TNF- α TGF-β1 CP-treated TGF-β1 mRNA A 215bp TNFα B 241bp IL-1ß C TGFß 173bp D 370bp iNOS Fig 21 folds of induction 7.5 6.5 5.5 4.5 3.5 2.5 1.5 0.5 Diabetic CP-treated CP-treated diabetic TNF- α Fig 22 Normal ** ** IL-1β TGF-β1 iNOS [...]... III sn hi dn G Fig 15 H chp bt 2 number of microglia (/mm ) 45 40 35 30 25 20 15 10 5 0 Fig 16 ** Normal Diabetic CP-treated CP-treated diabetic TGF-ß l TGF-ß1 5A tm htms III III A B htm III TGF-ß l sn III TGF-ß l hi dn chp bt C D TGF-ß l TGF-ß l III III E F TNF-α III G Fig 17 TNF-α G III H Lectin TGF-ß1 A B C Lectin TNF-α III D Fig 18 TGF-ß1+Lectin TNF-α+Lectin III III E F tm III htms III htm A Fig... CP-treated diabetic * 20 10 0 Fig 20 TNF- α TGF-β1 CP-treated TGF-β1 mRNA A 215bp TNFα B 241bp IL-1ß C TGFß 173bp D 370bp iNOS Fig 21 folds of induction 8 7.5 7 6.5 6 5.5 5 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 Diabetic CP-treated CP-treated diabetic TNF- α Fig 22 Normal ** ** IL-1β TGF-β1 iNOS .. .in e A III LV E B dc dc e vtw vtw E C vt dt D Fig Thickness of Ventral Telencephalon Wall (µm) 300 250 * 200 150 100 50 Fig Control Diabetic vt dt vt vt A B Fig % of BrdU-cells in Ventral... vt B e A Fold of induction 3.5 ** 2.5 1.5 0.5 Control Diabetic B 333bp C Fig dc dc III vt A vt B LV A Fig dc e dc III vt vt LV A Fig dt B III e e e dc vt LV A Fig 10 B A Fold of induction 1.5... TGF-ß l TGF-ß l III III E F TNF-α III G Fig 17 TNF-α G III H Lectin TGF-ß1 A B C Lectin TNF-α III D Fig 18 TGF-ß1+Lectin TNF-α+Lectin III III E F tm III htms III htm A Fig 19 B 50 Mean Cell numbers/mm