mice were more resistant to neurotoxicity induced by mptp

... (Figure 5) DAI is required for virally -induced production of neurotoxic mediators by microglia and astrocytes To begin to establish a role for DAI in the inflammatory CNS damage associated with neurotropic ... [16,19] Importantly, this cytosolic sensor has been reported to mediate type I interferon and inflammatory cytokine production by HSV-1-infected murine fibroblasts [17] To date, DAI expression has ... administered to 4-6 weekold female C57BL/6 mice (Jackson Laboratories) via intranasal (i.n.) infection essentially as described by our laboratory with other viral pathogens [23,25] Anesthetized animals were...

Ngày tải lên: 19/06/2014, 22:20

... (1a–k) were more effective inhibitors than their corresponding 3H-pyrido[2,3-b]pyrazolo[3,4-h]1,6-naphthyridines (2a–c) Compounds with nearly the same antiviral effects were evaluated for cytotoxicity ... 3H-benzo[b]pyrazolo[3,4-h]-1,6-naphthyridines were more effective inhibitors than their corresponding 3H-pyrido[2,3-b]pyrazolo[3,4-h]-1,6-naphthyridines The compound 1h reduced the virus yield in 91% at 50 µM and exhibited a low cytotoxicity ... index (SI) were determined in parallel Several of the new compounds prevented the cytopathic effect of HSV-1 in Vero cells, at micromolar concentrations, and were minimally toxic to Vero cells...

Ngày tải lên: 20/06/2014, 20:20

... B VI.a M (1;1) C Dễ thấy đường thẳng (d ) : x − y − = không tạo với trục to độ góc 450 nên BC không song song với trục to độ (d ) có hệ số góc , gọi k hệ số góc k = k− = ⇔ 2k − = ⇔  BC ta ... x + y + = nhận uuu r u BH (1; −1) làm vtpt có phương trình x − y = AC cắt (d ) : x − y − = A có to độ thoả mãn hệ x − y =  x = −2 ⇒ ⇒ A(−2; −2)   x − y − =  y = −2 C đối xứng với A(−2; ... + ∞ ) hình vẽ: Số nghiệm (2) số giao điểm đường thẳng y = m với đồng thời ( P ) ( P2 ) hệ trục to độ Qua đồ thị ta thấy (2) có nghiệm [ 0; + ∞ ) m = − −1 < m < (P ) O −1 − 5/ (P ) 0,50 A H AC...

Ngày tải lên: 19/04/2015, 05:00

... was induced after exposure to (1fi3)-b-D-glucan The sponge polypeptide shares a high similarity to the related insect/ crustacean molecules, but shows no significant relationship to protostomian/nematode, ... proteins from sponges, as the basal metazoans, are more similar to the related sequences from deuterostomians (humans), than to those from protostomians (D melanogaster and C elegans) [73] In line ... from tissue were incubated for day with 10 lgÆmL)1 curdlan, then treated with 0.2% SDS, but not with 2-mercaptoethanol (the samples were not boiled prior to separation) The samples were then size...

Ngày tải lên: 16/03/2014, 16:20

... (GFP is fused to the C-terminus of nicolin 1) The protein coding regions of these two constructs were verified by DNA sequencing COS-1 cells were transiently transfected with DNA by using DEAE-dextran ... cloned into the BamHI/XhoI sites of the pEGFP-C1 or pEGFP-N1 vector (BD Biosciences Clontech) The resulting expression constructs were termed pEGFPNICN1 [green fluorescent protein (GFP) is fused to ... b-actin probes provided by Clontech were used The probes were labeled with 32P using the RadPrime labeling kit (Invitrogen, Karlsruhe, Germany) After hybridization, the membranes were exposed with...

Ngày tải lên: 23/03/2014, 21:20

... patient was submitted to an exploratory laparotomy through a bilateral subcostal incision She underwent distal pancreatectomy, splenectomy, bilateral Table Preoperative and postoperative serum hormonal ... CA-125 40 Page of adrenalectomy and nephrectomy of the ectopic left kidney Parathyroidectomy was postponed until the development of clinical or laboratory findings compatible to hyperparathyroidism ... neuroendocrine markers (chromogranin and synaptophysin), while the proliferation index assessed by Ki-67 immunohistochemistry was about 5% Taking into account the above histological observations (especially...

Ngày tải lên: 09/08/2014, 01:24

... an amino-terminal domain, the tryptophan-tryptophan (WW) domain, which is characterized by two tryptophan residues separated by 22 amino acids that can bind to phosphorylated serine- or threonine-proline ... sensitivity of iNOS to calpain [6] PIN1 may also regulate expression of inflammatory proteins by an effect on calpain Cyclooxygenase (COX)-2 is induced by LPS, IFN, and other factors in endothelial ... endothelial COX-2 may contribute to vascular pathogenesis [15,16] This enzyme is also significant for endotoxin action as COX-2 knockout mice are resistant to LPS -induced inflammation and death...

Ngày tải lên: 11/08/2014, 08:22

... inhibited by RNAi In order to accurately detect the variations of virus numbers in infected cells, specific primers were designed to target HSV-1 α-4, TK and gC genes These primers were employed to ... cells was inhibited by pGE-CTMP containing siRNA targeted specifically to CTMP-7 mRNA Cells transfected by pGE-CTMP or pGE-1 were harvested after 48 h, and lysates were analyzed by Western blotting ... enveloping by deducing that the viral capsids are directly transported to the Golgi secretory pathway and out of the cell by exocytosis [25] Certainly, no matter which of the two models is more plausible,...

Ngày tải lên: 12/08/2014, 04:20

... blood of humans are resistant to HSV-1 infection [20-22] To determine whether murine bone marrow-derived DCs were also resistant to HSV-1 infection, DCs were isolated from BALB/c mice and cultured ... BALB/c mice Similar to DCs, BM-derived macrophages have also been reported to be nonpermissive to HSV-1 infection [24-28] To determine if BM-derived macrophages from BALB/c mice were also resistant ... STAT1-/- mice and parental 129SVE mice and infected with 10 PFU/cell of HSV-1 strain McKrae Similar to the results described above for DCs, macrophages from STAT1-/- mice were more susceptible to HSV-1...

Ngày tải lên: 12/08/2014, 04:21

... blood of humans are resistant to HSV-1 infection [20-22] To determine whether murine bone marrow-derived DCs were also resistant to HSV-1 infection, DCs were isolated from BALB/c mice and cultured ... BALB/c mice Similar to DCs, BM-derived macrophages have also been reported to be nonpermissive to HSV-1 infection [24-28] To determine if BM-derived macrophages from BALB/c mice were also resistant ... STAT1-/- mice and parental 129SVE mice and infected with 10 PFU/cell of HSV-1 strain McKrae Similar to the results described above for DCs, macrophages from STAT1-/- mice were more susceptible to HSV-1...

Ngày tải lên: 12/08/2014, 04:21

... cầu: - Củng cố to n kiến thức tập hợp - biết vận dụng phép to n tập hợp biết dùng biểu đồ Ven để biểu diễn quan hệ tập hợp phép to n tập hợp - rèn luyện kĩ thực hành giải dạng to n liên quan ... NX nh SGK để tới KL : sai số tuệt đối không hoàn to n phản ánh đợc chấtlợng phép đo đạc hay tính to n Để so sánh độ xác phép đo đạc hay tính to n ngời ta dùng sai số tơng đối - 26 - * Nếu a ... ,ngôn ngữ tập hợp để diễn tả điều kiện lời to n - Biết tìm giao, hợp, hiệu , phần bù tập hợp cho - Biết sử dụng sơ đồ Ven để minh hoạ quan hệ tập hợp phép to n tập hợp Về t thái độ: - Rèn luyện t...

Ngày tải lên: 26/06/2013, 01:25

... ] = (−3; − 6; 6) = −3(1; 2; − 2) = −3.n, n = (1; 2; − 2) Mặt phẳng (ABC ) qua A ( 0;1; ) có vecto pháp tuyến n = (1; 2; − 2) nên có phương trình x + 2y − 2z − = S ABC = + 2t + 2(−2 − t ) − 2(3...

Ngày tải lên: 30/08/2013, 13:58

... KKSCLTHIIHCNMHC2012ư2013 HNGDNCHMMễN:TONư KHIA,A1 I.LUíCHUNG: ưHngdnchmchtrỡnhbymtcỏchgiivinhngýcbnphicú.Khichmbihcsinh lmtheocỏchkhỏcnuỳngvýthỡvnchoimtia. ưimtonbitớnhn0,25vkhụnglmtrũn. ưVibihỡnhhcnuthớsinhkhụngvhỡnhphnnothỡkhụngchoimtng ... ưimtonbitớnhn0,25vkhụnglmtrũn. ưVibihỡnhhcnuthớsinhkhụngvhỡnhphnnothỡkhụngchoimtng ngvi phnú. II.PN: Cõu í Nidungtrỡnhby im a 1,0im TX: D = Ă\ {2}. Giihn,timcn: ử ổ ổ lim y= lim ỗ + lim ữ = xlim y= xđ-Ơ ỗ + ữ = x đ+Ơ...

Ngày tải lên: 05/09/2013, 10:46

... ] = (−3; − 6; 6) = −3(1; 2; − 2) = −3.n, n = (1; 2; − 2) Mặt phẳng (ABC ) qua A ( 0;1; ) có vecto pháp tuyến n = (1; 2; − 2) nên có phương trình x + 2y − 2z − = S ABC = + 2t + 2(−2 − t ) − 2(3...

Ngày tải lên: 12/12/2013, 16:15

... I(3; 1), bỏn kớnh R = ỡd ( I , D) = ù ộ a = 2, b = -1, c = -10 ộ D : x - y - 10 = Gi s (D): ax + by + c = (c 0) T: ị a = 1, b = 2, c = -10 ị D : x + y - 10 = ở ùcos(d , D) = ợ uuu r uuu r 2)...

Ngày tải lên: 24/12/2013, 16:15

... DEFINITIONS To cram: To fill Mourner: Person who attends a funeral as a relative or friend of the dead person Ex: Past, previous To sadden: To upset To crowd: To gather To sink: To fall To step out: To ... associations when you want to remember anything VOCABULARY To enhance: To develop To rely on: To trust To risk: To take the risk of To utilize: To use Keen: Willing, enthusiastic, eager Eager: Ready/ ... VOCABULARY To undergo: To go through, to experience Rapid: Quick, fast To adapt: To make suitable for To grumble: To complain Generation gap: Failure of the younger and older generations to understand...

Ngày tải lên: 25/01/2014, 22:20

... via plasmid pOCA2 were selected by their resistance towards chloramphenicol and an acquired tolerance towards the radical scavenger hydroxyurea (HU) [4,9] Following this protocol, single colonies ... 6.6) in a total volume of 10 lL, and reaction was started by addition of 0.25 nmol CDP to the holoenzyme After 0.5 min, the reaction was stopped by boiling and the mixture was digested by alkaline ... followed by brief boiling to destroy the pronase The nucleotides in the reaction mixture were converted to the corresponding nucleosides by alkaline phosphatase [4] Deoxyribonucleosides were separated...

Ngày tải lên: 15/02/2014, 01:20

Ngày tải lên: 19/02/2014, 06:20

... Anti-mitogenic action of EPA and DHA (Eur J Biochem 271) 4463 proliferation of SMC by modulating the mitogenic signal transduction induced by platelet-derived growth factor (PDGF) [15], serotonin ... levels of the total cDNA templates in different samples were normalized by measuring GAPDH gene expression The oligonucleotide primers used to quantify Egr-1 and GAPDH mRNAs were identical to those ... extracts were prepared as described above Equal amounts of protein (20 lg) were fractionated by SDS/PAGE and transferred to poly(vinylidene difluoride) membranes Free binding sites were blocked by incubation...

Ngày tải lên: 19/02/2014, 16:20