methodological details for chang chun case study
Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems
Ngày tải lên :
10/09/2015, 15:53
... parasite from one form to other are not known either 1.4.1 (a) Vacuolar form The vacuolar form is also referred to as vacuolated or central body form It is the most commonly reported form of Blastocystis ... membrane bound PFOR/PNO are needed for the activation of nitroimidazoles into their active forms (Upcroft and Upcroft, 2001) PFOR/PNO are key mediators of anaerobic respiration and crucial for the survival ... micrographs representing four morphological forms of Blastocystis A) Vacuolar (V) and Multivacuolar (MV) forms B) Granular form (G) C) Cyst (arrow) D) Amoeboid form (arrow) * Adapted from Chen et al...
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Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
Ngày tải lên :
17/03/2014, 10:20
... GTP, 40% glycerol) for 30 min, then centrifuged at 100 000 g for 10 at 27 °C The pellet and supernatant fractions were processed to measure radioactivity bound to protein, and for immunoblot using ... alters the ability of the molecule to form microtubules For this purpose, we studied the behavior of nitrotyrosinated tubulin (and of tyrosinated tubulin for comparison) during reconstitution ... Carlos E Argarana and Mario Guido for critical ˜ reading of the manuscript, Mrs S N Deza and Mrs M G Schachner for technical assistance and Dr Stephen Anderson for English editing of the manuscript...
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Báo cáo y học: " Draft genome sequence of the Daphnia pathogen Octosporea bayeri: insights into the gene content of a large microsporidian genome and a model for host-parasite interactions" pps
Ngày tải lên :
09/08/2014, 20:20
... Genomes; NCBI: National Center for Biotechnology Information; ORF: open reading frame Authors' contributions NC conceived the study, performed molecular and bioinformatics analyses, contributed ... excellent and well-recognized model to study host-parasite interactions [41] The complementary nature of both genomic datasets will therefore form a great study system and provide a unique opportunity ... samples required for sequencing JFP performed bioinformatics analyses and drafted the manuscript DE provided the raw sequence data on which all presented analyses have been performed and drafted...
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Things I Learned The Hard Way: The business adventures of a freelancer
Ngày tải lên :
02/07/2014, 21:42
... never ever work for free unless it is for your mom Monday June 12 @matuvu Find Real Clients when you can, ditch the friends, family and fools they’re perfect for starters, but bad for pro’s Monday ... Monday June 12 @matuvu Save For Later don’t spend to ‘save taxes’ if you pay taxes you keep 50% if you spend you keep nothing Monday June 12 @matuvu Live The Now make time for your personal life ‘later’ ... time for your personal life ‘later’ = ‘now’ it is gone before you know Monday June 12 @matuvu Most Of All Be Flexible be prepared to fail, to change, to adapt sometimes stuff works, sometimes not...
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Báo cáo y học: "The NFI-Regulome Database: A tool for annotation and analysis of control regions of genes regulated by Nuclear Factor I transcription factors" pdf
Ngày tải lên :
10/08/2014, 09:22
... tables provide static information, and one table is used for holding user information Each group is responsible for acting as the data warehouse for a specific set of information The AuthorDB ... therefore it is listed here only as a gene Clicking on the gene link in the search menu will display binding site information for the gene From this page one can also perform searches of NCBI for ... TF_connect_TFBS_DB form the main grouping of tables used for holding gene and binding site information The TF_connect_TFBS_DB acts as a directory to allow a gene to know what binding sites it has and for a...
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The Marketing Strategy of a multinational join stock company.doc
Ngày tải lên :
27/10/2012, 16:51
... parties, and (4) something to exchange Here’s what Berkowitz stated in his book “Marketing” As marketing is a kind of exchange, certain conditions must exist before the exchange can occur 1.1.3 The ... and Places Customers Wants and needs Health, safety, comfort, transportation, beauty, productivity, etc Figure 1.1: the exchange process For marketing to occur, at least four factors are required: ... cost, manufacturing changes, or promotional modification But it Promotion can be costly in the long run if products fail to satisfy foreign consumers Do Do not not Product change Adapt product...
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Tài liệu Issues Involved When Updating the Primary Key of a Parent Row pptx
Ngày tải lên :
24/12/2013, 01:17
... tab for the foreign key named FK_Orders_Customers that contains the details of the foreign key between the Orders and Customers tables As you can see, these two tables are related through a foreign ... table) is also made to the foreign key column of the corresponding rows of the foreign key table (the child table) For example, assume this box is checked and you changed the CustomerID in the ... UpdateRule is set to Cascade; therefore, when you change the DataColumn in the parent DataTable on which the ForeignKeyConstraint was created, then the same change is also made in any corresponding...
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
Ngày tải lên :
19/02/2014, 16:20
... of the native enzyme These kinetic changes were assumed to be linked to conformation changes upon association of GAPDH with CP12 [28], which would be essential for the binding of PRK and assembly ... physico-chemical forces involved in the assembly process of this higher order structure Acknowledgements The authors thank Dr Owen Parkes for editing and Dr Luisana Avilan for help in preparing and for critical ... experiments were performed with the GAPDHCP12 complexes obtained with the mutant GAPDHs to see whether the lack of complex reconstitution could be linked to the absence of conformation changes when...
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Tài liệu Báo cáo khoa học: "Learning the Latent Semantics of a Concept from its Definition" pptx
Ngày tải lên :
19/02/2014, 19:20
... ldavec’s parameters are tuned as α = 0.05, β = 0.05 We run WMF on WN+Wik for 30 iterations, and LDA for 2000 iterations For Total 40.7 56.0 58.6 60.5 60.1 62.1 33.5 52.3 53.5 55.8 55.4 57.4 25.6 ... performance Same as (Sinha and Mihalcea, 2007), values of elesk larger than 240 are set to 1, and the rest are mapped to [0,1] elesk vs wmfvec: wmfvec outperforms elesk consistently in all POS cases ... wmfvec: ldavec also performs very well, again proving the superiority of latent semantics over surface words matching However, wmfvec also outperforms ldavec in every POS case except Semcor adverbs...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... phenolic hydroxyl is not necessary for maximal activity and mutants display no overall change in structure Importantly, Y34 can not be the sole source of protons for the dismutation reaction, although ... FXa, the purified SOD being present in the through-wash Buffer exchange and concentration was performed as described above Assay for superoxide dismutase activity The specific activity of SOD was ... purified SOD samples stained for SOD activity Lane contains FeSOD and MnSOD markers (from Sigma, arrowed, lg each), lanes 2–9 as for lanes A1 to A8, lanes 10–13 as for lanes C1 to C4 been derived...
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NATIONAL REPORT OF MALAYSIA ON THE FORMULATION OF A TRANSBOUNDARY DIAGNOSTIC ANALYSIS AND PRELIMINARY FRAMEWORK OF A STRATEGIC ACTION PROGRAMME FOR THE BAY OF BENGAL potx
Ngày tải lên :
06/03/2014, 15:21
... and water resources • Forestry Department Responsibility to manage state gazetted forests, peat wetlands and mangrove forests as well as catchment areas and rivers within forests It also controls ... negative overall trend (deteriorated) for the period 1988-1994 The overall water quality index, measured for its physical, chemical and biological characteristics in form of turbidity, salinity, temperature, ... SILANG MYSELLA CITTA DI SAVONNA ASIAN ESSO MERSIA FORTUNE LIMA MT OCEAN TREASURE BAYAN PLATFORM BRIGHT DUKE/MV PANTAS MV STOLT ADV ELHANI PLATFORM GOLAR LIE NAGASAKI SPIRIT EVOIKOS / ORADIN GLOBAL...
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The Making of a European Constitution Judges and Law Beyond Constitutive Power docx
Ngày tải lên :
07/03/2014, 02:20
... death-knell has been sounded for constitutional aspirations A European polity does not exist For all of the European Convention’s efforts to forge a common European desire for and interest in ‘closer ... translation of fact into norm See, for a US-based effort to transform constitutional theory in these terms, Griffin (1999) However, see, also, Chapter (II) for the methodological usefulness of historical ... ECR 1227 (Case 178/84) 81n34 Cordoniu SA – Cordoniu SA v Council [1994] ECR I-1853 (Case C-309/89) 155n48 Costa v ENEL [1964] ECR 585 (Case 6/64) 49–51, 78n17, 79n25, 102n23 Table of cases Dassonville...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
Ngày tải lên :
07/03/2014, 14:20
... monitoring product formation Ó FEBS 2003 at four time points for each reaction (product formation was linear for at least 20 min, at all substrate concentrations, at almost all pH values and for all mutants) ... explanation for the low pKa value for Asp215 Previous studies have shown that the software used for pKa calculations in this study can yield accurate and useful results [34,38,39], particularly for glycoside ... we have conducted a mutagenesis study of ChiB from S marcescens, for which a wealth of structural information is available [6,21,32] We employed a sensitive assay for enzyme activity, which permitted...
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Báo cáo khoa học: "Techniques to incorporate the benefits of a Hierarchy in a modified hidden Markov model" pptx
Ngày tải lên :
08/03/2014, 02:21
... calculate the transformed observation (O) for each internal state; II) apply the forward algorithm to estimate the state probabilities (¯ for the three b) transformed states; III) reform the transition ... describes the method for training HHMMs Section describes the flattening process for reducing the depth of hierarchical structure for HHMMs Section discusses the use of HHMMs for the text chunking task ... the user for better performance Table shows the observation dependency values of terms for part-of-speech tags for Figure The term NN1 IO has a higher dependency value than JJ NN1, therefore state...
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Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
Ngày tải lên :
08/03/2014, 09:20
... used for subsite mapping of endopolygalacturonases [7] We have been studying the action pattern of endo-amylases by product analysis, therefore the procedure of Allen & Thoma [5] was applied for ... their theory Subsite mapping is simplified for exo-enzymes because there is only one productive binding mode for each substrate However, endo-acting enzymes form more productive binding modes resulting ... pattern The relative rate of formation of each product is called bond cleavage frequency (BCF), which gives information about the subsite-binding energy By using BCFs for a series of oligomeric...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
Ngày tải lên :
08/03/2014, 22:20
... bond [23] The substitution of these hydrogen bond forming residues in hTRXL-N may account for the formation of a monomer, instead of a dimer in the case of TRX Furthermore, the loss of intermolecular ... crystal forms and dissimilar intermolecular contacts near the active site in the crystal, the conformation of the active site (-Cys-Gly-ProCys-) of the hTRXL-N determined in the present study is ... library (ClonTech) for full-length cDNA Three positive kDR2 phage clones were isolated and converted to pDR2 plasmid, as described in ClonTech’s manual DNA sequencing was performed according to...
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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
Ngày tải lên :
08/03/2014, 22:20
... 0.2 lCi for [c-32P]ATP, lCi for [c-32P]GTP, lCi for [c-32P]CTP, and 0.5 lCi for [c-32P]dATP Molecular masses (kDa) are indicated on the right of the panel Samples using puried enzyme before subunit ... with (+) Nucleotides were added at concentrations of 0.2 lCi for [c-32P]ATP, lCi for [c-32P]GTP, lCi for [c-32P]CTP, and 0.5 lCi for [c-32P]dATP Molecular masses (kDa) are indicated on the left ... reaction mixture was incubated at 30 C for h and then treated at 80 C for 15 in order to denature hexokinase The puried ACL incubated with nonlabeled ATP at 30 C for 15 was mixed together with the...
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Báo cáo " Influence of laser parameters on the stationary operation of a two-mode random micro laser " pdf
Ngày tải lên :
14/03/2014, 13:20
... in [12]) Table α1 = 1.1 β1 = 0.4 α = 0.9 β2 = 0.3 θ12 = 0.8 θ21 = 0.7 γ12 = 0.35 γ 21 = 0.35 For studying the influence of laser parameters on saturated values of mode photon densities, we vary ... reflects the energy transformation and the complex interaction process inside the laser powder that needs to be investigated thoroughly We also note that with a small transformation of loss coefficient, ... coefficient, the mode photon density varies clearly and quickly Therefore, loss coefficient takes the important role in the process transformating the mode photon density in random laser that has been...
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Đề tài " On the Julia set of a typical quadratic polynomial with a Siegel disk " ppt
Ngày tải lên :
14/03/2014, 22:20
... various authors in order to study rational maps with Siegel disks; see for example [P2] and [Mc2] for the case of quadratic polynomials, and [Z1] and [YZ] for variants in the case of cubic polynomials ... undergoes a contraction of the form (4.3) for all k and a definite contraction of the form (4.4) for at least every other k It follows that k+2 ≤ (1 − ε)( k + L) + L for all k Evidently this implies ... Bernstein, at least in the case ψ is increasing For a proof of the general case, see Khinchin’s book [Kh] Corollary 2.2 Let E be the set of all irrational numbers < θ < for which the sequence {an...
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