marxianus inulinase with those of glycoside hydrolase family 32 from other yeast species
Báo cáo khoa học: Aspergillus nidulans a-galactosidase of glycoside hydrolase family 36 catalyses the formation of a-galacto-oligosaccharides by transglycosylation doc
Ngày tải lên :
29/03/2014, 21:20
... parameters for hydrolysis of pNPaGal, melibiose and rafnose by AglC Parameters are calculated from the initial velocities of release of pNP from pNPaGal and of galactose from melibiose and rafnose ... transglycosylation with a-1,6regioselectivity at a lower concentration (40 mm) of melibiose and rafnose compared with a-galactosidases of Bidobacterium [3,4 ,32] and Lactobacillus [34] of the prokaryotic ... A B C 3546 Fig Stereo views of presumed ligand interactions with the active site of AglC (A) An equilibrium mixture of a- and b-galactose (pink) from the complex with O sativa a-galactosidase...
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Báo cáo khoa học: Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes doc
Ngày tải lên :
07/03/2014, 00:20
... the other hand All the different types of FTs, FEHs, CWIs and VIs are grouped together with microbial b-fructosidases (degrading both sucrose and fructans) in the glycoside hydrolase family 32 ... glycosyl hydrolases Biochem J 321 , 557–559 23 Verhaest M, Van den Ende W, Le Roy K, De Ranter CA, Van Laere A & Rabijns A (2005) X-Ray Diffraction Structure of a plant Glycosyl Hydrolase family 32 ... shown in blue (A) Details of the active sites of Ci1-FEHIIa and AtcwINV1 in complex with sucrose [26] The positions of Fru ()1 subsite) and Glc (+1 subsite) are indicated with arrows Please note...
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Tài liệu Báo cáo khoa học: His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases pptx
Ngày tải lên :
19/02/2014, 07:20
... subsite of the endoxylanase, Leu292 of TAXI-I perfectly superimposes with xylose In spite of the above, profound analysis of the specific contributions of the amino acid residues at the interface of ... previously [26] From a 100 mL culture of each of the yeast transformants, about 3–5 mg of purified protein was obtained, similar to the yield obtained for wild-type TAXI-I [26] Each of the purified ... curves of TAXI-I, alone or in the presence of endoxylanases from A niger, T longibrachiatum M2 or B subtilis (A) A niger endoxylanase, (B) a mixture of TAXI-I and A niger endoxylanase with excess of...
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Báo cáo khoa học: Characterization of the bga1-encoded glycoside hydrolase family 35 b-galactosidase of Hypocrea jecorina with galacto-b-D-galactanase activity pdf
Ngày tải lên :
16/03/2014, 11:20
... 57.80 ± 10 .32 27.93 ± 5.20 4.34 ± 0.58 6.87 ± 1.23 3 .32 ± 0.62 0 .32 ± 0.06 0.18 ± 0.05 0.13 ± 0.03 Species of Trichoderma are known to be able to colonize and grow in the rhizosphere of plants ... found in other studies [12,13] Activity of Bga1 on polymeric substrates The ability of Bga1 to hydrolyse b-galactosidic bonds in polymeric substrates was tested with two b-1,4-galactans (from lupin ... characterized a b-galactosidase from H jecorina belonging to GH family 35 Although b-galactosidases have been purified and characterized from a variety of sources [3,5,16], their GH family affiliation is...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
Ngày tải lên :
19/02/2014, 12:20
... (1998) Plant a-glucosidases of the glycoside hydrolase family 31 Molecular properties, substrate specificity, reaction mechanism, and comparison with family members of different origin Plant Mol ... subfamilies of the GH-57, reflecting the sequence similarities and/or differences, and (d) to draw an evolutionary picture, as complete as possible, of this diversified family of glycoside hydrolases ... (1999) The type II pullulanase of Thermococcus hydrothermalis: molecular characterization of the gene and expression of the catalytic domain J Bacteriol 181, 328 4 328 7 23 Erra-Pujada, M., Chang-Pi-Hin,...
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Báo cáo khoa học: Emergence of a subfamily of xylanase inhibitors within glycoside hydrolase family 18 pdf
Ngày tải lên :
07/03/2014, 17:20
... that rXIP-I consisted of three molecular isoforms of pI 6.8–7.2–8.2 with a main band at pI 7.2 This value differs from native XIP-I of pI 8.7–8.9 [6], due to the insertion of the myc epitope and ... of RIXI can be explained on the basis of the recently solved 3-D structure of XIP-I in complex with a GH10 xylanase from A nidulans and a GH11 xylanase from P funiculosum [4] The inhibition of ... Specific characterization of substrate and inhibitor binding sites of a glycosyl hydrolase family 11 xylanase from Aspergillus niger J Biol Chem 277, 44035–44043 32 Johnsson B, Lofas S & Lindquist G...
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Báo cáo khoa học: Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea pot
Ngày tải lên :
15/03/2014, 10:20
... mechanism of CcCel6C are expected to be different from those of known cellobiohydrolases Here, we present the crystal structure of CcCel6C To our knowledge, this is the first report of the crystal ... Stahlberg J (2001) Family cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic ˚ module of Cel7D (CBH58) at 1 .32 A resolution and homology models of the isozymes ... conspicuous feature of CcCel6C is that the enclosed tunnel is wider than those of HjeCel6A and HinCel6A (Fig 5A–C), and loop-1 and loop-2 of CcCel6C revealed a more open structure than those of HjeCel6A...
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Tài liệu Báo cáo khoa học: Unique features of recombinant heme oxygenase of Drosophila melanogaster compared with those of other heme oxygenases studied docx
Ngày tải lên :
19/02/2014, 12:20
... first stage of the DmDHO reaction, a hydroperoxy species is the active oxygen species, by analogy with mammalian HO-1, and this species is formed by binding of H2O2 to the ferric iron of the hemin– ... the a-meso edge of heme [39,40] and is conserved at the corresponding position of most HOs isolated from other species Sequence comparisons by FASTA searching show that DmHO is 32. 4% and 30.3% ... one of the ÔweakerÕ groups is fully conserved expressed a soluble 32 kDa protein mainly in the soluble fraction Molecular sizes of 34 kDa and 32 kDa are in agreement with the calculated values of...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
Ngày tải lên :
19/02/2014, 16:20
... conserved in the catalytic centre of GH family 28 polygalacturonases and rhamnogalacturonases [18–20], another family of inverting enzymes forming the clan GH-N with GH family 49 enzymes van Santen ... Fascinating facets of function and structure of amylolytic enzymes of glycoside hydrolase family 13 Biologia, Bratislava 57 (Suppl 11), 5–19 Aoki, H & Sakano, Y (1997) A classification of dextran-hydrolysing ... catalyst of b-xylosidase from Bacillus stearothermophilus T-6: a family 39 glycoside hydrolase FEBS Lett 495, 115–119 27 Aoki, H., Yopi, Padmajanti, A & Sakano, Y (1996) Two components of cell-bound...
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Báo cáo khoa học: Substrate recognition by glycoside hydrolase family 74 xyloglucanase from the basidiomycete Phanerochaete chrysosporium pptx
Ngày tải lên :
30/03/2014, 03:20
... respectively) (B) HPLC analysis of digestion products of TXG (C) HPLC analysis of digestion products of XXXGXXXG (D) MALDITOF MS analysis of digestion products of TXG reference [13] As described ... characteristics of a GH family 74 xyloglucanase The hydrolytic products formed from TXG by Xgh74B were analyzed by normal-phase HPLC and MALDI-TOF MS, as shown in Fig The results of HPLC suggested ... oligosaccharides with three different degrees of polymerization (DP) (Fig 4B), which showed the same retention times as oligosaccharides with DPs of 7–9, XXXG, XLXG, XXLG, and XLLG The molecular masses of...
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The syntactic functions of english adjectives in comparison with those of vietnamese ones
Ngày tải lên :
30/11/2015, 09:15
... ability of adjectives to combine with adjuncts of degree From Diep Quang Ban and Hoang Van Thung’s point of view in Ngữ Pháp Tiếng Việt (2006) and other grammarians, there are two types of adjectives ... the classification of gradable adjectives with adjectives of quality, adjectives of quantity, adjectives of characteristic, and adjectives of intensity xxvii Adjectives of quality: chăm (hard), ... types of Vietnamese adjectives which are adjectives of external features of things and adjectives of internal characteristics and states of things 3.2.1.2.1 Adjectives of external features of things...
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Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
Ngày tải lên :
18/02/2014, 06:20
... assembly with ERF ⁄ AP2 proteins is restricted to the A-6 subfamily and, in this case, even to a subset of eight members The finding that, within the A-6 subfamily, not all of its members interact with ... proteins interact with members of the ERF ⁄ AP2 family (A) Y2H assays demonstrate the assembly of BPM1(1–189) and BPM3 with RAP2.4 and RAP2.13, respectively (B) Schematic drawing of the BPM1 fragment ... up-regulation of BPM1 and BPM5 after treatment with sorbitol for h, together with increased BPM4 levels (Fig 3B) RAP2.4 also responded to both treatments with enhanced expression, which is in agreement with...
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Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx
Ngày tải lên :
07/03/2014, 17:20
... links between the N-terminal sequences of ST3 with scope subfamily, 77% with scope family, and 11% of the links with scope class (Fig 1, NN) The distribution of the hits between the C-terminal domains ... stretch of 77 residues only (see below) Therefore, the hydropathy profile of the [st326]2HCT family was aligned with the profile of the [st315]AITE family (Fig 3B) The N- and C-terminal domains of the ... Xa regions in the other families of ST3 The positions of the Vb and Xa regions were determined from the optimal hydropathy profile alignment of each family and the [st326]2HCT family (Fig 3B for...
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Báo cáo khoa học: A thermoacidophilic endoglucanase (CelB) fromAlicyclobacillus acidocaldariusdisplays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases ppt
Ngày tải lên :
17/03/2014, 10:20
... amino acid composition of the catalytic domain of CelB with those from two other members of GH family 51, EGF from F succinogenes and AbjA (Genpept accession number CAA76421) from the thermophile ... length of 410 amino acids) with endoglucanase F (EGF) from Fibrobacter succinogenes S85 ([31], GenBank accession number U39070) which belongs to family 51 of glycoside hydrolases (GH51) Among the 32 ... degradation of the substrates will likely require cooperative efforts of CelB with other glycoside hydrolases Indeed, zymogram analysis demonstrated the presence of additional protein bands with CMCase...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
Ngày tải lên :
23/03/2014, 03:20
... modelling The aim of the present work was to study the diversity of trypsins in the digestive gland of P argus, with focus on: (a) the position of lobster trypsins within the brachyurins family and ... of spiny lobsters, accounting for up to 60% of digestive proteolysis [1] We recently reported the existence of three major trypsin isoforms and other serine proteases in the digestive gland of ... sequences of PaTry1a, PaTry1b and PaTry3 were identical, and differed only in five nucleotide substitutions from the 3¢ UTR region of PaTry2 However, the 3¢ UTR sequence of PaTry4 differed to those of...
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Báo cáo Y học: Chimeric receptor analyses of the interactions of the ectodomains of ErbB-1 with epidermal growth factor and of those of ErbB-4 with neuregulin potx
Ngày tải lên :
24/03/2014, 03:21
... ErbB-3/4 are somewhat different from those of EGF [24] The determinants of NRG are clustered in a part that corresponds to the N-terminal part of EGF, while those of EGF are in two different parts: ... stimulation with EGF The extents of the autophosphorylation of 1111-1, 1111-4, 1114-4, 41111, and 4411-1, which all have the ErbB-1 domain III, were stronger than those of 1144-4 and 1444-4 without ... contributions of domains III and I in the EGF-induced autophosphorylation of ErbB-1 were fully consistent with those in the EGF binding Furthermore, the extent of the EGF-induced autophosphorylation of...
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báo cáo hóa học: " A psychometric evaluation of the PedsQL™ Family Impact Module in parents of children with sickle cell disease" pptx
Ngày tải lên :
18/06/2014, 18:20
... convenience sample of parents of children with and without sickle cell disease at both clinics as part of a larger study of the HRQL of children [13,25] There were 145 parents of children with sickle ... Quality of life among parents of children with congenital heart disease, parents of children with other diseases and parents of healthy children Qual Life Res 2003, 12:655-666 Page 10 of 11 (page ... assessing the impact of SCD on parents and families We therefore analyzed the following properties of the PedsQL™ Family Impact Module within our population of parents, both with and without sickle...
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báo cáo hóa học:" Validation of the Individualised Neuromuscular Quality Of Life for the USA with comparison of the impact of muscle disease on those living in USA versus UK" doc
Ngày tải lên :
20/06/2014, 15:20
... relationships with other close family members I require more from them.” – US patient Problems of access to the homes of friends and family and to social venues were also a source of difficulty ... Support from partner (positive) Limits on doing activities with partner Family Lack of support Worry about being a burden Worry about passing on gene Support from family (positive) Lack of understanding ... Loss of contact Support from friends (positive) General social interaction Avoidance of situation Lack of understanding 32 Difficulty explaining condition Other people’s perceptions Problems of...
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