... stopped by adding lL of EDTA (25 mm) and lL of H2O, incubating for at room temperature and further at 70 °C The DNase I treated RNA was mixed with 0.5 lg of oligo-dT2-18, lg of random primers, and ... described by Zinser and Daum [34] Protein was quantified by the method of Lowry et al [35] by using BSA as a standard SDS–PAGE was carried out as published by Laemmli [36] Western blot analysis of proteins ... sporulation of zygotes, and tetrad dissection using standard methods Identity of strains was confirmed by marker-dependent growth and colony PCR Fluorescence microscopy Visualization of mitochondrial...
Ngày tải lên: 30/03/2014, 04:20
... permeameter and sampling of the examined forest soil profiles with uniform metal cylinders were carried out Saturated hydraulic conductivity of forest soils affected by the operations of a universal ... of to cm in diameter and depth up to m is bored into soil and the outlet part is inserted; the authors used a hole of cm in diameter and of 15 cm in depth By raising the air tube, the level of ... sample was assessed by a pipetting method, when 20 g of a sample are mixed with 20 ml of dispersing medium and 20 ml of distilled water, the mixture is left to stand for one day and then boiled for...
Ngày tải lên: 07/08/2014, 10:21
Báo cáo y học: "Assessment of the emotional responses produced by exposure to real food, virtual food and photographs of food in patients affected by eating disorders" ppsx
... virtual reality (VR) foods and photographs of food (PH) in two samples of patients affected, respectively, by anorexia (AN) and bulimia nervosa (BN), and in a sample of healthy controls (CTR) ... Assessment of the emotional responses produced by exposure to real food, virtual food and photographs of food in patients affected by eating disorders Annals of General Psychiatry 2010, 9:30 Page 10 of ... an effect of subjects' degree of presence experienced in the VR condition on their level of perceived anxiety (STAI-S and VASA): the higher the sense of presence, the higher the level of anxiety...
Ngày tải lên: 08/08/2014, 23:21
Synthesis, structure and magnetic properties of nanowires and films by electrodeposition
... increase our understanding of the growth mechanism of single-crystal and poly-crystal nanowires, and the coercivity mechanism of nanowires and continuous films The investigations and results are ... Alnico alloys Table 1.2: Magnetic properties of ferrites Table 1.3: Magnetic properties of Sm-Co magnets Table 1.4: Magnetic properties of NdFeB Table 1.5: Magnetic properties of L10 alloys Table ... hours), and (e) Co5(4.0 V, hours) Figure 4.7: TEM micrographs and SAED patterns of Co nanowires: (a) Bright field image of Co1, (b) SAED of (a), (c) Bright field image of Co2, and (d) SAED of (c)...
Ngày tải lên: 14/09/2015, 08:45
a discourse analysis of opening and closing speeches by masters of ceremonyon reality television showsin american english versus vietnames
... substitution is by means of “Do” “Do” operates as head of a verbal phrase in the place occupied by the lexical verb and it often located at the end of the group - Clausal substitution is by means of “so”, ... casual and loose, while formal ones are more solemn and governed by strict rules as they are meant to be used in official and serious circumstances The relation of the producer of the message and ... CONCLUSION AND IMPLICATIONS 112 5.1 CONCLUSION 112 5.1.1 The Layout of the AOSs, VOSs, ACSs and VCSs 112 5.1.2 The Influence of Culture on the Discourse of AOSs and VOSs, ACSs and VCSs...
Ngày tải lên: 26/11/2013, 12:41
USE OF HEALTH AND NURSING CARE BY THE ELDERLY pptx
... determining the extent and structure of the demand for health care and health expenditures The spectrum of diseases of the elderly is different from that of the younger population and the intensity ... subdivided by gender and age groups – of hospital admissions or discharges, length of hospital stay, contacts with doctors, long-term care-giving in institutions and at home by professional and informal ... the extent and structure of the demand for health care and health expenditures In the case of longterm care, there are other important effects that concern the structure of health care and institutional...
Ngày tải lên: 05/03/2014, 18:20
Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx
... Kroger ¨ the 5¢-UTR and 3¢-UTR of CfNR allow control of both the timing and level of expression of introduced genes in transgenic C fusiformis Results Increasing the efficiency of C fusiformis transformation ... site of pUC18 and sequenced, resulting in pUC18 ⁄ fcp1.9kb (covering 278 bp of fcp coding sequence preceded by 5¢-UTR) and pUC18 ⁄ fcp1.46kb (covering 357 bp of the fcp coding sequence followed by ... NotI and cloned into the EcoRV ⁄ NotI site of pCfcp to generate pCfcp-ble Cloning of the CfNR gene Extraction of poly(A)-rich RNA from C fusiformis and synthesis of cDNA coupled to oligo(dT)25 magnetic...
Ngày tải lên: 07/03/2014, 21:20
University musical encyclopedia the theory of music and piano technique (by e markham) (1912)
... Plan of a Musical Work and Landscape Garden Unfolding of Musical Faculty Beethoven and Shakespeare Thought and Emotion Selection of Appropriate Form CHAPTER CLASSES, CHARACTERS,, II AND KINDS OF ... which, unlike our common meter of to-day, consisted of two short lines of fours, one of six, two of fours, and one of six, with a long note at the beginning and the end of each line, thus (Ex IX) ... Variety Monotony Scientific Value of Musi Plan of a Musical Work and Landscape Unfolding of Musical Faculty Beethoven and Shakespeare Thought and Emotion Selection of Ap propriate Form Conformity...
Ngày tải lên: 15/03/2014, 13:44
Outlines of Greek and Roman Medicine, by James Sands Elliott This eBook is for the use of anyone anywhere at no cost and with almost no restrictions whatsoever. You may copy it, give it away or re-use it under the terms of docx
... consequence of the strange happening of the serpent landing from the ship the end of the island on which the Temple of Æsculapius stood was shaped into the form of the bow of a ship, and the serpent of ... father of Æsculapius, and in 460 b.c in honour of Æsculapius of Epidaurus Ten years later a pestilence raged in the city, and a temple was built in honour of the Goddess Salus By order of the Sibylline ... end of the Republic and the Beginning of the Empire Asclepiades of Prusa Themison of Laodicea Methodism Wounds of Julius Cæsar Systems of Philosophy State of the country Roman quacks Slaves and...
Ngày tải lên: 15/03/2014, 15:20
Báo cáo khoa học: Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1 pdf
... optimized by infecting Sf9 cells with different amounts of virus and by expression for various periods Aliquots of the culture supernatants and cell pellets were analyzed by SDS ⁄ PAGE and immunoblotting ... Detection of glycoproteins and deglycosylation treatment Purified E coli- and insect cell-expressed Phl p proteins were separated by SDS ⁄ PAGE and transferred to nitrocellulose followed by detection of ... comprising Zea m and Cyn d 1, a second consisting of Lol p 3, Dag g 3, Lol p 1, Phl p 1, Ory s and Tri a and a third cluster including Lol p 2, Hol l 1, Phl p and Pha a Although Phl p and Phl p are...
Ngày tải lên: 16/03/2014, 18:20
Kemetic Roots of Library and Information Science, By Itibari M. Zulu docx
... themselves and their land (Kemet: "the black land") as Black, and had others see and refer to them and their land as Black Kemetic Roots of Library and Information Science, by Itibari M Zulu Page of ... (e.g Egyptian and Wolof) between ancient Kemet and other parts of Africa The crux of the issue of race and the Egyptians is part of an attempt to take Egypt and Egyptian history out of Africa intellectually, ... "librarians" by name, e.g.: the son of Nennofre (in the House of Books and Case of Books), the grandson of King Khufu (a writer in the House of Books), Senmut, Mai, Peremhab (scribes of the archives),...
Ngày tải lên: 28/03/2014, 18:20
Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc
... elements of rmGP [4] and EcMalP [3] onto the linear sequence of StP and thus measures the extent of conservation of the respective ÔsitesÕ in StP As in other GPs, the catalytic site and the PLP ... Stability of recombinant wild-type StP and TOWER helix mutants thereof as revealed in urea and thermal denaturation experiments To compare the stabilities of StP and Arg fi Ala mutants thereof, we ... unpublished observations) Under conditions of dilute protein and in the absence of free PLP, loss of oligomer structure is accompanied by immediate release of cofactor from the StP subunit Therefore,...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo hóa học: " Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag" docx
... downregulation of other receptors is sensitive to HIV-1 Gag expression, we have now investigated the kinetics of lysosomal downregulation of CD4 and CXCR4, in the presence and absence of Gag CD4 and CXCR4 ... mutants of AIP1/Alix arrests HIV-1 budding at a late stage and blocks viral particle release [7,10] Likewise, depletion of TSG101 and other ESCRT components inhibits lysosomal downregulation of ligand-activated ... physiology and HIV-1 biology, we have examined the role of ESCRT I in downregulation of these two cellular proteins SDF-1-induced downregulation of CXCR4 and PMA-induced downregulation of CD4 were...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo khoa học nông nghiệp " Developing a strategy for enhancing the competitiveness of rural small and medium enterprises in the agro-food chain: the case of animal feed - Use of Industrial and Mixed Feed by Livestock Producers in Vietnam " doc
... brands Higher FCRs were offset by the lower price of domestic brand feeds The feasibility of establishing a cluster of animal livestock production and livestock feed producers in rural areas by ... movement of goods by 67% of small firms, 33% of medium firms and 75% of large firms Lack of capacity to undertake adequate quality control In the survey of 62 livestock feed mills conducted by CAP ... and relevant Departments of the Ministry of Agriculture and Rural Development and provincial Departments of Agriculture and Rural Development, Vietnam Association of Small and Medium Enterprises...
Ngày tải lên: 21/06/2014, 05:20
Báo cáo hóa học: " Cultivation of GMO in Germany: support of monitoring and coexistence issues by WebGIS technology" doc
... Materials and methods Open source software and standards The use of proprietary software is being determined by licences and copyrights; annual license fees may be imposed Sharing or modification of ... Federal State of Brandenburg The localisation of the GMO fields in Brandenburg was enabled by identification of the land parcels where the Bt-maize was cultivated using the ID field of the ALK listed ... the deliberate release of GMO into the environment stipulates assessment of direct and indirect effects of GMO on humans and the environment by case-specific monitoring and general surveillance...
Ngày tải lên: 21/06/2014, 06:20
The Psychology of Money and Public Finance by Günter Schmölders (Dec 12, 2006)_2 pot
... the choice of capital investment, but only above a variable threshold of property the height of which is determined by the strength of the profit motive; also by such information and habit phenomena ... effectiveness of the influence of interest rates on by students as part of their practical work: it concerns the ‘infectious’ effect produced by the acquisition by neighbours of television sets One of our ... and purchaser attitudes, of saving and property formation, of the behaviour of taxpayers and citizens, of the different influences on legislation by economic groups and single firms, we have...
Ngày tải lên: 21/06/2014, 08:20
The Psychology of Money and Public Finance by Günter Schmölders (Dec 12, 2006)_3 docx
... of thrift Our assumption is that the development of principles of thrift such as we have observed is a function of income and size of household For each combination of income level and size of ... terms of standard of living and the schedule of purchases for the households, a particular class of consumer goods played a special part at the time of the survey: the labour-saving products of ... out by a population of actors, each one acting his part as it is defined by his individual psychological make-up, by his intents, and by the prescriptions of his social role Attitudes, motives and...
Ngày tải lên: 21/06/2014, 08:20
The Psychology of Money and Public Finance by Günter Schmölders (Dec 12, 2006)_4 doc
... would fall was expected by: 55% of all respondents 61% of all men 51% of all women 64% of heads of households 64% of all male heads of households 62% of all female heads of households Yet another ... actual and the desired level of consumption, a household’s standard of consumption was defined as the sum of its level of consumption plus its level of aspiration If the two components of the standard ... labour and other means of production of those goods Secondly, the monetary authorities can bring about a change in the liquidity condition of financial institutions and of business firms and people...
Ngày tải lên: 21/06/2014, 08:20
The Psychology of Money and Public Finance by Günter Schmölders (Dec 12, 2006)_6 ppt
... special role of leadership In Schumpeter’s analysis the entrepreneur becomes the decisive ‘motor of innovation’ As such, and as the pillar of economic and technological progress and therefore of economic ... conducted by one of the leading publicopinion research institutes of Western Germany Even this latter type of research, digging down deeply into the motivational and emotional layers of the mind of ... turn of the century, a political theory of government finance, based largely on highly cynical concepts of political and administrative power.1 One of these authors, Amilcare Puviani, Professor of...
Ngày tải lên: 21/06/2014, 08:20
The Psychology of Money and Public Finance by Günter Schmölders (Dec 12, 2006)_7 pot
... justice of the rational mind, of conscience, and born of the knowledge of economic relationships – in other words, the notions of justice held by knowledgeable and responsible circles of people ... ‘higher’ level of politics and public opinion and overlook the more blunted feelings and resentments of citizens and taxpayers These attitudes and patterns of behaviour can be recorded and ‘measured’ ... independent judgement, and more of a mix of often vaguely sensed, and sometimes even contradictory feelings of justice This imbalance, and even contradiction, in the prevailing ‘conventions of justice’...
Ngày tải lên: 21/06/2014, 08:20