... concerning the kinetics and properties of the enzyme More strikingly, we show a novel role of the PPase enzyme in the development and molting process of A suum larvae in vitro constructed in UniZap ... postcultures and photographs were taken Enzyme kinetic study The Km (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard ... gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation the tissues were removed, and the larvae were collected from the bottom of the tube The...
Ngày tải lên: 20/02/2014, 11:20
... unknown, and the specific roles of the various members of the DIM family, e.g DIM A and DIM B, in these functions have never been investigated The recent developments in our understanding of the biosynthetic ... DIMs in the cell envelope raises the possibility that these compounds act in vivo by interacting with some component of the host cell and by modulating the host immune response to contain the infection ... postinfection, we observed a marked difference between the number of CFUs of the wild-type strain and that of the PMM56 mutant strain in lungs and in the spleen (Fig 7A) Indeed, both strains...
Ngày tải lên: 23/03/2014, 09:20
Báo cáo hóa học: " Targeting the inflammation in HCV-associated hepatocellular carcinoma: a role in the prevention and treatment" pptx
... (TIRdomain-containing adapter-inducing interferon-b) joining the RIG-I/MDA5 pathway In the other pathway, TLR7 senses single-strand HCV RNA and via the MyD88 adaptor protein activates IRAK4/IRAK1 These kinases stimulate ... exceeds the system’s ability to neutralize and eliminate them In these conditions, ROS and RNS affect the intracellular and intercellular homeostasis, leading to possible cell death and regeneration ... examined in HCC by in vitro and in vivo studies, both in animal models and in humans In particular, from some studies, conducted both in vivo and in vitro, resveratrol emerged as a promising...
Ngày tải lên: 18/06/2014, 16:20
The Fungal Community Its Organization and Role in the Ecosystem Third Edition docx
... fungi We will see in the following chapters immense diversity in the functioning of fungi, their associations with other organisms in the community, and their role in regulating ecosystem processes ... determine their abundance, determine their associations among themselves and other organisms, measure their individual and community function, and be able to scale these measures from the microscopic ... trademarks, and are used only for identification and explanation without intent to infringe Library of Congress Cataloging -in- Publication Data The Fungal community : its organization and role in the ecosystem...
Ngày tải lên: 23/03/2014, 01:20
Báo cáo khoa học: Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides pptx
... processes of the assembled PufX protein The exchange of ubiquinone between the RC and the cyt bc1 in the presence of mutated PufX protein The role of the PufX protein in facilitating the ubiquinone/ ... hydrophobic interactions are involved in maintaining the dimeric form The data obtained on the PufXD2)7 and PufXD2)19 strains indicate that important protein–protein hydrophobic interactions ... demonstrated the role of the C-terminal amino-acid residues of the LH1 a polypeptide in the organization of the LH1–RC complex [12] In the present work, we have investigated the possible involvement of the...
Ngày tải lên: 31/03/2014, 09:20
Tài liệu The Role of BCG Vaccine in the Prevention and Control of Tuberculosis in the United States: A Joint Statement by the Advisory Council for the Elimination of Tuberculosis and the Advisory Committee on Immunization Practices docx
... Control and Prevention The role of BCG vaccine in the prevention and control of tuberculosis in the United States: a joint statement by the Advisory Council for the Elimination of Tuberculosis and the ... adults These studies also were not useful in determining a) the efficacy of BCG vaccine in HCWs or b) the effects on efficacy of the vaccine strain administered Vol 45 / No RR-4 MMWR and the vaccinee’s ... vaccination of adults and the type of vaccine strain administered precluded the inclusion of these factors as covariates in the random-effects regression model However, these researchers determined...
Ngày tải lên: 15/02/2014, 13:20
Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf
... sequence for the interaction with the PDZ proteins: Mint-3, AMPA binding protein and glutamate receptor interacting protein (GRIP) It would therefore be interesting to determine whether this motif ... fusion to the GAL4 DNA binding domain We therefore cannot rule out an interaction between MT1-MMP ICD and the GRASP55 PDZ1 domain GRASP55 binds to furin, PC5/6B and PC7 intracellular domains The pro-convertase ... GRASP55 binding site in the ICD of MT1-MMP, as well as the GRASP55 domains involved in the interaction with MT1-MMP ICD We also describe the GRASP55 interaction with the furin ICD, and provide...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... of the aglycone-binding site in the stabilization of ESà and the interdependence between the binding of aglycone and the positioning of glycone in subsite )1 are not known In this study, the role ... was further investigated by determining the in uence on the glycone binding of the Sfbgly interactions with the aglycone Thus, the effect of the alteration of the glycone structure in the stability ... E190 and E194 are positioned in an internal region of the aglycone-binding site, probably subsite +1, because they in uence the binding of the first glucose unit of the aglycone On the other hand,...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although there are four histidines in the C-terminal, another ... partitioning of the protein into an aggregated state The effect of copper binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein ... Copper binds to cystatin B change but lacks any copper binding capacity This point mutation lies within the C-terminal region and therefore could affect the structure of this part of the protein However,...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... and containing the galA gene and approximately 700 bp 3¢-flanking region, in pIM4700 (containing the pkiA promoter) A niger The sugar composition of the arabinogalactans from potato, onion and soy ... proteins The three arabinogalactans were incubated with GALA for 15 min, 30 min, h and 20 h to determine the substrate specificity of the enzyme The degradation of the polymer Ó FEBS 2002 and subsequent ... proteins in A niger [33] The fusion construct (pIM3982) was made by introducing an NsiI site at the translation start point of galA and by then cloning a fragment, starting at this NsiI site and...
Ngày tải lên: 21/02/2014, 01:21
HUMAN PERFORMANCE: Role of General Mental Ability in Industrial, Work, and Organizational Psychology docx
... use of objects (“things”) and machines, independ- 31 Processing information (perceiving, retrieving, manipulating, transmitting) Compiling information, importance of Combining information, importance ... personality, and preferences (i.e., values, interests) combine and interact with facets of the environment to determine both intraindividual and interindividual differences in the acquisition of domain-specific ... slips; understanding news articles and insurance options; and the like) and health literacy (understanding doctors’ instructions and medicine labels, taking medication correctly, and so on) reflect,...
Ngày tải lên: 06/03/2014, 05:20
Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... provide insight into the receptor specificity of the B2wt internalization motif The resulting data also hint at a receptor specific role of the putative helix in G protein activation and interaction ... Role of helix and C-termini in bradykinin receptors G protein uncoupling are essential Some of these desensitization mechanisms involve the translocation of the stimulated receptor to distinct ... in percentage of total binding, decreasing the binding affinity of the FEBS Journal 272 (2005) 129–140 ª 2004 FEBS A Faussner et al Role of helix and C-termini in bradykinin receptors Fig Schematic...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... VBARP and Vpr is specific The input panel represents the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction ... measuring 1881 bp (designated VBARP-L) and a second 1305 bp clone (designated VBARP-S), were constructed and used to further confirm the Vpr and VBARP interaction using in vitro and in vivo interaction ... ankyrin repeat proteins, using the consensus established by Kohl et al [10] and Mosavi et al [11], which contains ankyrin repeats from over 4000 proteins, indicated that the ankyrin repeat domains...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... subsite Substrate binding induces conformational changes involving the ArgA145PheA146 fragment of the protein chain, acting as a flap, first opening and then closing the groove [45] The catalytic reaction ... ArgA145 and ArgB263, on the negatively charged COO– in the aminic part determines the position of the substrate within the S1¢-binding subsite O atoms of the main chain CO groups of LeuB387 and TrpB240, ... of the side chain d-guanidino group of this residue are involved in H-bonding as follows: Ne with the O atom of the main chain CO group of the TrpB240; Ng1 shares H bonding with O atom of the...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx
... of the various bands, the proteins were extracted from the native gel and analysed by SDS/PAGE (Fig 1B) Indeed both proteins form a complex and run together as they are present in the same band ... with a binding constant Kd of 2.75 lM, and that the amino-terminal domains of the two peroxins were responsible for this interaction The role of TbPEX14 in glycosome biogenesis received further ... was located in the band This may be due to diffusion of some complexed protein as the band migrates in the native gel Band contains the excess of TbPEX14-N,H In a second experiment, the same two...
Ngày tải lên: 23/03/2014, 17:21
Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx
... kU0 and the Gibbs energy of unfolding at [urea] (DGU0) In the mutant series, the overall height of the energy barrier for the rate-determining step of the folding ⁄ unfolding transition is mainly ... on the structural model from [35] along the N-terminal a helix involving the amino acids V4 ⁄ N5, and S11 ⁄ A12 The side chain of D72 is part of an acidic patch, together with the residues D2 and ... along the C-terminal a helix to E68 and Y76 Interestingly, one of the side chain oxygens of D72 appears to be hydrogen-bonded to the backbone N atom of G6, bridging the crossing between the N- and...
Ngày tải lên: 30/03/2014, 20:20
The Reverse Logistics Process in the Supply Chain and Managing Its Implementation
... both the firm and the customers (Stock and Mulki 2009) Increasing the understanding of the factors related to reverse logistics and product returns can assist in identifying areas in supply chain ... entities within the chain to enhance the process Research is still determining and testing the factors that impact the process and may not be to the point of being able to establish incentives ... with the key factors perceived in industry Now that the literature has been thoroughly reviewed and assessed against the framework of Carther and Ellram (1998), obtaining and analyzing the inputs...
Ngày tải lên: 01/06/2014, 14:04
Play and it’s role in the mental development of the child
... dominating to a subordinate position 17 Thus, in play the child creates the structure meaning/object, in which the semantic aspect – the meaning of the word, the meaning of the thing – dominates ... in the form of other things But the moment the stick – i.e., the thing – becomes the pivot for severing the meaning of “horse” from a real horse, the child makes one thing influence another in ... examining a piece of candy, putting it in one’s mouth, chewing it, and then spitting it out In play the object, to win, is recognized in advance At the end of play development, rules emerge; and...
Ngày tải lên: 23/06/2014, 09:52
Báo cáo khoa học: "The role of photoperiod and temperature in the induction and the release of dormancy in Pinus sylvestris L. seedlings" potx
... fulfill the chilling requirement to break winter dormancy in Scots pine has been stressed (e.g., Wareing, 1951; Vegis, 1965; Sarvas, 1974) In the following,I will use the working definition of dormancy ... at the start of night prolongation, or 12 wk, an important role in the possibility of the buds to attain a deeper stage of dormancy Only the 12 wk old plants of northern origin had no flushing ... (Fig 2) The same was true for the higher temperature 25/15°C compared with 25/5°C Dormancy breaking treatments of and wk had a dramatic influence, hastening the tilushing rate, especially in the...
Ngày tải lên: 09/08/2014, 02:21
Báo cáo y học: "γ The role of the complement and the FcγR system in the pathogenesis of arthritis" pdf
... [20] The complement receptor CR1 binds the C3b, iC3b and C4b fragments, CR2 binds the C3d and iC3b fragments, and CR3 binds the iC3b fragments These interactions have been implicated in the immune ... at the same time and, for B cells, on the very same cell Both FcRs and complement receptors are involved in the binding of ICs and phagocytosis; they deliver signals leading to the activation and ... complement or FcγRs in the inflammatory response, especially in Arthus reaction mice models, have resulted in a better understanding of the role of the complement system and FcγRs, although the relative...
Ngày tải lên: 09/08/2014, 06:23
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