importance of tourism industry and its role in the philippine economy

... research is that understanding what causes the loss of appropriate metal binding might be crucial for the understanding of the role of amyloidogenic proteins in a number of neurodegenerative disorders. Experimental ... with glycine alone was performed and no binding was observed. In our hands, as others [54], the most reproducible data was obtained from injections of the metal into a solution of the protein. Typically ... important in the brain, there- fore the role of copper binding or loss of its binding could be related to specific cerebellar function(s) of stefin B [18], which remains to be seen by more in vivo studies. Stefin...

Ngày tải lên: 19/02/2014, 06:20

... concerning the kinetics and properties of the enzyme. More strikingly, we show a novel role of the PPase enzyme in the development and molting process of A. suum larvae in vitro. Materials and methods Parasites Adult ... 14.635 lmolặmin )1 ặmg protein )1 . The inset in (A) repre- sents the linear transformation of the curve. (B) Mg 2+ dependency was determined as described in (A) in the presence of increasing concen- trations ... determined by incubating the diluted recombin- ant proteins in the standard reaction mixture in the presence of increasing concentrations of PP i (0.05–0.5 m M )at55°C. Data were fit to the appropriate...

Ngày tải lên: 20/02/2014, 11:20

... mislocalization of all three enzymes in these cells clearly shows the involvement of PEX14 in the targeting of PTS-1, PTS-2 and I-PTS proteins to the glycosome. To further analyse the effect of the destruction ... a binding constant K d of 2.75 l M , and that the amino-terminal domains of the two peroxins were respon- sible for this interaction. The role of TbPEX14 in glycosome biogenesis received further ... the peroxin PEX14 is a component of a docking complex at the peroxisomal membrane, where it is involved in the import of matrix proteins into the organelle after their synthesis in the cytosol and...

Ngày tải lên: 23/03/2014, 17:21

Ngày tải lên: 13/08/2014, 09:20

... v-KIND KIND2 binding resides within residues 702–744 of MAP2. Residues 456–487 in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within the KIND2 ... specific MAP2 binding resides within the 32 residues (amino acids 456–487) of the v-KIND KIND2 domain. To examine whether the MAP2 binding core site of v-KIND binds to intact MAP2 protein, we coex- pressed ... under- stood, the data obtained in the present study indicate that the 43 residues (amino acids 702–744 in mice) that reside in the middle region of the CD act as the v-KIND binding core. The v-KIND-binding...

Ngày tải lên: 14/02/2014, 19:20

... the basis of the above findings, our research provides new insights into the role of exon 9 in the regulation of apoptosis and invasion in liver cell lines. A B Fig. 7. Effects of occludin shRNA ... important roles in the induction of mitochondria-mediated apoptosis and the inhibition of invasion, along with the downregulation of matrix metalloproteinase expression. In addition, by using the calcium ... Matrigel-coated inserts were stained with crystal violet. The dots are stained cells on the bottom of the Matrigel-coated inserts. The number of invasive cells was determined by counting the number of stained...

Ngày tải lên: 18/02/2014, 18:20

... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU bind- ing homolog of enoyl-CoA hydratase’). The RNA- binding activity of the human protein ... For the kinetic characterization of AUH described in the work at hand, AUH was overproduced in Escherichia coli as a maltose binding protein fusion (MBP-AUH). The complete AUH gene (producing ... gặL )1 bovine serum albumin and 10 lm 3-MG-CoA or glutaconyl-CoA. After incubation at 37 °C for 60 min, the reaction was terminated by the addi- tion of 2.5 lLof2m HCl. The samples were homogenized, and...

Ngày tải lên: 19/02/2014, 07:20

... that the interaction between VBARP and Vpr is specific. The input panel represents the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and ... con- firmed that the cytoplasmic distribution of VBARP is independent of the cycling stage of the cells. Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous ... protein containing a single ankyrin repeat that interacts with HIV-1 viral protein R (Vpr) and we designated this protein as Vpr-binding ankyrin repeat protein (VBARP). This interaction was further...

Ngày tải lên: 07/03/2014, 21:20

Ngày tải lên: 12/08/2014, 18:20

Ngày tải lên: 13/08/2014, 11:22

Ngày tải lên: 24/08/2014, 10:58

Ngày tải lên: 24/08/2014, 12:32

Ngày tải lên: 05/09/2013, 10:17

... “hated”… At the beginning of the story, the main character was introduced “an old Indian named Koskoosh” and the story is told from the point of view of this character. In the text, one can find many ... a randomly chosen text in terms of expansion to see how the findings can help to understand some features of the text and the intention of the writer. In this thesis, the text chosen is the ... realization in the short story The Law of Life by Jack London has been finished. Now we shall sum up the results of the study. In chapter 2 of the study, the theoretical background of the most...

Ngày tải lên: 29/01/2014, 14:44

... nm, comparable to the inhibition of the aminopeptidase cathepsin H (K i ẳ 30 nm ). In comparison with other cystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized ... Cystatins are generally rather non- selective inhibitors. An interesting feature of cystatin F is the 100-fold stronger inhibition of cathepsins F, L, and V than of cathepsin S. Cathepsin S is ... process regulating its inhibitory properties. Screening of proteases for their inhibition showed that cystatin F is different from other cystatins, both in terms of specificity and strength of binding to the target...

Ngày tải lên: 19/02/2014, 17:20

... Prx class, is their second redox-active cysteine, the resol- ving cysteine (Cys-S R ). In typical 2-Cys, S p at the N terminus and S R at the C terminus of the protein belong to different subunits and ... periods of time (Fig. 3). As shown, the addition of stressors to the cul- tures inhibits growth without killing the cells. The hybridizing band in the northern analysis showed the expected size of ... processes. In this study we examined the role of Bcp2 in order to increase the knowledge of the enzymatic activity involved in the oxidative stress in S. solfataricus. To detect differences in the response...

Ngày tải lên: 07/03/2014, 12:20

... 20 lL of the enzyme/inhibitor mixture to 180 lLof substrate (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP. Protein assays and protein sequencing Total protein in crude ... property of enzymes involved in transformation of xenobiotics is increased enzyme levels in the presence of xenobiotic substrates, i.e. inducibility. Cloning the human cbg- 1 gene (i.e. including the ... mLámin )1 .The following gradients were used: incubations containing arbutin or salicin as substrate; 100% C initial, i ncreasing D t o 2 0% (10 min), 50% ( 15 min), 100% (5 min), held at 100% (5 min);...

Ngày tải lên: 08/03/2014, 16:20

... its charge in the broadest sense and to examine both the fundamental roles of solar and space physics as aspects of scientific exploration and the roles of the research in support of enabling ... are defined broadly and that include exploration to understand the origin and evolution of the universe, the formation of planets and planetary systems, the origin and extent of life, and the ... element of the fate theme, and it contributes in key ways to many aspects of several components of the origins and evolution themes. In light of the commission’s findings, the Committee on the...

Ngày tải lên: 14/03/2014, 10:20

... only 10% of urate is excreted in the urine (the remaining 90% being recirculated by the renal system [3]), an increase of this antioxidant in blood will also lead to an increase in urine. The fact ... H, Savant Instruments Inc.). The final volume was deter- mined by gravimetry, assuming that the entire methanol was removed during the conc entration in the SpeedVac and that the d ensity of the remaining ... (Institute for Work and Health) for their help in the metal/creatinine analysis, and comments on the manuscript. The medical team of the Institute for Work and Health (Prof Marcel-André Boillat, Dr...

Ngày tải lên: 20/06/2014, 00:20

... of rice and to investigate the effect of tempering on the increase in the mechanical strength of rice. This was the preliminary work mainly to establish the methodology and feasibility of the ... extension workers, and collection of more da training part of the program in the next 3 m 24 shared the existing information and experiences on rice cracking. The document prepared (in Vietnamese ... 10: Increased mechanical strength of the rice due to tempering 22 1. Institute Information Project Name Investigation of rice kernel cracking and its control in the field and during...

Ngày tải lên: 21/06/2014, 06:20