... great-grandmother Grandma, b. 1901 grandmother/homemaker Gram, b. 1903 grandmother/homemaker Grandad, b. 1903 grandfather/bookkeeper Grandpa, b. 1911 grandfather/doctor Fritz Markley, b. 1929 father-in-law/locksmith ... original family Al, b. 1959 brother/lawyer Dad, b. 1934 father/IT professional Doug, b. 1962 brother/lawyer Drew, b. 1970 dog Mom, b. 1936 mother/writer Sam, b. 1960 brother/artist Chicago circle ... Marco Torez security officer Matt Benjamin IT professional Matthew Martinez supervisor Melanie Bricker office administrator Michelle Jennings office administrator Nikos IT professional Odin...
... to the catalytic site ofthe R1E subunit. Asreported in the Results, upon completion of substrateconversion, the radical is then rapidly passed backfrom the R1E subunit to the tyrosyl ofthe ... RNR and that of other class 1RNRs or, instead, is a result ofthe interaction of the R2F with the inactivated (oxidized) form ofthe R1E.We consider the first option unlikely. Under these cir-cumstances, ... recipient.In the present study, we report data on homologous expression ofthe nrdF gene of C. ammoniagenes strainATCC 6872. This is the first report ofthe successfulpurification of high amounts of the...
... from that ofthe oviduct ecto-ATPDase.Immunochemical staining demonstrates the distribution of the ecto-ATPDase in the bile canaliculi ofthe chicken liver.HeLa cells transfected with the chicken ... Besides oviduct and liver, the chicken ecto-ATPDase is also present in the apical membranes of the oxyntic-peptic cells [37]. The distribution ofthe ecto-ATPDase on these epithelial cells is distinctly ... different fromtheotherATPDaseintheE-ATPasefamily,theCD39s[13,17,19].Molecular cloning of chicken liver ecto-ATPDase The results described above indicate that: (a) the enzymaticproperties ofthe chicken...
... [Pg 1] The Southof France. I. known, the gifts of all property that were made to the Church, the abandonment of worldly pursuits, the terrors of many, the anxiety ofthe calmest, the emotional ... telling ofthe Cathedrals of the South which was at once accurate and complete. For the Cathedrals of that country are monuments not only of architecture and its history, but ofthe history of peoples, ... ancients. To Virgil the adventures ofthe “pious Æneas” were truly heroic. The western shores ofthe Mediterranean were then the “end ofthe earth,” and even during the first centuries of our own era,...
... Some of the methodologies employed and the basic results for these other countries are discussed by Mani (2007). However, it is not the intention of this report to analyse the results of these ... 3.2% ofthe turnover of these enterprises. In both the industrial and services sector, the bulk of innovation expenditure was devoted to the acquisition of new machinery, equipment and software, ... on benchmarking the results oftheSouth African Innovation Survey with the results of CIS4 undertaken in the various EU countries (as well as Norway and Iceland). The results of innovation surveys...
... processing of the D. melanogastera-F1-ATPasetranscript The expressionofthe a-F1-ATPase gene during develop-ment is coordinated with theexpressionofthe nuclear-encoded b-F1-ATPase gene and the ... under the control ofthe actin 5Cpromoter. The GAGA factor stimulated at least threefold the activity ofthe promoter in constructs )397/+86 and)146/+86, but had no effect on the activity of the construct ... similar activity in Schneider cells to the promoter of the b-F1-ATPase gene [34] and 10-fold stronger than the promoter ofthe gene encoding the catalytic subunit of the mitochondrial DNA polymerase...
... clearlygrouped the trout IL-11 with IL-11 molecules fromother species and separate from other members of the IL-6 family.In vivo expressionof IL-11RT–PCR was used to examine theexpressionof troutIL-11 ... differ-ences were a 26 bp insertion in the 5¢-UTR of the cDNA and an insertion of 12 repeats with a consensus of CCAATGATGATCCAAGAAATCCACACTACAG(31 bp) in the 3¢-UTR ofthe cDNA sequence (Fig. 1).A ... in the 5¢-UTR, and four potentialpoly(A) signals were found in the 3¢-UTR (Fig. 1), two of them just 14 or 23 bp upstream ofthe poly(A) tail. The remaining two poly(A) signals were upstream of the...
... gene for one ofthe proteolipid subunits of V-ATPase. Expressionofthe cDNAs in the strain revealedthat four cDNAs from the six complemented the protontransport activity into the vacuole, ... progress.Isoforms of V-ATPase subunits have so far been reportedin higher plants as reviewed by Sze et al. [1], three isoforms of the subunit a of mouse enzyme [22,23], four isoforms of the proteolipid ... isoforms as observedin higher plants V-ATPase. We have also isolated twodifferent cDNAs coding for the subunits A and B of V-ATPase. The intracellular localization of these isoformsand the...
... questionnaires. Many of the smaller firms did not see the relevance ofthe Innovation Survey to their businesses. Because ofthe relatively low response rate to the survey, some ofthe smaller sub-strata ... Some of the methodologies employed and the basic results for these other countries are discussed by Mani (2007). However, it is not the intention of this report to analyse the results of these ... turnover of all enterprises and 2.1% ofthe turnover of innovative enterprises. Intramural and outsourced R&D accounted for 0.69% ofthe turnover of all enterprises and 1% ofthe turnover of...
... them out, take the blacking and brush, and go at them." CHAPTER IV THE BOAT-RACE. At eight o'clock, on the evening ofthe third day ofthe passage, the lights of another steamer ... Just at the edge ofthe city, and sheltered by large poplar-trees was the old homestead in which she resided. There was a splendid orchard in the rear ofthe house, and the old weather-beaten ... citizen of the first standing among the whites, but even the slaves regarded him as one ofthe kindest of masters. Having inherited his slaves with the rest of his property, he became possessed of...
... suggesting that the level of GFP–PrPCtransgene expression was dependenton the colour ofthe background ofthe animal. The fusion protein was found only in the NIL and not in the AL of black- and ... investigate the intracellular fate of PrPCby examining for the firsttime its biosynthesis in the secretory pathway of neuro-endocrine cells in vivo and the effect ofthe transgene expression of PrPCon ... approach with the intermediatepituitary melanotrope cells ofthe South- African claw-toed frog Xenopus laevis. Depending on the colour of the background ofthe animal (black or white), thesecells...
... present in the othermembers ofthe APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of a secondheparin-binding domain [3,4,13,26].Comparative analysis of the Xenopusand ... andmammalianAPLP2.Overall, the high degree of conservation of APLP2 mayhelp the identification of functionally important domainswithin this APP superfamily member. Expression pattern of XenopusAPLP2 mRNA The expression ... Xenopus .The availability ofthe Xenopus APLP2 protein sequenceallowed a phylogenetic analysis of APP superfamily mem-bers that suggested the occurrence of APP and preAPLPlineages with their...
... may haveacted on the level of pyrG expression has been removed. The strength ofthe feedback regulation of CTP on pyrG expression, i.e. the elasticity of pyrG expression for the CTP concentration ... pyrG expressionof up to 250% ofthe wild-typelevel (Fig. 4A). The results show that the feedbackinhibition ofthe CTP synthase enzyme is incompletein vivo. In conclusion, the homeostasis ofthe ... downstream of synthetic promoters. Expression from pyrG in the wildtype is regulated by the concentration of CTP in the cellby an attenuation mechanism in the 5¢-end ofthe pyrGmRNA [6]. The primer...
... particlesfrom grass pollens [10,11]. The small size of these sub-cellular particles allows them to reach the deeper air-ways and may explain the frequent occurrence of heavy asthma attacks after rainfalls ... Spectrometer (ThermoQuest Inc.). The spectra were deconvoluted using Thermo Finnigan’s xcali-bur software and the spectra were also verified by hand cal-culations of charge states. The proteolytic ... preincubation of patients’ sera withsmall recombinant protein fragments suggesting the importance of conformational IgE epitopes [25].Therefore, we further tested the importance of struc-tural...