... they were isolated had an inhibitory effect [7]. The number of mature liver schizonts increased in the presence of mAbs anti-CSP of P. yoelii and the authors suggested that enhancement of ... important role in the control of malaria infection. However, they can modulate parasite development enhancing infection. The effect of anti-Plasmodium antibodies on theexpressionof circumsporozoite ... This study sets to understand the role of anti-Plasmodium antibodies on the erythrocytic development of Plasmodium falciparum and their effect on theexpressionof csp gene. 2. Materials and...
... inhibition of proliferation of VSMC, which ismechanistically linked to a KLF4-induced enhance-ment oftheexpressionofthe tumour suppressor genep53 [5]. Because ofthe important roles of KLF4 on the ... found that the promoter of SR-BI contained multiple putative KLF4binding sites. However, the direct effect of KLF4 on the expressionof SR-BI remains unknown.Here, theexpressionof KLF4 in ... important role in the activation of endothelialcells and macrophages, as well as the differentiationand proliferation of VSMCs. Overexpression of KLF4induced expressionof multiple anti-inflammatory...
... degrada-tion in the population of hemoglobin-positive cells.To the best of our knowledge, there has been noreport on the promoter function ofthe human HO-2Fig. 7. Characterization ofthe human ... Takentogether, these results indicate that hypoxia reduces the expression levels of HO-2 mRNA and protein infive out of six cell lines, with the exception of KG1myeloid cells.Hypoxia decreases theexpression ... address the question of whether hypoxia influences the promoter activity ofthe human HO-1 or HO-2gene, we performed transient expression assays. Priorto the functional analysis ofthe HO-2 gene promoter,we...
... way [19]. When comparing the MLC region ofthe 2D gels between TA from PV– ⁄ –and PV+ ⁄ + mice, the pattern is virtually identicalwith the exception ofthe lack ofthe PV spot in PV– ⁄ –mice ... regions of the PV and MLC3F gene has been previouslyobserved. A segment of 32 bp was identical in bothgenes and, based on these findings, the authors pro-posed that theexpressionof PV and ... levels of MLC3 and PVare much higher in TA than in SOL, the ratio of the two proteins in each ofthe two muscles seemed to beconstant (Fig. 2C). A high degree of homologybetween short segments of...
... arelocalized on different chromosomes the question arisesconcerning the mechanisms underlying the orchestrated expression of these enzymes by the oxygen tension. The findings that the effect of ... twofold increase of PDI mRNA,a more moderate increase ofthe mRNAs for prolylhydroxylases, and did not increase the mRNA abundance of the other enzymes.DiscussionOur data show that theexpression ... hypoxiathrough the UPR [40] induced by cellular energy depletion[41,42]. We have addressed this issue therefore by investi-gating the influence ofthe UPR for theexpressionof the procollagen...
... and alters theexpressionofthe twonuclear genome coded isoforms of CytOX V [10,32]. The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... study therefore, we investigated how O2modulates the expressionof mRNAs for CytOX subunits and also the catalytic activity ofthe enzyme complex. Our results showthat the levels of some ofthe ... 2003ResultsAs a measure ofthe hypoxic effect on CytOX gene expression, we measured the steady state CytOX mRNAlevels. The Northern blot in Fig. 1 shows the effect of hypoxia on the levels of mitochondrial...
... [34–36],might regulate theexpressionof Ppp2r5c mRNA priorto the appearance of Nrl in the retina. Like the increase in Nrl expression during development,Ppp2r5c mRNA expression was precisely ... To further examinewhether the increased expressionof Ppp2r5c transcriptswas specifically modulated by Nrl, we used Nrl smallinterfering RNA (siRNA) transfectants in which the expressionof Nrl ... &DePaoli-Roach AA (1996) High complexity in the expressionofthe B¢ subunit of protein phospha-tase 2A0. Evidence for the existence of at least sevennovel isoforms. J Biol Chem 271, 2578–2588.9...
... effects on theexpressionof 12 genesand opposite effects on the regulation of eight genes.These results suggest that rapamycin inhibition of TORC1 modifies theexpressionof a set of genes thatonly ... from the intestinal digestion of proteins [4,5].Another example ofthe detection ofthe lack of anamino acid is metabolic adaptation to cope with epi-sodes of protein malnutrition. In these ... in bold). The enrichment for a given GO category was computed as the ratio of the distribution ofthe amino acid-regulated genes* versus the distribution ofthe genes spotted onto the microarray**....
... steroidsynthesis or the reduction in arachidonic acid releasemay regulate theexpressionof ACS4. The human gene encoding ACS4 is located in the chromosome Xq 22–23 region close to the a5 chain of the type ... mitochondria). The reason why the action ofthe two oppositeenzymes, namely the arachidonoyl-CoA synthetase and the arachidonoyl-CoA thioesterase, is needed may be the need to sequester AA from the free ... pro-duction (the major steroid produced by the MA-10 cellline) and correlated the steroid-producing capabilitywith the level of protein expression as assessed bywestern blot. Theexpressionof MTE-I...
... that the inhibition of CDK activityincreases theexpressionof Rb and Sp1.Fig. 1. Effects of UCN-01 on the levels of Rb and Sp1 protein. (A), (B) Dose–response ofthe effect of UCN-01 on the levels ... However, there are also somedifferences, namely (a) in the case of Rb, there is an increasein both the levels of mRNA and the stability ofthe protein,while the degradation rate of Sp1 does ... annealing of primers to nontarget DNA. The lower solution contained the cDNA, the MgCl2, the dNTPs, the [32P]dATP[aP] and one half ofthe buffer, and the uppersolution contained the four...
... occursin the nuclei and the tail during the maturation process in the epididymis. The greater the extent of oxidation in nuclei, the more potent are the spermatozoa. In addition, hyper-activation of ... it to the reduced form (GSH) usingan NADPH as the electron source. The function of GR in the male genital tract ofthe rat was examined by meas-uring its enzymatic activity and examining the ... enzymatic activity and examining the gene expression and localization ofthe protein. Levels of GRactivity, the protein, and the corresponding mRNA were the highest in epididymis among testes,...
... On the other hand, in HeLa cells, it was reportedthat theexpressionofthe full length TRAF1 potentiated the activation of NF-jB induced by TNFa or IL-1, butthat ofthe C-terminal part of ... (Affymetrix) and the levels ofthe expression of each cDNA and ofthe reliability (Present/Absent call)were calculated with software (AFFYMETRIX MICROARRAYSUITEver.4.0). For comparisons oftheexpression ... responses.We also examined theexpressionof TRAF1 and TRAF2because they interact and regulate the function ofthe 4-1BBproteins to induce NF-jB [18]. In the absence of T3, neitherHeLa nor HeLaTR...
... imply the involvement ofthe O-glycosylation of Sp1 in the induction of SREBP-1a transcription byglutamine. The gene expressionof SREBP-1c, whichrepresented less than 20% ofthe SREBP-1a gene expression ... increase in the gene expression of SREBPs, and second, the enhancement of SREBPprocessing by glutamine. We therefore investigated the effect of glutamine on the gene expressionof SREBPfamily members, ... essential for the constituent of membrane, we examined whether gluta-mine regulates theexpressionof genes involved in cho-lesterol homeostasis. To investigate the effect of glutamine on the transcription...
... upon the peer review comments, the best practice statement was submitted to and approved by the PGC and the Board of Directors ofthe AUA. Funding ofthe Panel and ofthe PGC was provided by the ... Cryosurgery for the Treatment of Localized Prostate Cancer The supporting systematic literature review and the drafting of this document were conducted by the Cryosurgery for Treatment of Localized ... imaging, the edge ofthe frozen tissue appears as a hyperechoic rim with acoustic shadowing.22,24 The use of thermocouple devices introduced in the mid 1990s allowed the surgeon to determine the...
... as the overexpression of Q2-derived DM isoforms could be attributed to the overexpression of TAp73, which is known to activate the P2 promoter [13]. In this case, downregulation of DMp73 expression ... processes.Therefore, all of these issues should be addressed in the future.Taken together, our findings make it clear that thereis a link between theexpressionof Sp1 and p73isoforms in lung cancer. ... cellsAs the outcome ofthe action of TAp73 is dependenton the presence ofthe dominant negative DMp73 [13],an important issue to be considered is whether Sp1also affects DMp73 levels in the context...