k channel subunits in cultured cell lines

... Human cell lines used were HeLa cervical carcinoma cells, HepG2 hepatoma cells, K5 62 and YN-1 erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung cancer cells, ... HepG2 cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma) YN-1 cells were maintained in Iscove’s modified Dulbecco’s medium (Sigma), and K5 62, KG1 and Jurkat cells were maintained in ... melanoma cells H146 small cell lung cancer cells were obtained from ATCC (HTB-173) and cultured in RPMI-1640 medium HMV-II melanoma cells were obtained from Riken Cell Bank and cultured in nutrient...

Ngày tải lên: 19/02/2014, 05:20

... N3 9K cystatin C, lacks inhibitory activity against the C13 family cysteine protease, legumain, because of removal of the key amino acid in the legumain-binding site of cystatin C [22] The amino ... the cysteine inhibitory capacity of cells The human cancer cell lines chosen included one epidermoid carcinoma cell line, three mammary tumour cell lines and one human pancreas adenocarcinoma All ... convincingly demonstrate that the cysteine protease inhibitor cystatin C is internalized in all five of the cancer cell lines investigated in this study Using confocal microscopy, western blotting...

Ngày tải lên: 16/03/2014, 06:20

... [10–12] In addition, ROS derived from leukocytes induce excessive in ammation, leading to cell and tissue damage [13,14] In extreme instances, such as endotoxin shock, neutrophils kill the infected ... cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro Results O2–-scavenging ... 333–335 Koketsu M, Okayama Y, Aoki H & Ishihara H (2002) Facile synthesis of N,N-dialkylselenoamides from amides Heteroatom Chem 13, 195–198 Takahashi H, Nishina A, Fukumoto RH, Kimura H, Koketsu...

Ngày tải lên: 23/03/2014, 07:20

... doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression of the original TetR instead ... of human cell lines; primate and canine cell lines may also be used [5] It has been extensively documented that the plasmids are maintained episomally (5–10 copies per cell, e.g for 293 cells), ... results for pEBTetD cell pools in continuous culture up to 10 weeks indicate useful factors of inducibility on the level of protein function Clearly, in many situations, e.g in RNA interference experiments,...

Ngày tải lên: 23/03/2014, 09:21

... specific VRK2B message (dotted lines) in the H1299 (blue) and MCF7 (pink) cell lines (D) Localization of endogenous VRK1 and VRK2 proteins in three adenocarcinoma cell lines VRK1 was detected with a ... amounts in the remaining carcinoma cell lines (Fig 3A) In lymphoma cell lines, only isoform A was detected To identify the mobility of each protein, protein extract of MCF7 cells was run in parallel ... threonine 18 through a cascade mediated by a casein 1-like kinase Effect on mdm2 binding J Biol Chem 275, 9278–9283 50 Dumaz N, Milne DM & Meek DW (1999) Protein kinase CK1 is a p53-threonine 18 kinase...

Ngày tải lên: 30/03/2014, 11:20

... dasatinib in combination with chemotherapy was examined in the three dasatinib responsive cell lines, Lox-IMVI, HT144 and Malme-3M and in one of the dasatinib-resistant cell lines, Sk-Mel-28 In ... dasatinib Discussion We have evaluated the effects of dasatinib, a multi-targeted tyrosine kinase inhibitor, in human melanoma cell lines [6] In a previous study in breast cancer cell lines, ... sensitivity biomarker [28] Interestingly EphA2 levels were significantly higher in the three dasatinib sensitive cell lines than in the two resistant cell lines Although the number of cell lines is small,...

Ngày tải lên: 18/06/2014, 15:20

... Medicine 2011, 9:64 http://www.translational-medicine.com/content/9/1/64 Page of 10 Figure SU11274 blocked motility in RMS cell lines A, SU11274 inhibited wound healing in RMS cell lines RMS cells ... chemiluminescence; EGFR: epidermal growth factor receptor; IGF-IR: insulin growth factor-I receptor; PI 3K: phosphoinositide-3-kinase; MAPK: mitogen activated protein kinase Acknowledgements We thank ... block c-Met function in RMS cell lines and normal muscle cell line, HASMC In CW9019 and RH30 cell lines, which expressed high levels of phospho-c-Met, the IC50 of SU11274 was 2.5 μM; whereas in...

Ngày tải lên: 18/06/2014, 19:20

... the 11 BRAFV600E mutant cell lines, in cell lines the combination was antagonistic and in cell lines it was synergistic (Figure 1b) In two cell lines (M249, M262) the combination was highly synergistic ... each single agent in BRAFV600E mutant cell lines, with an additional inhibitory effect against the wild type M257 cell line (Figure 2d) In two NRASQ61 mutant cell lines the combination induced ... representative cell lines is included in Additional File and a time course analysis in Additional File A summary of the key findings focusing on the 24 hour time point in BRAFV600E mutant cell lines is...

Ngày tải lên: 18/06/2014, 19:20

... produces infectious virions in BGMK cells FV3 produces infectious virions in BGMK cells BGMK cells were mock infected (A) or infected with FV3 at any MOI of (B) 48 hours post-infection cells were ... mammalian cell lines However, essential viral genes must express in mammalian cell lines since the virus is able to infect and successfully replicate in many cell lines, including rodent, human, ... FHM cells, an ectothermic cell line Although we demonstrated that FV3 can infect BGMK cells, we wanted to know whether FV3 produced infectious virions BGMK cells were either mock infected or infected...

Ngày tải lên: 20/06/2014, 01:20

... Medicine 2011, 9:64 http://www.translational-medicine.com/content/9/1/64 Page of 10 Figure SU11274 blocked motility in RMS cell lines A, SU11274 inhibited wound healing in RMS cell lines RMS cells ... chemiluminescence; EGFR: epidermal growth factor receptor; IGF-IR: insulin growth factor-I receptor; PI 3K: phosphoinositide-3-kinase; MAPK: mitogen activated protein kinase Acknowledgements We thank ... block c-Met function in RMS cell lines and normal muscle cell line, HASMC In CW9019 and RH30 cell lines, which expressed high levels of phospho-c-Met, the IC50 of SU11274 was 2.5 μM; whereas in...

Ngày tải lên: 20/06/2014, 03:20

... CGIs, histone modifications, and TF binding sites 1.0 Methylation 0.8 0.6 0.4 0.2 0.0 In Out CGIs In Out H 3K2 7ac In Out H 3K4 me3 In Out H 3K9 ac In Out TF binding sites Figure Distribution of methylation ... that fell within kb (red), within kb to 10 kb (purple), and within 10 kb to 50 kb (blue) of the original best-associated CpG-site The majority (96%) of probes within kb (red) were in the same CGI ... http://genomebiology.com/2011/12/1/R10 cells using phytohemagluttinin We obtained the transformed cell lines from the Coriell Cell Repositories Methylation data were obtained using the Illumina HumanMethylation27...

Ngày tải lên: 09/08/2014, 22:23

... http://www.jhoonline.org/content/1/1/14 Figure Arginase II expression in mRCC cell lines Arginase II expression in mRCC cell lines (A) After 48 hours in culture, CL-19 cells presented significantly more arginase ... of murine renal cell carcinoma cell lines and determine their effect on cell proliferation In adult mammals, the majority of endogenous L-arginine is synthesized from citrulline in the kidney ... L-arginine to synthesize NOHA from arginase, then increasing its inhibitory effect In contrast, it is also possible the growth of renal cell carcinoma cells is arginase II independent resulting in...

Ngày tải lên: 10/08/2014, 22:20

... acid residues involved in binding ATP The kinase insert domain contains a tyrosine residue that contributes to the binding of PI- 3K Asterisks indicate the position of aspartic acid814 in the second ... 1995) SCF-mediated c-Kit signaling in normal cells activates AKT, a survival-promoting serinethreonine protein kinase that enhances cell survival by inducing phosphorylation and inactivation of the ... mast cells release various mediators including histamine, leukotrienes, prostaglandins, serine proteases, and various cytokines, chemokines, and growth factors (Metcalfe, Baram et al 1997; Kinet...

Ngày tải lên: 24/08/2014, 11:29

... 3.3.3.4 Effect of knocking down of β-catenin on TA, hTERT mRNA and hTERT (949bp) in cancer cell lines 3.3.3.5 Effect of knocking down of β-catenin on telomere length in cancer cell lines 3.3.4 Effect ... luciferase activity in MCF7, HCT116 and 293T (n = 3; *P < 0.05, t-test) β-catenin knockdown caused telomere shortening in HCT116, MCF7 and MCF10A cells β-catenin knockdown in 293T cell lines does not ... regulation in cancer cell lines 3.3.5.2 β-catenin/TCF4 regulate hTERT promoter in cancer cell lines 3.3.5.3 Characterization of the distal TCF4 binding site (TBE) in the human hTERT promoter 3.3.5.4 In...

Ngày tải lên: 02/10/2015, 17:14

... substantially increased under 1% oxygen in KG1 and YN-1 cells, whereas HO-1 mRNA was undetectable in Jurkat and K5 62 cells (Fig 1A) In contrast, expression of HO-2 mRNA was detected in these four cell lines ... expression in MEL mouse erythroleukemia cells [32] Moreover, heme induces the expression of the a-globin gene in K5 62 human erythroleukemia cells [33] and the number of hemoglobin-producing cells in ... Experimental procedures Cell culture and hypoxia study The human cell lines used were KG1 myeloid cells, K5 62 erythroid cells, and Jurkat T-lymphocyte cells and were maintained in RPMI-1640 medium...

Ngày tải lên: 19/02/2014, 06:20

... thrombospondin carboxy-terminal cassette in cell extracellular matrix interactions and signaling Int J Biochem Cell Biol 36, 1102–1114 25 Dinser R, Zaucke F, Kreppel F, Hultenby K, Kochanek S, Paulsson ... expressed in HNK or RCC cells (Fig 3A,B); thus, there was no functional redundancy HNK cells expressed high levels of the calcium-binding protein, calbindin D2 8K [28] (Fig 3C) In normal kidney, calbindins ... from the indicated cell lines was subjected to semiquantitative RT-PCR with TSP1 primers HNK, normal cell strain; P769, PRC9, SW839, ARZ-1 and WT8, RCC cell lines (E) The same cell lines were...

Ngày tải lên: 07/03/2014, 05:20

... leukaemic cell lines Leukemia 16, 1705–1712 24 te Poele, R.H., Okorokov, A.L., Jardine, L., Cummings, J & Joel, S.P (2002) DNA damage is able to induce senescence in tumor cells in vitro and in ... increased in GEO but unchanged in HCT116 cells (B) Standard immunoblots for cyclin and CDK levels in GEO cells (C) Immunoprecipitation experiments highlighting cyclin/CDK interactions in the GEO cell ... drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy reduced CDK activity...

Ngày tải lên: 07/03/2014, 15:20

... antibodies against geminin showed that geminin protein levels are also increased in cancer cell lines, while different cancer cell lines appear to over-express geminin to varying degrees In order ... over-expression of the geminin protein differs between cell lines, resulting in gross differences in relative amounts of Cdt1 and its inhibitor in different cell lines It would be interesting to investigate ... primary cell lines Geminin is hardly detectable in the primary cell lines, and accumulates to higher levels in the majority of cancer cell lines tested It is noteworthy, however, that geminin levels...

Ngày tải lên: 07/03/2014, 16:20

... binding to nucleolin (black arrow) The competition experiments in UV cross-linking assays with the GT-recognizing proteins confirmed the results obtained with the total nuclear-enriched proteins: ... contrary, no cell growth inhibition effect was observed for GT-G4 The ability of these sequences to bind to proteins forming the CRC of 45 ± kDa was checked by UV cross-linking assays in competition ... T % of cellular growth A Competitor -Proteins + + + + 116 kDa 70 kDa 46 kDa 32 kDa 23 kDa Fig Cytotoxic assay of GRO26A and GRO29A and their binding to nuclear proteins (A) CCRF-CEM cells...

Ngày tải lên: 16/03/2014, 13:20