isolation reverse transcriptase rt pcr and real time pcr

... is further supported by the robust constitutive expression by microglia and astrocytes of IPS-1 [23], STING, and RIP3, reportedly critical downstream effector molecules for DAI [29-31] Importantly, ... cultures and carried out the in vitro and in vivo experiments, performed data analysis, and drafted the manuscript VSC participated in the performance of the in vivo viral infections and the isolation ... (Panels A and C) or astrocytes (Panels B and C) were untreated (0) or infected with HSV-1 (MOI of 0.1, and 10) or MHV-68 (MOI of and 10) At 24 hours post-infection expression of DAI (Panels A and B),...

Ngày tải lên: 19/06/2014, 22:20

... MHz, with tetramethylsilane as the internal standard Chemical shifts (δ) are reported in parts per million (ppm) and the coupling constants (J) in Hertz (Hz) Fourier transform infrared absorption ... with diluted hydrochloric acid (1:3), and the precipitate was filtered and recrystallized from DMF and water A mixture of the acids 3a–k and 4a–c (1 mmol), and phosphorus oxychloride (5 mL) was ... was inverted over crushed ice In some cases the excess of phosphorus oxychloride was removed under reduced pressure before inverting over crushed ice and neutralized The new compounds 1a–k and 2a–c...

Ngày tải lên: 20/06/2014, 20:20

Ngày tải lên: 19/04/2015, 05:00

... AB024737.1 and TECL5B_TACTR; AB024738.1) (iii) Angiopoietin: angiopoietin and precursors from mouse (AGP1_MOUSE; O08538 and AGP2_MOUSE; O35608), bovine (AGP1_BOVIN; O18920 and AGP2_BOVIN; O77802), and ... Biochem 271) 1927 RNA preparation and Northern blot analysis RNA was extracted from liquid-nitrogen pulverized tissue using TRIzol reagent (GibcoBRL, Grand Island, NY, USA), as described previously ... was used for visualization of the signals Antisense and sense single stranded DNA digoxigenin-labeled probes were synthesized by PCR using the PCR DIG Probe synthesis Kit (Roche) Sense probes were...

Ngày tải lên: 16/03/2014, 16:20

... Klippert and S Neander for expert technical assistance We would also like to thank F Martins-Wess for help with the RTPCR experiments REFERENCES International Human Genome Sequencing Consortium ... Northern analyses and in the RT- PCRs, the two experiments clearly indicate a tissue-specific expression pattern of the NICN1 gene Tissues with strong NICN1 expression are brain, testis, liver and ... conditions Fig Semiquantitative RT- PCR analysis of human and murine NICN1 genes Normalized cDNAs from different tissues were used as templates in semiquantitative RT- PCR experiments In the upper...

Ngày tải lên: 23/03/2014, 21:20

... terminal hair in various parts of her body and four angiofibromas in her face, were observed Her arterial blood pressure was moderately abnormal (155/80 mmHg), while she reported menstrual irregularity ... ultrasound and Tc99 m Sestamibi-scans both disclosing hyperplasia of the parathyroid glands, abdominal MRI scan and endoscopic ultrasound (EUS) both disclosing a 4.5 × 3.0 cm tumor in the body and tail ... caryokinesis and invasion of venous and sinusoid vessels were also observed Necrotic areas were present only in the tumor of the left adrenal gland Immunohistochemically, the cells were MelanA and synaptophysin...

Ngày tải lên: 09/08/2014, 01:24

... shRNA MAEC were treated with LPS and IFN for 0–8 h B, Cells were treated with medium or LPS and IFN for 24 h mRNAs encoding COX-2 and β-actin were determined by RT- PCR and agarose gel electrophoresis ... LPS and IFN for 24 h Cycloheximide (90 μg/ml) was added, and cell extracts were collected at the indicated times and western blotted for COX-2 and α-tubulin A, Representative images of COX-2 and ... primary and secondary stimuli that may be present, and by cellular factors, such as supply of arachidonic acid, complement of various prostaglandin synthases, and expression of prostaglandin receptors...

Ngày tải lên: 11/08/2014, 08:22

... the study, and participated in its design and coordination, QL and LL revised the manuscript LW and LL participated in the design of the study and performed analysis, LW, CD and YZ participated ... cell transfected and infected were harvested and extracted for further real- time PCR The protocol is described in Material and Method a Viral loads shown with the copy of α-4, tk and gC genes of ... 24, 27, 36, 46, 60 and 72 h post-infection, samples of cell infected by HSV1-UG were collected and measured by real- time PCR with specific primers against α-4 gene n = for all time points Error...

Ngày tải lên: 12/08/2014, 04:20

... = 107 bp) was used Quantitative real- time PCR was performed as we described previously [33] Real- time PCR was performed in triplicate for each sample from each time point Relative gene expression ... and then harvested cells at 0, 4, 12, 24, and 48 hr postinfection Total RNA was isolated as described in Materials and Methods, and various viral mRNA levels were quantitated by RT- PCR ICP0 and ... Finally, 1000 ng of total RNA was reverse- transcribed using random hexamer primers and Murine Leukemia Virus (MuLV) Reverse Transcriptase from the High Capacity cDNA Reverse Transcription Kit (Applied...

Ngày tải lên: 12/08/2014, 04:21

... = 107 bp) was used Quantitative real- time PCR was performed as we described previously [33] Real- time PCR was performed in triplicate for each sample from each time point Relative gene expression ... and then harvested cells at 0, 4, 12, 24, and 48 hr postinfection Total RNA was isolated as described in Materials and Methods, and various viral mRNA levels were quantitated by RT- PCR ICP0 and ... Finally, 1000 ng of total RNA was reverse- transcribed using random hexamer primers and Murine Leukemia Virus (MuLV) Reverse Transcriptase from the High Capacity cDNA Reverse Transcription Kit (Applied...

Ngày tải lên: 12/08/2014, 04:21

... trng cho phần tử tập hợp: VD : - tập hợp 100 số nguyên dơng đầu * GV lấy VD, rõ tính chất đặc trtiên đợc viết là: ng.Yêu cầu HS làm H2 SGK, A = { n Z \1 n 100} gọi HS trả lời, học sinh câu:...

Ngày tải lên: 26/06/2013, 01:25

Ngày tải lên: 30/08/2013, 13:58

Ngày tải lên: 05/09/2013, 10:46

Ngày tải lên: 12/12/2013, 16:15

Ngày tải lên: 24/12/2013, 16:15

... way to our fifty thousand members as an educational and propaganda machine Music, obviously, can make a mood, build familiarity and memory, and for an happy event He has always been to help the ... people crowded in and the reality began to fully sink in, I felt I couldn't breathe I stepped out on the porch, and the sight beyond the gates was incredible; tens of thousands of people gathered ... important — you should wear something which your own self esteem and creates the right kind of impression I have always the kindness of my friends We have to find a way to our fifty thousand...

Ngày tải lên: 25/01/2014, 22:20

... pitch standard (g = 2.0028) A solution of 10 mm CuSO4 in m NaClO4 and 10 mm HCl was used as the standard for spin integration Further EPR conditions are provided in the legend to Fig Q-band EPR ... sequencing and A Burkowski for the plasmid pXMJ19 G Auling and P Stolle appreciate the help of J Stubbe and J Cotruvo with respect to improving the manuscript The work was supported in part by the ... analysis and multifrequency EPR provides additional support for this assignment Materials and methods Chemicals 2¢,5¢-ADP Sepharose (self packed XK 16 ⁄ 20), UNOTM sphere Q (self packed XK 16 ⁄ 20) and...

Ngày tải lên: 15/02/2014, 01:20

Ngày tải lên: 19/02/2014, 06:20

... RT- PCR experiments, whereas the histogram shown in the lower part of the figure represents data from real- time quantitative RT- PCR analysis Semiquantitative RT- PCR and real- time quantitative RT- PCR ... were scanned and quantified by densitometry by using QUANTITY ONE software (Bio-Rad, Hercules, CA, USA) Real- time quantitative RT- PCR Quantitative RT- PCR was performed by using the qPCR core kit ... treated with EPA and DHA, the Egr-1-binding activity seems to be less intense than in AAenriched cells In order to verify these results, we used semiquantitative and real- time RT- PCR analysis to...

Ngày tải lên: 19/02/2014, 16:20