investigation in animal and in vitro studies

... encoding regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell-based, in vitro and in silico ... providing the recombinant IKK2 (rhIKK2) and Nandini Kishore for providing the SC-514 inhibitor We thank also Paul Hayter, Sasha Sreckovic and Matthew Strawbridge with help in growing and analyzing ... In vitro analysis of NF-jB signaling pathway 46 Peet GW & Li J (1999) IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin...

Ngày tải lên: 16/03/2014, 11:20

... Furthermore, increased radiosensitivity in the androgen independent PC cell line PC-3 has been demonstrated in vitro and in vivo [26,27] Our study analyzes the interactions of irradiation and genistein ... several intracellular and extracellular pathways including cell cycle control, apoptosis, and angiogenesis [12-14] In PC cell lines genistein incubation proved to have many effects in vitro Among ... that genistein acts as an inhibitor of steroidogenesis, blocks several protein tyrosine- and histidine kinases [810], and inhibits topoisomerase I and II [11] These effects result in alterations...

Ngày tải lên: 09/08/2014, 09:22

... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... inflammation following SIT therapy In conclusion, our in vivo and in vitro studies presented the evidence that not only Th2 but also Th17 mediated inflammation was involved in the AR pathological...

Ngày tải lên: 23/09/2015, 14:47

... extrinsic or intrinsic discoloration46, 85-87 Extrinsic discoloration may result from surface staining, marginal staining due to microleakage and changes in surface morphology by wear The intrinsic ... machining dental porcelain (CEREC) Cerestore injection-molded core Combined etched enamel/porcelain technique to resin bonded restoration Re-introduced the method of etching porcelain, for resin-bonded ... Sew Meng and Dr.Girija for their sincere support and guidance during the period of my study i I take immense pleasure in thanking my parents, for constantly encouraging and supporting me in all...

Ngày tải lên: 09/10/2015, 11:24

... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...

Ngày tải lên: 07/03/2014, 21:20

... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... and O-chain polysaccharide of A salmonicida strain 80204-1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and...

Ngày tải lên: 23/03/2014, 13:20

... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... previously in the bovine model Increases in Akt and Erk signalling proteins in response to FSH and IGF stimulation suggest a role for Akt and Erk signal transduction pathways in FSH and IGF mediated ... for luteinizing hormone and its interaction with insulin: activation of Janus kinase/signal transducer and activator of transcription and phosphoinositol 3-kinase/Akt pathways Endocrinology 2003,...

Ngày tải lên: 20/06/2014, 07:20

... solution containing the rutin (120 ml) was added to this organic phase maintaining the agitation for a further 10 Then, the organic phase was injected into an aqueous phase (534 ml) containing Tween ... rutin-loaded nanocapsules and nanoemulsions, as aqueous intermediate or final systems to the development of nanomedicines containing rutin Both formulations presented an increase in the rutin ... Biosystems) containing ml of the sample (0.25 mg ml-1) was put into a glass test-tube containing 200 ml of dissolution medium This system was maintained under constant moderate stirring during all the...

Ngày tải lên: 21/06/2014, 17:20

... immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated from feces of healthy infants ... Serum and Vaccine Producer Biomed, Cracow, Poland), Salmonella enterica subsp enterica sv Enteritidis (clinical isolate obtained from the Institute of Sea and Tropical Medicine, Gdynia, Poland), ... KALLIOMAKI M., KIRJAVAINEN P., EEROLA E., KERO P., SALMINEN S., ISOLAURI E.: Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing, J.Allergy Clin.Immunol 107,...

Ngày tải lên: 07/07/2014, 19:20

... defined confidence interval for the scaling exponent Note that no information on metabolic rates in the studies of Heusner and Kleiber is used in generating the BMR predictions Discussion In ... positive and strictly decreasing as y and M increase, the above derivative is strictly increasing The second prediction is that the above slope approaches BMRv = avM0.86, βn as M increases (and y increases) ... respectively, and 26 µm and 28 µm in horses and cows, respectively [38], and the scaling exponent for thickness of this layer is approximately 0.09 Combining this exponent with an estimate of the scaling...

Ngày tải lên: 13/08/2014, 23:20

... reduction in glucose and insulin levels 15mins after oral glucose load in an OGTT [111] However, no effect was observed on fasting glucose and insulin levels, contradicting the previous finding in normaglycemic ... caffeine, and (iv) CGA possesses hypoglycemic effects in human, animal and in vitro models, the present author's next aim is to investigate the hypoglycemic effects of CGA in both in vivo and in vitro ... Activation of insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis in liver and kidney [14], translocation of glucose transporter-4 (GLUT 4) from inner...

Ngày tải lên: 08/09/2015, 18:22

... factor in determining the biocompatibility of the dendritic devices (Wolinsky and Grinstaff, 2008) Large and positively-charged amine-coated melamine dendrimers, for instance, are found to induce in ... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... contains platinum in the molecular structure The cytotoxic effect of cisplatin is mainly contributed from the platinum complexes which can bind and interact with the basic sites of DNA, resulting...

Ngày tải lên: 09/10/2015, 11:18

... foetal bovine serum g gram h hours xv H&E haematoxylin and eosin stain HRP horseradish peroxidase ICE interleukin-1β-converting enzyme IFN interferon IL interleukin INF infected IRF interferon-regulatory ... anchored in the envelope, functions as an ion channel between the interior of virus and its environment and maintains a low pH during virion uncoating during initial stages of infection (Pinto, Holsinger ... nucleotide-binding domain-leucinerich repeat-containing molecules (NLRs) TLRs are transmembrane proteins with an extramembranous domain comprising leucine-rich repeats involved in ligand recognition...

Ngày tải lên: 13/10/2015, 16:41

... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... (GIBCO, USA) containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All ... then evaluated by in vitro testing in human cancer cell lines We observed that 198 RGD-M-LCL had increased anti-proliferative activity and increased the induction of apoptosis in cancer cells compared...

Ngày tải lên: 26/10/2012, 08:57

... domain mapping of proteins where fragments that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... nonbiotinylated single strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can be stored and reused for further reactions into solution and leaving...

Ngày tải lên: 25/10/2013, 22:20

... English and Vietnamese idioms denoting work in term of the contrastive analysis and to classify them in accordance with their semantic and syntactic feature 3) Finding and making a comparison and ... or examples used in helps to describe and demonstrate linguistics features of idioms contexts, in short stories, novels, newspapers and magazine in both denoting work in English and Vietnamese ... of learning and teaching English idioms denoting work FINDINGS AND DISCUSSION 4.1 SYNTACTIC FEATURES OF ENGLISH AND VIETNAMESE IDIOMS DENOTING WORK 4.1.1 Syntactical Glimpse at English and Vietnamese...

Ngày tải lên: 26/11/2013, 13:16

... EF-2 in the mitochondrial apoptotic response induced by the toxin remains to be determined Finally, we assign an important role to the endosomal acidic cathepsins B and D in increasing the in vitro ... death (5 postinjection), suggesting a high binding capacity of ETA in hepatic parenchyma Indeed, injection of the toxin into mice has been shown to result in an early and profound inhibition of ... endosomes containing in vivo internalized ETA The endosomal fraction was isolated 30 after the administration of ETA, and then resuspended in 0.15 M KCl containing mM MgCl2 and, when indicated, 50...

Ngày tải lên: 18/02/2014, 04:20

... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids...

Ngày tải lên: 18/02/2014, 08:20

... researched For instance, problems with raising and using finance and with obtaining and applying information on costs and control, as much due to external constraints as lack of financial management ... theory (increased earnings coming from additional investment in training and education) not hold in the secondary sector (King, 1990), a series of awareness and training programmes continue to ... in their communities and so on Finally, we can come to some understanding of the role that interventions such as training and education, financial incentives and public recognition may play in...

Ngày tải lên: 19/02/2014, 21:20

... template and binding properties of mutated and deleted RNA fragments of the 3¢-end of the minus-strand HCV RNA in further detail This study should lead to interesting interpretations since it ... domain in the 3¢-end of the HCV minus-strand RNA inhibits in vitro initiation of RNA synthesis by the viral polymerase [19] Kashiwagi et al [20] studied RNA synthesis by recombinant HCV NS5B using ... unavailable In the present study we investigated the involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using new mutants of the 3¢-end of minus-strand RNA...

Ngày tải lên: 20/02/2014, 01:20