interaction of surfactants with environmental pollutants

... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin DISCUSSION The binding of decorin with fibrillar collagens has been extensively ... (number of residues) and melting temperature of the trimeric species of CNBr peptides The bottom two lines indicate the N fi C direction with a length scale (in residues) and the banding pattern of...

Ngày tải lên: 21/02/2014, 15:20

... with the cDNA probes to isolate the full-length cDNAs of annexins Expression of annexins in E coli The coding region of each annexin was amplified by PCR with a pair of primers The sequences of ... mass of 38 kDa with pI values of 6.2–7.1 (annexin A1), and of c (annexin A2), and a single spot at 35 kDa with pI ¼ 5.6 (annexin A5) Each annexin subtype is indicated by a circle Colocalization of ... Immunofluorescence double-staining of one subtype of annexin with the other subtype of annexin A section was treated with rabbit antiserum raised against one subtype of annexin (red: A, annexin A1;...

Ngày tải lên: 07/03/2014, 05:20

... structure of DAPI-bound ATTtemplate with respect to its free form, which is consistent with the previously reported lower mobility of the free monomeric random coil structure of (ATT)6 in comparison with ... property of DAPI could only be attributed to a direct interaction of the drug with the Klenow fragment A DNA-mediated step correlating with the different structural and binding characteristics of DAPI–template ... melting studies are also consistent with a double-helix structure of ATT-template complexed with the drug Binding of DAPI strongly increases the melting temperature of the ATT-template from 17 °C...

Ngày tải lên: 16/03/2014, 23:20

... indicating that the interactions of these different proteins are likely to be indirect, depending on the presence of intact RNA as integral component of the RNP Association of 42Sp50 with mRNA has ... family of RNA binding proteins [31–33] Together with the findings obtained in the present study, interaction of VICKZ and poly(A) binding proteins thus appears to define a conserved feature of mRNPs ... overnight at °C If indicated, lL of RNase A (10 mgÆmL)1) was added together with the antibody Immunopellets were washed with ice-cold IPP145, mixed with 30 lL of · SDS loading dye and analyzed...

Ngày tải lên: 23/03/2014, 03:20

... Journal compilation ª 2009 FEBS H Hemmi et al Interaction of EW29Ch with sugars Identification of sugar-binding sites The interaction of 15N-labeled EW29Ch with lactose, melibiose, galactose, methyl ... et al Interaction of EW29Ch with sugars A B Fig Close-up of the 1H–15N HSQC regions showing the chemical exchange for Asp18 or Trp33sc with increasing amounts of some sugars Peak movements of main ... structure of EW29Ch with lactose showed that the glucose residue of the lactose molecule interacted with subdomain c of EW29Ch [25] This interaction may be an artifact caused by the crystallization of...

Ngày tải lên: 23/03/2014, 04:21

... either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide dismutase ... minutes of anaerobiosis, small amounts of sodium dithionite solution were added to attain a level of reduction of heme b and hemin of 90–95% Sequential additions of small aliquots (0.2 lL) of a ... presence of a detergent or of a mutation such as Arg54Ser [14], the structural arrangement of the FAD binding domain of flavocytochrome b is modified, resulting in a loss of interaction of Babior,...

Ngày tải lên: 31/03/2014, 21:21

... [17] of patients with RA, but that has not been studied extensively in RA due to controversial data on its role in the pathogenesis of disease [18] The present research on the interaction of RA-IgG ... in vivo model of cartilage destruction Although a number of molecular pathways have been identified that contribute to the stable activation of RASFs, the precise cause and nature of this activation, ... colleagues that in a cohort of patients with recent onset arthritis, patients who later developed RA showed significantly higher serum levels of IL-16 than patients with undifferentiated arthritis...

Ngày tải lên: 09/08/2014, 06:22

... parts of the metabolism However, it is often interesting to get a quick and complete overview of the whole data set, especially at the start of the analysis Furthermore, many tools are only offered ... infection, a lag phase of several years is often observed before the appearance of symptoms whose intensity on a given plant may vary with each year However, infected plants die within a few years ... metabolic profiling of grape (Vitis vinifera L.) leaves [49] Here we present an overview of the transcriptional changes in a susceptible grapevine – Eutypa lata interaction at the time of symptoms...

Ngày tải lên: 12/08/2014, 03:21

... replacement of G with A leads to a loss of one hydrogen bond along with appositional interactions in the donor atoms of the protein Replacement with C, on the other hand, results in the loss of both ... principal vector of the interfacial residues in the cognate (PV1,cog) and noncognate (PV1,noncog) complexes within 0.35 nm of the point of substitution (Region R4) and within 0.35 nm of the full recognition ... case of protein-DNA interactions Even though there is a plethora of studies of protein dynamics when they are present alone [102-113], studies of protein dynamics available in the context of protein-DNA...

Ngày tải lên: 09/09/2015, 18:49

... Co-localization and interaction of ZO-2 and ZO-3 with Scrib in COS-1 Figure 2-3 Scrib interacts directly with ZO-2 and ZO-3 via its PDZ domains Figure 2-4 Co-localization of ZO-2 and ZO-3 with Scrib in ... to the direction of movement, with the anterior facing the migration front and set off a series of processes that produces cell motility Foremost of these is the generation of actin mediated ... contribution of reagents but also his support during the course of my project and advice throughout the writing and revision of the publication of my work My candidature in the Institute of Molecular...

Ngày tải lên: 14/09/2015, 08:24

... Figure 8-4 Cumulative wear (µm) of KM in different acidic conditions 152 Figure 9-1 Shear strength of FN and KM 154 Figure 9-2 Illustration of interaction of GIC with environmental phosphate and ... prevention of caries of adjacent tooth (Forsten, 1991) 15 Chapter 2.1 Development of GICs One of the main limitations of GICs is the lack of physico-mechanical properties This limits the use of GICs ... Professor Gao Xuejun, current Head of Department of Cariology, Endodontology and Operative Dentistry, School and Hospital of Stomatology, Peking University, Professor Wang Jiade, former Head of...

Ngày tải lên: 15/09/2015, 17:11

... the active sites of this enzyme and arginase I In spite of the relatively low agmatinase activity of the Asn149Asp variant, it is clear that the interactions of arginase II with l-arginine and ... blue staining of purified enzymes Metal-free species of purified enzymes were obtained by incubation for h at 25 °C with 25 mm EDTA and m 4545 ´ V Lopez et al Interaction of arginase II with substrate ... invariant Km value upon mutation of these residues agree with the currently accepted mechanism for the arginase reaction, which considers the 4541 Interaction of arginase II with substrate and manganese...

Ngày tải lên: 20/02/2014, 02:21

... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the light ... intensity of the band of unhydrolyzed actin was plotted against the time of incubation Characterization of actin preparation quality Fluorescence parameters were used for estimation of nativity of actin ... parameter A of actin was close to 2.4 Increase of the time of heating at 43 °C leading to decrease of parameter A up to 2.2–2.3 was accompanied by further decrease of the initial rate of polymerization...

Ngày tải lên: 20/02/2014, 23:20

... equilibrated with 10 column volumes (10 · 0.2 mL) of buffer B, with subsequent washing four times with five volumes of the same buffer Proteins were eluted with buffer E (buffer B supplemented with 250 ... for nonspecific interactions Figure 2A shows a response difference sensorgram (FC2 – FC1) of interactions of GlnK with immobilized TnrA For this analysis, an analyte concentration of 40 nm GlnK ... and incubated with 0.5 mL of antiserum for h at 24 °C with gentle shaking After being washed times with mL of 0.2 m Na3BO3 (pH 9.0), the Sepharose beads were resuspended in mL of 0.2 m Na3BO3...

Ngày tải lên: 06/03/2014, 00:21

... analysis The interaction of soluble b chains with SAgs was monitored in a resonance mirror with an IAsys instrument (Labsystem, Cambridge, UK) biosensor, which allows determination of real time interactions ... lower Kd The Kd calculation is independent of the amount of Ó FEBS 2004 Interaction of human TCR with superantigen SSA (Eur J Biochem 271) 4081 Fig Vb1–SSA interaction analysis Association curves ... the binding of Vb1–SSA with Kd ¼ 10.82 lM (E) and Vb1–SSA(C26S) with a Kd ¼ 9.14 lM (F) Fig SSA dimer in supernatant of S pyogenes Supernatants of isolates from patients infected with S pyogenes...

Ngày tải lên: 07/03/2014, 16:20

... contribution of electrostatic interactions to the binding of both peptides with negatively charged SUVs, titration of the peptides with PtdCho ⁄ PtdSer vesicles was carried out in the absence of salt ... report on the interaction of an N-terminal (E2(7–26)) and an internal (E2(279–298)) synthetic peptide sequence of the E2 structural protein of HGV ⁄ GBV-C with phospholipid membranes of different ... presence of PamOlePtdCho ⁄ PamOlePtdGro SUVs The electrostatic interactions were further studied by titration of the peptides with egg PtdCho ⁄ brain PtdSer (65 ⁄ 35) SUVs in Tris ⁄ HCl buffer without...

Ngày tải lên: 07/03/2014, 17:20

... study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers Interaction of stefin B in the prefibrillar aggregated state with model lipid membranes ... channel hypothesis of AD and neurodegeneration in general, is not incompatible with other key elements of toxicity, as, for example, the deregulation of Ca2+ homeostasis and generation of reactive oxygen ... stefin B which is more basic, with an isoelectric point of ~ 8, than stefin A, with an isoelectric pont of ~ An additional factor may be the much higher stability of stefin A which also may count...

Ngày tải lên: 07/03/2014, 17:20

... and ABP96, but no interaction with ABP64 (Table 1) Ó FEBS 2002 Calliphora anterior fat body protein (Eur J Biochem 269) 957 Table Interaction of AFP with different fragments of the hexamerin receptor ... predicted protein is composed of 306 amino acids, with a calculated molecular mass of 34.3 kDa and a pI of 5.72 Similar searches with the deduced amino-acid sequence of full-length Calliphora AFP, ... results of the immuno-coprecipitation revealed that AFP interacts with P30 and with all cleavage products of the hexamerin Expression of AFP in C vicina As in almost all experiments dealing with...

Ngày tải lên: 08/03/2014, 22:20

... AB koff;1 kon;2 AB þ B )* AB2 koff;2 ð2Þ ð3Þ where d½BŠ=dt ¼ À 2kon;1 ½AŠ½BŠ þ koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2 ½AB2 Š ð4Þ d½ABŠ=dt ¼ 2kon;1 ½AŠ½BŠ À koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2 ... vicinity of the membrane We found that the isolated factor X Gla domain exhibits low affinity binding to PtdSer-containing membranes with a Kd of 4.6 lM This agrees well with the value of 2.4 lM ... that mutation of Ca2+ ligating amino acids in the N-terminal part of the first EGF-like domain of factor X influences the amidolytic activity of the intact protein [36] However, direct interactions...

Ngày tải lên: 08/03/2014, 23:20

... behavior of trehalase depending on the nature of the activation stress (Fig 5B) This shift might argue against the participation of a unique set of interactions involving association of Ntp1p ... the regulation of Ntp1p activity under stress relies on the existence of some kind of interaction between Ntp1p and Tps1p, or other elements, that may be lost under the conditions of the in vitro ... identification of strains C3 and C69, with one copy of Ntp1p–Ha6H expressed from the genomic ntp1+ promoter, was verified by Southern blot analysis and immunoblot of whole-cell extracts with antiHa...

Ngày tải lên: 08/03/2014, 23:20