... behavior of trehalasedepending on the nature of the activation stress (Fig. 5B).This shift might argue against the participation of a uniqueset of interactions involving association of Ntp1p ... identification of strains C3 andC69, with one copy of Ntp1p–Ha6H expressed from thegenomic ntp1+promoter, was verified by Southern blotanalysis and immunoblot of whole-cell extracts with anti-Ha ... the regulation of Ntp1p activity under stress relieson the existence of some kind ofinteraction between Ntp1pand Tps1p, or other elements, that may be lost under theconditions of the in vitro...
... correlate with the binding affinity between GA and the receptor.The thermodynamic parameters obtained are in therange of those of other interactions with c-CD [15].The interactionof GA with c-CD ... observed with the interaction of GA and c-CD [21,22]. In addition, theDCpvalue, )0.14 kcalặmol)1ặC)1, is large and negat-ive, which is in the largest range among those of otherinteractions with ... of molecule number was shown against the logarithm of molecule diameter. Interaction of gymnemic acid with cyclodextrins Y. Izutani et al.6158 FEBS Journal 272 (2005) 61546160 ê 2005 FEBS Interaction...
... value of the amplitude of the states of OFIẳ 0, with respect to the initial state jCIi at t ẳ 0, increases with increasing t, while the amplitudes of the states of OFI6ẳ 0 oscillate withoutsubstantial ... VdNdEẳVhh12cp3n2rngị!2dO 2:59ị Interaction ofElectrons and Photons 31Copyright â 2004 Marcel Dekker, Inc. 2 Interaction ofElectrons and PhotonsThis chapter provides the ... existence of eigenstates of infinitively large negative n. Since such eigenstates are not Interaction ofElectrons and Photons 21Copyright â 2004 Marcel Dekker, Inc. The Hamiltonian for the energy of...
... which of the physical and chemical characteristics of particulate air pollution are responsible for health effects? 8. What is the evidence of synergy / interactionof the pollutant with other ... California, prevalence of asthma was not associated with ozone levels among the 12 studied communities (231). On the contrary, prevalence of asthma increased with average levels of O3 among the ... association. However, difficulties in diagnosis of asthma using self-reporting of symptoms and limitations of prevalence studies with no control of in/out migration could explain these differences....
... into the interactionof human arginase II with substrate and manganese ions by site-directedmutagenesis and kinetic studiesAlteration of substrate specificity by replacement of Asn149 with AspVasthi ... spite of the rel-atively low agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altered by replace-ment of ... bluestaining of purified enzymes.Metal-free species of purified enzymes were obtained byincubation for 1 h at 25 °C with 25 mm EDTA and 3 mV. Lo´pez et al. Interactionof arginase II with substrate...
... intensity of the band of unhydrolyzed actin was plotted against the time of incubation.Characterization of actin preparation qualityFluorescence parameters were used for estimation of nativity of ... aggregation of partiallyfolded actin.To obtain more detailed information on the interaction of HSP25 with partially unfolded actin, we analyzed theeffect of different quantities of HSP25 on ... parameter A of actin was close to2.4. Increase of the time of heating at 43 °C leading todecrease of parameter A up to 2.2–2.3 was accompanied byfurther decrease of the initial rate of polymerization....
... probableregions of interaction, in agreement with the contacts with GrpE and the results obtained from experiments with mutants. The contact r egions predicted w ith our methodand t he implicit model ofinteraction ... of the protein structure is the detection of regions ofinteraction by the presence of specific familysignatures in the m ultiple s equence alignment a ble t odiscriminate different t ypes of ... retained, independent of theirclassification. Furthermore, a 40% sequence identity cut-offfor protein homology is used instead of the present 30% andthe definition of the interaction surface is...
... spectra of a recombinant decorin purified in theabsence of chaotropic agents, with the exception of thewavelength of the minimum (215–216 instead of 218 nm)and very different to the spectrum of ... 2002 Interactionof decorin with collagen peptides (Eur. J. Biochem. 269) 1431 with respect to the unmodified ones is not related to thepercentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin.DISCUSSIONThe binding of decorin with fibrillar collagens has beenextensively...
... basallevels of resonance units (GlnK free surface) withinseconds. To test the effects of various molecules on the interaction of TnrA with GlnK, 40 nm GlnK (trimer)Fig. 1. Coimmunoprecipitation of TnrA, ... equilibrated with 10 column volumes(10 Ã 0.2 mL) of buffer B, with subsequent washing fourtimes with five volumes of the same buffer. Proteins wereeluted with buffer E (buffer B supplemented with 250 ... (SPR) of theGlnK–TnrA interaction As a next step, the interactionof TnrA with GlnK wasinvestigated in vitro by BIAcore SPR detection. Forthis analysis, a Strep-tag II-tagged variant of GlnK(GlnK-ST)...
... forthe interactionof frog annexin A1 and annexin A2 with dicalcin. Consistently, we observed that the35 kDa forms of annexin A1 and annexin A2 foundin the fraction of the binding proteins of dicalcin(Fig. ... presence of dicalcin and all of the annexins (E) was compared with the calcu-lated sum of each of the initial rates obtained in (B)–(D) (thick dot-ted lines).T. Uebi et al. Role of dicalcin ... Comprehensive interactionof dicalcin with annexinsin frog olfactory and respiratory ciliaTatsuya Uebi1, Naofumi Miwa1,2,* and Satoru Kawamura1,21 Department of Biology, Graduate School of Science,...