interaction of electron with matter pdf
Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf
... forthe interaction of frog annexin A1 and annexin A2 with dicalcin. Consistently, we observed that the35 kDa forms of annexin A1 and annexin A2 foundin the fraction of the binding proteins of dicalcin(Fig. ... presence of dicalcin and all of the annexins (E) was compared with the calcu-lated sum of each of the initial rates obtained in (B)–(D) (thick dot-ted lines).T. Uebi et al. Role of dicalcin ... 4869Comprehensive interaction of dicalcin with annexinsin frog olfactory and respiratory ciliaTatsuya Uebi1, Naofumi Miwa1,2,* and Satoru Kawamura1,21 Department of Biology, Graduate School of Science,...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
... spectra of a recombinant decorin purified in theabsence of chaotropic agents, with the exception of thewavelength of the minimum (215–216 instead of 218 nm)and very different to the spectrum of ... 2002 Interaction of decorin with collagen peptides (Eur. J. Biochem. 269) 1431 with respect to the unmodified ones is not related to thepercentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin.DISCUSSIONThe binding of decorin with fibrillar collagens has beenextensively...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... the interaction of TATAbox binding protein and TFIIA with DNA. Biochemistry 33,7033–7040.4760 E. Trotta et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Interaction of DAPI with individual strands of ... compact structure of DAPI-bound ATT-template with respect to its free form, which is consistent with the previously reported lower mobility of the freemonomeric random coil structure of (ATT)6in ... primer-complementary region of the template. (C) Chemical structure of DAPI.4756 E. Trotta et al. (Eur. J. Biochem. 270) Ó FEBS 2003the interaction with DAPI. This suggests an efficientmechanism of single-stranded...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... indicating that the interactions of these differ-ent proteins are likely to be indirect, depending on thepresence of intact RNA as integral component of theRNP.Association of 42Sp50 with mRNA has ... that areformed with the corresponding signal sequences in the untranslated regions of the mRNAs and with a number of different so-called localization proteins.In the context of the present study, ... over-night at 4 °C. If indicated, 5 lL of RNase A (10 mgÆmL)1)was added together with the antibody. Immunopellets werewashed with ice-cold IPP145, mixed with 30 lLof2· SDSloading dye and analyzed...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... barelyinteracts with the region of H1 and the O-methylgroup. The crystal structure of EW29Ch with lactoseshowed that the O2, O3 and O4 atoms of the galac-tose residue of lactose formed hydrogen bonds with EW29Ch, ... tighter interaction with lac-tose than the c sugar-binding site because of the num-ber of intermolecular hydrogen bonds and of residuesinteracting with lactose. Our results agreed well with those ... structure of EW29Ch with lactose showed that the glucose residue of the lactosemolecule interacted with subdomain c of EW29Ch[25]. This interaction may be an artifact caused by thecrystallization of...
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Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf
... )*kon;1koff;1AB ð2ÞAB þ B )*kon;2koff;2AB2ð3Þwhered½B=dt ¼À2kon;1½A½Bþkoff;1½ABÀkon;2½AB[B]þ 2koff;2½AB2ð4Þd½AB=dt ¼ 2kon;1½A½BÀkoff;1½ABÀ kon;2½AB[B] þ 2koff;2½AB2ð5Þd½AB2=dt ... that mutation of Ca2+ligatingamino acids in the N-terminal part of the first EGF-likedomain of factor X influences the amidolytic activity of theintact protein [36]. However, direct interactions ... vicinity of the membrane.We found that the isolated factor X Gla domain exhibitslow affinity binding to PtdSer-containing membranes with aKd of 4.6 lM. This agrees well with the value of 2.4...
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Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf
... behavior of trehalasedepending on the nature of the activation stress (Fig. 5B).This shift might argue against the participation of a uniqueset of interactions involving association of Ntp1p ... identification of strains C3 andC69, with one copy of Ntp1p–Ha6H expressed from thegenomic ntp1+promoter, was verified by Southern blotanalysis and immunoblot of whole-cell extracts with anti-Ha ... the regulation of Ntp1p activity under stress relieson the existence of some kind of interaction between Ntp1pand Tps1p, or other elements, that may be lost under theconditions of the in vitro...
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Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf
... with the 3¢ of the )148 to )124 region of c-jun. However, this interaction was not found to be absolutely essential f or its interaction with RLjunRP c omplexed with Jun-25. The interaction of rRLjunRP ... groove of the )148 to )124 region of c-junTo establish the nature of interaction b etween trans-actingfactor(s) and t he )148 to )124 r egion of c-jun,drugsthatspecifically interact with the ... site of offactors int eracting with the )148 to )124 region of c-jun. (A,B) Effect of distamycin A (Dist. A)on complex f ormation. Standard EMSAreactions were carried o ut us ing 1 ng of labelled...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
... lLof6.5pMT4 DNA weremixedwith1lL of labeled C1q prepared as above, andaccording to case with 5 lLof45lMfucoidan; when thepurified C1q was used, 400 lLof6.5pMT4 DNA weremixedwith10 lL of ... region are involved in the assembly of C1 throughspecific interaction with acidic residues of C1r [35].Furthermore, the structural model of C1q shows a cluster of basic residues that are located ... labeled-CLR (CLR*).Incubation of T4 DNA with CLR* in the presence of fucoidan (CLR/fucoidan molar ratio of 1 : 20 and 1 : 40)leads to an inhibition of the binding of CLR* to DNA fromthe molar...
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Báo cáo khoa học: A spectroscopic study of the interaction of isoflavones with human serum albumin pdf
... visuali-zation. One mole of genistein is bound per mole of HSA with a bindingconstant of 1.5 ± 0.2 · 105m)1. Binding of genistein to HSA precludesthe attachment of daidzein. The ability of HSA to bind ... 1.Fluorescence quenching studies with genistein Interaction of genistein with HSA has been monitoredfollowing the quenching of relative fluorescence inten-sity of HSA. Quenching of fluorescence by genisteindoes ... some of these residues are likely tointeract depending upon the precise orientation andpositioning of the genistein within a broader predictedbinding site. The interactions of both ligands with albumin...
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Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
... thusdemonstrating specific binding of XPA (lane 2, band 2). With increasing concentrations of selenocystine (lanes3–10), the intensity of band 2 decreased. Nevertheless,instead of free oligonucleotide, ... present inone of the major classes of transcription factors and otherso-called zinc finger proteins. Within this study we investi-gated the effect of selenium compounds on the activity of theformamidopyrimidine-DNA ... [34].Determination of the metal content of MT by complete zincrelease after oxidation with 10 mMH2O2and ICP-MSrevealed 6.4 zinc atoms per molecule of MT and negligibleamounts of cadmium and...
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