interaction of electron with matter pdf

... for the interaction of frog annexin A1 and annexin A2 with dicalcin. Consistently, we observed that the 35 kDa forms of annexin A1 and annexin A2 found in the fraction of the binding proteins of dicalcin (Fig. ... presence of dicalcin and all of the annexins (E) was compared with the calcu- lated sum of each of the initial rates obtained in (B)–(D) (thick dot- ted lines). T. Uebi et al. Role of dicalcin ... 4869 Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia Tatsuya Uebi 1 , Naofumi Miwa 1,2, * and Satoru Kawamura 1,2 1 Department of Biology, Graduate School of Science,...

Ngày tải lên: 07/03/2014, 05:20

Ngày tải lên: 12/08/2014, 03:21

... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of ... 2002 Interaction of decorin with collagen peptides (Eur. J. Biochem. 269) 1431 with respect to the unmodified ones is not related to the percentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin. DISCUSSION The binding of decorin with fibrillar collagens has been extensively...

Ngày tải lên: 21/02/2014, 15:20

... the interaction of TATA box binding protein and TFIIA with DNA. Biochemistry 33, 7033–7040. 4760 E. Trotta et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Interaction of DAPI with individual strands of ... compact structure of DAPI-bound ATT- template with respect to its free form, which is consistent with the previously reported lower mobility of the free monomeric random coil structure of (ATT) 6 in ... primer-complementary region of the template. (C) Chemical structure of DAPI. 4756 E. Trotta et al. (Eur. J. Biochem. 270) Ó FEBS 2003 the interaction with DAPI. This suggests an efficient mechanism of single-stranded...

Ngày tải lên: 16/03/2014, 23:20

... indicating that the interactions of these differ- ent proteins are likely to be indirect, depending on the presence of intact RNA as integral component of the RNP. Association of 42Sp50 with mRNA has ... that are formed with the corresponding signal sequences in the untranslated regions of the mRNAs and with a number of different so-called localization proteins. In the context of the present study, ... over- night at 4 °C. If indicated, 5 lL of RNase A (10 mgÆmL )1 ) was added together with the antibody. Immunopellets were washed with ice-cold IPP145, mixed with 30 lLof2· SDS loading dye and analyzed...

Ngày tải lên: 23/03/2014, 03:20

... barely interacts with the region of H1 and the O-methyl group. The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galac- tose residue of lactose formed hydrogen bonds with EW29Ch, ... tighter interaction with lac- tose than the c sugar-binding site because of the num- ber of intermolecular hydrogen bonds and of residues interacting with lactose. Our results agreed well with those ... structure of EW29Ch with lactose showed that the glucose residue of the lactose molecule interacted with subdomain c of EW29Ch [25]. This interaction may be an artifact caused by the crystallization of...

Ngày tải lên: 23/03/2014, 04:21

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Ngày tải lên: 08/08/2014, 19:21

Ngày tải lên: 09/08/2014, 06:22

... ) * k on;1 k off;1 AB ð2Þ AB þ B ) * k on;2 k off;2 AB 2 ð3Þ where d½B=dt ¼À2k on;1 ½A½Bþk off;1 ½ABÀk on;2 ½AB[B] þ 2k off;2 ½AB 2 ð4Þ d½AB=dt ¼ 2k on;1 ½A½BÀk off;1 ½AB À k on;2 ½AB[B] þ 2k off;2 ½AB 2 ð5Þ d½AB 2 =dt ... that mutation of Ca 2+ ligating amino acids in the N-terminal part of the first EGF-like domain of factor X influences the amidolytic activity of the intact protein [36]. However, direct interactions ... vicinity of the membrane. We found that the isolated factor X Gla domain exhibits low affinity binding to PtdSer-containing membranes with a K d of 4.6 l M . This agrees well with the value of 2.4...

Ngày tải lên: 08/03/2014, 23:20

... behavior of trehalase depending on the nature of the activation stress (Fig. 5B). This shift might argue against the participation of a unique set of interactions involving association of Ntp1p ... identification of strains C3 and C69, with one copy of Ntp1p–Ha6H expressed from the genomic ntp1 + promoter, was verified by Southern blot analysis and immunoblot of whole-cell extracts with anti- Ha ... the regulation of Ntp1p activity under stress relies on the existence of some kind of interaction between Ntp1p and Tps1p, or other elements, that may be lost under the conditions of the in vitro...

Ngày tải lên: 08/03/2014, 23:20

... with the 3¢ of the )148 to )124 region of c-jun. However, this interaction was not found to be absolutely essential f or its interaction with RLjunRP c omplexed with Jun-25. The interaction of rRLjunRP ... groove of the )148 to )124 region of c-jun To establish the nature of interaction b etween trans-acting factor(s) and t he )148 to )124 r egion of c-jun,drugsthat specifically interact with the ... site of of factors int eracting with the )148 to )124 region of c-jun. (A,B) Effect of distamycin A (Dist. A) on complex f ormation. Standard EMSA reactions were carried o ut us ing 1 ng of labelled...

Ngày tải lên: 16/03/2014, 18:20

... lLof6.5p M T4 DNA were mixedwith1lL of labeled C1q prepared as above, and according to case with 5 lLof45l M fucoidan; when the purified C1q was used, 400 lLof6.5p M T4 DNA were mixedwith10 lL of ... region are involved in the assembly of C1 through specific interaction with acidic residues of C1r [35]. Furthermore, the structural model of C1q shows a cluster of basic residues that are located ... labeled-CLR (CLR*). Incubation of T4 DNA with CLR* in the presence of fucoidan (CLR/fucoidan molar ratio of 1 : 20 and 1 : 40) leads to an inhibition of the binding of CLR* to DNA from the molar...

Ngày tải lên: 16/03/2014, 23:20

... visuali- zation. One mole of genistein is bound per mole of HSA with a binding constant of 1.5 ± 0.2 · 10 5 m )1 . Binding of genistein to HSA precludes the attachment of daidzein. The ability of HSA to bind ... 1. Fluorescence quenching studies with genistein Interaction of genistein with HSA has been monitored following the quenching of relative fluorescence inten- sity of HSA. Quenching of fluorescence by genistein does ... some of these residues are likely to interact depending upon the precise orientation and positioning of the genistein within a broader predicted binding site. The interactions of both ligands with albumin...

Ngày tải lên: 23/03/2014, 11:20

... thus demonstrating specific binding of XPA (lane 2, band 2). With increasing concentrations of selenocystine (lanes 3–10), the intensity of band 2 decreased. Nevertheless, instead of free oligonucleotide, ... present in one of the major classes of transcription factors and other so-called zinc finger proteins. Within this study we investi- gated the effect of selenium compounds on the activity of the formamidopyrimidine-DNA ... [34]. Determination of the metal content of MT by complete zinc release after oxidation with 10 m M H 2 O 2 and ICP-MS revealed 6.4 zinc atoms per molecule of MT and negligible amounts of cadmium and...

Ngày tải lên: 30/03/2014, 15:20

Ngày tải lên: 31/03/2014, 15:20

Ngày tải lên: 31/03/2014, 21:21

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Ngày tải lên: 11/08/2014, 00:22

Ngày tải lên: 12/08/2014, 03:20