interaction of carbon chain polymers with no2

... the active sites of this enzyme and arginase I In spite of the relatively low agmatinase activity of the Asn149Asp variant, it is clear that the interactions of arginase II with l-arginine and ... blue staining of purified enzymes Metal-free species of purified enzymes were obtained by incubation for h at 25 °C with 25 mm EDTA and m 4545 ´ V Lopez et al Interaction of arginase II with substrate ... specificity of arginase II, yielding enzyme species with almost undetectable activity on arginine but significant activity on agmatine From the effects of replacement of His120 and His145 with asparagine...

Ngày tải lên: 20/02/2014, 02:21

... coimmunoprecipitated with the ETAR-1D4, regardless of the absence or presence of ET-1 (lanes and 3), but not without the expression of the receptor (lane 1) This observation is consistent with those of the ... manner To examine the interaction of ETBR with caveolin-1, we took advantage of an insect cell expression system, in which ETAR and ETBR are expressed well, with KD values for ET-1 of 55 ± and 83 ± ... conformational changes of ETBR affect this interaction, and the ground-state structure of ETBR is required for the interaction with caveolin-1 The discrimination by caveolin-1 of the RES-701-1bound...

Ngày tải lên: 17/03/2014, 03:20

... Suppression of HIV-1 viral replication by Hsp16 requires C-terminal end of Vpr x 106 to x 106 of hsp16-expressing H9 or CEM-SS cells were either mock infected or infected with 2.0 x 103 TCID50 of HIV-1LAI ... supernatants To neutralize the effect of potential contamination with endotoxin [45], 10 μg/ml of Polymyxin B (PMB) was added [45] Results are mean ± SE of triplicates Page of 14 (page number not for citation ... inability of Vpr' to compete with Hsf-mediated Hsp16 elevation Thus, assuming that expression of hsp16 is responsive to both the presence of Vpr and heat shock treatment, this larger increase of Hsp16...

Ngày tải lên: 13/08/2014, 09:20

... 2.2.1 Purification of the N‐terminal domain of Tim44  15  2.2.2 Structure of the N‐terminal domain of Tim44  16  2.2.3 Purification of the NBD and PBD of Ssc1  18  2.2.4 Secondary structure of the NBD and PBD of Ssc1  ... 1.2 The pathway of mitochondrial matrix protein translocation  2  1.3 The regulated cycling of Ssc1 by its interaction with Tim44  6  1.4 LID sub‐domain  9  1.5 Objective  10  Chapter 2    Study of the interaction of Tim44 N‐terminal domain and different  ... conformation  with high  on  and  off  substrate  binding  rates.  In  contrast,  the  ADP state of NBD corresponds to a close state of PBD with high affinity to the  substrate  and  low  on  and  off ...

Ngày tải lên: 08/11/2015, 16:31

... initial carbon content, it dropped to nearly 25% at the end of the composting period (Figure 4) Decrease in total organic carbon concentration resulted from the oxidation of carbon to carbon dioxide ... during composting of cassava pulp with swine manure After 18 days of composting, the pH of all piles increased and pH levels remained relatively unchanged after Day 35 The increase of pH in composting ... composting of cassava pulp with swine manure Total organic carbon and total nitrogen During the composting process, total organic carbon in all piles sharply decreased in the first 28 days of composting...

Ngày tải lên: 05/09/2013, 09:08

... evaluation of the kinetic parameters in the presence of stearate Evaluation of kinetic parameters Fig Comparison of three models for the catalytic action of plasmin in the presence of stearate ... Light attenuation of oleic acid micelles in the presence of synthetic plasmin substrate (A) Twenty microlitres of mM Spectrozyme-PL was added to 180 lL of various concentrations of oleic acid (the ... course of activity assays As illustrated in Fig 5, this test indicated minimal loss of plasmin activity in the absence of fatty acids (Fig 5A) compared with the markedly lower levels of end-stage...

Ngày tải lên: 18/02/2014, 17:20

... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the light ... intensity of the band of unhydrolyzed actin was plotted against the time of incubation Characterization of actin preparation quality Fluorescence parameters were used for estimation of nativity of actin ... parameter A of actin was close to 2.4 Increase of the time of heating at 43 °C leading to decrease of parameter A up to 2.2–2.3 was accompanied by further decrease of the initial rate of polymerization...

Ngày tải lên: 20/02/2014, 23:20

... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of ... methylation, respectively Our results indicate that elimination of the positive charge of the side chains of Lys/Hyl residues disrupts the interaction with decorin (Fig 6) This does not depend on the derivatizing ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin DISCUSSION The binding of decorin with fibrillar collagens has been extensively...

Ngày tải lên: 21/02/2014, 15:20

... equilibrated with 10 column volumes (10 · 0.2 mL) of buffer B, with subsequent washing four times with five volumes of the same buffer Proteins were eluted with buffer E (buffer B supplemented with 250 ... for nonspecific interactions Figure 2A shows a response difference sensorgram (FC2 – FC1) of interactions of GlnK with immobilized TnrA For this analysis, an analyte concentration of 40 nm GlnK ... and incubated with 0.5 mL of antiserum for h at 24 °C with gentle shaking After being washed times with mL of 0.2 m Na3BO3 (pH 9.0), the Sepharose beads were resuspended in mL of 0.2 m Na3BO3...

Ngày tải lên: 06/03/2014, 00:21

... with the cDNA probes to isolate the full-length cDNAs of annexins Expression of annexins in E coli The coding region of each annexin was amplified by PCR with a pair of primers The sequences of ... mass of 38 kDa with pI values of 6.2–7.1 (annexin A1), and of c (annexin A2), and a single spot at 35 kDa with pI ¼ 5.6 (annexin A5) Each annexin subtype is indicated by a circle Colocalization of ... Immunofluorescence double-staining of one subtype of annexin with the other subtype of annexin A section was treated with rabbit antiserum raised against one subtype of annexin (red: A, annexin A1;...

Ngày tải lên: 07/03/2014, 05:20

... analysis The interaction of soluble b chains with SAgs was monitored in a resonance mirror with an IAsys instrument (Labsystem, Cambridge, UK) biosensor, which allows determination of real time interactions ... lower Kd The Kd calculation is independent of the amount of Ó FEBS 2004 Interaction of human TCR with superantigen SSA (Eur J Biochem 271) 4081 Fig Vb1–SSA interaction analysis Association curves ... the binding of Vb1–SSA with Kd ¼ 10.82 lM (E) and Vb1–SSA(C26S) with a Kd ¼ 9.14 lM (F) Fig SSA dimer in supernatant of S pyogenes Supernatants of isolates from patients infected with S pyogenes...

Ngày tải lên: 07/03/2014, 16:20

... contribution of electrostatic interactions to the binding of both peptides with negatively charged SUVs, titration of the peptides with PtdCho ⁄ PtdSer vesicles was carried out in the absence of salt ... report on the interaction of an N-terminal (E2(7–26)) and an internal (E2(279–298)) synthetic peptide sequence of the E2 structural protein of HGV ⁄ GBV-C with phospholipid membranes of different ... presence of PamOlePtdCho ⁄ PamOlePtdGro SUVs The electrostatic interactions were further studied by titration of the peptides with egg PtdCho ⁄ brain PtdSer (65 ⁄ 35) SUVs in Tris ⁄ HCl buffer without...

Ngày tải lên: 07/03/2014, 17:20

... study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers Interaction of stefin B in the prefibrillar aggregated state with model lipid membranes ... channel hypothesis of AD and neurodegeneration in general, is not incompatible with other key elements of toxicity, as, for example, the deregulation of Ca2+ homeostasis and generation of reactive oxygen ... stefin B which is more basic, with an isoelectric point of ~ 8, than stefin A, with an isoelectric pont of ~ An additional factor may be the much higher stability of stefin A which also may count...

Ngày tải lên: 07/03/2014, 17:20

... and ABP96, but no interaction with ABP64 (Table 1) Ó FEBS 2002 Calliphora anterior fat body protein (Eur J Biochem 269) 957 Table Interaction of AFP with different fragments of the hexamerin receptor ... predicted protein is composed of 306 amino acids, with a calculated molecular mass of 34.3 kDa and a pI of 5.72 Similar searches with the deduced amino-acid sequence of full-length Calliphora AFP, ... results of the immuno-coprecipitation revealed that AFP interacts with P30 and with all cleavage products of the hexamerin Expression of AFP in C vicina As in almost all experiments dealing with...

Ngày tải lên: 08/03/2014, 22:20

... AB koff;1 kon;2 AB þ B )* AB2 koff;2 ð2Þ ð3Þ where d½BŠ=dt ¼ À 2kon;1 ½AŠ½BŠ þ koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2 ½AB2 Š ð4Þ d½ABŠ=dt ¼ 2kon;1 ½AŠ½BŠ À koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2 ... vicinity of the membrane We found that the isolated factor X Gla domain exhibits low affinity binding to PtdSer-containing membranes with a Kd of 4.6 lM This agrees well with the value of 2.4 lM ... that mutation of Ca2+ ligating amino acids in the N-terminal part of the first EGF-like domain of factor X influences the amidolytic activity of the intact protein [36] However, direct interactions...

Ngày tải lên: 08/03/2014, 23:20

... behavior of trehalase depending on the nature of the activation stress (Fig 5B) This shift might argue against the participation of a unique set of interactions involving association of Ntp1p ... the regulation of Ntp1p activity under stress relies on the existence of some kind of interaction between Ntp1p and Tps1p, or other elements, that may be lost under the conditions of the in vitro ... identification of strains C3 and C69, with one copy of Ntp1p–Ha6H expressed from the genomic ntp1+ promoter, was verified by Southern blot analysis and immunoblot of whole-cell extracts with antiHa...

Ngày tải lên: 08/03/2014, 23:20

... investigate the effect of pH on MP–NV interaction Previously, it was shown that SeMV particles are stable over a pH range of 3–10.4 Fig Interaction of rMP with NV (A) ELISA of rMP and NV interaction ELISA ... FEBS 263 Interaction of SeMV MP with viral coat protein S R Chowdhury and H S Savithri A B Fig Mapping of the SeMV MP domain responsible for interaction of MP with NV (A) Purification of GST–MP ... of the biochemical nature of the interaction between rMP and NV (A) Effect of rMP concentration on the NV–MP interaction NV-coated ELISA plates were incubated with increasing concentrations of...

Ngày tải lên: 15/03/2014, 00:20

... were washed twice with buffer C and twice with buffer C without NP40 The elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl, incubating for 10 with gentle stirring ... nuclear-enriched fraction of proteins The efficiency of labelling of the single oligomers being not homogenous, the ratios of the different binding signals within each lane instead of their absolute values ... the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer Therefore, a fixed aliquot of the protein was incubated with ng of the indicated...

Ngày tải lên: 16/03/2014, 13:20

... correlate with the binding affinity between GA and the receptor 6158 The thermodynamic parameters obtained are in the range of those of other interactions with c-CD [15] The interaction of GA with ... the addition of a- and b-CDs FEBS Journal 272 (2005) 6154–6160 ª 2005 FEBS Y Izutani et al Interaction of gymnemic acid with cyclodextrins Table Thermodynamic parameters of the interaction between ... binding of GA to the pocket of c-CD occurs in the aqueous solution, accompanying dispersion of the self-association of GA DLS analysis Fig 1H NMR spectra (500 MHz) of GA in the absence or presence of...

Ngày tải lên: 16/03/2014, 14:20

... interact with the 3¢ of the )148 to )124 region of c-jun However, this interaction was not found to be absolutely essential for its interaction with RLjunRP complexed with Jun-25 The interaction of ... Expression of c-jun in regenerating rat liver (Eur J Biochem 271) 4897 A Dist A (mM) Fig Determination of the binding site of of factors interacting with the )148 to )124 region of c-jun (A,B) Effect of ... be correlated with the appearance of the factor, rRLjunRP, involved in the formation of complex C2, observed with rRNE-d, and if the time of appearance of this factor coincides with the increase...

Ngày tải lên: 16/03/2014, 18:20