integrin mediated resistance to chemotherapy induced apoptosis in cancer cells

... failure in human OvCa The balance between survival and apoptotic signals determine a cell's sensitivity to chemotherapy Indeed, cancer cells develop resistance to chemotherapy by means of inactivating ... for 15 minutes After washing in PBS three times, the cells were incubated with 0.05% Tween-20 in PBS for 15 minutes After washing in PBS, the cells were incubated with TdT end-labelling cocktail ... end-labelling cocktail for 60 minutes Termination buffer was added to stop the reaction After washing times in PBS, cells were blocked for 20 minutes and stained with avidin-fluorescein isothiocyanate (FITC)...

Ngày tải lên: 20/06/2014, 07:20

... as the phosphoinositide 3-kinase (PI3-kinase) and the mitogen-activated protein kinase (MAP-kinase) signalling cascades, have been suggested to regulate death receptor -induced apoptosis [22,23] ... did not confer Fas resistance to P.U cells (Fig 6G), indicating that increased phosphorylation of ERK1 ⁄ is not sufficient to mediate the resistance to Fas -induced apoptosis In conclusion, this ... an array of proteins, e.g Flice-like inhibitory proteins (FLIPs), Bcl-2 family members, Fas-associated protein tyrosine phosphatase and inhibitors of apoptosis proteins [15] To establish which...

Ngày tải lên: 23/03/2014, 06:20

... cisplatin -induced cytotoxicity in cancer cells Discussion In this study we demonstrated that both SSa and SSd potently sensitize a number of human cancer cells to cisplatin -induced apoptosis through ... saikosaponin-a and -d could synergistically sensitize various cancer cells to cisplatin -induced cell death Saikosaponins and cisplatin co-treatment potentiates apoptosis in cancer cells Cisplatin can induce ... cancer cells to cisplatin induced cytotoxicity Both SSa and SSd have been reported to induce proliferation inhibition and cell death in various cancer cells (5-9) However, the effect of combination...

Ngày tải lên: 10/08/2014, 10:20

... shown In contrast to human CD97, murine CD97 lacks an RGD motif in the stalk region [27,28] which facilitates integrin binding in humans [10] The 1B2 mAb used in this study blocks binding to CD55 ... to chondroitin sulfate B in addition to blocking binding to CD55 Although 1B2 does not block chondroitin sulfate B binding in vitro [17], it could sterically hinder this interaction in vivo This ... with haematoxylin and eosin or safranin O fast green Inflammation was graded on a scale from (no inflammation) to (severely inflamed joint) based on infiltration by inflammatory cells in the synovium...

Ngày tải lên: 09/08/2014, 08:22

... aim of this study was to characterize global changes in the proteins related to apoptosis during hypoxia to clarify the mechanism of hypoxia -induced apoptosis, and to find out useful hypoxia-related ... pro- and antiapoptosis-related proteins were expressed in the process of hypoxia -induced apoptosis in the trophoblastic cell line JAR Mitochondrial pathway-related and stress reaction -induced pathways, ... proteins involved in apoptosis, including the expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig...

Ngày tải lên: 31/10/2012, 15:12

... kinase activity is critical for C2- and C8-ceramideinduced apoptosis in PC12 cells DISCUSSION DAP kinase is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in apoptosis induced ... mutant resulted in some growth disadvantage for PC12 cells No band was seen in PC12 cells transfected with pcDNA3 vector alone A HA-tag CaM binding Kinase domain Cytoskelton binding Ankyrin repeats ... K42A-DAP kinase mutant failed to induce apoptosis in the presence of ceramide, that the DCaM-DAP kinase mutant induced apoptosis even in the absence of ceramide, and that PC12 cells overexpressing...

Ngày tải lên: 24/03/2014, 00:21

... several LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor), and ... inhibits NSC-741909 -induced apoptosis Our previous studies have demonstrated that the reduction in cell viability caused by oncrasin compounds is mainly caused by apoptosis induction [2] To further evaluate ... cyclooxygenase inhibitor; oxypurinol, hypoxanthine/xanthine oxidase inhibitor; and NDGA, nordihydroguaiaretic acid, a lipoxygenase inhibitor decreased the percentage of apoptotic cells compared...

Ngày tải lên: 18/06/2014, 16:20

... generates binding sites for proteins containing Src homology-2 (SH2) and protein tyrosine-binding (PTB) domains4 Two families of tyrosine kinases exist within cells They are the receptor tyrosine kinases ... STAT5A Protein tyrosine-binding Polypyrimidine tract binding protein p53 upregulated modulator of apoptosis Rat sarcoma Splicing factor Src homology-2 Src homology-3 Small interfering RNAs Single nucleotide ... polymorphism are less sensitive to gefitinib -induced apoptosis …………………………………………………… 65 Addition of ABT-737 overcomes intrinsic resistance to gefitinib in polymorphism- containing PC9 cells …………………………………………...

Ngày tải lên: 10/09/2015, 09:04

... beneficial in chemotherapy Similar to taxol and vinblastine, benomyl also induces apoptosis in HeLa cells, indicating that benomyl may be useful in combination with other potent anticancer drugs ... protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Ramoneda BM & Perez-Tomas R (2002) Activation of protein kinase C for protection of cells against apoptosis induced ... microtubules to kinetochores (Fig 3A) In benomyl treated cells, some kinetochore pairs were found to be attached to kinetochore fibres from both the poles whereas others were attached to the kinetochore...

Ngày tải lên: 30/03/2014, 10:20

... subcutaneous late toxicity in our series This finding opens the possibility to develop new predictor assays taking into account the initial DNA damage and radiation -induced apoptosis levels, and introduces ... observed Initial DNA damage levels in breast cancer patients Radiation -induced apoptosis in breast cancer patients Initial DNA damage was determined as radiationinduced double-strand breaks (DSB) in ... and radiation -induced apoptosis with normal tissue toxicity As previously published, increasing numbers of radiation induced DSB were related to severe late toxicity in breast cancer patients...

Ngày tải lên: 09/08/2014, 09:20

... it has been shown to protect cells from H2O2 -induced apoptosis [19,20] In this study, we investigated H2O2 -induced apoptosis in Rat1 cells expressing an Evi1 transgene Surprisingly, we found that ... examined in this study Sensitization to H2O2 -induced apoptosis, determined by caspase catalytic activity, was seen in both Evi1expressing Rat1 cells (5.6 cells) and in caIII knockdown Rat1 cells ... oxidant -induced apoptosis 5.62 cells that is absent from Neo1, Neo2 and parental Rat1 cells (Fig 1B) Previous studies have shown that Evi1 is a survival factor, protecting cells from apoptosis induced...

Ngày tải lên: 29/03/2014, 08:20

... apoptosis induced by tumor necrosis factor-related apoptosisinducing ligand (TRAIL) [14,15], which selectively induces apoptosis in malignant, but not nonmalignant, cells [16] TRAIL binds to death ... of BRCA1 in HCC1937 cells resulted in a 22-fold enhancement in apoptosis relative to cells expressing BRCA1 in the absence of TRAIL and a three-fold enhancement of apoptosis over that induced ... TRAIL, but not hypoxia, induced apoptosis, and TRAIL -mediated apoptosis was enhanced by BRCA1 Results Hypoxia induces nuclear accumulation of BRCA1 in breast cancer cells To investigate the effects...

Ngày tải lên: 30/03/2014, 03:20

... that the resistance to anti-Fas -induced apoptosis in lung fibroblasts is mediated by the overexpression of the specific inhibitors of apoptosis X-chromosome-linked inhibitor of apoptosis (ILP) ... short-lived inhibitory proteins and led to increased susceptibility to Fas -mediated apoptosis We used cycloheximide to analyse whether the differences in sensitivity to FasL -induced apoptosis depended ... were more resistant to FasL -induced apoptosis Incubation of the cells with TNF-α slightly increased the susceptibility of n-fibs to apoptosis, but it had no affect on apoptosis in f-fibs (Fig 1D)...

Ngày tải lên: 12/08/2014, 18:21

... Bleomycin alone in iNOSWT mice, extensive inflammation with inflammatory cells infiltration and fibrosis C: Bleomycin in iNOSKO mice patchy areas of inflammation with minimal fibrosis D: Bleomycin in ... staining for nitrotyrosine (B) was localized mainly in nuclei of inflammatory cells There was a marked reduction in the immunostaining in the lungs of bleomycin-treated iNOSKO mice (C) and in ... acting (5 hours half life in rats) iNOS inhibitor and is also able to inhibit LPS -mediated increase in plasma NO2- NO3- levels 14 h after single intraperitoneal dose (ED50 mg kg-1) [23] The inhibition...

Ngày tải lên: 12/08/2014, 18:21

... This resistance was usually restricted to HIV-1 isolates using the CCR5 chemokine receptor (R5 isolates) to enter target cells [12-14] Invalidating mutations in the CCR5 gene confer resistance to ... chemokine responses, we analyzed CCR5 -mediated actin polymerization in CD4 T cells from subjects W278 and B195 RANTES stimulation of CD4 T cells induced a rapid increase in the F-actin content of cells ... replication is likely to be determined in large part by the balance between required factors and restrictive factors CD4 T cell resistance to single-round HIV-1 infection in Vietnamese EUs In a previous...

Ngày tải lên: 13/08/2014, 09:20

... proteasome -induced apoptosis [16] Although the treatment of cells with this compound causes marked increases in a large number of cellular proteins, including cyclin-dependent protein kinase inhibitors, ... inhibitor and TRAIL markedly enhanced apoptosis in cultured canine chondrocytes To investigate the proteasome inhibition effect on TRAIL -induced apoptosis in canine chondrocytes, N-acetyl-Leu-Leu-Norleu-al ... Nuclear insulin-like growth factor binding protein-3 induces apoptosis and is targeted to ubiquitin ⁄ proteasome-dependent proteolysis Cancer Res 66, 3024–3033 Wu S & De Luca F (2006) Inhibition...

Ngày tải lên: 18/02/2014, 14:20

... factor superfamily, has been shown to be a promising candidate for novel anticancer therapies [4] Interestingly, TRAIL is capable of inducing apoptosis in a wide range of tumor cells, but not in ... ⁄ c-Jun signaling pathway contributes to DR5 induction and apoptosis induced by TRAIL plus PIs in glioma cells (A) Inhibition of JNK ⁄ c-Jun signaling downmodulates DR5 induction in a dose-dependent ... inhibition sensitizes hepatocellular carcinoma cells, but not human hepatocytes, to TRAIL Hepatology 42, 588–597 38 Kurland JF & Meyn RE (2001) Protease inhibitors restore radiation -induced apoptosis...

Ngày tải lên: 18/02/2014, 17:20

... al A Fos -mediated vanadium -induced apoptosis Fig VOSO4 induced apoptosis of HaCaT cells (A) HaCaT cells (1 · 105) were treated with increasing concentrations of VOSO4, ranging from to 1000 lM, ... A B C D E Fos -mediated vanadium -induced apoptosis Fig c-fos inhibited proliferation and induced apoptosis of HaCaT cells through changes in CLU expression (A) HaCaT cells were infected with high-titer ... other findings showing that p53 transactivation was required for vanadiuminduced apoptosis of mouse epidermal cells [8] A major factor that appeared to contribute to VOSO4 -induced apoptosis was...

Ngày tải lên: 16/03/2014, 02:20

... of apoptosis [16] Focal adhesion complexes consist of integrin proteins, the tyrosine kinases Src and focal adhesion kinase (FAK), actin-binding structural proteins, and the adaptor proteins ... adhesion proteins and PKCd The elucidation of intracellular mechanisms affected by lovastatin will contribute to gaining insight in the growth inhibition and apoptosis induced by this statin in neuroblasts, ... during apoptosis, examples include p21 (CDKN1A)-activated kinase (PAK2) [28], MAPK kinase kinase (MEKK-1) [29], MAPK kinase kinase kinase (Raf-1), protein kinase B (Akt) [30,31] and protein kinase...

Ngày tải lên: 23/03/2014, 11:20

... for Zn -induced apoptosis in human Burkitt lymphoma B cells An increase in labile Zn in the cytoplasm may suppress apoptosis by inhibition of the action of caspase, cytoplasmic apoptosis inducer, ... protein)1, indicating that the ability of Zn to induce apoptosis is dependent on the intracellular Zn level In fact, it has been shown that treatment with Zn (80–200 lM) induced apoptosis in mouse ... cell lines, were cultured in RPMI 1640 containing 10% fetal bovine serum in a 5% CO2 atmosphere Cytotoxicity of Py/Zn in HL-60 cells The cytotoxicity of Py/Zn in HL-60 cells was analyzed by colorimetric...

Ngày tải lên: 23/03/2014, 21:20