α mediates dheas induced apoptosis in vsmcs

... (data not shown) Int J Med Sci 2007, 39 Hypoxia -induced apoptosis in the JAR cell line, and Bag-1 Morpho/AS enhanced apoptosis of the cell line With hypoxic treatment for 24 hours, internucleosomal ... many proteins involved in apoptosis, including the expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig ... study was to characterize global changes in the proteins related to apoptosis during hypoxia to clarify the mechanism of hypoxia -induced apoptosis, and to find out useful hypoxia-related markers...

Ngày tải lên: 31/10/2012, 15:12

... during apoptosis, examples include p21 (CDKN1A)-activated kinase (PAK2) [28], MAPK kinase kinase (MEKK-1) [29], MAPK kinase kinase kinase (Raf-1), protein kinase B (Akt) [30,31] and protein kinase ... of apoptosis [16] Focal adhesion complexes consist of integrin proteins, the tyrosine kinases Src and focal adhesion kinase (FAK), actin-binding structural proteins, and the adaptor proteins ... PKCd The elucidation of intracellular mechanisms affected by lovastatin will contribute to gaining insight in the growth inhibition and apoptosis induced by this statin in neuroblasts, and additionally...

Ngày tải lên: 23/03/2014, 11:20

... protein)1, indicating that the ability of Zn to induce apoptosis is dependent on the intracellular Zn level In fact, it has been shown that treatment with Zn (80–200 lM) induced apoptosis in mouse ... for Zn -induced apoptosis in human Burkitt lymphoma B cells An increase in labile Zn in the cytoplasm may suppress apoptosis by inhibition of the action of caspase, cytoplasmic apoptosis inducer, ... aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that mediate intracellular signal transduction in response...

Ngày tải lên: 23/03/2014, 21:20

... the human DAP kinase mutant proteins used in these experiments The DCaM-DK mutant lacks 47 amino acid residues (proline 277 to leucine 323) containing the calmodulin-binding domain In the K42A-DK ... critical for C2- and C8-ceramideinduced apoptosis in PC12 cells DISCUSSION DAP kinase is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in apoptosis induced by a variety of signals ... kinase mRNA in brain is increased prior to cell death induced by transient forebrain ischemia [8] These ®ndings indicate a possible relationship between DAP kinase and neuronal cell death, including...

Ngày tải lên: 24/03/2014, 00:21

... cells from H2O2 -induced apoptosis [19,20] In this study, we investigated H2O2 -induced apoptosis in Rat1 cells expressing an Evi1 transgene Surprisingly, we found that Evi1 expression increases sensitivity ... particular signalling pathways, including TGFb [12,13], c-Jun N-terminal kinase (JNK) [2] and phosphoinositide-3-kinase ⁄ protein kinase B (PI3K ⁄ AKT) [4] Intervention of critical signalling molecules ... murine Evi1-expressing recombinant retroviral vector p50M5.6neo and production of Evi1 in virus-infected Rat1 fibroblasts (A) Viral long terminal repeats (LTR), the murine Evi1 gene, including...

Ngày tải lên: 29/03/2014, 08:20

... proteins involved in various cellular processes, including inhibition of apoptosis Hypoxia has been reported to inhibit apoptosis induced by tumor necrosis factor-related apoptosisinducing ligand ... hypoxia in MDA-MB-468 cells (Fig 2B), resulting in a 35% decrease in nuclear BRCA1 relative to the level in normoxic cells in the presence of 2E5 The decrease in C Fig Hypoxia -induced changes in BRCA1 ... Collectively, the data in Fig support the involvement of a TRAIL-dependent process in mediating hypoxiainduced changes in BRCA1 localization TRAIL, but not hypoxia, induces apoptosis in breast cancer...

Ngày tải lên: 30/03/2014, 03:20

... times, the cells were incubated with 0.05% Tween-20 in PBS for 15 minutes After washing in PBS, the cells were incubated with TdT end-labelling cocktail for 60 minutes Termination buffer was added ... for 20 minutes, followed by washing with PBS containing 0.1% Triton X-100 Endogenous peroxidase activity was quenched using 1% H2O2 in wash buffer The cells were incubated in antibody blocking buffer, ... growth inhibition of OVCAR-3 and SKOV3 cell lines caused by cisplatin in a CCR9-dependent fashion CCL25 -induced cisplatin resistance of OvCa cell lines Treatment of OVCAR-3 and SKOV-3 cell lines...

Ngày tải lên: 20/06/2014, 07:20

... Our findings of rapamycininduced caspase activation is coincident with Nottingham et al’s [28] result that rapamycin expressed activated caspase in spinal cord of rats Rapamycin-dependent increase ... protein level Rapamycin treatment induced significant increase in both Bax and Bid protein expression NNK treatment increased Bax protein expression in time-dependent manner, however, rapamycin ... distinguishing early apoptosis and necrosis Untreated cells were not stained with Annexin V and PI, suggesting that most of them were intact live cells NNK induced significant apoptosis in concentration-...

Ngày tải lên: 07/08/2014, 18:20

... examined TET treatment induced apoptosis and resulted in a decrease in Akt and ERK expression PI3K inhibition had no clear synergistic effect on tetradrine -induced apoptosis, however, ERK inhibition ... 10-14% in the USA [11] Therefore, it is clear that Synergistic effect of ERK inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells Fig The effects of a PI3K/Akt inhibitor ... inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells 25 Fig Flowcytometric detection of apoptosis of A549 cells treated with tetrandrine (TET) Cells were incubated with 30...

Ngày tải lên: 07/08/2014, 23:22

... analyze radio -induced apoptosis of peripheral blood lymphocytes in head and neck cancer patients and explore their role in predicting radiation induced toxicity Methods Seventy nine consecutive ... Results Radio -induced apoptosis (RIA) could be defined as the percentage of total PBLs death induced by the radiation dose minus the spontaneous cell death (control, Gy) RIA values increased with ... Hernandez LA, Lara PC, Pinar B, Fontes F, Rodriguez Gallego C, Lloret M: Prediction of clinical toxicity in localized cervical carcinoma by radio -induced apoptosis study in peripheral blood lymphocytes...

Ngày tải lên: 09/08/2014, 10:20

... granular contents into the surrounding extracellular space, with subsequent inflammation [30-32] To gain insight into the mechanism of arsenic trioxide (ATO) -induced apoptosis, we examined the modulation ... bovine serum (FBS), and phosphate buffered saline (PBS) were obtained from Sigma Chemical Company (St Louis, MO) Annexin V fluorescein isothiocyanale (FITC) kit (contains annexin V FITC, binding ... transcription factor involved in cell cycle arrest or apoptosis, and modulation of cyclin D1 and cyclin A involved in cell cycle progression [18] Preclinical studies from our laboratory have also indicated...

Ngày tải lên: 10/08/2014, 22:21

Ngày tải lên: 11/09/2015, 09:18

... TTG IN GTP DEPLETION INDUCED APOPTOSIS OF INSULIN-SECRETING CELLS 48 3.1.1 Increase of in situ tTG activity and induction of apoptosis by MPA in HIT cells 48 3.1.2 Effects of tTG inhibitors ... and apoptosis of insulin secreting cells 23 1.6 AIM OF THIS STUDY .25 1.6.1 Does tTG participate in MPA induced β-cell apoptosis? 25 1.6.2 Is any caspase involved in MPA induced ... guanosine reverses p53 increment in apoptosis induced by ischemic injury in tubular cells, whereas a specific inhibitor of p53 (Pifithrin-alpha) mimics the effects of guanosine to prevent apoptosis...

Ngày tải lên: 17/09/2015, 17:18

... plays an important role in protection of neuronal cells against stress -induced apoptosis [26] In accordance with these findings, ischemia/reperfusion in the rat forebrain induces the expression ... of p38 induced by oxidative stress, we further examined whether its activation is responsible for the induction of apoptosis in F9 cells using SB202190, a potent inhibitor of p38 As shown in Fig ... using Superfect reagent (Qiagen) according to the manufacturer’s instructions Forty-eight hours after transfection, the cells were maintained in complete medium containing 400 lgÆmL)1 geneticin...

Ngày tải lên: 21/02/2014, 03:20

... H2O2 -induced apoptosis by using HeLa cells in which expression of Hsp105a or Hsp105b was regulated using doxycycline Our results suggest Hsp105a and Hsp105b suppress H2O2 -induced apoptosis by inhibition ... mitochondria in apoptosis Genes Dev 13, 1899–1911 Suppression of H2O2 -induced apoptosis by Hsp105 36 Fan M, Goodwin M, Vu T, Brantley-Finley C, Gaarde WA & Chambers TC (2000) Vinblastineinduced phosphorylation ... effect in embryonic F9 cells [17,20] However, it is not fully understood how Hsp105a regulates H2O2 -induced apoptosis In addition, the role of Hsp105b in stress -induced apoptosis remains to be...

Ngày tải lên: 16/03/2014, 06:20

... Interleukin 10 Interleukin Interleukin (interferon, beta 2) Interleukin 16 (lymphocyte chemoattractant factor) Interleukin 1, beta Mitogen-activated protein kinase 14 Mitogen-activated protein ... Mitogen-activated protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related C3 botulinum toxin substrate ... (GTP binding protein, 25kda) Wiskott–Aldrich syndrome-like Wiskott–Aldrich syndrome protein interacting protein v-raf murine sarcoma viral oncogene homolog B1 Mitogen-activated protein kinase kinase...

Ngày tải lên: 22/03/2014, 16:20

... and apoptosis was evaluated (D) *P < 0.05, **P < 0.01, (n = 3) participated in the VD3 -induced apoptosis in gastric cancer cells indicated that vitamin D induced PTEN expression through binding ... deacetylase inhibitor trichostatin a potentiates doxorubicin -induced apoptosis by up-regulating PTEN expression Cancer 109, 1676– 1688 Finnin MS, Donigian JR, Cohen A, Richon VM, Rifkind RA, Marks ... were therefore interested in determining whether TSA ⁄ NaBu had any effects on promoting the binding of Egr-1, a major transcription factor of PTEN To this, we first examined the in uences of TSA...

Ngày tải lên: 22/03/2014, 21:20

... prolonged growth in progressively increasing concentrations of stimulating Fas antibody [16] To investigate the molecular basis that underlies the resistance to Fas -induced apoptosis, the resistant ... silencing through CpG methylations does not account for the resistance to Fas -induced apoptosis in our system Survival signalling pathways downstream of Ras, such as the phosphoinositide 3-kinase ... phosphoinositide 3-kinase (PI3-kinase) and the mitogen-activated protein kinase (MAP-kinase) signalling cascades, have been suggested to regulate death receptor -induced apoptosis [22,23] Before we...

Ngày tải lên: 23/03/2014, 06:20

... of PKCh in the TCDD -induced AhR-independent signal transduction mechanism involved in L-MAT cell apoptosis Results Inhibition of TCDD -induced L-MAT cell apoptosis by rottlerin, an nPKC inhibitor ... described in 911 PKCh in TCDD -induced signaling for apoptosis the attached protocol The resin was washed once with 0.5 mL of 0.1 m glycine HCl, pH 3.5, for min, while thoroughly tapping and inverting ... [9,10] In previous studies, we attested that AhR cannot be involved in TCDD -induced apoptosis in the human T-cell line, L-MAT, because AhR does not exist in L-MAT [7,8] In view of our previous findings...

Ngày tải lên: 23/03/2014, 13:20

... ROS-mediated inhibition of MKPs is critical for TNFα -induced sustained activation of JNK and subsequent apoptosis [7] Interestingly, ROS were recently identified as common mediators of antibiotic -induced ... decreased protein levels of MKP1, one of MAP kinase phosphatases that inactivate JNK and p38 MAP kinases [2] Interestingly, NSC-741909 induced an increase of MKP1 mRNA expression in both time- ... stress NDGA inhibits NSC-741909 -induced apoptosis Our previous studies have demonstrated that the reduction in cell viability caused by oncrasin compounds is mainly caused by apoptosis induction...

Ngày tải lên: 18/06/2014, 16:20