drought induced responses in plant cells

... almost no increase in the phosphorylated JNKs protein level in the cell line Twenty-four-hour exposure to hypoxia resulted in a Int J Med Sci 2007, 42 marked increase (1.8-fold of control) in the ... Morpho/AS was examined using a fluorescent microscope Fluorescence was mainly observed in cytosol areas in transfected cells FITC-labeled Bag-1 Morpho/AS was transfected into almost all JAR cells by EPEI-mediated ... and methods Cell line and cell cultures The human choricarcinoma cell line JAR was obtained from American Type Culture Collection (Manassas, VA, USA) Cells were maintained in RPMI 1640 medium...

Ngày tải lên: 31/10/2012, 15:12

... variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proinflammatory cytokines, inhibition of insulin signaling, induction of endoplasmic ... Pharmigen, San Diego, CA, USA) binding and PI staining were performed according to [44] Following incubation with 1.0 lgÆmL)1 annexin V and 4.0 lgÆmL)1 PI for 20 min, cells were analyzed by flow cytometry ... mitochondrial fraction following stearate administration (Fig 3B) The presence of oleate inhibited this translocation, indicating the involvement of Bax activation in stearate -induced mitochondrial...

Ngày tải lên: 14/02/2014, 22:20

... metabolized Invertases (INVs) can in uence plant growth and development either directly (e.g by in uencing source–sink relationships or by acting as a true regulatory protein) or indirectly via ... Cytokinins, on the other hand, can delay plant senescence, and studies with the AtHXK1 mutant glucose insensitive show that sugars and cytokinins work antagonistically [19] Interestingly, cytokinin -induced ... nitric oxide in drought- induced abscisic acid synthesis in root tips of wheat seedlings Aust J Plant Physiol 28, 1055– 1061 Xiong L, Schumaker KS & Zhu J (2002) Cell signaling during cold, drought, ...

Ngày tải lên: 15/03/2014, 11:20

... knowledge concerning the regulatory complex in animal cells, there is very little comparable information about plant cells It remains a challenge to understand how metabolism can be regulated in a complex ... flanking sequences are conserved in mammalian E1a primary sequences Stoichiometric phosphorylation of any individual site resulted in total inactivation, but indicated that there were differences in ... expression in flowers might instead reflect mRNAs present in the pollen Mitochondrial PDC Product inhibition As with their mammalian and microbial counterparts, plant PDCs employ a multisite ping-pong kinetic...

Ngày tải lên: 17/03/2014, 09:20

... calmodulin-binding domain In the K42A-DK mutant, a lysine residue at position 42 in the kinase domain (asterisk) is replaced by an alanine The DD-DK construct contains the death domain and the serine-rich ... kinase activity is critical for C2- and C8-ceramideinduced apoptosis in PC12 cells DISCUSSION DAP kinase is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in apoptosis induced ... mutant resulted in some growth disadvantage for PC12 cells No band was seen in PC12 cells transfected with pcDNA3 vector alone A HA-tag CaM binding Kinase domain Cytoskelton binding Ankyrin repeats...

Ngày tải lên: 24/03/2014, 00:21

... described in (A) induced by saikosaponin and cisplatin co-treatment, we first detect morphological changes in saikosaponin and cisplatin-cotreated HeLa cells by acridine orange/ethidium bromide staining ... protects against CCl4 -induced liver injury in rats [24], their roles in intracellular redox modulation have never been addressed To investigate the mechanism of the saikosaponins and cisplatin -induced ... cytotoxicity induced by saikosaponins plus cisplatin in Siha cells, A549 cells, and SKOV3 cells Siha cells (A), A549 cells (B), and SKOV3 cells (C) were pretreated with NAC (1 mM) for 30 or remained...

Ngày tải lên: 10/08/2014, 10:20

... underlying Inhibitory Effect of Fucoidan on Cytokine -Induced Inflammation in Glioma Cells 2008 DO THI THU HANG Molecular Mechanisms nderlying Inhibitory Effect of Fucoidan on Cytokine -Induced Inflammation ... regarding its exact role in this regard in glial and non-glial cells In a study with galactosylsphingosine in rat astroglial cells, AP-1 plays a positive role in maintaining high level of iNOS ... fucoidan inhibits IFN-γ -induced iNOS expression in C6 cells via inhibition of JAK/STAT and p38 activation In contrast, in Raw264.7, fucoidan increases IFN-γ -induced iNOS via increase of TNF-α production...

Ngày tải lên: 13/11/2014, 06:42

... inactive [49] In our experiments NDGA was effective in inhibiting the activity of purified recombinant MTE-I in an in vitro assay performed in a Hepes-based buffer containing only KCl Information ... known that an acyl-CoA binding protein (ACBP) known also as DBI (diazepam binding inhibitor) is expressed in high concentrations in specialized cells such as steroid producing cells of the adrenal ... performed in triplicate activity in vitro Figure shows that a 5-min preincubation of recombinant protein with NDGA inhibited thioesterase activity Interestingly, NDGA produced only a 20% inhibition...

Ngày tải lên: 08/03/2014, 09:20

... H2O2 -induced apoptosis by using HeLa cells in which expression of Hsp105a or Hsp105b was regulated using doxycycline Our results suggest Hsp105a and Hsp105b suppress H2O2 -induced apoptosis by inhibition ... apoptosis induced by heat shock in HeLa cells HeLa-tet ⁄ Hsp105 cells were heated at 45 °C for 15–60 min, and then the cells were further incubated at 37 °C for h Cell viability (A), phosphatidylserine ... suppressed in cells overexpressing Hsp105a (Fig 5A) In addition, when the cells were fractionated into a soluble fraction and a particulate fraction containing mitochondria by treatment with digitonin,...

Ngày tải lên: 16/03/2014, 06:20

... Interleukin 10 Interleukin Interleukin (interferon, beta 2) Interleukin 16 (lymphocyte chemoattractant factor) Interleukin 1, beta Mitogen-activated protein kinase 14 Mitogen-activated protein ... Mitogen-activated protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related C3 botulinum toxin substrate ... (GTP binding protein, 25kda) Wiskott–Aldrich syndrome-like Wiskott–Aldrich syndrome protein interacting protein v-raf murine sarcoma viral oncogene homolog B1 Mitogen-activated protein kinase kinase...

Ngày tải lên: 22/03/2014, 16:20

... aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that mediate intracellular signal transduction in response ... cell lines, were cultured in RPMI 1640 containing 10% fetal bovine serum in a 5% CO2 atmosphere Cytotoxicity of Py/Zn in HL-60 cells The cytotoxicity of Py/Zn in HL-60 cells was analyzed by colorimetric ... protein)1, indicating that the ability of Zn to induce apoptosis is dependent on the intracellular Zn level In fact, it has been shown that treatment with Zn (80–200 lM) induced apoptosis in mouse...

Ngày tải lên: 23/03/2014, 21:20

... hypoxia in MDA-MB-468 cells (Fig 2B), resulting in a 35% decrease in nuclear BRCA1 relative to the level in normoxic cells in the presence of 2E5 The decrease in C Fig Hypoxia -induced changes in BRCA1 ... proteins involved in various cellular processes, including inhibition of apoptosis Hypoxia has been reported to inhibit apoptosis induced by tumor necrosis factor-related apoptosisinducing ligand ... the data in Fig support the involvement of a TRAIL-dependent process in mediating hypoxiainduced changes in BRCA1 localization TRAIL, but not hypoxia, induces apoptosis in breast cancer cells As...

Ngày tải lên: 30/03/2014, 03:20

... containing CCh, which induced a second calcium increase and a further increase in Ins(1,4,5)P3 (Fig 3Aa) Both of these calcium increases were accompanied by increased Ins(1,4,5)P3, indicating ... Calcium-dependent enhancement of receptorinduced Ins(1,4,5)P3 increases in CHO cells expressing m1 mAChR PC12h cells express m1 mAChR and CCh -induced increases in calcium and investigate the cell-type dependence ... degree of receptor -induced Ins(1,4,5)P3, it enhanced Ins(1,4,5)P3 induced by the reintroduction of extracellular calcium (Fig 6Ab) The receptor -induced level of Ins(1,4,5)P3 in cells not pretreated...

Ngày tải lên: 30/03/2014, 03:20

... several LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor), ... decreased protein levels of MKP1, one of MAP kinase phosphatases that inactivate JNK and p38 MAP kinases [2] Interestingly, NSC-741909 induced an increase of MKP1 mRNA expression in both time- ... Results NSC-741909 induced MKP1 and MKP7 clustering and generation of reactive oxygen species in oncrasin-sensitive cells Our recent study showed that NSC-741909 induced sustained JNK activation...

Ngày tải lên: 18/06/2014, 16:20

... G0/G1 phase into S phase, and induced cells toward apoptosis Next, using similar method, we investigated the effects of p3b/EGFP-N1 on cell cycles in 293 and Vero cells In these cells, the p3b/EGFP-N1 ... apoptosis induction of 3b protein might be a cell type specific In order to further confirm the function of 3b protein in inducing cell apoptosis, a more definitive study using double cell labeling ... signaling by hepatitis B virus-X protein in liver cells Exp Mol Med 2003, 35:301-309 Nakamichi K, Matsumoto Y, Otsuka H: Bovine herpesvirus U(S) ORF8 protein induces apoptosis in infected cells...

Ngày tải lên: 19/06/2014, 08:20

... to interfere with TLR3 signaling in infected cells, and possibly has a viral TLR3 inhibitor HSV can also activate signaling pathways of innate immunity in infected cells, as its UL37 protein ... Figure U937 cells IRF-3 in The d120 and R7041 virus infections induced activation of The d120 and R7041 virus infections induced activation of IRF-3 in U937 cells The U937 cells were infected with ... shown in the present study, that the expression of interferon -induced MxA protein was increased in d120 and R7041-infected cells showing that functional type I IFN was produced These findings...

Ngày tải lên: 20/06/2014, 01:20

... are depicted in Figure 3a The antiviral effect due to interferon alone or in combination is 2-fold increased in cells pretreated with CDM, as an indication that CDM treatment did not interfere with ... current investigation was undertaken to determine if IFN combination inhibited in a synergistic manner HSV-2 replication in Vero cells, and if so to determine if the presence of the plant derived ... cells Interferons Recombinant human Interferon.alpha-2 b (Sidus, Buenos Aires, Argentina) and Interferon-gamma 1B (Boehringer Ingelheim, Ingelheim, Germany) were added to cultures 16 h before infection,...

Ngày tải lên: 20/06/2014, 01:20

... G0/G1 phase into S phase, and induced cells toward apoptosis Next, using similar method, we investigated the effects of p3b/EGFP-N1 on cell cycles in 293 and Vero cells In these cells, the p3b/EGFP-N1 ... apoptosis induction of 3b protein might be a cell type specific In order to further confirm the function of 3b protein in inducing cell apoptosis, a more definitive study using double cell labeling ... signaling by hepatitis B virus-X protein in liver cells Exp Mol Med 2003, 35:301-309 Nakamichi K, Matsumoto Y, Otsuka H: Bovine herpesvirus U(S) ORF8 protein induces apoptosis in infected cells...

Ngày tải lên: 20/06/2014, 04:20

... Our findings of rapamycininduced caspase activation is coincident with Nottingham et al’s [28] result that rapamycin expressed activated caspase in spinal cord of rats Rapamycin-dependent increase ... activities in NNK with rapamycin were significantly lower than those of NNK alone NNK+rapamycin -induced caspase activities were even lower than that of rapamycin control Interestingly, rapamycin induced ... protein level Rapamycin treatment induced significant increase in both Bax and Bid protein expression NNK treatment increased Bax protein expression in time-dependent manner, however, rapamycin...

Ngày tải lên: 07/08/2014, 18:20

... examined TET treatment induced apoptosis and resulted in a decrease in Akt and ERK expression PI3K inhibition had no clear synergistic effect on tetradrine -induced apoptosis, however, ERK inhibition ... 10-14% in the USA [11] Therefore, it is clear that Synergistic effect of ERK inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells Fig The effects of a PI3K/Akt inhibitor ... apoptotic cells was determined by staining cells with annexin V-FITC and propidium iodide (PI) The annexin V-FITC apoptosis detection kit was purchased from Calbiochem (Canada) After incubation, cells...

Ngày tải lên: 07/08/2014, 23:22