salinomycin induced apoptosis in human prostate cancer cells

... human prostate cancer cells a The chemical structure of curcumin and curcumin analog WZ35 b–c The effects of WZ35 or curcumin on cell viability in human prostate cancer cells PC-3 cells or DU145 cells ... cellular apoptosis [10] Because cancer cells have a higher level of oxidative stress than non-malignant cells, in multiple cancer cell types including prostate cancer cells [2] Moreover, curcumin is ... curcumin, exhibited good anti -prostate cancer activity, with an IC50 of 2.2 μM in PC-3 cells However, the underlying mechanism of WZ35 against prostate cancer cells is still unclear Methods: Human

Ngày tải lên: 22/09/2020, 23:26

... 45 induced apoptosis 46 Conclusion: Cell survival and intracellular Ca2+ homeostasis during ER stress in human lung 47 cancer cells are important factors in the induction of the compound K -induced ... pertain ACCEPTED MANUSCRIPT Compound K induced apoptosis via endoplasmic reticulum Ca2+ release through ryanodine receptor in human lung cancer cells Dong-Hyun Shin1, Dong-Gyu Leem1,3, Ji-Sun Shin1, ... Manuscript Compound K induced apoptosis via endoplasmic reticulum Ca ryanodine receptor in human lung cancer cells 2+ release through Dong-Hyun Shin, Dong-Gyu Leem, Ji-Sun Shin, Joo-Il Kim, Kyung-Tack

Ngày tải lên: 19/11/2022, 11:48

... The human ovarian carcinoma cell line SKOV-3, the human breast carcinoma cell line MDA-MB-231, the human glioblastoma cell line U87MG, and the human prostate carcinoma cell line LNCaP were obtained ... prostate cancer models in nude mice Prostate 2000, 42(2):99–106 20 El Etreby MF, Liang Y, Lewis RW: Induction of apoptosis by mifepristone and tamoxifen in human LNCaP prostate cancer cells in ... corresponding increase in p16Ink4A [54] The ability of mifepristone to induce senescence in p16Ink4A-positive prostate cancer cells provides yet another rationale for its potential use as an anti-cancer

Ngày tải lên: 05/11/2020, 07:02

... TRAIL -induced apoptosis showed that the combination of 2-DG and TRAIL enhanced sensitivity of the cells to apoptosis -induced by TRAIL (Figures 1B &1C) The increase in TRAIL -induced apoptosis in ... expressed in human melanoma cells and are induced by the DNA-damaging agent cisplatin Clin Cancer Res 2008, 14:1659-68 Yamaguchi H, Wang HG: CHOP is involved in endoplasmic reticulum stress -induced apoptosis ... mRNA in both cell lines The increase in TRAILR2 mRNA levels induced by 2-DG could be inhibited by pretreatment with actinomycin D (Figure 3E), suggesting that this was due to a transcriptional increase,

Ngày tải lên: 01/11/2022, 08:34

... CD137L -induced apoptosis in SGH-MM6 cells are TNFR-independent SGH-MM6 cells were pre-incubated with TNFR blocking antibody (0.5μg/ml and 1μg/ml for 30 minutes before the cells were cross-linked ... monocytes and increases with increasing monocyte concentrations. CD137-signalling actually induces increase in ROS levels in both monocytes and T cells and by specifically depleting ROS from T cells ... Regulation of protein kinase enzymatic activity in Jurkat T cells during oxidative stress uncoupled from protein tyrosine kinases: role of oxidative changes in protein kinase activation requirements

Ngày tải lên: 11/09/2015, 09:18

... temperature in dark, the cells were washed once with binding buffer and resuspended in 500 µl binding buffer containing propidium iodide solution (PI, 0.5 µg/ml) Annexin V binding and PI infiltration ... signaling pathway for induction of DR5 Indeed, bortezomib -induced DR5 expression is regulated by CHOP, an ER-stress mediator in several cells including human non-small cell lung cancer cells [31] Insignificant ... p53 in human LNCaP-Pro5 prostate cancer cells Cancer Res 2003; 63:7338-7344 Liu J, Qu XJ, Xu L, Zang Y, Qu JL, Hou KZ, Liu YP Bortezomib synergizes TRAIL -induced apoptosis in gastric cancer cells

Ngày tải lên: 15/01/2020, 01:09

... as a downregulation pAkT and β-catenin in vitro in total cell lysates of PC3 cells Since increased β-catenin is apparent in many cancers, including prostate cancer [4, 5, 7], future studies will ... (CREB)-binding protein (CBP) [60], resulting in an increase in the transcription of genes involved in tumorigenesis, angiogenesis, extracellular matrix, and cell cycle progression, including MYC, ... miR-186-5p in metastatic PCa cell models Our findings will aid in the understanding of miR-186-5p’s role in metastatic PCa using pre-clinical, human PCa cell lines Our novel identification of increased

Ngày tải lên: 23/07/2020, 02:34

... MB-PDT in selectively eliminating tumour cells while not affecting normal-like cells Methods Cell cultures Non-tumorigenic human MCF-10A (ATCC CRL-10317™) breast cell line was maintained in phenol ... The incorporation of MB was determined by correcting the absorbance of MB by the number of cells remaining in each well after the incubation period Intracellular singlet oxygen generation Singlet ... therapy induced cell death of hormone insensitive prostate cancer PC-3 cells with autophagic characteristics 2013 Panzarini E, Inguscio V, Dini L Timing the multiple cell death pathways initiated

Ngày tải lên: 19/09/2020, 21:32

... anti-angiogenesis, and apoptosis- inducing activity in various cancer cell lines, including glioma, leukemia, breast, lung, and prostate cancer cell lines [24, 25, 29, 30] by inhibiting NF-kB [29, 31–36] ... specific proteins was carried out with an enhanced chemiluminescence (ECL) assay (GE Healthcare Life Sciences, UK) Sphingosine kinase assay To measure sphingosine kinase activity, sphingosine kinase ... HIF-1α during hypoxia in cancer cells [23] Pristimerin is a naturally occurring triterpenoid quinone methide [24, 25] Several studies have demonstrated that pristimerin is involved in a variety

Ngày tải lên: 20/09/2020, 17:52

... TG2-overexpressing breast cancer cells was found to be hormone-independent in immunocompromised mice Although IL-1β was not applied during in vivo tumor formation, many innate inflammatory cells, including ... performed using SPSS software (SPSS Inc.) Results TG2 overexpression in breast cancer cells results in EMT and stem-cell-like phenotypes To define the signaling pathways involved in TG2dependent ... surface phenotype marker, was increased in MCF7_TG2 cells compared to control cells (Fig 1c) IL-1β induced IL-6 production from breast cancer cells in a TG2-dependent manner In our previous report,

Ngày tải lên: 20/09/2020, 17:56

... activity in both normal breast cells and breast cancer cell lines Results: TPA administration to T47D cells results in a 30 % decrease in cell number at 24 h, which is maintained over 72 h only in ... comparing genes induced by the progestin R5020 in T47D cells with those increased in the luteal-phase normal breast, we have identified a set of genes that predict functional progesterone signaling ... for 24 h Cells were harvested and whole proteins extracted in RIPA buffer (Pierce, Rockford, IL, USA) including protease inhibitor cocktail and EDTA Protein concentration was determined using the

Ngày tải lên: 21/09/2020, 01:25

... development [18] and in skeletal muscle adaptation [19] ADAM19 contains several domains, including a prodomain, metalloproteinase domain, disintegrin domain, cysteine-rich domain, epidermal growth ... emerging evidence of ADAM involvement in human cancer, we were interested to investigate if ADAM19 might play a role in prostate cancer using a combination of clinical cohorts and in vitro analyses We ... metalloproteinase domain of ADAM19 is the catalytically-mediated ectodomain shedding of substrates [15] The disintegrin domain of ADAM19 functions as an adhesion domain by binding to integrins α4β1

Ngày tải lên: 21/09/2020, 02:00

... autophagy and apoptosis induction Results: In SiHa cells, there was an inverse correlation between miR-21 expression and PTEN mRNA level as well as PTEN protein expression in cervical cancer cells Transfection ... Biotinilated and pre-stained molecular weight marker was included IgG mouse monoclonal antibody sc-7974-HRP was used to detect human PTEN protein Human beta-actin protein was detected using IgG ... to induce selective and specific silencing of miR-21 microRNA in human cervical cancer cells infected with HPV16 siRNA-mediated silencing of miR-21 expression has an effect on PTEN expression In

Ngày tải lên: 21/09/2020, 09:20

... reduced in metastatic cells relative to the primary tumor corroborating the cell line findings [36] Following RNAi-mediated knockdown of HP1α, poorly invasive MCF7 cells have increased invasive ... expression in invasive versus poorly invasive breast cancer cells [40] Also, CBX5 promoter binding of the transcription factor YY1 is involved in regulating the differential expression levels in breast ... distributions with HP1α present mainly in heterochromatin, HP1β in both hetero- and euchromatin, and HP1γ primarily located in euchromatin [5, 8, 9] Tethering HP1 proteins to chromatin through the CD, CSD

Ngày tải lên: 21/09/2020, 11:12

... signaling pathway in human TCC cells To better understand the mechanisms of AD198 and Dox -induced cell growth inhibition in T24 and UMUC3 cells, we investigated role of PI3K/AKT and MAPK signaling ... effect in generating ROS in murine cardiomyocytes [23] However; ROS production was induced by AD198 more than by Dox in both tested human bladder cell lines (Fig 2) In addition to ROS production in ... normalized to actin and then to control group as shown in Fig 3c A statistically significant increases in Fig Dox and AD198 induced ROS in human TCC cells (a) T24 and (b) UMUC3 cells were treated

Ngày tải lên: 22/09/2020, 22:51

... intrinsic pathway during MFX and CFX induced apoptosis via Bid Fluoroquinolones induced apoptosis is caspase-8 dependent In order to confirm the role of caspase-8 in FQ induced apoptosis we first ... induces apoptosis in pancreatic cancer cell lines We further observed CFX to be more potent than MFX in inhibiting proliferation and induction of apoptosis in both the cell lines A decrease in ... 0.006) treatment in both the cell lines (Fig 5) To rule out the involvement of p53 in FQ -induced apoptosis we simultaneously performed annexin assay in HCT116 (human colon cancer cell line) wild type

Ngày tải lên: 28/09/2020, 10:18

... prolactin in human breast cancer cells Katja Linher-Melville and Gurmit Singh* Abstract Background: Liver kinase (LKB1) is an important multi-tasking protein linked with metabolic signaling, also ... Chinese hamster ovary (CHO-K1) cells (ATCC #CCL-61) were cultured in DMEM/F12 containing 5% FBS and penicillin/streptomycin T47D cells were Page of 19 maintained in RPMI-1640 with 10% FBS, in ... All human cell lines were used in accordance with institutional biosafety guidelines MDA-MB-231 human breast cancer cells at low passage (less than 20 passages, ATCC #HTB-26) were maintained in

Ngày tải lên: 14/10/2020, 17:09

... E2- but not EGF -induced proliferation To determine whether EGF -induced EGFR signalling resulting in tamoxifen resistance involves ERα or not, we introduced a siRNA targeting ERα in both parental ... Santen RJ: Estrogen signaling via a linear pathway involving insulin-like growth factor I receptor, matrix activated protein kinase in MCF-7 breast cancer cells Endocrinology 2007, 148(8):4091–4101 ... signalling in this process, using estrogen responsive MCF7 cells that have increased expression of wild type EGFR We showed that EGF-driven signalling in these cells is sufficient to maintain ERα-independent

Ngày tải lên: 05/11/2020, 00:10

... GTP-binding protein rab22b Minichromosome maintenance protein homolog Ribosomal L1 domain containing Hepatocellular carcinoma-associated protein HCA10 Chromosome 20 ORF 30 Splicing factor proline ... that prevents the apoptosis induced by cisplatin and doxorubicin [29,30] In addition, irinotecan -induced changes in MT expression correlated with clinical response in gastric cancer patients, ... protein), c11 Polyglutamine-binding protein FK506-binding protein Phosphoserine phosphatase-like Chromosome 15 ORF 24 Coiled-coil domain-containing protein 72 Dihydropyrimidinase-like ADP-ribosylation...

Ngày tải lên: 06/03/2014, 22:21

... phosphorylation of Bcl-2 following a variety of stimuli These include paclitaxel-activated Raf-1 [15], paclitexel- or vincristine -induced protein kinase A [16], bryostatin- 1induced mitochondrial localized ... Results To examine the kinetics of the apoptotic cell death induced by ionizing radiation in human cervical cancer cells, we treated HeLa cells with 10 Gy radiation, and analyzed induction of apoptotic ... radiation -induced mitochondrial cell death M.-J Kim et al Fig Ionizing radiation induces expression of Fas and activation of caspases in human cervical cancer cells (A) Ionizing radiation-induced...

Ngày tải lên: 07/03/2014, 05:20