instrumentation for cell mechanics
Force controlled biomanipulation for biological cell mechanics studies
Ngày tải lên :
10/09/2015, 15:47
... actuator for force generation, a micro-indenter equipped with a piezoresistive micro-force sensor for applying a prescribed force on the cellular surface and a compound flexure stage for transmitting ... equipped with a piezoresistive microforce sensor for applying a prescribed force on the cellular surface, and a compound flexure stage for transmitting the force from the voice coil actuator to ... osteoarthritis [21] For example, in cytokinesis (cell division process), successful cell division has been regarded as critical to human health Any external force applied to cells must be properly...
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Practical instrumentation for automation and process control 2
Ngày tải lên :
01/01/2014, 21:15
... Analytical Instrumentation for Engineers and Technicians Practical Flow Measurement for Engineers and Technicians Practical Intrinsic Safety for Engineers and Technicians Practical Safety Instrumentation ... Practical Boiler Control and Instrumentation for Engineers and Technicians Practical Process Instrumentation for Engineers and Technicians Practical Motion Control for Engineers and Technicians ... Shut-down Systems for Industry Practical Process Control for Engineers and Technicians Practical Programming for Industrial Control – using (IEC 1131-3;OPC) Practical SCADA Systems for Industry Practical...
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Báo cáo khoa học: A mammalian monothiol glutaredoxin, Grx3, is critical for cell cycle progression during embryogenesis doc
Ngày tải lên :
14/03/2014, 23:20
... (f) staining cells Scale bars = 200 lm (F) Quantification of binucleated cells in control and Grx3-KD cells Total 600 cells counted for control cells and 360 cells counted for Grx3-KD cells The bars ... to finish cell cycle back to G1 phase Cells were harvested at 2-h intervals Half of cells were stained with propidium iodide, and analyzed by FACS Another half of cells were prepared for cell lysate ... °C for The cells were washed three times with NaCl ⁄ Pi for each, and then blocked in 10% normal goat serum for h at room temperature The cells were incubated in mAb against b-actin (1 : 100) for...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
Ngày tải lên :
16/03/2014, 18:20
... chosen for hCypA labelling were 50 lM for [15N]Trp and [15N]Tyr, 150 lM for [15N]Ile, [15N]Thr and [15N]His, 0.35 mM for remaining Group II [15N]amino acids, and mM for those in Group III The S30 cell ... Value for Saccharomyces carlsbergensis, no data available for E coli; b value for Lupinus luteus and bovine, no data available for E coli; c value for Paracoccus denitrificans, no data available for ... evidence for more than a single conformation of His70, neither at pH 7.5 nor at pH 6.5 Discussion In this study, several aspects of a cell- free protein synthesis system based on an E coli cell extract...
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instrumentation for process measurement and control, third editon
Ngày tải lên :
01/04/2014, 11:03
... electrical signal for an electronic controller or a pneumatic signal for a pneumatic controller Measuring transmitters bave attained great popularity in the process industries They perform the measurementand ... outputs For instance, the output of analog transmitters is commonly to 20 mA dc Symbols A set of symbols has beenadopted to show instrumentation layouts and to make these layouts more uniformo ... manipuINSTRUMENT FOR SINGLE MEASURED INSTRUMENT INSTRUMENT MOUNTED ON BOARD LOCALLY MOUNTED VARIABLE INSTRUMENT MOUNTED BEHIND BOARD NSTRUMENT FOR TWO MEASURED Fig 1-7 Instrumentfor measured variables...
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sonntag, borgnakke, van wylen. solution manual chapters 1-9 for classical mechanics and thermodynamics
Ngày tải lên :
24/04/2014, 16:54
... motion for the mass m2 along the downwards direction, in that case the mass m moves up (i.e has -a for the acceleration) m2 a = m2 g − m1 g − m1a (m1 + m2 ) a = (m2 − m1 )g This is net force in ... up, spilling the water over the edge Deduce the formula for the air pressure as a function of the piston elevation from the bottom, h 2.28 Solution: Force balance Piston: F↑ = F↓ P0 H h 2.29 P PA ... single point force of 250 N presses down on piston A Find the needed extra force on piston B so that none of the pistons have to move Solution: No motion in connecting pipe: PA = PB & Forces on...
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Applied technology and instrumentation for process control
Ngày tải lên :
15/05/2014, 11:03
... module X before it forms the measurement input to the airflow controller The oxygen controller adjusts the ratio for the amount of combustion air required for Applicability of miscellaneous control ... and former colleague for his forbearance, patience, long hours of work, and invaluable comments in his review of this text My patient wife Halina, also, once again deserves special mention for ... a gas using four separate cells of equal resistance value arranged in the form of a Wheatstone bridge Two of the cells are open to the gas being measured (measuring cells), while the other two...
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Báo cáo hóa học: "Research Article Novel Heuristics for Cell Radius Determination in WCDMA Systems and Their Application to Strategic Planning Studies" potx
Ngày tải lên :
21/06/2014, 23:20
... to be done specifically for each one, and of course for the uplink and the downlink Therefore the formulation explained previously, and the value R p , has to be applied for each service i and ... has to be done also for the uplink direction (UL) Therefore, at the end we have obtained another set of two vectors (one for the uplink and one for the downlink), with the cell radius by capacity ... LTotal i are LTotal = 0.105 for the service S1 , voice, LTotal i = 0.271 for S2 , data 64 Kbps and LTotal i = 0.373 for S3 , data 144 Kbps The results for the downlink cell radius per traffic are...
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Aseptic Technique for Cell Culture pptx
Ngày tải lên :
11/07/2014, 21:20
... Technique for Cell Culture 1.3.2 Current Protocols in Cell Biology Prepare and maintain the work area Perform all aseptic work in a clean work space, free from contaminating air currents and drafts For ... needed Cell Culture 1.3.7 Current Protocols in Cell Biology for aseptic work Laminar-flow cabinets create clean working environments (see below); clean, certified cell lines are available from cell ... of steps Aseptic Technique for Cell Culture Laminar-flow cabinets Laminar-flow cabinets or hoods have replaced the open laboratory bench for aseptic work in almost all cell culture and microbiology...
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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
Ngày tải lên :
09/08/2014, 01:23
... cytometry analysis of cells Either isolated cells from OC were directly used for flow cytometric analysis or cells were used after adherence and cultivation, as described above Cells were washed ... Germany) Dead cells were excluded by propidium iodide (Sigma) staining Cells were gated on forward and side scatter to exclude debris and cell aggregates To calculate the percentages of cells staining ... Fluorescence-activated cell sorting For cell sorting, native isolated cells from OC were stained with saturating concentrations of CD9-FITC, CD90-allophycocyanine and CD166-PE Single cells were sorted...
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Báo cáo y học: "Intervertebral disc cells as competent phagocytes in vitro: implications for cell death in disc degeneration" pptx
Ngày tải lên :
09/08/2014, 10:23
... at 37°C The cells were expanded and passaged twice before use Two cell lines, THP-1 and J774 cells, were used as committed phagocytes whilst the fibroblast cell line, L929, was used for comparison ... apoptotic cells by nucleus pulposus cells NP cells were fluorescently tagged red with a cell linker kit (Sigma-Aldrich) and allowed to adhere overnight NP cells (1 × 105) in mL/well were used HeLa cells ... assessed by recording at least 200 cells for each cell culture at each time point Results Morphology of apoptotic disc cells Hoechst 33342-stained nucleus cells pulposus cells demonstrate typical apoptotic...
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báo cáo khoa học: "Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture" potx
Ngày tải lên :
11/08/2014, 00:22
... cell/ Ntot cell × 100, (4) where Npolyg cell is the number of the cells showing the polygonal-like morphology and Ntot cell is the total number of counted cells in each image For the cell adhesion ... streptomycin Cells were grown in tissue culture flasks at 37°C in controlled atmosphere (5% CO2) For cell adhesion and proliferation MDCK cells were seeded at a concentration of 104 cells/well ... = [1 - (polygonal-like cell (%)/polygonal-like (5) cell (%)contr] × 100, where polygonal-like cell (%) as defined in Expression and polygonal-like cell (%)contr is the cell adhesion on each substrate...
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báo cáo khoa học: "Nanofibers and nanoparticles from the insectcapturing adhesive of the Sundew (Drosera) for cell attachment" ppsx
Ngày tải lên :
11/08/2014, 00:22
... attached cells per mm2 yielded 147 cells/ mm2 for the negative control, 1681 cells/mm for the positive control, and 1253 cells/mm for the Sundew adhesive coated surface Due to the non-uniformity ... cells were chosen as a model system for nerve cell growth PC12 cells were derived from a pheochromocytoma of the rat adrenal medulla [31], and are typically used as a model system for nerve cell ... 2.5% fetal bovine serum at a density of × 10 cells/cm The cells were then incubated on the samples for 24 hours in a 37°C incubator with 5% CO2 to allow for attachment After 24 hours, the samples...
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Báo cáo y học: " Analysis of HIV-1 Vpr determinants responsible for cell growth arrest in Saccharomyces cerevisiae" ppt
Ngày tải lên :
13/08/2014, 13:20
... GST-B4 suppression of Vpr-induced cell growth arrest Yeast co-transformants were grown in non-inducible selective medium for two days Similar number of yeast cells were then serially diluted, ... of Vpr are crucial for GST-B4 binding, whereas the C-terminal domain is dispensable for the interaction Vpr mutants defective for GST-B4 binding are unable to arrest yeast cell growth We next ... selective medium (Trp- or Trp-/Ura-, 2% raffinose (raf+)) for days Then, suspensions of transformed HP-16 yeast cells (adjusted at similar cell densities) were serially diluted and spotted onto...
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Báo cáo y học: "An ontology for cell types" potx
Ngày tải lên :
14/08/2014, 14:21
... histology (for example, epthelial _cell, mesenchyme _cell) , lineage (for example, ectodermal _cell, endodermal _cell) and ploidy (for example, haploid _cell, polyploid _cell) The present version of the Cell ... colleagues for their help in developing this resource, in particular to David States for information on vertebrate blood cells, David Hall for information about C elegans, Rex Chisholm for information ... of the Cell Ontology are cell_ in_vivo and experimentally_modified _cell The former includes cell types that occur in nature, the latter those that are experimentally derived, including cell lines...
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Engineering scaffold and soluble cues for cell instruction
Ngày tải lên :
09/09/2015, 10:19
... harvesting of cells from a donor These cells are usually expanded in vitro to generate more cells or differentiated through exposure to specific cytokines to yield desired cell types These cells are ... engineering Cells are harvested from donor and expanded in vitro to generate more cells These cells can then be combined with scaffolds and growth factors to form engineered tissues for implantation ... scaffold mechanics in regulating cell signaling and cell cycle.[6] Apart from use in tissue engineering and regeneration applications, these scaffolds are also increasingly used for both cell culture...
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Bio functionalization of electrospun nanofibre scaffolds for cell culture applications
Ngày tải lên :
12/09/2015, 09:08
... as cell culture scaffolds that can promote cell- substrate interactions and are bioactive in soliciting favorable cellular responses like cell adhesion, cell morphological reorganization, cell ... Summary bioactive in regulating cellular responses like cell adhesion, cell morphological reorganization, cell differentiated functions, cell proliferation or cell phenotype maintenance (3) Demonstrated ... a niche for cells to proliferate and differentiate The intended uses for scaffold-based cell cultures are vast: In some applications, the cells develop into tissues that are suitable for implantation,...
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Development of dielectrophoresis devices for cell manipulation
Ngày tải lên :
14/09/2015, 11:45
... separation that can be used for separation of bacteria, yeast and plant cells The method uses castellated electrodes for cell trapping, two ports for outlet, and one for inlet Particles flow from ... and to perform cell fusion at the field constriction (Masuda et al., 1989) 2.3.2.2 Levitation Levitation relies on two opposing forces, the negative DEP force and the net gravitational force They ... Re [K] for viable and non-viable yeast cells in a suspending medium with a conductivity of mS/m………………………….…… 94 3.52 Variation of hydrodynamic force and positive dielectrophoretic force for differentelectrode...
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Fabrication of proton beam micromachined scaffolds for cell behaviour studies
Ngày tải lên :
06/10/2015, 20:42
... MICROMACHINED SCAFFOLDS FOR CELL BEHAVIOUR STUDIES ZHANG FANG (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF PHYSICS NATIONAL UNIVERSITY OF SINGAPORE 2004 for mum, dad and ... grateful for the DVD man, Andrew for offering me the micromachining software and the figure of hardware I thank him and Ee Jin for providing happy memory in my life in S’pore Don’t forget to ... grateful for Frederic, Mukhtar and Huang Long for always treating me like a gentleman I also acknowledge Reshmi for encouraging me to turn my dreams into reality Special thanks go to Soma for taking...
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