indian tribes involved in the trail of tears
Risks Involved in the Use of Herbal Products
... exists in the antioxidant and otherbioactivities of flavonoids, which are powerful in assays conducted in vitro; the 14 Risks Involved in the Use of Herbal Products 353commonly found in over -the- counter ... to inhibit phase I and induce phase II carcinogen metabolizing enzymesthat initiate carcinogenesis. They also inhibit the promotion stage of carcinogen-esis by inhibiting oxygen radical-forming ... consistent in classifying the antioxidant capacity of the polyphenol-rich beverages in descending order: Punica granatum L. (pomegranate)14 Risks Involved in the Use of Herbal Products 361ephedrine...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... involving the C-terminal part of the polypeptide chain (Fig. 7). In the latter, differences in the conformation of the loop betweenFig. 6. Stereo picture of the model of the a-carbanion/enamine intermediate ... presence of DNA-bindingproteins involved in regulation of the gene expression of indole-3-pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acidbiosynthesis in Azospirillum lipoferum ... model of the a-carbanion/enamine intermediate of the substrate indole-3-pyruvate with ThDP in the active site of IPDC was built based on the three-dimensional structure of the corresponding intermediate...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... proteinsimplicated in the biosynthesis of the aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... be implicated in the biosynthesis of the aminonucleoside moiety of puromycin[6–8]. Interestingly, their organization is identical to that of the pur cluster, with the exception of pur6, which ... shown)[33,34]. In contrast, its similarity to type III PKSs, whichlack this domain, is scant. This domain promotes the binding of the acyl-CoA initiation unit to the ketosynthetasedomain of the PKSs...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
... synthesis and we can onlystate that the latter certainly does not exceed the rate of amylopectin synthesis. The increase in crystallinity wit-nessed during these first 12 h is in line with the ... from the results listed in Table 1 that most, if not all, of the amylose issynthesized in vivo when the rate of amylopectin synthesisbecomes minimal. At these moments the rates of amylopec-tin ... at the final stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]). In vitrosynthesis of amyloseBecause in Chlamydomonas amylose synthesis remainsactive...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
... catalytic site of GalNAc-T1, might be involved in the catalytic function of the enzyme.To investigate whether the cysteine residues modified byPCMPS are involved in the binding of UDP-GalNAc,we ... cysteine residues not involved in the formation of a disulfide bond), by cysteine-specific labeling, to study the mechanistic involvement of the conserved cysteine residues in the function of GalNAc-T1. ... amajor site of interaction with UDP-GalNAc, Cys214 is also involved in binding.PCMPS and DFP modification of the serine-mutants of GalNAc-T1We also examined the sensitivity of the three serine mutantsto...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
... degree of protection by ADP sug-gests that the mitochondrial injury is caused by open-ing of the mPTP. There is evidence that ADP inhibitsopening of the mPTP by occupying binding siteslocated in ... reperfusion in brain.There is a growing body of information concerning the mechanism of ROS generation by the respiratorychain in mitochondria. Complex I and complex IIIwere identified as generators of ... –Ca2+,15 min in incubation medium; Ca2+, 15 min in incubation mediumplus 3.5 lM Ca2+;H⁄ R, 10 min hypoxia followed by 5 min reoxy-genation; CSA, 2 lM cyclosporin A. A volume of 2 mL of the incu-bation...
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Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... of XDH requires the incorporation of the MPT center, placement of the terminal sulfur ligand on the Mo, and the incorporation of two [2Fe)2S] centers and anFAD into the recombinant enzyme. Therefore, ... of the level of culture aerationon the expression of recombinant protein. Raising the cellular redox potential by increasing aeration levels isexpected to reduce the level of cysteine and increase ... in R. cap-sulatus. The protein product of xdhC appears to exhibit aredox role in maturation of the XDHABCby eliminating the dependence of its functional expression on the redox state of the...
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Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf
... only one, the atpF intron, while CRS2 is involved in the splicing of nine of the 10 chloroplast group IIB introns [6,15,37]. The expression of CRS2 in E. coli together with the corresponding RNA ... in the UV-crosslinkingcompetitive assays, as shown in D. The IC50 of each RNA wasdetermined by plotting the data (D). The location of the high affinitybinding site in the Dd120 RNA defined in ... splicing products of the petB intron could be that the protein binds to the UTR region between psbH and petB, and to domain IV of the petB intron. The binding of the homodimer of HCF152 in this region...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
... confirmed the results obtained in the pull-down assays, showing that there is a physicalinteraction between the N- and C-terminal domains of SSIII in vitro.Mapping of the CD-binding region in the N-terminal ... D3 domains,respectively, but not the individual starch-binding domains, are involved in the interaction with the catalytic domain. We also determined that the resi-dues W366 and Y394 in the D2 ... acids of the N-terminalregion responsible for SSIII regulation. We foundevidence indicating that two regions, D(316–344) in the D2 domain and D(495–535) in the D3 domain, are involved in the interaction...
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Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
... subcellular compartments other than lipid droplets. The expression of CIDEC is elevated during the differentiation of adipocytesTo gain further insight into the roles of CIDEC in adipocyte differentiation, ... analyses of the relativelevels of the indicated proteins after wes-tern blotting (as in D) were carried out. Simi-lar experiments were performed five timesand the intensity of the individual ... of interest in the study of adipocyte biology [1]. Owing to an imbalancebetween energy intake and expenditure, obesity is oftencharacterized by an increase in both the size and the number of...
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Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc
... ⁄ ))]. These results indicatethat a functional deletion of Synoviolin causes a change in the intracellular trafficking of NCT.Effect of Synoviolin on the production of AbNCT is one of the essential ... Synovi-lin-mediated ubiquitination also regulates the traffick-ing of NCT within the Golgi compartment, because the maturation of the sugar modification of the pro-tein occurs within the Golgi ... (2008)Novel role of presenilins in maturation and transport of integrin b1. Biochemistry 47, 3370–3378.T. Maeda et al. Synoviolin is involved in the degradation of nicastrinFEBS Journal 276...
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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx
... sequences of the catalytic domain of members of the GH18 familyresulted in the amplification of an expected 147 bpsequence. Cloning and sequencing of this fragmentrevealed an ORF showing amino acid ... of the soleGlyco_18 domain, the C-terminal tail of C. gigas CLPsmay not noticeably contribute to the structure and the function of these proteins. Interestingly, Cg-Clp1 andCg-Clp2 C-terminal ... by incubating sections in 0.3% hydrogen peroxide in methanol for 30 min at room temperature. Slides werethen washed and incubated in a blocking solution accord-ing to the manufacturer’s instructions....
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Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf
... further increased. The unfolding curveusing the ANS fluorescence probe suggested a multistateprocess, consistent with the results obtained by proteinintrinsic fluorescence and CD. The binding ... compared to the calculated molecular weight of monomeric d2-crystallin.Multistep unfolding of monomeric d2-crystallinFurther increases in GdnHCl concentration induced unfold-ing of the dissociated ... accuratelydescribes the dissociation/unfolding process of d2-crystallin.Another possible origin of the discrepancy between the results of Sampaleanu et al. [30] may be the denaturationtime used in these...
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