in situ hybridization at the electron microscopic level

... some of the specific features naturally occurring in the language used during the Task Cycle Learners examine the language forms in the text and look in detail at the use and the meaning of lexical ... educators in the world as the purpose of learning a second language is to communicate with each other in society This is a reason why the Ministry of Education and Training needs to innovate the ... comprehending, manipulating, producing or interacting in the target language while their attention is principally focused on meaning rather than form" (p.10) He suggests that in all definitions of tasks,...

Ngày tải lên: 07/09/2013, 13:02

... planning groups & Pre-planning groups)  Records: All the group discussions and the individual presentations from No planning group & Pre-planning group Group Data Analysis Language Related Episodes ... Suggestions  Group planning prior to individual presentations is really useful  Group planning should be applied and encouraged in the classroom  Further study: - Investigate in three areas: fluency, ... group work - Relationship between group work and individual presentation Objectives of the study  contribute more theory to the understandings of group discussion  find out the essentials of...

Ngày tải lên: 29/01/2014, 10:33

... than the precision The CCQ scores of the patients were within the limits of the MCID of the scores of the treating clinician in 62% and the mean score of the reviewing clinicians in 63% The proportion ... about the mental state domain The experience in measuring health status and the change in history taking might contribute to the high agreement between the scores of the patient and treating clinician ... clinicians participated in the recruitment of the patients and the scoring of the CCQ, as the main research question was the patient-reviewer agreement These two clinicians previously used the...

Ngày tải lên: 20/06/2014, 15:20

... Sensitive in situ hybridization technique using biotin – streptavidin – polyalkaline phosphatase complex J Clin Pathol 40: 163 – 166 26 Kirsten M Spann, Russell J McCulloch, Jeff A Cowley, Iain J ... M Redman, James A Brock, 1998 The geographic distribution of Taura Syndrome Virus (TSV) in Americas: determination by histopathology and in situ hybridization using TSV – specific cDNA probes ... Procedures Online 3: – Japan 18 Baumgart E., Schad A., Grabenbauer M., 2001 In situ hybridization: general principles and application digoxigenin – labeled cRNA for detection of mRNAs In Immunocytochemistry...

Ngày tải lên: 28/07/2014, 02:21

... mẫu paraffin Đặt mẫu xử lý vào khuôn inox, dùng bình rót paraffin (5 paraffin : sáp ong) vào khuôn chứa mẫu, cố định mẫu cho nằm yên dƣới đáy tách rời Sau đặt khuôn inox chứa mẫu paraffin lên bề ... gồm: Probe đánh dấu DIG (Digoxigenin) SSC 4X (Sodium Chloride/Sodium Citrate) buffer Formamide 50% Denhardt’s (Bovine Serum Albumin, Ficoll 400, PVP) Dextran Sulfate 5% DNA cá hồi 0,5 mg/l Thành ... Paraffin (1) Paraffin (2) giờ Sơ đồ 3.1: Quy trình xử lý mẫu trƣớc thực chẩn đoán Tổng thời gian xử lý mẫu: 12,5 Paraffin đƣợc dùng quy trình xử lý mẫu hỗn hợp với sáp ong theo tỷ lệ paraffin :...

Ngày tải lên: 28/07/2014, 02:21

... : In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... 14 II.4.2.5 Phòng trị bệnh 14 II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION 14 II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization 14 II.5.2 Khái niệm lai phân tử 15 ... trắng II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization Phƣơng pháp In Situ hybridization (ISH) đƣợc phát vào năm 1969 công Gall Pardue Đây phƣơng pháp lai...

Ngày tải lên: 28/07/2014, 02:21

... 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization of porcine epidemic diarrhea virus in the intestinal tissues from naturally infected piglets Vet Pathol 2000, 37, 62-67 ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled ... of hybridization, sections were incubated with anti-digoxigenin conjugated with alkaline phosphatase (Roche) for digoxigenin-labeled probe and streptavidin conjugated with alkaline phosphatase...

Ngày tải lên: 07/08/2014, 17:22

... to the distribution of endogenous β-actin mRNA at the time of fixation of the cell Our ISH results also showed that dark staining was observed around the nucleus predominately, indicating that ... background intensity In is the darkness intensity of each point of the cell In this equation, we considered (In/ I0) as the transmission Since the background intensity was stable, we calibrated the darkness ... mainly in the vicinity of the nucleus, and these results agreed with the results of our previous work and other studies by ISH [13,20] Alkaline phosphatase activity (dark intracellular staining)...

Ngày tải lên: 11/08/2014, 00:22

... (Gellin et al., 1985), and we added only some modifications concerning the washings after hybridization: the slides were first rinsed in x SSC at room temperature, and in 50% formamide and x SSC at ... results The high specific activity could partly explain the efficiency of in situ hybridization This observation confirms the one given by Lin et al 1985 We have also modified the washings after hybridization ... to locate the silver grains and identify the chromosomes The chromosomes were classified according to the G-band pattern, defined by the Committee for the standardized karyotype of the Domestic...

Ngày tải lên: 14/08/2014, 19:23

... DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific in situ hybridization ... pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland, E.B., 1992 Development of a rapid method for detecting bacterial cells in situ ... hybridization of the intracellular organism of porcine proliferative enteropathy Vet Pathol 31, 462–467 [48] Gersdorf, H., 1993a Identification of Bacteroides forsythus in subgingival plaque from patients...

Ngày tải lên: 30/10/2012, 11:02

... of the role of the learners in ESP teaching Based on the theory of learner-based teaching, we know that class activities can be done using information that the students themselves bring to the ... for their good points, correct large-scale errors, and explicit some confusing matters Suggested ways for improving the peer teaching process In light of the initial findings, the investigator introduces ... sure that the students understood what was written or what was asked in the questionnaire, the investigator decided to translate the questionnaire for the students into Vietnamese In the end,...

Ngày tải lên: 29/01/2014, 10:49

... address the following questions: (a) can coupled fumarate respiration be restored by incorporating the isolated enzymes into liposomes containing menaquinone; (b) is the Dp generated by menaquinone ... by the addition of Triton X-100 [11,19,30] (not shown) There was no stimulation by Triton X-100 in the preparations obtained at the critical or lower ratios, indicating that all the fumarate ... derivatives of pHydcat [1] Plasmid pHydcat contains the entire hydABC operon and integrates into the genome of W succinogenes by homologous recombination Derivatives of pHydcat were synthesized...

Ngày tải lên: 21/02/2014, 03:20

... Turning to Chart 1b, the maturity of the instruments also plays prima facie a role in determining the level of bank lending rates: the lower the maturity the higher the level of the bank lending ... into the determination of retail bank interest rates in the euro area The main contribution of this study is that for the first time both bank deposit rates and lending rates at the level of the ... variables in levels and in first differences (the change in the interest rate) The market interest rates analysed, with Reuters as data source, are the overnight market interest rate, money market rates...

Ngày tải lên: 15/03/2014, 14:20

... writing skill at the class IX level and then to apply picture story writing as an innovative teaching strategy to find out if there is expected improvement in their writing performance or otherwise ... chapter begins by discussing the importance of English in global context, followed by the importance and status of English in India The ELT situation at the school level in rural India is briefly ... Teaching Writing Using Picture Stories as Tools at the High School Level: The Movement from Other Regulation to Self-Regulation – M.Phil Dissertation 22 1.3 The ELT Situation in Rural India...

Ngày tải lên: 24/03/2014, 19:20

... formamide/2× SSC in a plastic Coplin jar Heat in a water bath to 70°C Preheat the slides to about 70°C, in an empty plastic Coplin jar Denature the DNA on the slides by immersing them in the denaturation ... Place in a water bath at 70°C Check the temperature inside the jar 2a Prewarm slides at 60°C in dry heat oven 3a Immerse the slides in the denaturation solution for exactly min, two slides at a ... bath and bring the temperature to 70°C Meanwhile, heat the slides at 50°C to avoid lowering the temperature of the denaturing solution Immerse one to four warmed slides in the denaturation solution...

Ngày tải lên: 11/04/2014, 09:47

... Low-stringency post -hybridization wash done at 4°C in SSC with 2% BSA for min, followed by incubation with anti-DIG/alkaline phosphate conjugate antibodies (Enzo Diagnostics, NY) in a heater at 37°C ... making the TMAs RB and LTB supervised and participated in the study design, result interpretation and in the writing All authors read and approved the final manuscript Competing interests The authors ... formalin-fixed tissue, making them ideal candidates for investigation in routinely processed material Among the miRNAs, miR-155 is particularly interesting as it is consistently involved in several neoplastic...

Ngày tải lên: 18/06/2014, 16:20

... hydrolyze the organotitanium at a controlled rate (Scheme 1) We have called this a double in situ approach, because the in situ synthesis of the nanoparticle (TiO2) coincides with the in situ polymerization, ... is water The presence of the two layers indicates that the hydrolysis reaction has occurred as predicted, while the quantities of water and BuOH indicate the extent of each reaction The theoretical ... burning drips While the increase in melt viscosity is to be expected on incorporation of well-dispersed nanofiller, this stabilization of the polymer matrix allows the surface temperature to increase...

Ngày tải lên: 22/06/2014, 01:20

... in the replication rate when the force exceeds pN The decrease in the replication rate is attributed to the work that DNAP has to perform against the external load to contract the template in ... modifications in extremities to provide binding sites for avidin and microsphere occur at one strand only, leaving the other 5¢ terminus exposed to initiate the digestion As in the Fig DNA stretching ... digestion rate exhibits large fluctuations depending on the position along the DNA template, suggesting that a sequence-dependent melting of the base pair (bp) is the rate-limiting step in the enzymatic...

Ngày tải lên: 22/06/2014, 18:20

Ngày tải lên: 28/06/2014, 15:23

... transfer by industries in the Province Secondly, education and training system (including vocational training) should be coherent with development planning of industries in the modernization and industrialization ... labor with intermediate level training greatly increased, from 31,8% to 45,39% in the same period (increase of 13,6%) The percentage of people at collegial training level had the highest increase ... laborers at under intermediatelevel training held high percentage, in 2001, almost 78% , in 2005, almost 76,5% in 2010, 69,5% , in 2011, 68,3% For laborers with intermediate -level training, in 20012005,...

Ngày tải lên: 14/07/2014, 13:33