in silico prediction and characterization

... corresponding to 350 mM NaCl and dialyzed against buffer A Characterization of the recombinant laccase Protein analysis Protein concentration was determined according to Lowry et al [23] with bovine ... transferred in standard reaction mixtures to determine the laccase activity with ABTS and syringaldazine Mycelial dry weight and pH (g.L −1) P cinnabarinus laccase gene expression in A niger (Eur ... from the dikaryotic strain I-937 of P cinnabarinus and 145 mgÆL)1 from the derived monokaryotic strain ss3 of P cinnabarinus In order to improve the secretion of the recombinant laccase, the laccase...

Ngày tải lên: 17/03/2014, 11:20

... variants identified in rounds and 3, and 20 of the 38 identified in round 4) In every case, we observed a frameshift caused by insertion of a single thymine nucleotide into the fourth randomized codon ... (b1) and Gln332 (b4), remain in trPRAI It is tempting to speculate that, in the absence of the salt bridge linking Lys258 and Asp425 (b8), the hydrogen bond donor Lys258 could instead be involved ... amino-terminal autonomous folding unit controls several rate-limiting steps in the folding of a single domain protein Biochemistry 38, 10205–10214 ´ ´ 45 Soberon X, Fuentes-Gallego P & Saab-Rincon...

Ngày tải lên: 30/03/2014, 20:20

... binders) and C (4-wt.% YF3 + 3-wt.% CaF2 and 4.5-wt.% organic binders) sintered at 1750ºC for 2h after de-waxing in Air 228 Advances in Ceramics - Synthesis and Characterization, Processing and ... metal and ceramic parts called Direct Metal Laser Sintering (DMLS), the Laminated Object Manufacturing (LOM) process, Fused Deposition Modelling (FDM), 3D Printing (3DP) and the Inkjet Printing ... showing that the additive chemistry and the doping level both play crucial roles in determining the oxidation behaviour of fully sintered AlN Using different amounts and proportions of sintering...

Ngày tải lên: 29/06/2014, 13:20

... aggressive media, and various kinds of wear These are, first and foremost, cutting and stamping tools; forming rolls; parts in aviation engines, gas turbines, and compressors; slider bearings; nozzles ... by determining the dihedral surface angle of defined compacts sintered in solid-phase under certain conditions The surface modification and properties induced by a laser beam in pressings of ceramic ... austenite and discrete carbide particles The problems arising in certain applications of sintering, which consists the main operation in powder technology, can be identified and described using modern...

Ngày tải lên: 29/06/2014, 13:20

... signaling molecules in the initiation and execution of apoptosis, as well as in other important cellular processes such as inflammation and differentiation In an attempt to further the understanding ... in apoptosis: intrinsic and extrinsic (Figure 1-3) The extrinsic pathway is initiated when the CD95 ligand binds to the CD95 death receptor, leading to receptor oligomerization and the formation ... polymers in response to damaged DNA Caspases also terminates several proteins involved in maintenance of the cytoskeletal architecture such as the intermediate filaments cytokeratin-18 and vimentin...

Ngày tải lên: 11/09/2015, 09:05

... for us I am indebted to many of my colleagues for providing a stimulating and fun environment in which to learn and grow I’m especially grateful to Suey Li, Shue Yin, Lin Ming, Ramesh and Chi Wen ... materials technology and is beginning to impact the new frontier of spintronics Epitaxial Ni/insulator interfaces are of growing interest, and their application concerns many different industrial sectors, ... lattice points11 and found has a larger intersecting volume than texture, thus indicating that lowers the overall misfit and the interfacial energy, in addition to accommodating the...

Ngày tải lên: 09/10/2015, 11:24

... current research work It involves developing a frame work for modeling and analyzing microbial and mammalian systems using a combined statistical and in silico approach to gain insights about the effect ... consider, analyze, and ultimately understand the living world, whereas physics and chemistry come handy in probing the behavior of molecules and their activity inside living cells Engineering applications ... Since it is an intrinsically interdisciplinary research, it involves expertise and perspectives from different disciplines such as engineering, biology, computer science, physics, chemistry and...

Ngày tải lên: 11/09/2015, 21:27

... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... binding and nuclear localization), which is shared by all FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved...

Ngày tải lên: 16/02/2014, 09:20

... N-acetylornithine deacetylase; OCT, ornithine carbamoyltransferase; ASS, argininosuccinate synthase and ASL, argininosuccinate lyase by arginine [16,17] As a result, the concentration of citrulline and ... mitochondria [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic ... insensitivity to the inhibition induced by citrulline and arginine However, many different enzymes are involved in the metabolism of citrulline (Fig 1), and their kinetic properties and ⁄ or expression...

Ngày tải lên: 20/02/2014, 03:20

... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing ... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids long; ... according to the manufacturer’s instructions The protein was eluted with buffer K containing M imidazole and cleaved by thrombin (Novagen) at room temperature for 17–20 h, using a protease/protein...

Ngày tải lên: 22/02/2014, 07:20

... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... (S)-nandinine from (S)-scoulerine in vitro; indeed, CYP719A3 converted (S)-scoulerine to (S)-nandinine with high affinity (Km ¼ 0.54 lm) (Fig 6) Previous reports have shown that (S)-stylopine is ... CYP719A2, and CYP719A3 was all the highest in root, and sanguinarine is found exclusively in root (data not shown), root is likely the main organ for the biosynthesis and storage of sanguinarine in...

Ngày tải lên: 07/03/2014, 10:20

... DNA binding domain Ligand binding domain Fig Sequence alignment (A) Alignment of DNA binding domain (C domain) and its C-terminal extension (B) Alignment of ligand binding domain (E domain) (after ... frame of 2145 bp corresponding to a 715 amino acid protein The DNA binding domain (DBD) is highly conserved, the P-box (EGCKG), which is involved in determining DNA binding specificity, is identical ... important role in determining SmNR1 ⁄ SmRXR1 heterodimer binding to the DNA element That amino acids in the A ⁄ B domain can affect DNA binding and dimerization has previously been reported in the chicken...

Ngày tải lên: 07/03/2014, 11:20

... [19], and papain [11], VS-A, VS-B and VXH-A lack the insertion of four amino acids between position 168 and 169, which is present in all other cysteine proteinases of plant or animal origin Unfortunately, ... P2, Q19 and the 11GAVTP15-motif located at the N-terminus of papain-like cysteine proteinases, leaving no doubt that these proteins belong to the papain superfamily Sequencing of VXH-I and VXH-III ... manufacturer’s instructions Insert sequencing was carried out in both directions using T7 and SP6 primers Inserts were sequenced using the ABI prism BigDyeTM Terminator v1.1 Cycle Sequencing Kit (Applied...

Ngày tải lên: 07/03/2014, 11:20

... resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C terminus of the protein belong to different subunits and condense to form an intersubunit disulfide bond; in atypical ... the N terminus and SR at the C terminus belong to different subunits and condense to form an intersubunit disulfide bond; in atypical 2-Cys, Sp at the N terminus and SR at C terminus, originate from ... or protein instability caused by oxidative stress conditions, are important in determining the level of Bcp2 protein Similar results are observed for genes and related proteins involved in oxidative...

Ngày tải lên: 07/03/2014, 12:20

... ML3p, ML3.1p (Figs and 2) B-chains The ML1p and ML2p B-chains, like the rML B-chain, are 263 amino acids in length and the ML3p B-chain is 266 amino acids in length owing to the insertion of RGT(128130) ... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu152; and Met234 of the ML1p and ML2p B-chains corresponding to the ricin B-chain Leu233) Estimation ... efficiently binds to ML1p and ML2p A-chains and reacts poorly with the ML3p A-chain (Fig 6) Binding of the recombinant MLp A-chains with TA7 was detected in all samples Interaction of the native A-chains...

Ngày tải lên: 07/03/2014, 15:20

... made; in the last wash, the resin containing the recombinant proteins was loaded into a gravity-ow column The column was washed once with lysis buffer containing 10 mm imidazole The proteins were ... within days Inactivation of recombinant EhPPDK by cold storage was previously observed during storage in 50 mm imidazole [11] However by storing EhPPDK in 50% (v v) glycerol at )20 C, a 50% increase ... ATP in several reactions It has a PPi-dependent phosphofructokinase (PPiPFK) [6,7] and a pyruvate phosphate dikinase (PPDK) [8,9], and the partial kinetic characterization of these recombinant...

Ngày tải lên: 07/03/2014, 17:20

... Oncogenes and Related Genes Shanghai Cancer Institute, Shanghai, China Smith RK, Carroll PM, Allard JD & Simon MA (2000) MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila ... variant A Fig Interaction of Vpr and VBARP proteins in vitro and in vivo (A) In vitro translated 35S-labeled VBARP-His isoforms were incubated with Vpr or Nef and immunoprecipitated using a-His (lanes ... ankyrin repeat proteins, using the consensus established by Kohl et al [10] and Mosavi et al [11], which contains ankyrin repeats from over 4000 proteins, indicated that the ankyrin repeat domains...

Ngày tải lên: 07/03/2014, 21:20

... Nucleotide and deduced amino acid sequences of HRT1 (A) and HRT2 (B) Numbers of nucleotide sequence and amino acid sequence are indicated on the left and right, respectively The underline in HRT1 indicates ... template and then sequenced Finally, two cDNAs were obtained and designated HRT1 and HRT2, respectively DNA sequencing analysis Materials and methods Plant materials and RNA isolation Latex and various ... fragments and obtained two distinct cDNAs that contain sequences identical to those obtained by 3¢-RACE General cDNAs containing 5¢- and 3¢-untranslated regions were also amplified by RT-PCR and sequenced...

Ngày tải lên: 07/03/2014, 21:20

... expected initiation methionine by translating the fusion protein GST-XN73 and His6-XN73 As can be seen in Fig 2B, these fusion proteins run at the expected height, proving that no internal methionine ... proteins from an in vitro transcribed RNA injected into X laevis oocytes High expression is obtained as a consequence of inserting the cDNAs between the 5¢- and 3¢-UTR of b-globin, a protein that ... gov/blast/) and the ÔMULTALINÕ alignment program (http:// prodes.toulouse.inra.fr/multalin/multalin.html) Human genome sequences were obtained by doing BLAST searches against the HTGS database and the...

Ngày tải lên: 08/03/2014, 08:20

... A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M & Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin ... into pBluescript SK(–) (Stratagene) by in vivo excision, and was sequenced in both strands by the dideoxy chaintermination method using a model 373A DNA sequencer (PE Biosystems) Nucleotide and ... Pharmacia Biotech Plant material and culture conditions Cell suspension cultures of Luffa [16] were maintained in a 300-mL Erlenmeyer flask containing 60 mL Linsmaier and Skoog medium [23], supplemented...

Ngày tải lên: 08/03/2014, 23:20