identification and functional characterization of srnas in neisseria meningitidis

... of the binding sites of the AP2-domain containing transcription factors as inferred from protein-based microarray studies conducted in P falciparum [28] In a study of the promoter strengths of ... intriguing results Genes involved in nucleotide biosynthesis and salvage Purines and pyrimidines are the building blocks of nucleic acids in living cells All protozoan parasites examined thus far are ... occurrences of each candidate motif within coding and upstream regions Upstream Motif Number of genes Number of motifs Coding Number of motifs/10 kb Number of genes Number of motifs Number of motifs/10...

Ngày tải lên: 14/08/2014, 21:20

... science and directionality of projects His broad scope of scientific interests, inspiring ideas, critical thinking, deep penetrance of scientific investigations and many other outstanding scientific ... proteinRNA complexes with the RNA-induced silencing complex (RISC) and bind to either 3’UTR or coding regions of messenger RNAs, causing destabilization of mRNA and/ or inhibition of protein translation ... As shown in Fig 1.2, Ago are large proteins about 100kDa comprising a single     variable N-terminal domain and three conserved C-terminal domains, including the PAZ, MID and PIWI domains (Vaucheret,...

Ngày tải lên: 09/09/2015, 10:17

... VBARP and Vpr is specific The input panel represents the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction ... variant A Fig Interaction of Vpr and VBARP proteins in vitro and in vivo (A) In vitro translated 35S-labeled VBARP-His isoforms were incubated with Vpr or Nef and immunoprecipitated using a-His (lanes ... a role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK...

Ngày tải lên: 07/03/2014, 21:20

... volume and energetic properties of the binding of CO to hemoproteins Biophys J 66, 89–98 Tuckey RC & Kamin H (1983) Kinetics of O2 and CO Binding to adrenal cytochrome P-450scc Effect of cholesterol, ... was in the range of values determined in previous studies for the interaction between bovine Adx and bCYP11B1 Biacore measurements were performed to investigate the binding behavior between bovine ... transfer chain consisting of a [2Fe-2S] ferredoxin, adrenodoxin (Adx) and a NADPH-dependent, FAD containing reductase, adrenodoxin reductase (AdR; EC 1.18.1.2) [8,9] This electron transfer chain is...

Ngày tải lên: 30/03/2014, 04:20

... in intracellular cytoskeletal proteins, including titin, MyBP-Cm, MyBP-M, MyBPH, myotilin, and palladin [30–35] As Ig-C2 domains may serve as molecular spacers and bind to a diversity of ligands, ... domain in myosin light chain kinase A Fig Prediction for a conserved aggregation domain in the 4Ig region of L-MLCK and recombinant expression of MLCK variants (A) The sequences of the N-terminal ... barrier in intestinal disease Lab Invest 84, 282–291 12 Tran QK, Watanabe H, Zhang XX, Takahashi R & Ohno R (1999) Involvement of myosin light-chain kinase in chloride-sensitive Ca2+ in ux in porcine...

Ngày tải lên: 30/03/2014, 04:20

... results again highlight the stimulatory role of P2Y12 in mitogen-activated protein kinase and Akt kinase signaling pathways On the other hand, the functional activity of the P2Y1 receptor in long-term ... to fragmented adhesion region in the cell center Vinculin staining on the whole cell surface in most of the cells (green, G); some cells still have vinculin staining similar to starved cells (arrow) ... chemiluminescence Equal loading of proteins was ensured by b-actin concentration Control: cells were growing in MEM containing 10% fetal bovine serum 24, 48, 72, 96 h: cells were cultivated in MEM...

Ngày tải lên: 30/03/2014, 08:20

... library using a combination of PCR amplification, mass sequencing and library screening as described in the Materials and methods The first clone was 1689 bp and contained an ORF of 1434 bp coding for ... selection on minimal medium agar plates without uracil or leucine, cells harbouring the yeast plasmid were cultivated in minimal medium lacking uracil or leucine but containing 2% (w/v) raffinose and 1% ... manufacturer’s instructions and submitted to random sequencing by the chain termination method using the ABI PRISM Big Dye Termination Cycle Sequencing Ready Reaction Kit (Perkin-Elmer, Weiterstadt,...

Ngày tải lên: 31/03/2014, 09:20

... apolipoprotein E J Biol Chem 2003, 278:40723 Gregg RE, Brewer HB Jr: The role of apolipoprotein E and lipoprotein receptors in modulating the in vivo metabolism of apolipoprotein Bcontaining lipoproteins ... their charge [43] In these experiments, the interaction of VLDL and apoE proteins increased the charge of VLDL particles, resulting in the migration of VLDL band (lane vs lane 3-4 in Figure 4) Remarkably, ... lipoprotein receptorbinding activity of the human E apoprotein due to cysteine-arginine interchange at a single site J Biol Chem 1982, 257:2518 Lane RM, Farlow MR: Lipid homeostasis and apolipoprotein...

Ngày tải lên: 10/08/2014, 05:21

... distributed in the cytosol of CP70 control cells (Figure immunostaining) Overexpression of Trip10 in IMR-32 cells caused Trip10 and huntingtin to colocalize and form perinuclear foci In contrast, ... tumorigenesis and metastasis in mice inoculated with Trip10-overexpressing IMR-32 cells (Figure 4A) On the other hand, huntingtin increases cell death by promoting apoptosis Thus, high levels of huntingtin ... profound effect in the cell type-specific functions of Trip10 Discussion Trip10 was initially identified as a Cdc42-interacting protein involved in GLUT4-mediated glucose uptake in adipocytes and...

Ngày tải lên: 10/08/2014, 05:21

... Expression of recombinant HCT in E coli Expression of recombinant HCT in E coli Protein content of non-induced (T = h) and induced (T = h) non-transformed cells (1) were compared with those of non-induced ... quantity of HCT transcript and the content of caffeoylquinic acids was observed in all accessions Since HCT silencing induces an increase (or no significant change) in the amount of caffeoylquinic ... choleretic, diuretic and bile-expelling activities [4], (iv) the inhibition of HIV integrase, a key player in HIV replication and its insertion into host DNA [14,15], and (v) antibacterial and antifungal...

Ngày tải lên: 12/08/2014, 05:20

... material, increased production of reactive oxygen and nitrogen species and of the pleiotropic cytokine TNF-α In contrast, they release reduced amounts of the proinflammatory cytokines IL-1β and IL-6 and ... antibodies were detected using a biotin-PE/streptavidinanti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter ... enzyme-specific contributions of matrix metalloproteinases-9 and -12 in IL-13induced inflammation and remodeling J Clin Invest 2002, 110:463-474 Haro H, Crawford HC, Fingleton B, Shinomiya K, Spengler...

Ngày tải lên: 12/08/2014, 16:20

... minimum six months of a combination of four antibiotics containing rifampicin, isoniazid (INH), pyrazinamide (PZA) and ethambutol (EMB) for the first two months, followed by only rifampicin and ... formation is initiated by recruitment of immune cells to the site of infection by range of cytokines and chemokines that are released in response to the infection Among the cytokines, tumor necrosis ... Husna, Jacklyn, Jingyan and Robin A big thank you to all of you for being such amazing friends I would like to thank my family members for their affection and understanding and my best friend...

Ngày tải lên: 09/09/2015, 10:07

... domain of Lrp5/6 contains Axin2 binding sites (Mao et al., 2001) Thus, binding of Axin might be the trigger for inactivation of the phosphorylation of β-catenin The binding sites are five reiterated ... great care of our fish I would also like to extend my gratitude to the Republic of Singapore and its decision making bodies for creating and maintaining this wonderful country in the heart of Southeast ... according to Walker and Kimmel (2007): Larvae were fixed in 4% PFA for 2h, washed in PBST and incubated in 50% ethanol for 10 minutes Subsequently, larvae were incubated over night in staining...

Ngày tải lên: 10/09/2015, 08:43

... Paramyosin 12 13 Unknown Fatty acid binding protein 14 Apolipophorin 177 98kDa chitinase Gelsolin-like protein/villin Ca binding EF-hand 60kDa chitinase Anti-microbial peptide homologue Arginine kinase ... mutagenesis of selected amino acid residues lining the cavity of Der p was used to investigate their role in cholesterol binding Alanine mutation of eleven amino acid residues lining the cavity of Der ... sensitization profile 142 Figure 6.9 IgE binding of the 21 single alanine mutants of Der p in individuals of group (ii) sensitization profile 144 Figure 6.10 Location of important IgE binding residues...

Ngày tải lên: 15/09/2015, 17:09

... protein Similarly, both the R186 residue in the Rep245 domain and K300, its counterpart in Pfu-primase, were contained in a loop that is plausibly involved in DNA recognition and binding and is ... 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... activity of Rep245 and Rep516 proteins (A) RNA primer synthesis Reaction mixtures, containing M13 singlestranded circular DNA, NTPs including [32P]ATP[aP], and Rep245 (or Rep516), were incubated...

Ngày tải lên: 18/02/2014, 18:20

... obtained by simple rotation of the same model The flexible LID domain appears at top of the three figures, NMP-binding site and ATP binding domain are displayed, NH2- and COOH-termini are also indicated ... growth curve of promastigotes Functional characterization of recombinant AK The recombinant protein purified from the inclusion bodies and refolded in the presence of dithiothreitol, MgSO4 and KCl ... the amino acid sequence of AK2 protein The purification and functional folding of L donovani AK from the E coli cell lysates was carried out by washing and dissolving the inclusion bodies in M...

Ngày tải lên: 21/02/2014, 00:20

... extracellular domain of corticotropin 2830 A Pisarchik and A Slominski (Eur J Biochem 271) 30 31 32 33 releasing factor-R1 contains major binding determinants for urocortin and astressin Endocrinology ... volume of DMEM media containing 10% fetal bovine serum was added and cells were incubated overnight Next morning, the cells were washed by DMEM and incubated in DMEM media containing 5% fetal bovine ... urocortin The artificial isoform (CRFR1h2) with the insertion of 37 amino acids between ligand binding domain and the first extracellular loop was able to produce 2828 A Pisarchik and A Slominski...

Ngày tải lên: 07/03/2014, 15:20

... Sepharose, and the bound GST fusion protein was treated with mL of a thrombin digestion buffer (50 mM Tris/HCl pH 8.0, 150 mM NaCl, 2.5 mM CaCl2) containing UÆmL)1 bovine thrombin Following a 30-min incubation ... tyrosineO-sulphate-binding protein and tyrosine-sulphated proteins in bovine liver membrane lysates Biochem J 275, 259–262 16 Sanger, F., Nicklen, S & Coulson, A.R (1977) DNA sequencing with chain-terminating ... fetal bovine serum, 0.5% trout serum, 0.1 mgÆmL)1 bovine insulin, 50 lgÆmL)1 streptomycin sulfate, and 30 lgÆmL)1 penicillin G Confluent zebrafish liver cells grown in individual wells of a 24-well...

Ngày tải lên: 08/03/2014, 02:20

... amino acids function as the signal peptide that has been cleaved off in the mature protein The amino acid sequences of the N-terminal and three internal peptide fragments of Mrcp-19k determined ... alkylated gold The arrows and thick arrows indicate the starts of sample loading (2 lM) and washing by the running buffer, respectively The processes of sample loading and washing were sequentially ... In a relevant protein, the antifreeze protein, which binds to the ice nucleus to inhibit crystal growth in the cytosolic space of several organisms, including bacteria and fish [17], the Ala and...

Ngày tải lên: 16/03/2014, 05:20

... shown in the presence of the actin crosslinking protein neurabin-2 (D) and an actin binding decient truncation mutant of neurabin-2 (E) in the lament cross-linking efciency of Iba1 and Iba2 or in ... phagocytosis [3] and to enhance the interaction of Iba1 with F-actin to a certain degree [11] In this study F-actin binding and cross-linking by Iba proteins was calcium independent, conrming previous ... morphology of the generated lament bundles Calcium afnity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar in the absence or presence of calcium ions Calcium binding in...

Ngày tải lên: 16/03/2014, 06:20