human sperm chromatin structure and function

... therefore focus primarily on the actual chromatin structure of active genes, with regard to nucleosomal organization and higher order structure, and the chromatin structure changes that occur during ... (1990) Chromatin structure of transcriptionally competent and repressed genes EMBO J 9, 3997–4006 97 Woodcock CL, Skoultchi AI & Fan Y (2006) Role of linker histone in chromatin structure and function: ... 30-nm chromatin fibres versus open 10-nm chromatin fibres We also need to be able to define the specific mechanisms that create a more dynamic chromatin structure in which nucleosomes and chromatin...

Ngày tải lên: 14/02/2014, 18:20

... C1R (PDB code: 1GPZ [27]) and of the modelled structures of HPT1 and HPT2 HPT1 residue Cys15 and HPT2 residues Cys15 and Cys74 are shown in spacefill representation This and the following figures ... and C1R (A), and between HPT2 and C1R (B) Conserved residues are shaded in grey; Cys residues are highlighted in yellow Above the sequence alignment, red, green and magenta bars indicate a- and ... Hp1F and Hp1S alleles, and is present only in humans [22,23], although similar but independent events have been very recently evidenced in other mammals such as deer and cow [24,25] The human...

Ngày tải lên: 23/03/2014, 06:20

... form and function of novel complexes such as HAMLET and ELOA, we have provided new evidence that a loss of native structure can endow proteins and their complexes with distinct and beneficial functions ... successfully target and kill tumor cells in humans and animals In view of this extensive documentation, we propose that it would be useful if HAMLET were used Structure and function of HAMLET-type ... ago and since then the HAMLET field has widened in scope, acquiring new members and enriching our understanding of the basic principles underlying protein self-assembly and acquisition of new functionality...

Ngày tải lên: 29/03/2014, 21:20

... To determine the Apo and complex structures of these proteins with ligands (AdoMet/SAM and AdoHcy/SAH) using X-ray crystallography Structure function analysis to understand the role of these ... intrinsically unstructured protein and they are highly flexible and lack the globular fold However they are functionally active proteins in vivo and in vitro conditions Further we report the crystal structure ... Materials and Methods 4.2.1 Cloning, expression and protein purification 4.2.2 Crystallization and structure determination 4.2.3 Isothermal titration calorimetry 4.2.4 PDB Code 4.3 Results and discussion...

Ngày tải lên: 10/09/2015, 08:38

... Location Biotics and abiotics Energy and material flow Human impacts, consequences and solutions Conclusion LOCATION  Thong Nhat Park  Peninsula : 1200 m2  Including : mainland, lake, plants, ... restaurant in this area and gases from vehicles on Le Duan road HUMAN IMPACTS - CONSEQUENCES SOLUTIONS  Increasing the number of wastebasket  Planning the rubbish dumps and quick transporting ... the restaurant and visitors to keep the park green, clean and beautiful CONCLUSIONS  Plant species on peninsula are quite diverse  People are having huge impacts on the formation and development...

Ngày tải lên: 28/05/2016, 09:28

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned into ... the interaction between v-KIND and MAP2 (A) Alignment of the KIND2-1 region in human, mouse and Gallus (top) and the corresponding mouse v-KIND KIND1, Spir-2 KIND and PTPN13 KIND domains (bottom) ... clarified the structural and functional importance of the v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neurons and cerebellar granule...

Ngày tải lên: 14/02/2014, 19:20

... marine and freshwater ecosystems James H Brown, Andrew P Allen and James F Gillooly Body size and suspension feeding Stuart Humphries 16 Life histories and body size 10 David Atkinson and Andrew ... empirical and theoretical work in biological oceanography and limnology that relates the structure, function and biotic composition of these systems to the body sizes of the organisms present and the ... be done, and the breadth and depth of understanding that they can potentially contribute Through fisheries, pollution, climate change and other impacts, humans are transforming marine and freshwater...

Ngày tải lên: 17/02/2014, 19:20

... APs References Davies, D.R (1990) The structure and function of the aspartic proteinases Annu Rev Biophys Biophys Chem 19, 189–215 Dunn, B.M (2002) Structure and mechanism of the pepsin-like family ... proteins: structure, function, and role in human lysosomal storage disorders FASEB J 5, 301–308 38 Munford, R.S., Sheppard, P.O & O’Hara, P.J (1995) Saposinlike proteins (SAPLIP) carry out diverse functions ... many different and diverse functions, including specific protein processing (e.g rennin, cathepsin D and yapsins), protein degradation (e.g gastric enzymes such as chymosin, pepsin and gastricsin)...

Ngày tải lên: 19/02/2014, 12:20

... investigation and understanding of their structure- activity relationships, may start to provide a rational way to develop additional pharmacological tools for the elucidation of nAChR structure and function ... oocyte-expressed human nAChR subunit combinabrain [64] and has proven to be a powerful radioligand in tions and seems to have a similar selectivity and activity as numerous binding and autoradiography ... protein has structural and functional homology to the extracellular ligand binding domain of the nAChR, and likewise assembles into pentamers Ó FEBS 2004 unable to form functional channels in...

Ngày tải lên: 19/02/2014, 12:20

... Graether and B D Sykes (Eur J Biochem 271) Fig Fish AFP structures and model The structures of the fish AFPs are shown as ribbon diagrams with coil structure shown as yellow, a-helices as red and b-strands ... [31,32,36] and their threedimensional structures were determined [34,37–40] In subsequent sections, we describe the structure and dynamics of each protein, and present a comparison of sbwAFP and TmAFP ... other and with proteins that have a similar fold Structure of sbwAFP and TmAFP The structure of sbwAFP has been determined by X-ray ˚ crystallography to 2.5 A and by NMR at both 30 °C and °C...

Ngày tải lên: 19/02/2014, 16:20

... three antiparallel strands (1, and 7) and strand 6, which is parallel to strand b-Strands are located between the four a-helices (a1, 278–294; a2, 305–313; a4, 374–381; and a5, 397–405), with ... and quaternary complexes in solution with GTP and Mg2+ (eRF1–eRF3–GTP and eRF1–eRF3–GTP– Mg2+) [17] Yeast two-hybrid and deletion analyses have revealed that residues 281–305 and 411–415 of human ... the structure of the protein core (residues 277–328 and 373–413) in solution and in the solid state, and indicates their similarity A superposition of the C-domain NMR structure on the crystal structure...

Ngày tải lên: 06/03/2014, 11:20

... crystal structure coordinates for human transthyretin (including multiple amyloidogenic forms and with various ligands bound) The crystal structures of transthyretin from rat [35], chicken [36] and ... between strands E and F in transthyretin The two transthyretin dimers associate, via nonpolar interactions, between the loops joining stands G and H with the loops joining strands A and B, making ... between the structures of TLP and transthyretins are found in the loop connecting b-strands B and C, which is highly exposed to the solvent in TLP [17] Interruptions in the b-strands A, G and H are...

Ngày tải lên: 07/03/2014, 00:20

... antiparallel b-strands is abutted by three a-helices (a1, a2, and a¢) The b-sheet in type I KH domains consists of three b-strands in the order b1, b¢ and b2 The b1-strand and b2-strand are parallel ... Valverde et al Structure and function of KH domains A B C Fig 10 Crystal structure of tandem type II KH domains of NusA in complex with RNA The tandem KH1–KH2 domains of NusA recognize RNA ligand 5¢-GAACUCAAUAG ... the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adjacent and antiparallel to the b1-strand (Fig 1) The length and sequence of the variable loop are different...

Ngày tải lên: 07/03/2014, 05:20

... Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur J Biochem 269) 4213 Fig De novo and salvage pathway of UDP-GlcNAc biosynthesis EXPERIMENTAL ... number of functionally relevant cysteines was quantified to two, and dithiol-modifying reagents predicted a structural vicinity of these cysteines The amino acid sequences of murine and human GlcNAc ... independent expressions Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur J Biochem 269) 4215 able GlcNAc kinase activities, indicating a successful functional expression However, these...

Ngày tải lên: 08/03/2014, 10:20

... double-stranded DNA λ phage 48,500 Linear double-stranded DNA T7 phage 40,000 Linear double-stranded DNA M13 phage 6,400 Closed-circular single-stranded DNA MS2 phage 3,600 Linear single-stranded RNA Human ... in a mRNA molecule that is singlestranded RNase P M1 RNA RNA molecules not have a regular structure like DNA hairpin Structures of RNA molecules are complex and unique RNA molecules can base pair ... 6,000,000,000 Linear double-stranded DNA Fruit fly 270,000,000 Linear double-stranded DNA HIV 9,700 Linear single-stranded RNA DNA molecules are packaged in the cell as structures called chromosomes...

Ngày tải lên: 13/03/2014, 16:42

... larger:   “Frilly” edged…… Long and narrow…  Round cells will always be small Prokaryotic Cell Structure  Prokaryotic Cells are smaller and simpler in structure than eukaryotic cells   ... cells    why are cells so small? Prokaryotic cells Eukaryotic cells Cell size and shape –    Organelles and structure in all eukaryotic cell Organelles in plant cells but not animal Cell ... Membrane bound organelles Prokaryotic Cell Structure  Structures       Plasma membrane Cell wall Cytoplasm with ribosomes Nucleoid Capsule* Flagella* and pili* *present in some, but not all...

Ngày tải lên: 13/03/2014, 19:31

... RER Functions of Eukaryotic Cell Features Structure Function( s) Golgi Apparatus Collects, modifies, packages and distributes proteins and lipids from ER Functions of Eukaryotic Cell Features Structure ... cell? Functions of Eukaryotic Cell Features Structure Function( s) Regulates passage of Plasma materials into and out of cell Membrane Cell-Cell Recognition Functions of Eukaryotic Cell Features Structure ... Plasma Membrane Functions of Eukaryotic Cell Features Structure Peroxisome Function Removes harmful oxidants from cells Functions of Eukaryotic Cell Features Structure Mitochondrion Function Captures...

Ngày tải lên: 13/03/2014, 19:31

... buffer, pH 7.0, and its UV spectra were recorded between 250 and 190 nm Molecular modelling Structure manipulations and analysis were performed using the PYMOL software [41] Results and Discussion ... buffer, pH 7.0, and eluted with 0.1 M potassium phosphate buffer, pH 7.0, containing mM GSH Recombinant rat GST A1-1 [32] and human GST A1-1 [33] were expressed in Escherichia coli and purified on ... (for rat GST A1-1 and human GST A1-1) or 100 lmol potassium phosphate buffer, pH 7.5 (for glutathione reductase, glutathione synthase and L-lactate dehydrogenase), SDTG (98.2 nmol) and enzyme (typically...

Ngày tải lên: 16/03/2014, 16:20

... Education, Science, and Culture of Japan, and a fellowship from Katayama Chemical Industries, Co., Ltd (Osaka) to T.Y Ó FEBS 2004 Effects of DiS-C on mitochondrial structure and function (Eur J Biochem ... found DiS-C3(5) to show multiple effects on the mitochondrial structure and function, effects 11 dependent on both its concentration and the Pi status Acknowledgements This work was supported by ... interpretation, we further characterized the actions of DiS-C3(5) on the mitochondrial structure and function and compared them with those of Ca2+, known as a typical PT inducer Like that of Ca2+,...

Ngày tải lên: 16/03/2014, 18:20

... in Table 1, C or I, and, to some extent, also I* and C* The lower stability of Hc from nonpeneid Crustacea yields a structure where divalent ions (such as Ca2+ and Mg2+) and pH regulate the oligomerization ... Beltramini et al P monodon hemocyanin structure and function equilibrium constant, lo, describes the ratio of conformations r and t in the absence of ligand: lo ¼ ½t0 Š ½r0 Š Three-state MWC ... 2060–2075 ª 2005 FEBS P monodon hemocyanin structure and function 49 50 51 52 53 54 55 56 57 58 59 60 61 oxygen binding and interactions with l-lactate, calcium, and protons Biochemistry 27, 1995–2001...

Ngày tải lên: 23/03/2014, 13:20