human embryonic stem cells in drug discovery pdf

... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may ... meiotic spindle after enucleation However, using a squeezing method, they are Human embryonic stem cells and therapeutic cloning successful in obtaining monkey cloned blastocysts [67], supporting our ... AM, Inzunza J, Hreinsson J, Rozell B, Blennow E, Andang M, Ahrlund-Richter L A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells Human...

Ngày tải lên: 07/08/2014, 18:21

... is in need In this book, we explore the possibility of using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells ... either from murine or human origin In this study, we investigated the efficiency of purmorphamine in differentiating human embryonic stem cell into osteoblast lineage by comparing with the traditional ... somatic cells They repeatedly introduce certain proteins channeled into the cells via poly-arginine anchors and sufficiently induced pluripotency[45] This new technique brings in new hope in stem...

Ngày tải lên: 16/10/2015, 15:38

... neuroprotection in the ischemic brain human embryonic stem cells diferentiationon OP9 feeder Day 10 Day VEGF-R2(+) / VE-cadherin(+) / TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion ... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the...

Ngày tải lên: 18/06/2014, 15:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are ... Garg KS, Parkin RK, Kroh EM, Bendoraite A, Mitchell PS, Nelson AM, et al.: MicroRNA Discovery and Profiling in Human Embryonic Stem Cells by Deep Sequencing of Small RNA Libraries Stem Cells 2008, ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation-related...

Ngày tải lên: 18/06/2014, 15:20

... type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The President's ... approaches, in a manner to facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Human embryonic ... of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines will promote...

Ngày tải lên: 13/08/2014, 23:20

... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable ... importance of SHH in early mammalian development, there is significant value in studying its function in human embryonic stem cells (hESC), which are in vitro counterparts of the inner cell mass...

Ngày tải lên: 11/09/2015, 10:14

... staining (b) H1 von Kossa staining (c) hFOB H & E staining (d) hFOB von Kossa staining Figure At 4-week time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB ... time-point: (a) (c) nuclear staining with DAPI (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells...

Ngày tải lên: 09/10/2015, 11:24

... of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington, MA, ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical assisted reproduction [15,...

Ngày tải lên: 31/10/2012, 16:57

... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, ... differentiation, blast cells have been resuspended in EGM-2 complete media (Cambrex) and incubated in fibronectin coated plates (Becton Dickinson) for days To prove that fibronectin-adhering cells are of ... genes In order to find the exact time point when blast colonyforming cells (BL-CFCs) arise in the EB system, we started a series of BL-CFC cultures on days to of EB differentiation in vitro In our...

Ngày tải lên: 18/06/2014, 15:20

... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, ... Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36 ...

Ngày tải lên: 11/08/2014, 12:21

... of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types Reprod Biomed Online ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... and in vivo mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic...

Ngày tải lên: 11/08/2014, 20:21

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon interaction ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...

Ngày tải lên: 11/09/2015, 09:16

... - GDF Haematoxylin and eosin - H&E Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal ... hESCs In the study, hESCs were induced to form EBs in serum-supplemented medium for days prior to dissociation into single cells and co-culturing with human nasal chondrocytes in a transwell insert ... Alcian blue staining……………………………………………… 56 3.7.2.4 Safranin-O staining………………………………………………… 56 3.7.2.5 Masson’s trichrome staining……………………………………… 57 3.7.2.6 Immunohistochemical staining……………………………………...

Ngày tải lên: 11/09/2015, 09:57

... classified into six broad groups according to their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem ... linked to proteins in the form of proteoglycans The other is fibrous proteins, including collagen, fibronectin and laminin, etc Proteoglycan is thought to have a major role in chemical signaling ... culture and maintenance of hESCs on human laminin in culture medium supplemented with either activin A (Beattie et al., 2005) or a cocktail of growth factors, including human FGF-2, human stem cell...

Ngày tải lên: 11/09/2015, 10:00

... fluorescent protein ES cells FBS hES cells embryonic stem cells fetal bovine serum human embryonic stem cells HIV human immunodeficiency virus HSV herpes simplex virus ITR inverted terminal repeat ... choosing various promoters and viral modulators to achieve desirable level of transient transgene expression in human embryonic stem cells Efficient gene transfers to human embryonic stem cells ... Finally, the effects of the baculoviral transduction of the human embryonic stem cells were investigated The transduction of human embryonic stem cells by baculovirus vectors had no effects on the cell...

Ngày tải lên: 11/09/2015, 14:38

... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... experiments involving blastocyst injections and ectopic grafting in adult hosts cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains ... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their...

Ngày tải lên: 16/09/2015, 15:55

... CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective ... immunosuppressive drugs like tacrolimus (binds calcineurin and thereby inhibits T-cell signaling ) and sirolimus (blocks 11 activation of T- and B- cells by inhibiting interleukin-2 responsiveness) ... hESCs maintained on human feeder cells are amenable to haematopoietic differentiation 80 3.3.1 hESCs maintained on CCD919 cells 82 3.3.2 hESCs maintained on Ortec143 cells ...

Ngày tải lên: 04/10/2015, 16:03

... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells ... cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It was originally found to regulate ... IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced...

Ngày tải lên: 16/10/2015, 11:58

... RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure ... polymerase Chain Reaction (rtPCR) 29 Figure hESCs maintained at 37oC (physiological control) by PNA FISH 33 Figure Figure hESCs maintained at oC for 24h by PNA FISH hESCs maintained at 25 oC ... However, established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been...

Ngày tải lên: 22/10/2015, 21:13

... Cytokines and BMP-4 promote hematopoietic differentiation ofhuman embryonic stem cells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Human embryonic stem cells maintained in ... embryonic stem cells using lentiviral vectors Stem cells 2003, 21:111-117 Gropp M, Itsykson P, Singer O, Ben-Hur T, Reinhartz E, Galun E, Reubinoff BE: Stable genetic modification of human embryonic stem ... from human embryonic stem cells exhibit an embryonic gene expression pattern Blood 2004, 103:4134-4141 Martin CH, Kaufman DS: Synergistic use of adult and embryonic stem cells to study human...

Ngày tải lên: 13/08/2014, 09:21