maintenance of chicken embryonic stem cells in vitro pdf

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... protein folding in physiological environments J Mol Biol (3):469-482 76 Harve Vigneshwar Rajagopalan R, KS, R, et al 2008;Macromolecular crowding in vitro as means of emulating cellular interiors:...

Ngày tải lên: 11/09/2015, 09:16

... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines...

Ngày tải lên: 13/08/2014, 23:20

... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation-related ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...

Ngày tải lên: 18/06/2014, 15:20

... time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells 43 Figure At week time-point: (a) H&E staining showing newly formed tissue within the ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB ... time-point: (a) (c) nuclear staining with DAPI (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells...

Ngày tải lên: 09/10/2015, 11:24

... layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington, ... use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical ... of ATP in the dynamics of the actin filaments of the cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low body temperatures on the degree of polymerization of...

Ngày tải lên: 31/10/2012, 16:57

... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... marks to the b-globin gene locus during erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima ... differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points All primer...

Ngày tải lên: 07/03/2014, 12:20

... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... CCCTC-binding factor, insulator associated protein; ESC, embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of...

Ngày tải lên: 11/08/2014, 12:21

... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... to 0.4 mm in diameter was established Feeder-independent murine embryonic stem cells (ESCs) were kindly provided by K Pfeffer (Institute for Microbiology, Heinrich Heine University of Düsseldorf, ... prominent in the centre of the sphere, as demonstrated in histological sections stained with Figure Micromasses consisting of embryonic stem cells were cultured with or without DAG and stained...

Ngày tải lên: 11/08/2014, 20:21

... functions of individual candidates in influenza pathogenesis will require further investigation Lack of the control of transcriptional suppression by zinc finger proteins in pdmH1N1 infected cells ... nine of the genes down-regulated in response to seasonal H1N1 influenza virus (but not pdmH1N1) encode zinc finger proteins, including ZNF175 ZNF175 contains 13 zinc fingers and a KRAB domain, ... seasonal influenza viruses in its induction of cytokine responses in human macrophages and epithelial cells [2-4] This suggests that the cytokine dysregulation seen in H5N1 infection is not an intrinsic...

Ngày tải lên: 12/08/2014, 11:22

... gastrointestinal tract of a guinea pig, a rabbit, a cow, five mice, six pigs, or 12 chickens [15] Interestingly, SdiA was activated in mice that were infected with the AHL-producing pathogen Yersinia ... epithelial cells and the extracellular matrix proteins fibronectin and laminin [9], and Rck has recently also been reported to mediate a zipper-like internalization of S Enteritidis into cells in vitro ... producing potential of the intestinal flora of different hosts under varying conditions Using a RIVETmethod (Recombination-based In Vivo Expression Technology) to record SdiA activity in vivo,...

Ngày tải lên: 12/08/2014, 18:22

... benefits of genetic manipulation include controlling differentiation of ES cells, screening and acquiring pure populations of specific types of ES cell-derived cells, altering antigenicity of cells ... fluorescent protein ES cells FBS hES cells embryonic stem cells fetal bovine serum human embryonic stem cells HIV human immunodeficiency virus HSV herpes simplex virus ITR inverted terminal repeat ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...

Ngày tải lên: 11/09/2015, 14:38

... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... involving blastocyst injections and ectopic grafting in adult hosts cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains of ... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of...

Ngày tải lên: 16/09/2015, 15:55

... importance of Activin/ Nodal signaling in the maintenance of hESC pluripotency has been re-iterated in recent studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells ... Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of ... clusters of cells called Islets of Langerhans which perform its endocrine functions Acinar cells secrete digestive enzymes like trypsin and chymotrypsin into the small intestine Islets of Langerhans...

Ngày tải lên: 04/10/2015, 16:03

... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It ... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells ... achievement of iPS cells, by overexpressing four genes containing LIN28, reinforced the notion that LIN28 is a key regulator of pluripotency in human ES cells (Yu et al., 2007) 1.4.1 The interaction of...

Ngày tải lên: 16/10/2015, 11:58

... and Vimentin expressions in SDIA cultures List of Illustrations Illustration – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Introduction Mouse embryonic stem cells (mESC) ... life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells (mESC) a Culturing Conditions b Expression of Oct-3/4, Alkaline Phosphatase ... subsequent engineering of short, hairpin vectors under the control of Tet-R, have the advantage of being easily created for the specific gene of interest We selected the Invitrogen BlockIT Tet-inducible...

Ngày tải lên: 27/11/2015, 11:25

... at 37°C in a humidified atmosphere containing 5% CO2, while μg/mL doxorubicin was contained in the culture to maintain K562/AO2 cells drug resistance in its daily culture The inhibition of cell ... K562/AO2 cells than that of K562 cells, as shown in Figure 4a and 4b Interestingly, Page of the presence of PLA/Au nanocomposites could efficiently facilitate the drug uptake of DNR into the ... leukemia cells and lead to the much higher intracellular drug concentration in the target cells, resulting in the remarkable enhancement of the intracellular fluorescence of drug-resistant leukemia cells...

Ngày tải lên: 21/06/2014, 08:20

... than HePC in preclinical investigations [29,51] Intriguingly, in our in vitro study ErPC3 was a more effective inducer of apoptosis in T98G cells than ErPC when given alone and in combination with ... contributes to increased efficacy of the combination Increased induction of apoptosis contributes to increased efficacy of the combination (A, B) T98G cells were treated with a single dose of 10 Gy ... ability of T98G cells and increase radiation-induced eradication of clonogenic T98G cells Up to now our data indicated that ErPC and ErPC3 increase sensitivity of AC/GBM cell lines to radiation induced...

Ngày tải lên: 09/08/2014, 10:20

... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the ... purposes) Journal of Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected ... the saline- or hMNCs-injected groups, CBF in the hES-ECinjected group increased significantly, while no significant increase in the number of mouse PECAM-1 positive cells was observed in the...

Ngày tải lên: 18/06/2014, 15:20

... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or ... of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, fixed overnight in PBS-buffered 4%...

Ngày tải lên: 09/08/2014, 14:22

... fixed in 1% Formaldhyde in PBS for 15 minutes at room temperature Cells were rinsed with PBS twice and incubated in a 1/10000 dilution solution of DAPI (10 mg/ml) in PBS for 15-20 minutes Cells ... library in Genbank (Genbank ID: BG970953.1) with the same intron splicing pattern as the truncated form of MsrA cloned from embryonic stem cells, indicating this isoform might not be stem cell ... configuration of the subcellular localization of the truncated protein than a single view On the focal point of the scans (crosspoint of the horizontal green line and the vertical pink line), clearly...

Ngày tải lên: 10/08/2014, 05:21