... xeno-free cryopreservation method for the storage ofhumanembryonicstemcellsStemCells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... VS, Jones JM Embryonicstem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstem cell lines from human blastocysts: ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...
... expression pattern of undifferentiated and differentiated humanembryonicstemcellsStemCells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... 13 The expression of miR-92 has been reported in humanembryonicstem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... Mitchell PS, Nelson AM, et al.: MicroRNA Discovery and Profiling in HumanEmbryonicStemCells by Deep Sequencing of Small RNA Libraries StemCells 2008, 26(10):2496-2505 Seitz H, Royo H, Bortolin...
... embryonicstem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... stem cell; hESC, humanembryonicstem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; ... epigenomic profiling of a cohort ofhumanembryonicstem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual...
... specific diseases in the course of ex vivo gene therapy 1.1.1 HumanEmbryonicStemCellsHumanembryonicstem (hES) cells are cells derived from the inner cell mass ofhuman blastocysts The hES cell ... achieve desirable level of transient transgene expression in humanembryonicstemcells Efficient gene transfers to humanembryonicstemcells were obtained after optimization of the promoters and ... MODIFICATION OFHUMANEMBRYONICSTEMCELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...
... classes of pluripotent stemcells in humans, other primates and mice These are embryonicstem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... Adult stemcells Adult stemcells also known as somatic stemcells can be found in diverse tissues and organs The most common adult stemcells are the hematopoietic stem cells, mesechymal stemcells ... blood cells and platelets, while skin stemcells give rise to the various types of skin cells Some recent reports suggest that adult stemcells such as haemopoietic stem cells, neuronal stem cells...
... in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin ofhumanembryonicstemcells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure ... differentiation of hESCs ix LIST OF SYMBOLS α – Alpha β – Beta Δ – Delta δ – Delta μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonicstemcells The isolation of mouse and humanembryonicstem ... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) ofhumanembryonicstem cells...
... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonicstem and embryonic germ cells derived from discarded human embryos and germ line stemcells Adult stemcells can be derived ... 1.4 Stemcells in bone repair 13 1.5 HumanEmbryonicstemcells 15 1.5.1 Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells ... specific differentiation of hES cells is mandatory 1.5 HumanEmbryonicstemcells As the name suggests, the humanembryonicstemcells are derived from the early embryo These cells have the capacity...
... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... Figure cells differentiated from humanof the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated ... after ischemia Preparation ofhuman ECs and/or MCs derived from human ES cells Maintenance ofhuman ES cell line (HES-3) was described previously [10] We plated small human ES colonies on OP9 feeder...
... which include issues of tumorigenicity and functionality of ESCs 2.2 HumanEmbryonicStemCells (hESCs) Embryonicstemcells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... Hours - hr Human- specific vimentin - hVIM Humanembryonicstemcells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal cell line - HPM Human umbilical ... temporary relief of symptoms Stemcells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stemcells (MSCs) and embryonicstemcells (ESCs)...
... 2.7 Embryonicstemcells and induced pluripotent stemcellsEmbryonicstemcells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... the study of SHH signaling in hESC offers to augment the current understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Humanembryonicstemcells (hESC) ... conditioned media (CM) used for daily hESC culture feeding 3.2.3 Humanembryonicstemcells and induced pluripotent stemcellsHumanembryonicstem cell line HES-3 (46, XX) was from ES Cell International...
... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonicstemcells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin ... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... question of whether HS3-specific transcription is unique to the mouse embryonicstem cell system, we also analyzed transcription in the b-globin gene locus in human CD133+ hematopoietic progenitor cells, ...
... culture, expansion and manipulation ofhuman pluripotent stemcells The culmination of two events has enabled the production ofhumanembryonicstem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion ofhumanembryonicstem cells, and more recently, ofhuman induced pluripotent stemcells The ... translational research in the field ofembryonic and induced pluripotent stemcells It commences with practical aspects of the derivation and growth ofhumanembryonicstemcells from inner cell mass...
... Generation of functional hemangioblasts from humanembryonicstemcells Nat Methods 2007, 4(6):501-9 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation ofhumanembryonicstem ... Platt JL: Immunosuppression by embryonicstemcellsStemCells 2008, 26(1):89-98 Fabricius D, Bonde S, Zavazava N: Induction of stable mixed chimerism by embryonicstemcells requires functional Fas/ ... Characterization of the expression of MHC proteins in humanembryonicstemcells Proc Natl Acad Sci USA 2002, 99(15):9864-9 Li L, Baroja ML, Majumdar A, Chadwick K, Rouleau A, Gallacher L, et al.: Human embryonic...
... Culture and characterization ofhumanembryonicstemcellsStemCells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity ofhumanembryonic stem- derived cells Trends Biotechnol 2004, ... limitation of adult stemcells is their inability to effectively grow in Humanembryonicstemcells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stemcells ... culture hES cells on feeder-free conditions or human feeder cells for safe transplantation ofhuman ES cells The use of feeder-free systems, such as Matrigel or other components of the extracellualr...
... number ofcells was determined spectrophotometrically entiation ofhuman mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation ofhuman mesenchymal stem ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation ofhuman osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... cells/ cm2 ofhuman MSCs were seeded in BM in each well of 24-well plates (Becton Dickinson Labware, Franklin Lakes, NJ, USA) Each experimental condition was repeated in quadruplicate On day of...
... differentiation of cultured human mesenchymal stemcells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis ofhuman mesenchymal stem ... mesenchymal stemcells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation ofhuman adipose-derived adult stem ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...
... humanembryonicstemcells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from humanembryonicstemcells exhibit an embryonic ... hematopoietic differentiation ofhumanembryonicstemcells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Humanembryonicstem cell-derived NK cells acquire functional receptors ... H, Baker SC, et al.: Genome wide profiling ofhumanembryonicstemcells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC...