quantitative 2d imaging of human embryonic stem cells ý pdf

... xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...

Ngày tải lên: 31/10/2012, 16:57

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... Mitchell PS, Nelson AM, et al.: MicroRNA Discovery and Profiling in Human Embryonic Stem Cells by Deep Sequencing of Small RNA Libraries Stem Cells 2008, 26(10):2496-2505 Seitz H, Royo H, Bortolin...

Ngày tải lên: 18/06/2014, 15:20

... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; ... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual...

Ngày tải lên: 11/08/2014, 12:21

... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... achieve desirable level of transient transgene expression in human embryonic stem cells Efficient gene transfers to human embryonic stem cells were obtained after optimization of the promoters and ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...

Ngày tải lên: 11/09/2015, 14:38

... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells...

Ngày tải lên: 16/09/2015, 15:55

... in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure ... differentiation of hESCs ix LIST OF SYMBOLS α – Alpha β – Beta Δ – Delta δ – Delta μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem ... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem cells...

Ngày tải lên: 04/10/2015, 16:03

... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells can be derived ... 1.4 Stem cells in bone repair 13 1.5 Human Embryonic stem cells 15 1.5.1 Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells ... specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity...

Ngày tải lên: 09/10/2015, 11:24

... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells 1.1.4 Human...

Ngày tải lên: 16/10/2015, 11:58

... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated ... after ischemia Preparation of human ECs and/or MCs derived from human ES cells Maintenance of human ES cell line (HES-3) was described previously [10] We plated small human ES colonies on OP9 feeder...

Ngày tải lên: 18/06/2014, 15:20

... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal cell line - HPM Human umbilical ... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs)...

Ngày tải lên: 11/09/2015, 09:57

... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff ... types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells...

Ngày tải lên: 11/09/2015, 10:00

... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... the study of SHH signaling in hESC offers to augment the current understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Human embryonic stem cells (hESC) ... conditioned media (CM) used for daily hESC culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International...

Ngày tải lên: 11/09/2015, 10:14

... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin ... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... question of whether HS3-specific transcription is unique to the mouse embryonic stem cell system, we also analyzed transcription in the b-globin gene locus in human CD133+ hematopoietic progenitor cells, ...

Ngày tải lên: 07/03/2014, 12:20

... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... Generation of functional hemangioblasts from human embryonic stem cells Nat Methods 2007, 4(6):501-9 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem ... Platt JL: Immunosuppression by embryonic stem cells Stem Cells 2008, 26(1):89-98 Fabricius D, Bonde S, Zavazava N: Induction of stable mixed chimerism by embryonic stem cells requires functional Fas/ ... Characterization of the expression of MHC proteins in human embryonic stem cells Proc Natl Acad Sci USA 2002, 99(15):9864-9 Li L, Baroja ML, Majumdar A, Chadwick K, Rouleau A, Gallacher L, et al.: Human embryonic...

Ngày tải lên: 18/06/2014, 15:20

... Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... limitation of adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells ... culture hES cells on feeder-free conditions or human feeder cells for safe transplantation of human ES cells The use of feeder-free systems, such as Matrigel or other components of the extracellualr...

Ngày tải lên: 07/08/2014, 18:21

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... cells/ cm2 of human MSCs were seeded in BM in each well of 24-well plates (Becton Dickinson Labware, Franklin Lakes, NJ, USA) Each experimental condition was repeated in quadruplicate On day of...

Ngày tải lên: 09/08/2014, 10:23

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose-derived adult stem ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...

Ngày tải lên: 09/08/2014, 14:22

... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J: Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH, Ho LL, Fu ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells...

Ngày tải lên: 10/08/2014, 05:21

... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell-derived NK cells acquire functional receptors ... H, Baker SC, et al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC...

Ngày tải lên: 14/08/2014, 08:20