high pressure liquid chromatography hplc in food authentication

Ngày tải lên: 09/03/2014, 18:55

... Glucine; SER: Serine; GLY: Glycine ; HIS: Histidine ARG: Arginine; THR: Threonine; ALA :Alanine; PRO: Proline; TYR: Tyrosine ; VAL: Valine ; MET: Methionine; CYS: Cysteine; ILEU: Isoleucine; ... Arginine; THR: Threonine; ALA :Alanine; PRO: Proline; TYR: Tyrosine ; VAL: Valine ; MET: Methionine; CYS: Cysteine; ILEU: Isoleucine; LEU: Leucine; PHE : Phenylalanine; LYS: lysine Hình 4.10 Sắc ... Arginine; THR: Threonine; ALA :Alanine; PRO: Proline; TYR: Tyrosine ; VAL: Valine ; MET: Methionine; CYS: Cysteine; ILEU: Isoleucine; LEU: Leucine; PHE : Phenylalanine; LYS: lysine Theo bảng này,...

Ngày tải lên: 28/07/2014, 06:20

... amino acid Pleurotus sp sắc ký giấy tròn (Zakia Bano, 1962) L: lycine IL:Isoleucine Ph Al: Phenylalanine Glu: Glutamic acid Thr: Threonine Ser: Serine Gly: Glycine Asp: Aspartic acid Arg: Agrinine, ... Thr: Threonine Ser: Serine Gly: Glycine Asp: Aspartic acid Arg: Agrinine, Pep: Peptide Lys:Lysine, His: Histidine Cys: Cysteine 23 PHẦN PHƢƠNG PHÁP NGHIÊN CỨU 3.1.Thời gian, địa điểm thực vật ... điểm Trung tâm Công Nghệ Sinh Học, Đại học Nông Lâm Thành phố Hồ Chí Minh Phòng Hóa Lý, Trung tâm Phân Tích Thí nghiệm Hóa Sinh, Đại học Nông Lâm Thành phố Hồ Chí Minh 3.1.3 Vật liệu nghiên cứu...

Ngày tải lên: 28/07/2014, 06:20

... phân tích amino acid Phân tích amino acid phƣơng pháp xác định thành phần amino acid nhƣ định lƣợng chúng mẫu protein hay peptide Phân tích amino acid sử dụng sắc ký giấy (paper chromatography) , ... sắc ký lớp mỏng (thin layer chomatographyTLC), sắc ký khí (gas chromatography) , sắc ký trao đổi ion (ion exchange chromatography) , sắc ký lỏng cao áp (high performance liquid chromatography) , điện ... phát thông qua UV (Ultraviolet) 2.4.3 High Performance Liquid Chromatography (HPLC) HPLC đƣợc đời vào đầu năm1970 công cụ phân tích đầu tay nhà phân tích HPLC dựa vào pha động (dạng lỏng) để phân...

Ngày tải lên: 28/07/2014, 06:20

... phân tich amino acid 13 iv 2.4.1 Sắc ký giấy (Paper chromatography) 13 2.4.2 Sắc ký lớp mỏng ( Thin layer chromatography _TLC) 14 2.4.3 High Performance Liquid Chromatography (HPLC) ... phần dinh dƣỡng chúng, đặc biệt thành phần amino acid đƣợc quan tâm Để thực phân tích amino acid nấm, tiến hành trồng nấm Trung tâm Công Nghệ Sinh Học sử dụng thiết bị sắc ký lỏng cao áp (HPLC) ... SẮC KÝ LỎNG CAO ÁP (High performance liquid chromatography - HPLC) Giáo viên hƣớng dẫn: Sinh viên thực hiện: TS LÊ ĐÌNH ĐÔN ĐOÀN BẢO QUỐC Thành phố Hồ Chí Minh Tháng 9/2005 LỜI CẢM TẠ CHÂN THÀNH...

Ngày tải lên: 28/07/2014, 06:20

... cytosine, cytidine, adenosine, adenine, inosine, guanine, cordycepin, uracil, and uridine (>99%; Sigma) were available for the identification of compounds in the samples Sample preparation and chromatography ... nucleosides or nucleobases (namely, cytidine, cytosine, adenine, adenosine, uridine, uracil, guanine, inosine, guanosine, and 2’-deoxyadenosine) were identified in the mycelia AC (Figure 1) Based on ... compounds in water and methanol extracts of Antrodia camphorata: 1, cytosine; 2, uracil; 3, guanine; 4, cytidine; 5, uridine; 6, adenine; 7, inosine; 8, guanosine; 9, adenosine; 10, 2’-deoxyadenosine;...

Ngày tải lên: 13/08/2014, 15:21

... determination of sulfonamide residues in meat Journal of Chromatography A 1159 (2007)233-241 SUMMARY DETERMINATION SULPHONAMIDES RESIDUES IN FOOD BY HIGH PERFOPvMANCE LIQUID CHROMATOGRAPHY PART SEPARATION ... 4,5 Can chinh xac mdt lugng muoi CH3COONa.H20 loai tinh khiet phan tich da tinh trudc, boa tan bing khoang 900 ml nudc cit hai lan, chinh pH ciia dung djch bing axit axetic 20% loai tinh khiet ... Results was indicated sulphonamides residues in some samples of animal foodstuffs and food products higher than maxium residue level [2] Dia chi: Nhdn bdi ngdy 29 thdng ndm 2008 Chu Dinh Binh, Nguyen...

Ngày tải lên: 20/03/2014, 21:20

... dựng tiêu chuẩn Phần II: Hệ thống HPLC L ưu ý : - T ất c ả dung môi dùn g cho HPLC đề u ph ải dung môi tinh ết có ghi rõ nhãn dùn g cho HPLC Hay dung môi tinh ết phân tíc h - T ất c ả hóa ch ... m ềm nh ận,tín hi ệu s l ý d ữ li ệu ều ển h ệ th ống HPLC 8- In d ữ li ệu Phần II: Hệ thống HPLC II.1 - Bình đựng dung môi - Hiện máy HPLC thường có 04 đường dung môi vào đầu bơm cao áp Cho ... ắc k ý h ấp ph ụ ( NP - HPLC va RP - HPLC) ; s ắc k ý phân b ố - s ắ c k ý chi ết ( LLC); s ắc k ý trao đổi ion ( IE - HPLC) ; s ắ k ý rây c phân t - s ắc k ý gel ( IG - HPLC) Nh th ự t ế hi ện...

Ngày tải lên: 22/03/2014, 18:20

... HPLC Zhongguo Xinyao Yu Lingchuang Yaoli 2006, 17:359-361 Yang WL, Yang SL, Zhang M, Huang JL: Determination of naringin and neohesperidin in fructus aurantii by RP -HPLC Zhongguo Xinyao Yu Lingchuang ... isonaringin (B), neohesperidin (C) and hesperidin (D) Page of 10 (page number not for citation purposes) Chinese Medicine 2009, 4:15 Nanjing, China Prof Ping Li at the Key Laboratory of Modern Chinese ... fragmentation of naringin and isonaringin based on quasi-MSn (up to MS4) spectra in negative ion mode Proposed fragmentation of naringin and isonaringin based on quasi-MSn (up to MS4) spectra in negative...

Ngày tải lên: 13/08/2014, 15:21

... constituents contained in samples Sample Plant sources Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Wine Wine Wine Beverage ... peak-tailing, the effects of several additives and their concentration were investigated In liquid chromatography, triethylamine was the most common additive used in analyzing compounds containing ... containing 20 mmol/l ammonium acetate and 0.2% formic acid (v/v) The gradient elution was programmed as follows: A was maintained at 35% within the first 10 min, then linearly increased to 80% during...

Ngày tải lên: 02/09/2015, 13:06

... Histamine poisoning occurs after consuming seafoods containing high level of either histamine or other biogenic amines This foodborne intoxication was originally called “scombroid poisoning” because ... determine histamine in seafoods by high performance liquid chromatography - Studying the development of histamine in seafood over time and storage temperature - Quantification of histamine content ... flow injection analysis, and high performance liquid chromatography (HPLC) Histamine has a very important role in the food industry In recent years, many studies appear to study on histamine due...

Ngày tải lên: 06/10/2015, 13:05

... of the proteins in each HPLC peak The peaks eluting within 25 represented linker proteins, while the four main peaks between 28 and 33 were the phycocyanins and allophycocyanins [15] In Table the ... phycocyanin to phycoerythrin in spectroscopy of biliproteins In uence of aggregation and linker rods of certain phycobilisomes to improve light harvesting proteins on chromophore structure in allophycocianin ... stained with Coomassie Brilliant Blue R-250 dissolved in acetic acid: methanol: water 10 : 40 : 50 (v/v/v) High performance liquid chromatography Protein separation by HPLC was performed using...

Ngày tải lên: 08/03/2014, 16:20

... In recent years, high performance liquid chromatography (HPLC) and gas chromatography (GC) have been employed to determine the level of anthraquinones in Radix Polygoni Multiflori [32,33] Using ... acetonitrile (containing 0.5% acetic acid)/water (containing 0.5% acetic acid) at a flow rate of mL/min by a discontinuous gradient in which acetonitrile was adjusted Chemicals and reagents HPLC- grade ... 2,3,5,4′-tetrahydroxystilbene-2-O-b-Dglucopyranoside (THSG), emodin and physcion Figure HPLC fingerprints of commercial decoction pieces of Radix Polygoni Multiflori from Deqing County, Guangdong, China Liang et al Chinese Medicine 2010, 5:29...

Ngày tải lên: 13/08/2014, 15:21

... solution at the beginning indicating that P420 was not formed during the high- pressure stopped-flow experiment The high- pressure stopped-flow apparatus used is interfaced with the Aminco DW2 spectrometer ... and slow CO binding kinetics (1R-camphor and camphane) (b) There are two complexes which show two-phase CO binding kinetics (substrate-free, camphane) In the following both these findings will be ... present) In conclusion, CO binding in camphor-bound P450cam is statistically disfavoured and therefore slow Ó FEBS 2002 High- pressure stopped-flow for P450 CO binding (Eur J Biochem 269) 2995 Extending...

Ngày tải lên: 08/03/2014, 23:20

... performed in ammonium acetate buffer 10 mM Thus, calibration curves were obtained by spiking increased amounts of analytes in mM ammoniumacetate and in pooled EBC All calibration curves obtained in ... is mainly formed by water vapour which contains non-volatile compounds in the aerosol particles carried away during breathing Pooled EBC was used as representative matrix for individual gained ... possible to show a higher correlation applying each inter- Results and discussion Optimization of enrichment and chromatography Increasing the fraction of solvent B as shown in Table the analytes...

Ngày tải lên: 20/06/2014, 00:20

... roxithromycin in various tissues obtained at different time points after treatment of roxithromcyin mixed with drinking water (15 mg/L) for days Mean ± S.D (㎍/g) Tissues Liver Kidney Small intestine Skin ... Quantitative determination of the macrolide antibiotics erythromycin, roxithromycin, azithromycin and clarithromycin in human serum by high- performance liquid chromatography using pre-column derivatization ... Determination of Roxithromycin by Liquid Chromatography/ Mass Spectrometry after Multiple-Dose Oral Administration in Broilers 39 Ta ble Concentration of roxithromycin in various tissues obtained...

Ngày tải lên: 07/08/2014, 17:22

... were measured by connecting the flowmeter to a shoot and perfusing water at 0.2 MPa pressure for or h Initially, flow rate was high but declined gradually The initially high flow rate was attributed ... constructed from glass tubing, tygon tubing, stopcocks, and plastic T-junctions Water was held in a flexible plastic bag inside a pressure reservoir (R) Water contained in the reservoir was distilled ... to differ- ent values measuring by changing the air pressure in R and the rate of flow (kg s into the con) -1 tainer of water on the balance Calibration curves were linear with a maximum deviation...

Ngày tải lên: 08/08/2014, 23:22

Ngày tải lên: 20/10/2014, 17:16