haemophilus bordetella and legionella

... 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshly prepared and used immediately Effect of various compounds on the enzyme activity The effect of metal salts, and chelating and sulfhydryl ... of Bordetella hinzii DSM 4922T (AF177667), and 96.0% identical with that of Bordetella bronchiseptica (AJ278452) [26,27] This close phylogenic relatedness with other members of the genus Bordetella ... 4-amino-3-hydroxybenzoic acid, 6.0 g Na2HPO4Æ12H2O, and deionized water in 700 mL total volume; the final pH being adjusted to pH 6.8 Solutions A and B were autoclaved, and solution C was sterilized by filtration...

Ngày tải lên: 21/02/2014, 01:21

... In this study we describe the identification and structural analysis of a Sial-lNnT unit in H influenzae LPS MATERIALS AND METHODS Bacterial strains and culture conditions The H influenzae strain ... ms, and 20480 transients for a duration of 21 h Electrophoretic analysis of LPS LPS was prepared and analysed by tricine-SDS/PAGE as described previously [10] Ó FEBS 2002 Sialylated LPS in Haemophilus ... specifically cleaves sialic acid residues (Fig 1; lanes and 2) H influenzae LPS typically migrates as a complex series of bands in SDS/PAGE, each band corresponding to gain or loss of a glycose moiety...

Ngày tải lên: 23/03/2014, 21:21

... Kdo Fig Binding curves of mAbs S42-16 (A and B), S42-21 (C and D), and S42-10-8 (E and F) to Kdo4P-GlcN2P2-BSA (A, C and E) and Kdo5P-GlcN2P2-BSA (B, D and F) ELISA plates were coated with 200 ... ligands and as neoglycoconjugates now enables us to investigate further this antibody by NMR and crystallography ACKNOWLEDGEMENTS We thank R Moxon (Oxford, UK) for strain I-69 and V Susott and ... with glutardialdehyde and conjugated to BSA as described [12] Chemical analyses indicated a molar ratio of protein to ligand of : 1.1 and : 1.0 for Kdo-4P-GlcN2-P2-BSA and Kdo-5P-GlcN2P2-BSA,...

Ngày tải lên: 31/03/2014, 21:21

... Bordetellae B pertussis: ho gà, viêm tai  B parapertussis: ho gà (5%)  B bronchiseptica  B avium  B hinzii  B holmesii  B trematum  Bordetella pertussis (VI KHUẨN ... Erythromycin, sulfamethoxazole-trimethroprime - γ - globulin - Corticoide - Thở không khí lành Haemophilus influenzae Haemophilus influenzae Do Richard Pfeiffer phân lập lần từ bệnh nhân bò chết vụ dòch ... Năm 1917, Winslow cộng sự, sở hiểu biết B influenzae, chuyển chúng sang giống Haemophilus, từ đến nay, chúng mang tên Haemophilus influenzae  H influenzae ĐẶC ĐIỂM VI SINH HỌC: Hình dạng: - Gr...

Ngày tải lên: 28/05/2014, 21:59

... TRỰC KHUẨN HO GÀ ( Bordetella pertussis)  MỤC TIÊU Các đặc điểm sinh học TKHG Cơ chế gây bệnh Ho gà Các phương pháp VSV chẩn đoán bệnh Ho gà Các nguyên tắc phòng chữa bệnh Ho gà Bordetella pertussis ... Chăm sóc cẩn thận, dd, dịch… - KS chon lọc: Erythromycin (ít tuần) Haemophilus influenzae  MỤC TIÊU Đặc tính khuyết dưỡng Haemophilus influenzae Các đặc điểm sinh học (hình thể, t.c nuôi cấy, ... điều trị Haemophilus influenzae Tính khuyết dưỡng - Không tự tổng hợp số yếu tố cần thiết để sinh sản, phát triển - Yếu tố X: hematin - Yếu tố V: NAD (nicotinamid adenin dinucleotid)  Haemophilus...

Ngày tải lên: 11/06/2014, 09:51

... assay Antibody titers in egg yolks and sera were measured by ELISA OMPs and DNT from B bronchiseptica and P multocida D:4, OMPs from A pleuropneumoniae serotypes and and IROMPs from P multocida A:3 ... pleuropneumoniae serotypes and (Fig 6) Immune responses to A pleuropneumoniae serotypes and Immune responses to A pleuropneumoniae serotypes and in egg yolks and sera steadily increased, and were maintained ... Chae, H S., and Sim, J S Effects of egg and yolk weights on yolk antibodies (IgY) production in laying chickens Poultry Sci 1998, 77, 266-270 Marandi, M V and Mittal, K R Identification and characterization...

Ngày tải lên: 07/08/2014, 15:20

... compared to medium (Fig and 3) Fig Serum-mediated B bronchiseptica binding and uptake by AM Binding (A, B) and uptake (C, D) assays were performed as described in Materials and Methods using various ... were lysed and the number of viable intracellular bacteria (CFU) was determined using blood agar plate as described in Materials and Methods Mac-1-mediated Uptake and Killing of Bordetella bronchiseptica ... Goodnow , R A Biology of Bordetella bronchiseptica Microbiol Rev 1980, 44, 722-738 Guzman, C A., Rohde, M., Bock, M and Timmis, K N Invasion and intracellular survival of Bordetella bronchiseptica...

Ngày tải lên: 07/08/2014, 17:22

... acitpesihcnorb B EGFP rof AND fo noitaraperP setalosi eht fo smargordned tcurtsnoc ot desu osla saw hcihw ,)ecnarF ,tamruoL rebliV( erawtfos D2 oiB liforP-oiB gnisu dezylana erew sdnab AND eht dna )ecnarF ... ni )napaJ ,arakaT( sPTNd Mm 005 dna ,)ASU ,agemorP( esaremylop AND qaToG fo stinu 5.2 ,remirp elomp 21 ,)ASU ,agemorP( 2lCgM Mm ,AND latot fo gn 05 fo detsisnoc RCP acitpesihcnorb B DPAR sniarts ... hcae morf AND cimoneG h 42 rof Co73 ta detabucni dna )ASU ,ocfiD( htorb noisufni traeh niarb hserf lm otni detaluconi erew setalosi lairetcaB acitpesihcnorb B DPAR rof noitaraperp AND desu litnu...

Ngày tải lên: 07/08/2014, 20:23

... by a retroperitoneal abscess caused by Haemophilus influenzae and Haemophilus parainfluenza Gastroint Endosc 1998, 47:417-418 Laing RBS, Leen CLS, Watt B: Haemophilus parainfluenza: an unusual ... carried out the search and obtained data on H parainfluenza, and cross referenced its occurrence to extra-respiratory infections, assisted in compiling the manuscript, and also carried out chart ... carried out the search and obtained data on H parainfluenza, and cross referenced its occurrence to extra-respiratory infections, assisted in compiling the manuscript, and also carried out chart...

Ngày tải lên: 11/08/2014, 12:20

... experiments KR and SO performed the ELISA AJU and KR did the cell culture and BAL analyses SM and MA did the IHC and RT-PCR HS and EV took the pathologic part of the study PZ and KD were involved ... upregulation of BAMBI in the lung tissue on AM and AEC as well as on isolated AM and A549 cells This was demonstrated on RNA and protein level (figure [5band 5d] and 6[a-d]) This induction was observed ... demonstrated in figure 4c and 4d Inhibition of p38 significantly inhibited CXCL-8 and TNF-α expression (p < 0.01) NTHI infection of lung tissues (NTHI-1 and NTHI-2) and A549 cells generated a...

Ngày tải lên: 12/08/2014, 11:22

... extracellular and intracellular receptors and contribute to host innate immunity [3-5] Lipid metabolites of arachidonic acid such as prostaglandins have been shown to modulate immune and inflammatory ... vivo mice experiments Mice experiments C57BL/6 and BALB/c mouse strains, background strain for TLR2 and TLR4 knock-out, respectively, and TLR2 and TLR4 knock-out mice were used for NTHi-induced ... (NCI, NIH, USA) and TLR2 knock-out mice were kindly provide by Dr S Akira, and TLR4 knock-out mice were purchased from Jackson Lab (USA) Under the anesthesia, wild-type and TLR2 and TLR4 knock-out...

Ngày tải lên: 12/08/2014, 15:21

... experiments and was involved in the design and coordination of the study and drafting the manuscript FH measured HMGB1 levels, and was involved in the design and coordination of the study and drafting ... SH, SY, MK and MT were involved in the design and coordination of the study HT and NM were involved in western blot analyses JF was involved in the design and coordination of the study and drafting ... humidified incubator, and subsequently subcultured in buffered yeast extract broth supplemented with α-ketoglutarate (BYE-α) Legionella pneumonia is a potentially serious and lifethreatening pneumonia...

Ngày tải lên: 12/08/2014, 15:21

... individual genome using a random shotgun library approach Lander-Waterman statistics provide an answer in the latter case by using the assumption that reads are independently and randomly sampled from ... Global genomic alignments of PittEE against R2846 and R2866 were performed (Figures 10 and 11) PittEE and R2846 are very similar at the global level and this is reinforced by the gene cluster analysis, ... distributed and unique genes, and as a consequence, these non-core genes are more likely to have foreign origins Interestingly, there is no significant difference between distributed and unique genes and...

Ngày tải lên: 14/08/2014, 07:21

... 5.8 Anti-pertussis (A, B and C) and anti-M2e (D, E, and F) local and systemic immune responses in mice immunized with BPST6 strains 153 Figure 5.9 Survival rate (A) and average body weight ... double-stranded RNA by TLR3 and single-stranded RNA by TLR7 and TLR8 has also been proposed (Diebold et al., 2004; Lund et al., 2004) TLR3 is expressed by human lung epithelial cells, and activation ... MORTALITY AND HISTORY OF INFLUENZA PANDEMICS 2.2 INFLUENZA VIRUSES CLASSIFICATION 2.3 INFLUENZA A VIRUS: STRUCTURE AND REPLICATION 2.3.1 Influenza A Virus Genome and Its...

Ngày tải lên: 11/09/2015, 10:00

... Bordetellae        B pertussis: ho gà, viêm tai B parapertussis: ho gà (5%) B bronchiseptica B avium B hinzii B holmesii B trematum Bordetella pertussis (VI KHUẨN ... Erythromycin, sulfamethoxazole-trimethroprime - γ - globulin - Corticoide - Thở không khí lành Haemophilus influenzae Haemophilus influenzae Do Richard Pfeiffer phân lập lần từ bệnh nhân bò chết vụ dòch ... Năm 1917, Winslow cộng sự, sở hiểu biết B.influenzae, chuyển chúng sang giống Haemophilus, từ đến nay, chúng mang tên Haemophilus influenzae  H influenzae ĐẶC ĐIỂM VI SINH HỌC: Hình dạng: - Gr...

Ngày tải lên: 11/09/2015, 19:04

... Chapter 2: Literature Review 2.1 Legionella 2.1.1 Introduction to Legionella 2.1.2 General characteristics of Legionella 2.1.3 Taxonomy of Legionella 2.1.4 Legionella and Diseases 2.1.4.1 Clinical ... Ecology of Legionella 16 2.1.5.1 Natural and man-made habitats 16 2.1.5.2 Distribution of Legionella in Singapore 18 2.1.5.3 Association of Legionella with protozoa 19 2.1.5.4 Association of Legionella ... structure and porosity, it was determined if there is any difference (in numbers and distribution pattern) in accumulation and persistence of L pneumophila in developing and mature biofilm, and if...

Ngày tải lên: 08/11/2015, 16:31

... Ⅲ Understand the embedded Linux development environment Ⅲ Understand and create Linux BSP for a hardware platform Ⅲ Understand the Linux model for embedded storage and write drivers and applications ... memory leaks and memory corruption in applications and drivers Ⅲ Learn methods to profile applications and the kernel Ⅲ Understand uCLinux architecture and its programming model Ⅲ Understand the embedded ... Ltd Ⅲ Windows, WinCE and Microsoft are registered trademarks and MS-DOS and DirectX are trademarks of Microsoft Corporation Ⅲ Solaris and Java are registered trademarks and ChorusOS is a trademark...

Ngày tải lên: 04/08/2012, 14:23

... during transport and handling – particle and ingredient segregation (separation) occurs when particles of different sizes and bulk densities are blended together and transported or handled Pellet ... Size, Bulk Density and pH Particle size and particle size uniformity of feed ingredients are important considerations of livestock and poultry nutritionists when selecting sources and determining ... Characteristics of U.S DDGS Physical and chemical characteristics of distiller’s dried grains with solubles (DDGS) vary among sources and can influence its feeding value and handling characteristics These...

Ngày tải lên: 08/08/2012, 10:03

Ngày tải lên: 08/08/2012, 17:20

... our products and how they work, how the distribution system operates, how to deal with both suppliers and customers and how to handle complaints They also study trade and retail laws, and are accompanied ... idioms IT and e-commerce Jobs and positions Letters Meetings and presentations Money and financial issues Numbers and symbols Phrasal verbs Phrasal verbs Production and operations Recruitment 1: ... part of a , and should have 10 and the ability to 11 and inspire his or her 12 Sandra: Well, that's great! You've always got on with the people you work with, and everyone...

Ngày tải lên: 08/08/2012, 17:31