goat apos s cheese fatty acid composition

... 2005) RESULTS AND DISCUSSION 3.1 Fatty acid compositions of instant noodles Table shows fatty acid composition of instant noodles analyzed Palmitic acid was the most abundant fatty acid in all samples; ... Fatty acid composition including trans fatty acids content of selected Vietnamese instant noodles Table1 Fatty acids composition of selected Vietnamese Instant noodles Fatty acid C14:0 Brands ... SD for three samples of triplicates * Fatty acids less than 0.1%: C15:0, C17:0, C21:1 * SFA: saturated fatty acids * PUFA: polyunsaturated fatty acids * TFA: trans fatty acids * MUFA: monounsaturated...

Ngày tải lên: 28/08/2013, 16:28

... way analysis of variance) as assessed by SDS ⁄ PAGE [optical density unitsÆ(lmol TG))1 (n ¼ 3): palm CRLPs, 102 ± 25; olive CRLPs, 122 ± 46; corn CRLPs, 135 ± 19; fish CRLPs, 99 ± 36] Assessment ... different types of CRLP was assessed using SDS ⁄ PAGE The gels were stained with Coomassie blue and the bands were quantified by absorbance volume analysis Cell protein content was measured by the ... remnants decreases their uptake by the cells It is possible therefore that the differential rates of uptake of CRLPs of different fatty acid compositions by macrophages observed in the present study...

Ngày tải lên: 16/03/2014, 12:20

... P < 0.01; olive CRLPs versus corn and fish CRLPs, P < 0.001 versus fish CRLPs, P < 0.01; olive CRLPs versus corn and fish CRLPs, P < 0.001) Discussion Although there is now substantial evidence to ... of three separate experiments Error bars show the SEM The efflux curves were significantly different (two-way ANOVA) as follows: palm CRLPs versus corn CRLPs, P < 0.001, versus fish CRLPs, P < 0.01; ... types of CRLPs Effect of the fatty acid composition of CRLPs on COX-2 mRNA expression The effect of CRLPs of varying fatty acid composition on expression of COX-2, an NF-jB target gene [40] was...

Ngày tải lên: 23/03/2014, 04:20

... sequencing and such sequences are known as expressed sequence tags (ESTs) The usefulness of ESTs as markers has been demonstrated in several plant species [16,17] ESTs help to map known genes apart from ... additional SSRs from the existing ESTs collection is in progress, and it is anticipated that the EST-SSRs will assist with map saturation in the future The proportion of markers exhibiting distorted segregation ... double asterisk: skewed marker at P < 0.05; three asterisks: skewed marker at P < 0.01; four asterisks: skewed marker at P < 0.005; five asterisks: skewed marker at P < 0.001; six asterisks: skewed...

Ngày tải lên: 12/08/2014, 03:21

... one fruit Small amounts of arachidic, cis- gac mesocarpb trans lycopene SDc RSDd cis-lycopene isomers SD RSD trans -carotene SD RSD cis -carotene SD RSD R-carotene SD RSD % total fresh wt gac ... Two whole fruits were analyzed in this way Most of the fruit is composed of mesocarp and seeds with their surrounding oily pulp (aril) Of these tissues, the mesocarp represents almost half of the ... (Agilent Technologies) Split injection was applied, and the same type of column and temperature program as described above was used Comparison to mass spectra of known FAMEs was used to identify...

Ngày tải lên: 09/02/2014, 00:08

... (northwestern Mediterranean) assessed by carbon and nitrogen stable isotope analysis, Estuar Coast Shelf Sci., 73, 299-315 Hemminga M A and Mateo M A (1996) Stable carbon isotopes in seagrasses, variability ... the fatty acid characteristics, some fatty acids are used as biomarkers to primary producers Table shows the biomarker fatty acid of primary producers in tidal flat by previous researches According ... CONCLUSIONS This study focused on the carbon stable isotope and fatty acid structure of Z japonica because stable isotope and fatty acids are useful methods to clarify the carbon cycle in the ecosystem,...

Ngày tải lên: 05/09/2013, 10:17

... 2-inch as diameter filter paper was used to collect smoke samples from the engine, through smoke sampling pump for measuring Bosch Smoke Number Results and discussions 3.1 Properties of biodiesel ... elevated loads It was found from the investigation that the smoke intensity for biodiesel fuels decreases with increase in percentage of unsaturation It is necessary to discuss the mechanism of smoke ... high saturated fatty acid composition More unsaturated biodiesel fuels emits lower HC, CO and Smoke emissions compared to highly saturated biodiesel fuel as More NOX was observed in case of...

Ngày tải lên: 05/09/2013, 15:28

... cellular steatosis as such is not responsible 522 for lipotoxicity Rather, it is likely that diversion of fatty acids into inert TAG stores contributes to cell survival and preserves cellular ... toxic effects induced by thapsigargin or tunicamycin (two classical ER stress inducers), indicating that oleate is not a general inhibitor of the ER stress response (results not shown) Stearate ... acid, 18:2 cis) or saturated (palmitate) fatty acids were used instead of HepG2 cells, oleate, and stearate, respectively (results not shown) Stearate treatment induces ER stress and mitochondrial-mediated...

Ngày tải lên: 14/02/2014, 22:20

... in our assay Discussion The conserved cation-binding site A possible cation site was discovered close to the active site This binding site is unaffected by the H298 mutations and is conserved ... M-R-G -S, a His tag of six residues followed by G -S Three assays were carried out on the purified enzymes (a) The acyl transferase assay measures the ability of a KAS protein to accept a fatty acid substrate ... various enzymes carry out synthesis of rather complicated molecules such as ring systems [13] and wax components [14] are attractive 696 properties for drug synthesis research The fatty acid condensing...

Ngày tải lên: 19/02/2014, 08:20

... disulphide adduct structures by GCMS as previously described [18] Sphingoid base analysis Sphingolipid analysis of yeast cells was carried out essentially as described previously [21] LCBs were liberated ... b5 fusion candidate desaturases (TpdesA, TpdesB, TpdesI, TpdesK and TpdesO) functioned as sphingolipid desaturases, LCBs were extracted from S cerevisiae cells after galactose-induced expression ... D8-desaturase, TpDESB This is the first example of a sphingolipid long chain base D8-desaturase from a marine algal species It has previously been observed that this class of sphingolipid desaturase displays...

Ngày tải lên: 20/02/2014, 01:20

... Q334A/K335A Q334H Q334H/K335A Q33 4S S53A/Q334H G56A/Q33 4S G5 6S/ Q334H G5 6S/ Q334H/K335A S/ G/Q S/ G/Q A/G/Q A/G/Q S/ A/Q S/ S/Q S/ S/Q S/ G/A S/ G/A S/ G/H S/ G/H S/ G /S A/G/H S/ A /S S /S/ H S/ S/H 87.1 ^ 22.2 (20) 114.9 ... variants towards uPA The most common amino -acid composition of the buried polar cluster (positions 53/56/334) in serpins is S/ S/H The composition S/ S/Q is identical to that of alaserpin, S/ G/H is ... serpins showed that residue 53 is a Ser in 92% of the cases; residue 56 is a Ser in 74% of the cases; residue 186 an Asn in 87% of the cases; and residue 334 a His in 80% of the cases [3] In...

Ngày tải lên: 22/02/2014, 07:20

... Wodak, S. J., Lauwereys, M., Stanssens, P., Mrabet, N.T., Snauwaert, J., Matthyssens, G & Lambeir, A.-M (1992) Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis Crystallography ... regard This heterogeneity among class II XIs may arise from the existence of additional salt bridge (s) specific to thermostable class II XIs [30] In a previous study of class II XIs, a positive correlation ... is available [10,17 –24] On the basis of the absence or presence of a 50-residue insert at the N-terminus, XIs have been classified into class I and class II enzymes, respectively [25] Two distinct...

Ngày tải lên: 22/02/2014, 07:20

... CA, USA) Unless otherwise stated, all chemical reagents were from Sigma-Aldrich FAS activity assay FAS activity was assayed by a spectrophotometric method using a Synergy Microplate Reader System ... slope of the progress curves during the first FAS inhibition studies Synthetic peptides and the C75 positive control compound were used for FAS inhibition studies with stock solutions of the synthetic ... the fatty acid reaches 16–18 carbon atoms in length, it is released by the thioestherase (TE) domain [1] Human FAS is downregulated in most normal human tissues but is highly expressed in adipose...

Ngày tải lên: 06/03/2014, 09:22

... FEBS M Scharnewski et al Fatty acid secretion in mutants of yeast A A B B C C Fig Profile of free and esterified fatty acids upon expression of D12-desaturase in MS51 Cells of strain MS51 expressing ... developed describing fatty acid movement across a membrane as a passive process This mechanism of simple diffusion is based on the fast flip-flop of fatty acids through the membrane and has been reviewed ... cells are able to deposit excess of free fatty acids specifically in the ER, thereby excluding an excess of free fatty acids from other cellular compartments Discussion The present study was designed...

Ngày tải lên: 07/03/2014, 05:20

... (chromosome) assignment, the conserved gene synteny with mammalian orthologs, and the tissue-specific distribution of fabp6 gene transcripts in embryos, larvae and adults Results and Discussion ... remains limited or, in many cases, unknown However, sufficient evidence exists to strongly suggest 3326 the following roles for FABPs: (a) uptake of fatty acids across the plasma membrane and transport ... phylogenetic analysis (Fig 3) strongly suggests that the ESTs and genomic sequence retrieved from DNA assembly Zv7, scaffold 296.3 (Wellcome Trust Sanger Institute zebrafish genome sequence) described above,...

Ngày tải lên: 07/03/2014, 06:20

... the desaturases involved in PUFA biosynthesis in other algae or protozoa is still lacking The pathway involving D8 desaturases was proposed to be present in some algae, such as Isochrysis sp., ... here As discussed previously, Isochrysis sp D4 desaturase appears to have evolved independently, probably from a D5 desaturase, conserving the consensus motifs for the three histidine boxes characteristic ... bloodstream forms and in L major promastigotes, and a role of prostaglandins in the pathogenesis of the diseases was suggested An additional function for C22 FAs could be assigned as trypanosomes...

Ngày tải lên: 07/03/2014, 12:20

... free cysteines Therefore E coli CFAS contains three classes of free cysteines, three fast reacting thiols (exposed), two slowly reacting thiols (less accessible) and three buried cysteines that ... min), the supernatent was loaded directly on a nickel CFAS assay CFAS activity was assayed as described previously [7] with slight modications The assay consisted in 1.0 mgặmL)1 phopholipids, 0.5 ... substrates Discussion The cyclopropyl group is present in number of natural products and its unusual properties have always stimulated chemists and biochemists [33,34] Biosynthesis of this structural...

Ngày tải lên: 07/03/2014, 16:20

... deuterium mass label Such measures proved unnecessary in the case of the linoleate-calendate reaction since the host yeast system used for this purpose does not biosynthesize the relevant substrate ... biosynthesis was assessed by GC-MS as described in the Experimental section The d2/d0 ratio of the linoleate isotopomers found in the cells was essentially identical to that of the starting material ... pooled hexane was washed with mL H2O and concentrated under N2 for HPLC purification, GC or GC-MS analysis GC-MS analysis of yeast lipids was performed using a Fisons VG TRIO 2000 mass spectrometer...

Ngày tải lên: 08/03/2014, 09:20

... Arabidopsis FAE1 mutants to decipher the importance of cysteine and histidine residues as possible catalytic residues of FAE1 condensing enzymes Their results have shown that cysteine 223 is essential ... condensing enzyme used 20:1-CoA more poorly as an elongation substrate Table Fatty acid composition of yeast cells expressing FAE1 condensing enzymes from different HEA Brassica speciesa Lysates from ... clones (WS-SDM1 and WS-SDM18) and the empty plasmid control (pYES2.1/V5-His-TOPO) in yeast cells The results of fatty acid analyses of transformed yeast cell lysates by GC of the FAMEs revealed...

Ngày tải lên: 08/03/2014, 09:20

... of that tissue This nomenclature is confusing because different types of FABP have been isolated from the same tissue, and even some orthologous FABPs from different species show distinctly different ... fish dissection, RNA isolation and RT-PCR, and Santhosh Karanth for providing technical assistance during the course of this study This work was supported by research grants from the Natural Sciences ... fabp10b transcripts in muscle and heart suggests that fabp10b may play an important role in lipid homeostasis in these tissues In contrast with zebrafish fabp10b, fabp10a transcripts were detected...

Ngày tải lên: 16/03/2014, 00:20